Restriction map for
pFN21AB7767
(Circular) (Six-base) MAPSORT of: pFN21AB7767.seq Check: 2254 from: 1 to: 6046
pFN21AB7767 6046 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3052 3052
Size: 6046
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2817 3856 278
Size: 53 83 186 936 1281 1039 2468
Fragments arranged by size:
2468 1281 1039 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3389 1937
Size: 1452 4594
Acc65I G'GTAC_C
Cuts at: 2081 3367 2081
Size: 1286 4760
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4109 5493 5761 1632
Size: 2477 1384 268 1917
Fragments arranged by size:
2477 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 4945 5922 1203
Size: 3742 977 1327
Fragments arranged by size:
3742 1327 977
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2049 3262 3789 5361 2049
Size: 1213 527 1572 2734
Fragments arranged by size:
2734 1572 1213 527
ApaI G_GGCC'C
Cuts at: 2120 2514 2933 3094 3316 2120
Size: 394 419 161 222 4850
Fragments arranged by size:
4850 419 394 222 161
ApaLI G'TGCA_C
Cuts at: 2759 4103 5259 2759
Size: 1344 1156 3546
Fragments arranged by size:
3546 1344 1156
ApoI r'AATT_y
Cuts at: 1493 2834 3157 3355 3581 3733 5842 1493
Size: 1341 323 198 226 152 2109 1697
Fragments arranged by size:
2109 1697 1341 323 226 198 152
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2664 2801 3371 1870
Size: 73 73 648 137 570 4545
Fragments arranged by size:
4545 648 570 137 73 73
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2675 2748 3367 4822
Size: 325 217 627 294 594 73 619 1455
Cuts at: 4822 618
Size: 1842
Fragments arranged by size:
1842 1455 627 619 594 325 294 217
73
BanII G_rGCy'C
Cuts at: 1391 2120 2144 2514 2607 2933 3094 3316 3365
Size: 729 24 370 93 326 161 222 49
Cuts at: 3365 1391
Size: 4072
Fragments arranged by size:
4072 729 370 326 222 161 93 49
24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2288 3277 928
Size: 971 389 989 3697
Fragments arranged by size:
3697 989 971 389
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5148 3938
Size: 1210 4836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2660 4657 136
Size: 107 122 71 631 605 988 1997 1525
Fragments arranged by size:
1997 1525 988 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2823 6041 2823
Size: 3218 2828
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 2120 2514 2678 2763 2933 3094 3316 4107
Size: 330 394 164 85 170 161 222 791
Cuts at: 4107 5263 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 791 394 330 222 170 164
161 85
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2462 2935 3777
Size: 416 53 83 399 24 1045 473 842
Cuts at: 3777 4735 442
Size: 958 1753
Fragments arranged by size:
1753 1045 958 842 473 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2520 3119 3215 4706 1239
Size: 1281 599 96 1491 2579
Fragments arranged by size:
2579 1491 1281 599 96
Bpu10I CC'TnA_GC
Cuts at: 3186 3186
Size: 6046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4171 5036 5334 4171
Size: 865 298 4883
Fragments arranged by size:
4883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3298 4709 882
Size: 617 1799 1411 2219
Fragments arranged by size:
2219 1799 1411 617
BsaAI yAC'GTr
Cuts at: 493 1204 3837 493
Size: 711 2633 2702
Fragments arranged by size:
2702 2633 711
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2814 3853 4235 4870
Size: 53 83 186 936 1281 1039 382 635
Cuts at: 4870 275
Size: 1451
Fragments arranged by size:
1451 1281 1039 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4479 4877 5151 5298 5709 4479
Size: 398 274 147 411 4816
Fragments arranged by size:
4816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5821
Fragments arranged by size:
5821 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2957 1186
Size: 904 867 4275
Fragments arranged by size:
4275 904 867
BseYI C'CCAG_C
Cuts at: 2021 2102 2418 2650 2780 3093 3315 5249 5745
Size: 81 316 232 130 313 222 1934 496
Cuts at: 5745 2021
Size: 2322
Fragments arranged by size:
2322 1934 496 316 313 232 222 130
81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3256 1163
Size: 573 1520 3953
Fragments arranged by size:
3953 1520 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2197 3496 4257 5285 1540
Size: 275 186 196 1299 761 1028 2301
Fragments arranged by size:
2301 1299 1028 761 275 196 186
BsiHKAI G_wGCw'C
Cuts at: 2763 4107 4192 5263 2763
Size: 1344 85 1071 3546
Fragments arranged by size:
3546 1344 1071 85
BsmI GAATG_Cn'
Cuts at: 3557 3650 3557
Size: 93 5953
BsmBI CGTCTCn'nnnn_
Cuts at: 3074 3074
Size: 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2144 2514 2607 2678 2763 2933
Size: 399 330 24 370 93 71 85 170
Cuts at: 2933 3094 3316 3365 4107 4192 5263 1391
Size: 161 222 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 330 222 170
161 93 85 85 71 49 24
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 3935 5665 3935
Size: 1730 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3052 3401 844
Size: 2208 349 3489
Fragments arranged by size:
3489 2208 349
BsrBI CCG'CTC
Cuts at: 1394 1823 2800 3933 1394
Size: 429 977 1133 3507
Fragments arranged by size:
3507 1133 977 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 4715 65
Size: 1183 3293 174 1396
Fragments arranged by size:
3293 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2084 2954 4690 4877 1700
Size: 110 274 870 1736 187 2869
Fragments arranged by size:
2869 1736 870 274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6046
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 4106 5118 4106
Size: 1012 5034
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3172 794
Size: 2378 3668
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6046
BstYI r'GATC_y
Cuts at: 1972 2504 2823 3376 3743 4128 4145 5597 5683
