Restriction map for
pFN21AB7765
(Circular) (Six-base) MAPSORT of: pFN21AB7765.seq Check: 9804 from: 1 to: 6055
pFN21AB7765 6055 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2544 3398 1937
Size: 607 854 4594
Fragments arranged by size:
4594 854 607
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2760 4118 5502 5770 1632
Size: 1128 1358 1384 268 1917
Fragments arranged by size:
1917 1384 1358 1128 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 4954 5931 1203
Size: 3751 977 1327
Fragments arranged by size:
3751 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 4784 4784
Size: 6055
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AlwNI CAG_nnn'CTG
Cuts at: 2203 2557 2687 2738 3076 3798 5370 2203
Size: 354 130 51 338 722 1572 2888
Fragments arranged by size:
2888 1572 722 354 338 130 51
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2975 3086 3364 3590 3742 5851 1493
Size: 1482 111 278 226 152 2109 1697
Fragments arranged by size:
2109 1697 1482 278 226 152 111
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 3380 1870
Size: 73 1437 4545
Fragments arranged by size:
4545 1437 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2284 2361 3376 4831 618
Size: 325 217 627 497 77 1015 1455 1842
Fragments arranged by size:
1842 1455 1015 627 497 325 217 77
BanII G_rGCy'C
Cuts at: 1391 2505 3374 1391
Size: 1114 869 4072
Fragments arranged by size:
4072 1114 869
BbeI G_GCGC'C
Cuts at: 2288 2365 2288
Size: 77 5978
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2665 928
Size: 971 766 4318
Fragments arranged by size:
4318 971 766
BbvCI CC'TCA_GC
Cuts at: 2705 2705
Size: 6055
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4246 4280 1903
Size: 34 2309 34 3678
Fragments arranged by size:
3678 2309 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2370 4666 136
Size: 107 122 71 631 605 698 2296 1525
Fragments arranged by size:
2296 1525 698 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 4116 5272 1790
Size: 2326 1156 2573
Fragments arranged by size:
2573 2326 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2209 2798 3137
Size: 416 53 83 399 24 792 589 339
Cuts at: 3137 3786 4744 442
Size: 649 958 1753
Fragments arranged by size:
1753 958 792 649 589 416 399 339
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2816 2816
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 2417 3315 3350 4715 1239
Size: 933 245 898 35 1365 2579
Fragments arranged by size:
2579 1365 933 898 245 35
Bpu10I CC'TnA_GC
Cuts at: 2705 2705
Size: 6055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4180 5045 5343 4180
Size: 865 298 4892
Fragments arranged by size:
4892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3291 4718 882
Size: 617 1792 1427 2219
Fragments arranged by size:
2219 1792 1427 617
BsaAI yAC'GTr
Cuts at: 493 1204 3846 493
Size: 711 2642 2702
Fragments arranged by size:
2702 2642 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2285 2362 3862 4244
Size: 53 83 186 936 752 77 1500 382
Cuts at: 4244 4879 275
Size: 635 1451
Fragments arranged by size:
1500 1451 936 752 635 382 186 83
77 53
BsaWI w'CCGG_w
Cuts at: 3111 4488 4886 5160 5307 5718 3111
Size: 1377 398 274 147 411 3448
Fragments arranged by size:
3448 1377 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 2423 2453 1178
Size: 30 165 30 684 30 306 30 4780
Fragments arranged by size:
4780 684 306 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2092 2693 1186
Size: 906 601 4548
Fragments arranged by size:
4548 906 601
BseYI C'CCAG_C
Cuts at: 3069 5258 5754 3069
Size: 2189 496 3370
Fragments arranged by size:
3370 2189 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 2549 2555 1163
Size: 573 522 291 6 4663
Fragments arranged by size:
4663 573 522 291 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2223 2320 2370 2877 3505 4266
Size: 275 186 222 97 50 507 628 761
Cuts at: 4266 5294 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 628 507 275 222 186
97 50
BsiHKAI G_wGCw'C
Cuts at: 4116 4201 5272 4116
Size: 85 1071 4899
Fragments arranged by size:
4899 1071 85
BsiWI C'GTAC_G
Cuts at: 2250 2250
Size: 6055
BsmI GAATG_Cn'
Cuts at: 2774 3566 3659 2774
Size: 792 93 5170
Fragments arranged by size:
5170 792 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3146 3146
Size: 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2505 3374 4116 4201 5272 1391
Size: 399 715 869 742 85 1071 2174
Fragments arranged by size:
2174 1071 869 742 715 399 85
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 3944 5674 3944
Size: 1730 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 3410 844
Size: 1610 956 3489
Fragments arranged by size:
3489 1610 956
BsrBI CCG'CTC
Cuts at: 1394 1823 2339 2439 2894 3942 1394
Size: 429 516 100 455 1048 3507
Fragments arranged by size:
3507 1048 516 455 429 100
BsrDI GCAATG_nn'
Cuts at: 65 1248 2080 4550 4724 65
Size: 1183 832 2470 174 1396
Fragments arranged by size:
2470 1396 1183 832 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2281 2369 2829 4699 4886 1700
Size: 110 471 88 460 1870 187 2869
Fragments arranged by size:
2869 1870 471 460 187 110 88
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2560 794
Size: 1766 4289
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstXI CCAn_nnnn'nTGG
Cuts at: 3203 3203
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 3385 3752 4137 4154 5606 5692 5704 6050
Size: 1413 367 385 17 1452 86 12 346
