Restriction map for
pFN21AB7769
(Circular) (Six-base) MAPSORT of: pFN21AB7769.seq Check: 5048 from: 1 to: 6055
pFN21AB7769 6055 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2962 2962
Size: 6055
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2384 3199 4118 5502 5770 1632
Size: 752 815 919 1384 268 1917
Fragments arranged by size:
1917 1384 919 815 752 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 3000 4954 5931 1203
Size: 1797 1954 977 1327
Fragments arranged by size:
1954 1797 1327 977
AgeI A'CCGG_T
Cuts at: 2652 4886 2652
Size: 2234 3821
AhdI GACnn_n'nnGTC
Cuts at: 3034 3307 4784 3034
Size: 273 1477 4305
Fragments arranged by size:
4305 1477 273
AleI CACnn'nnGTG
Cuts at: 1166 3069 1166
Size: 1903 4152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2813 1184
Size: 1629 4426
AlwNI CAG_nnn'CTG
Cuts at: 2167 2200 2227 3285 3798 5370 2167
Size: 33 27 1058 513 1572 2852
Fragments arranged by size:
2852 1572 1058 513 33 27
ApaI G_GGCC'C
Cuts at: 2861 2861
Size: 6055
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 3364 3590 3742 5851 1493
Size: 1871 226 152 2109 1697
Fragments arranged by size:
2109 1871 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 2243 2803 3091 3380 1870
Size: 73 181 119 560 288 289 4545
Fragments arranged by size:
4545 560 289 288 181 119 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2536 2577 3376 4831 618
Size: 325 217 627 749 41 799 1455 1842
Fragments arranged by size:
1842 1455 799 749 627 325 217 41
BanII G_rGCy'C
Cuts at: 1391 2417 2514 2861 3374 1391
Size: 1026 97 347 513 4072
Fragments arranged by size:
4072 1026 513 347 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2399 928
Size: 971 500 4584
Fragments arranged by size:
4584 971 500
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2082 2116 4246 4280 1903
Size: 34 50 34 61 34 2130 34 3678
Fragments arranged by size:
3678 2130 61 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3258 4666 136
Size: 107 122 71 631 605 1586 1408 1525
Fragments arranged by size:
1586 1525 1408 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 2489 3472 2489
Size: 983 5072
Bme1580I G_kGCm'C
Cuts at: 1790 2234 2580 2861 2940 4116 5272 1790
Size: 444 346 281 79 1176 1156 2573
Fragments arranged by size:
2573 1176 1156 444 346 281 79
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2683 2743 3786
Size: 416 53 83 399 24 1266 60 1043
Cuts at: 3786 4744 442
Size: 958 1753
Fragments arranged by size:
1753 1266 1043 958 416 399 83 60
53 24
BmtI G_CTAG'C
Cuts at: 1056 3101 1056
Size: 2045 4010
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2237 2527 2708 2990 4715 1239
Size: 983 15 290 181 282 1725 2579
Fragments arranged by size:
2579 1725 983 290 282 181 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 4180 5045 5343 2081
Size: 2099 865 298 2793
Fragments arranged by size:
2793 2099 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2045 2713 4718 882
Size: 617 546 668 2005 2219
Fragments arranged by size:
2219 2005 668 617 546
BsaAI yAC'GTr
Cuts at: 493 1204 3003 3846 493
Size: 711 1799 843 2702
Fragments arranged by size:
2702 1799 843 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2784 3268 3862 4244
Size: 53 83 186 936 1251 484 594 382
Cuts at: 4244 4879 275
Size: 635 1451
Fragments arranged by size:
1451 1251 936 635 594 484 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2652 3057 4488 4886 5160 5307 5718 2652
Size: 405 1431 398 274 147 411 2989
Fragments arranged by size:
2989 1431 411 405 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2224 2254 2870 2900 3191
Size: 30 165 30 821 30 616 30 291
Cuts at: 3191 3221 1178
Size: 30 4012
Fragments arranged by size:
4012 821 616 291 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2303 2522 2648 3212 1186
Size: 892 225 219 126 564 4029
Fragments arranged by size:
4029 892 564 225 219 126