Size: 532 319 553 367 385 17 1452 86
Cuts at: 5683 5695 6041 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 553 532 385 367 346 319
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2469 2469
Size: 6046
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4555
Fragments arranged by size:
4555 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2628 487
Size: 683 56 1402 3905
Fragments arranged by size:
3905 1402 683 56
BtsI GCAGTG_nn'
Cuts at: 2065 2077 2098 2773 2977 3562 4356 4376 2065
Size: 12 21 675 204 585 794 20 3735
Fragments arranged by size:
3735 794 675 585 204 21 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2194 3493
Size: 54 1425 50 275 18 345 19 1299
Cuts at: 3493 4382 6011 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1299 889 345 275 54 50
43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2194 3493 1537
Size: 275 382 1299 4090
Fragments arranged by size:
4090 1299 382 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3976 1353
Size: 2623 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2930 4633 5007 5153 387
Size: 1064 138 1341 1703 374 146 1280
Fragments arranged by size:
1703 1341 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2520 3119 3215 4109 4706 5493 5761
Size: 393 888 599 96 894 597 787 268
Cuts at: 5761 1239
Size: 1524
Fragments arranged by size:
1524 894 888 787 599 597 393 268
96
EcoO109I rG'GnC_Cy
Cuts at: 2116 2170 2456 2511 2668 2929 3312 2116
Size: 54 286 55 157 261 383 4850
Fragments arranged by size:
4850 383 286 261 157 55 54
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 2795 5193 1678
Size: 1117 2398 2531
Fragments arranged by size:
2531 2398 1117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2221 2253 2275 2307 4687
Size: 32 135 32 649 32 22 32 2380
Cuts at: 4687 4719 4761 4793 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2380 649 135 42 32 32 32
32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3390 3636 669
Size: 988 281 1452 246 3079
Fragments arranged by size:
3079 1452 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 2085 3371 2085
Size: 1286 4760
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2219 5160 5344 1305
Size: 914 2941 184 2007
Fragments arranged by size:
2941 2007 914 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 10
Size: 54 1425 343 345 3879
Fragments arranged by size:
3879 1425 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4004 4363 4522 518
Size: 648 2838 359 159 2042
Fragments arranged by size:
2838 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2692 3097 3181 3462 4139 5287 5532
Size: 440 646 405 84 281 677 1148 245
Cuts at: 5532 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 646 440 405 281 245
84
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4555
Fragments arranged by size:
4555 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 2194 3493 2194
Size: 1299 4747
NspI r_CATG'y
Cuts at: 1132 1229 3404 4949 1132
Size: 97 2175 1545 2229
Fragments arranged by size:
2229 2175 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2594 1348
Size: 1246 4800
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 2398 1108
Size: 1215 75 4756
Fragments arranged by size:
4756 1215 75
PfoI T'CCnGG_A
Cuts at: 2163 3210 2163
Size: 1047 4999
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3252 3284 4115 4147 5628 5660 3252
Size: 32 831 32 1481 32 3638
Fragments arranged by size:
3638 1481 831 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2456 2456
Size: 6046
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PspOMI G'GGCC_C
Cuts at: 2116 2510 2929 3090 3312 2116
Size: 394 419 161 222 4850
Fragments arranged by size:
4850 419 394 222 161
PstI C_TGCA'G
Cuts at: 830 2445 3398 830
Size: 1615 953 3478
Fragments arranged by size:
3478 1615 953
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
PvuII CAG'CTG
Cuts at: 2692 3097 3181 2692
Size: 405 84 5557
Fragments arranged by size:
5557 405 84
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SanDI GG'GwC_CC
Cuts at: 2456 2456
Size: 6046
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SexAI A'CCwGG_T
Cuts at: 2367 3150 2367
Size: 783 5263
SfcI C'TryA_G
Cuts at: 826 1045 2441 3394 4529 5210 5401 826
Size: 219 1396 953 1135 681 191 1471
Fragments arranged by size:
1471 1396 1135 953 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 2018 3373 1872
Size: 146 1355 4545
Fragments arranged by size:
4545 1355 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2801 4150 5051 5313 820
Size: 197 926 858 1349 901 262 1553
Fragments arranged by size:
1553 1349 926 901 858 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 2474 1517
Size: 957 5089
StyI C'CwwG_G
Cuts at: 513 1065 2004 2376 3039 3485 513
Size: 552 939 372 663 446 3074
Fragments arranged by size:
3074 939 663 552 446 372
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3269 4073 4090 4243 4428 4873 325
Size: 1255 1689 804 17 153 185 445 1498
Fragments arranged by size:
1689 1498 1255 804 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4292 6004 96
Size: 274 80 33 51 1443 2315 1712 138
Fragments arranged by size:
2315 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2044 2076 6006 6038 2044
Size: 32 3930 32 2052
Fragments arranged by size:
3930 2052 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2495 3232 2495
Size: 737 5309
XhoI C'TCGA_G
Cuts at: 1943 2801 1943
Size: 858 5188
XmaI C'CCGG_G
Cuts at: 1870 2016 3371 1870
Size: 146 1355 4545
Fragments arranged by size:
4545 1355 146
XmnI GAAnn'nnTTC
Cuts at: 2540 4175 2540
Size: 1635 4411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2815 3854 276
Size: 53 83 186 936 1281 1039 2468
Fragments arranged by size:
2468 1281 1039 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SfiI SfoI SrfI SwaI