Cuts at: 6050 1972
Size: 1977
Fragments arranged by size:
1977 1452 1413 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2084 2356 2478 3121 3196 3225 513
Size: 552 1019 272 122 643 75 29 3343
Fragments arranged by size:
3343 1019 643 552 272 122 75 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 3571 4365 4385 3571
Size: 794 20 5241
Fragments arranged by size:
5241 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 2317 2367
Size: 54 1425 50 275 18 390 97 50
Cuts at: 2367 2487 2874 3502 4391 6020 8
Size: 120 387 628 889 1629 43
Fragments arranged by size:
1629 1425 889 628 390 387 275 120
97 54 50 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2220 2317 2367 2874 3502 1537
Size: 275 408 97 50 507 628 4090
Fragments arranged by size:
4090 628 507 408 275 97 50
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 2800 3985 1353
Size: 681 766 1185 3423
Fragments arranged by size:
3423 1185 766 681
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 2899 4642 5016 5162 387
Size: 1064 138 506 804 1743 374 146 1280
Fragments arranged by size:
1743 1280 1064 804 506 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2417 2760 3315 3350 4118 4715
Size: 393 540 245 343 555 35 768 597
Cuts at: 4715 5502 5770 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 768 597 555 540 393 343
268 245 35
EcoO109I rG'GnC_Cy
Cuts at: 2089 2591 2089
Size: 502 5553
EcoRI G'AATT_C
Cuts at: 3086 3364 3742 3086
Size: 278 378 5399
Fragments arranged by size:
5399 378 278
FseI GG_CCGG'CC
Cuts at: 2373 2373
Size: 6055
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 2288 2365 2418 5202 1678
Size: 610 77 53 2784 2531
Fragments arranged by size:
2784 2531 610 77 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2216 2248 2685 2717 4696
Size: 32 135 32 644 32 437 32 1979
Cuts at: 4696 4728 4770 4802 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1979 644 437 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2545 3399 3645 669
Size: 988 281 607 854 246 3079
Fragments arranged by size:
3079 988 854 607 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KasI G'GCGC_C
Cuts at: 2284 2361 2284
Size: 77 5978
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2466 2829 5169 5353 1305
Size: 1161 363 2340 184 2007
Fragments arranged by size:
2340 2007 1161 363 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4233
Fragments arranged by size:
4233 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2600 2767 4013 4372 4531 518
Size: 648 1434 167 1246 359 159 2042
Fragments arranged by size:
2042 1434 1246 648 359 167 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2203 2480 3227 3311 3471 4148 5296
Size: 480 117 277 747 84 160 677 1148
Cuts at: 5296 5541 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 747 677 480 277 245 160
117 84
NaeI GCC'GGC
Cuts at: 2283 2371 2283
Size: 88 5967
NarI GG'CG_CC
Cuts at: 2285 2362 2285
Size: 77 5978
NcoI C'CATG_G
Cuts at: 513 1065 3121 513
Size: 552 2056 3447
Fragments arranged by size:
3447 2056 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NgoMIV G'CCGG_C
Cuts at: 2281 2369 2281
Size: 88 5967
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 2515 3058 3413 4958 1132
Size: 97 1286 543 355 1545 2229
Fragments arranged by size:
2229 1545 1286 543 355 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2203 1108
Size: 902 193 4960
Fragments arranged by size:
4960 902 193
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2591 2089
Size: 502 5553
PshAI GACnn'nnGTC
Cuts at: 1613 3303 1613
Size: 1690 4365
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PstI C_TGCA'G
Cuts at: 830 3243 3407 830
Size: 2413 164 3478
Fragments arranged by size:
3478 2413 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2203 3311 2203
Size: 1108 4947
SacII CC_GC'GG
Cuts at: 2087 2481 3228 2087
Size: 394 747 4914
Fragments arranged by size:
4914 747 394
SalI G'TCGA_C
Cuts at: 1936 2543 3397 1936
Size: 607 854 4594
Fragments arranged by size:
4594 854 607
SanDI GG'GwC_CC
Cuts at: 2089 2089
Size: 6055
SapI GCTCTTCn'nnn_
Cuts at: 2800 2800
Size: 6055
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 2122 3239 3403 4538 5219 5410 826
Size: 219 1077 1117 164 1135 681 191 1471
Fragments arranged by size:
1471 1135 1117 1077 681 219 191 164
SfoI GGC'GCC
Cuts at: 2286 2363 2286
Size: 77 5978
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4159 5060 5322 820
Size: 197 926 2216 901 262 1553
Fragments arranged by size:
2216 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3002 3979 5
Size: 47 2950 977 2081
Fragments arranged by size:
2950 2081 977 47
StuI AGG'CCT
Cuts at: 1517 2309 1517
Size: 792 5263
StyI C'CwwG_G
Cuts at: 513 1065 3121 3494 513
Size: 552 2056 373 3074
Fragments arranged by size:
3074 2056 552 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4082 4099 4252 4437 4882 325
Size: 1255 2502 17 153 185 445 1498
Fragments arranged by size:
2502 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2743 4301 6013
Size: 274 80 33 51 1443 766 1558 1712
Cuts at: 6013 96
Size: 138
Fragments arranged by size:
1712 1558 1443 766 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2087 2119 6015 6047 2087
Size: 32 3896 32 2095
Fragments arranged by size:
3896 2095 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 2934 4184 2934
Size: 1250 4805
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SexAI SfiI SrfI SwaI XcmI