BseYI C'CCAG_C
Cuts at: 2758 2844 5258 5754 2758
Size: 86 2414 496 3059
Fragments arranged by size:
3059 2414 496 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3104 3229 1163
Size: 573 1368 125 3989
Fragments arranged by size:
3989 1368 573 125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3344 3505 4266 5294 1540
Size: 275 186 1343 161 761 1028 2301
Fragments arranged by size:
2301 1343 1028 761 275 186 161
BsiHKAI G_wGCw'C
Cuts at: 2379 4116 4201 5272 2379
Size: 1737 85 1071 3162
Fragments arranged by size:
3162 1737 1071 85
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
BsmBI CGTCTCn'nnnn_
Cuts at: 2954 2954
Size: 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2379 2417 2514 2580 2861 2940
Size: 399 444 145 38 97 66 281 79
Cuts at: 2940 3374 4116 4201 5272 1391
Size: 434 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 444 434 399 281 145
97 85 79 66 38
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2108 2962 3410 844
Size: 1264 854 448 3489
Fragments arranged by size:
3489 1264 854 448
BsrBI CCG'CTC
Cuts at: 1394 1823 3942 1394
Size: 429 2119 3507
Fragments arranged by size:
3507 2119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2652 2866 2974 3339 4699 4886 1700
Size: 110 842 214 108 365 1360 187 2869
Fragments arranged by size:
2869 1360 842 365 214 187 110 108
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 3067 4115 5127 3067
Size: 1048 1012 3995
Fragments arranged by size:
3995 1048 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2374 794
Size: 1580 4475
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 2718 1184
Size: 1534 4521
BstYI r'GATC_y
Cuts at: 1972 2247 3385 3752 4137 4154 5606 5692 5704
Size: 275 1138 367 385 17 1452 86 12
Cuts at: 5704 6050 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1138 385 367 346 275 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2175 2286 2425 2571 2175
Size: 111 139 146 5659
Fragments arranged by size:
5659 146 139 111
BtgI C'CryG_G
Cuts at: 513 1065 2581 3019 513
Size: 552 1516 438 3549
Fragments arranged by size:
3549 1516 552 438
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2696 487
Size: 683 56 1470 3846
Fragments arranged by size:
3846 1470 683 56
BtsI GCAGTG_nn'
Cuts at: 2234 2503 3017 3286 3571 4365 4385 2234
Size: 269 514 269 285 794 20 3904
Fragments arranged by size:
3904 794 514 285 269 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2437 5062 809
Size: 507 1121 2625 1802
Fragments arranged by size:
2625 1802 1121 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2273 3016 3289
Size: 54 1425 50 275 18 443 743 273
Cuts at: 3289 3341 3502 4391 6020 8
Size: 52 161 889 1629 43
Fragments arranged by size:
1629 1425 889 743 443 275 273 161
54 52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3341 3502 1537
Size: 275 1529 161 4090
Fragments arranged by size:
4090 1529 275 161
EarI CTCTTCn'nnn_
Cuts at: 1353 3985 1353
Size: 2632 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 5016 5162 387
Size: 1064 138 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2237 2384 2527 2708 2990 3199
Size: 393 590 15 147 143 181 282 209
Cuts at: 3199 4118 4715 5502 5770 1239
Size: 919 597 787 268 1524
Fragments arranged by size:
1524 919 787 597 590 393 282 268
209 181 147 143 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2448 2366
Size: 82 5973
EcoO109I rG'GnC_Cy
Cuts at: 2021 2120 2453 2857 3176 2021
Size: 99 333 404 319 4900
Fragments arranged by size:
4900 404 333 319 99
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 5202 1678
Size: 3524 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2806 2838 2870 2902 3020
Size: 32 135 32 1234 32 32 32 118
Cuts at: 3020 3052 3153 3185 3293 3325 4696 4728 4770
Size: 32 101 32 108 32 1371 32 42
Cuts at: 4770 4802 1373
Size: 32 2626
Fragments arranged by size:
2626 1371 1234 135 118 108 101 42
