Restriction map for
pFN21AB7766
(Circular) (Six-base) MAPSORT of: pFN21AB7766.seq Check: 9805 from: 1 to: 6214
pFN21AB7766 6214 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2323 3557 1937
Size: 386 1234 4594
Fragments arranged by size:
4594 1234 386
Acc65I G'GTAC_C
Cuts at: 3535 3535
Size: 6214
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4277 5661 5929 1632
Size: 2645 1384 268 1917
Fragments arranged by size:
2645 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 5113 6090 1203
Size: 3910 977 1327
Fragments arranged by size:
3910 1327 977
AgeI A'CCGG_T
Cuts at: 3390 5045 3390
Size: 1655 4559
AhdI GACnn_n'nnGTC
Cuts at: 4943 4943
Size: 6214
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 3957 5529 3957
Size: 1572 4642
ApaLI G'TGCA_C
Cuts at: 4271 5427 4271
Size: 1156 5058
ApoI r'AATT_y
Cuts at: 1493 2039 2083 2194 2332 2471 2479 2583 2748
Size: 546 44 111 138 139 8 104 165
Cuts at: 2748 2792 2832 2916 3000 3185 3252 3336 3523
Size: 44 40 84 84 185 67 84 187
Cuts at: 3523 3749 3901 6010 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 546 226 187 185 165 152
139 138 111 104 84 84 84 67
44 44 40 8
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 3539 1870
Size: 73 1596 4545
Fragments arranged by size:
4545 1596 73
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3535 4990 618
Size: 325 217 627 1748 1455 1842
Fragments arranged by size:
1842 1748 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3533 1391
Size: 2142 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 2207 928
Size: 971 220 88 4935
Fragments arranged by size:
4935 971 220 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 1903
Size: 34 2468 34 3678
Fragments arranged by size:
3678 2468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 2160 1548
Size: 612 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4825 136
Size: 107 122 71 631 605 3153 1525
Fragments arranged by size:
3153 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 4275 5431 1790
Size: 2485 1156 2573
Fragments arranged by size:
2573 2485 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2222 3945 4903
Size: 416 53 83 399 24 805 1723 958
Cuts at: 4903 442
Size: 1753
Fragments arranged by size:
1753 1723 958 805 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2192 2613 2778 2862 3114 3282 3366
Size: 830 123 421 165 84 252 168 84
Cuts at: 3366 4874 1239
Size: 1508 2579
Fragments arranged by size:
2579 1508 830 421 252 168 165 123
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4339 5204 5502 4339
Size: 865 298 5051
Fragments arranged by size:
5051 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4877 882
Size: 617 3378 2219
Fragments arranged by size:
3378 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4005 493
Size: 711 2801 2702
Fragments arranged by size:
2801 2702 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4021 4403 5038 275
Size: 53 83 186 936 2488 382 635 1451
Fragments arranged by size:
2488 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3390 4647 5045 5319 5466 5877 3390
Size: 1257 398 274 147 411 3727
Fragments arranged by size:
3727 1257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5989
Fragments arranged by size:
5989 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 5334
BseYI C'CCAG_C
Cuts at: 5417 5913 5417
Size: 496 5718
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5641
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4275 4360 5431 4275
Size: 85 1071 5058
Fragments arranged by size:
5058 1071 85
BsmI GAATG_Cn'
Cuts at: 2553 3725 3818 2553
Size: 1172 93 4949
Fragments arranged by size:
4949 1172 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3533 4275 4360 5431 1391
Size: 399 1743 742 85 1071 2174
Fragments arranged by size:
2174 1743 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3569 844
Size: 2725 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2066 4709 4883 65
Size: 1183 818 2643 174 1396
Fragments arranged by size:
2643 1396 1183 818 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3390 4858 5045 1700
Size: 110 1580 1468 187 2869
Fragments arranged by size:
2869 1580 1468 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 4274 5286 4274
Size: 1012 5202
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6214
BstBI TT'CG_AA
Cuts at: 2510 5018 2510
Size: 2508 3706
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6214