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3399 3645 669
Size: 988 281 1461 246 3079
Fragments arranged by size:
3079 1461 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5169 5353 1305
Size: 3864 184 2007
Fragments arranged by size:
3864 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4233
Fragments arranged by size:
4233 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3069 4013 4372 4531 518
Size: 648 1903 944 359 159 2042
Fragments arranged by size:
2042 1903 944 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2272 2297 2493 3021 3219 3232 3265 3316
Size: 666 25 196 528 198 13 33 51
Cuts at: 3316 3471 4148 5296 5541 1606
Size: 155 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 528 245 198 196
155 51 33 25 13
NaeI GCC'GGC
Cuts at: 2868 2976 2868
Size: 108 5947
NcoI C'CATG_G
Cuts at: 513 1065 2581 513
Size: 552 1516 3987
Fragments arranged by size:
3987 1516 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NgoMIV G'CCGG_C
Cuts at: 2866 2974 2866
Size: 108 5947
NheI G'CTAG_C
Cuts at: 1052 3097 1052
Size: 2045 4010
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 3413 4958 1132
Size: 97 2184 1545 2229
Fragments arranged by size:
2229 2184 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2327 2444 2713 2854 2861 2921 1348
Size: 979 117 269 141 7 60 4482
Fragments arranged by size:
4482 979 269 141 117 60 7
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 1108
Size: 1005 5050
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PmlI CAC'GTG
Cuts at: 3003 3003
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2021 2120 2453 2021
Size: 99 333 5623
Fragments arranged by size:
5623 333 99
PshAI GACnn'nnGTC
Cuts at: 1613 2783 1613
Size: 1170 4885
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 2857 2857
Size: 6055
PstI C_TGCA'G
Cuts at: 830 2172 2319 2386 2498 2792 2959 3281 3407
Size: 1342 147 67 112 294 167 322 126
Cuts at: 3407 830
Size: 3478
Fragments arranged by size:
3478 1342 322 294 167 147 126 112
67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2493 3219 3232 3316 2493
Size: 726 13 84 5232
Fragments arranged by size:
5232 726 84 13
SacII CC_GC'GG
Cuts at: 3022 3022
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SanDI GG'GwC_CC
Cuts at: 2021 2021
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2168 2315 2382 2494 2788 2955 3277
Size: 219 1123 147 67 112 294 167 322
Cuts at: 3277 3403 4538 5219 5410 826
Size: 126 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 681 322 294 219 191
167 147 126 112 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3093 3382 1872
Size: 1221 289 4545
Fragments arranged by size:
4545 1221 289
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2060 4159 5060 5322 820
Size: 197 926 117 2099 901 262 1553
Fragments arranged by size:
2099 1553 926 901 262 197 117
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 2255 1517
Size: 738 5317
StyI C'CwwG_G
Cuts at: 513 1065 2187 2581 3494 513
Size: 552 1122 394 913 3074
Fragments arranged by size:
3074 1122 913 552 394
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2248 2631 2635 4082 4099 4252 4437
Size: 1255 668 383 4 1447 17 153 185
Cuts at: 4437 4882 325
Size: 445 1498
Fragments arranged by size:
1498 1447 1255 668 445 383 185 153
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 4301 6013
Size: 274 80 33 51 1443 693 1631 1712
Cuts at: 6013 96
Size: 138
Fragments arranged by size:
1712 1631 1443 693 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2224 2256 2517 2549 3191 3223 6015 6047 2224
Size: 32 261 32 642 32 2792 32 2232
Fragments arranged by size:
2792 2232 642 261 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3091 3380 1870
Size: 1221 289 4545
Fragments arranged by size:
4545 1221 289
XmnI GAAnn'nnTTC
Cuts at: 4184 4184
Size: 6055
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI
SrfI SwaI XcmI