BstYI r'GATC_y
Cuts at: 1972 3544 3911 4296 4313 5765 5851 5863 6209
Size: 1572 367 385 17 1452 86 12 346
Cuts at: 6209 1972
Size: 1977
Fragments arranged by size:
1977 1572 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4723
Fragments arranged by size:
4723 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 3730 4524 4544 3730
Size: 794 20 5400
Fragments arranged by size:
5400 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2261 3661
Size: 54 1425 50 275 18 201 230 1400
Cuts at: 3661 4550 6179 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1400 889 275 230 201 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4144 1353
Size: 2791 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4801 5175 5321 387
Size: 1064 138 3212 374 146 1280
Fragments arranged by size:
3212 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2192 2613 2778 2862 3114 3282
Size: 393 437 123 421 165 84 252 168
Cuts at: 3282 3366 4277 4874 5661 5929 1239
Size: 84 911 597 787 268 1524
Fragments arranged by size:
1524 911 787 597 437 421 393 268
252 168 165 123 84 84
EcoRI G'AATT_C
Cuts at: 2039 2748 2832 3000 3252 3336 3523 3901 2039
Size: 709 84 168 252 84 187 378 4352
Fragments arranged by size:
4352 709 378 252 187 168 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2523 2523
Size: 6214
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 5361 1678
Size: 3683 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 4855 4887 4929
Size: 32 135 32 580 32 2671 32 42
Cuts at: 4929 4961 1373
Size: 32 2626
Fragments arranged by size:
2671 2626 580 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2016 3558 3804 669
Size: 988 281 78 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246 78
HindIII A'AGCT_T
Cuts at: 748 2961 3045 3129 3381 748
Size: 2213 84 84 252 3581
Fragments arranged by size:
3581 2213 252 84 84
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KpnI G_GTAC'C
Cuts at: 3539 3539
Size: 6214
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5328 5512 1305
Size: 4023 184 2007
Fragments arranged by size:
4023 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2033 2263 10
Size: 54 1425 343 201 230 3961
Fragments arranged by size:
3961 1425 343 230 201 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2941 4172 4531 4690 518
Size: 648 1775 1231 359 159 2042
Fragments arranged by size:
2042 1775 1231 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3630 4307 5455 5700 1606
Size: 2024 677 1148 245 2120
Fragments arranged by size:
2120 2024 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4723
Fragments arranged by size:
4723 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 3572 5117 1132
Size: 97 2343 1545 2229
Fragments arranged by size:
2343 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6214
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6214
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 2394 3784 2394
Size: 1390 4824
PstI C_TGCA'G
Cuts at: 830 3566 830
Size: 2736 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SexAI A'CCwGG_T
Cuts at: 2121 2121
Size: 6214
SfcI C'TryA_G
Cuts at: 826 1045 2113 3177 3562 4697 5378 5569 826
Size: 219 1068 1064 385 1135 681 191 1471
Fragments arranged by size:
1471 1135 1068 1064 681 385 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4318 5219 5481 820
Size: 197 926 2375 901 262 1553
Fragments arranged by size:
2375 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 2436 4138 5
Size: 47 2384 1702 2081
Fragments arranged by size:
2384 2081 1702 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6214
StyI C'CwwG_G
Cuts at: 513 1065 2004 2189 3653 513
Size: 552 939 185 1464 3074
Fragments arranged by size:
3074 1464 939 552 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4241 4258 4411 4596 5041 325
Size: 1255 2661 17 153 185 445 1498
Fragments arranged by size:
2661 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2651 4460 6172
Size: 274 80 33 51 1443 674 1809 1712
Cuts at: 6172 96
Size: 138
Fragments arranged by size:
1809 1712 1443 674 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6174 6206 6174
Size: 32 6182
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6214
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6214
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 2519 4343 2519
Size: 1824 4390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI