Restriction map for
pFN21AB7763
(Circular) (Six-base) MAPSORT of: pFN21AB7763.seq Check: 7163 from: 1 to: 6049
pFN21AB7763 6049 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2174 2174
Size: 6049
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2214 3392 1937
Size: 277 1178 4594
Fragments arranged by size:
4594 1178 277
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 2075 4176 4549 1563
Size: 512 2101 373 3063
Fragments arranged by size:
3063 2101 512 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 4112 5496 5764 1632
Size: 701 1779 1384 268 1917
Fragments arranged by size:
1917 1779 1384 701 268
AflII C'TTAA_G
Cuts at: 820 1017 3164 820
Size: 197 2147 3705
Fragments arranged by size:
3705 2147 197
AflIII A'CryG_T
Cuts at: 1203 4948 5925 1203
Size: 3745 977 1327
Fragments arranged by size:
3745 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 2672 2828 1166
Size: 1506 156 4387
Fragments arranged by size:
4387 1506 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2253 1184
Size: 1069 4980
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2910 2942 2910
Size: 32 6017
AlwNI CAG_nnn'CTG
Cuts at: 3792 5364 3792
Size: 1572 4477
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 2408 2659 2767 3358 3584 3736 5845 1493
Size: 915 251 108 591 226 152 2109 1697
Fragments arranged by size:
2109 1697 915 591 251 226 152 108
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 3374 1870
Size: 73 1431 4545
Fragments arranged by size:
4545 1431 73
BamHI G'GATC_C
Cuts at: 3379 3746 3379
Size: 367 5682
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 3370 4825 618
Size: 325 217 627 303 1280 1455 1842
Fragments arranged by size:
1842 1455 1280 627 325 303 217
BanII G_rGCy'C
Cuts at: 1391 3109 3368 1391
Size: 1718 259 4072
Fragments arranged by size:
4072 1718 259
BbeI G_GCGC'C
Cuts at: 2094 2094
Size: 6049
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3032 928
Size: 971 1133 3945
Fragments arranged by size:
3945 1133 971
BbvCI CC'TCA_GC
Cuts at: 2700 3231 2700
Size: 531 5518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2234 2268 4240 4274 1903
Size: 34 297 34 1972 34 3678
Fragments arranged by size:
3678 1972 297 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4660 136
Size: 107 122 71 631 605 2988 1525
Fragments arranged by size:
2988 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 3466 3466
Size: 6049
Bme1580I G_kGCm'C
Cuts at: 1790 4110 5266 1790
Size: 2320 1156 2573
Fragments arranged by size:
2573 2320 1156
BmgBI CAC'GTC
Cuts at: 1318 2565 1318
Size: 1247 4802
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3300 3780 4738
Size: 416 53 83 399 24 1883 480 958
Cuts at: 4738 442
Size: 1753
Fragments arranged by size:
1883 1753 958 480 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2456 2459 2898 3071 3191 4709 1239
Size: 1217 3 439 173 120 1518 2579
Fragments arranged by size:
2579 1518 1217 439 173 120 3
Bpu10I CC'TnA_GC
Cuts at: 2700 3231 2700
Size: 531 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4174 5039 5337 4174
Size: 865 298 4886
Fragments arranged by size:
4886 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4712 882
Size: 617 3213 2219
Fragments arranged by size:
3213 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3840 493
Size: 711 2636 2702
Fragments arranged by size:
2702 2636 711
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 3856 4238 4873
Size: 53 83 186 936 558 1765 382 635
Cuts at: 4873 275
Size: 1451
Fragments arranged by size:
1765 1451 936 635 558 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4482 4880 5154 5301 5712 4482
Size: 398 274 147 411 4819
Fragments arranged by size:
4819 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2376 2406 3111 3141 1178
Size: 30 165 30 973 30 705 30 4086
Fragments arranged by size:
4086 973 705 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2972 1186
Size: 1786 4263
BseYI C'CCAG_C
Cuts at: 5252 5748 5252
Size: 496 5553
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2307 1163
Size: 573 571 4905
Fragments arranged by size:
4905 573 571
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 4260 5288 1540
Size: 275 186 1498 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4110 4195 5266 4110
Size: 85 1071 4893
Fragments arranged by size:
4893 1071 85
BsmI GAATG_Cn'
Cuts at: 2302 3560 3653 2302
Size: 1258 93 4698
Fragments arranged by size:
4698 1258 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3109 3368 4110 4195 5266 1391
Size: 399 1319 259 742 85 1071 2174
Fragments arranged by size:
2174 1319 1071 742 399 259 85
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2174 2947 3404 844
Size: 1330 773 457 3489
Fragments arranged by size:
3489 1330 773 457
BsrBI CCG'CTC
Cuts at: 1394 1823 3936 1394
Size: 429 2113 3507
Fragments arranged by size:
3507 2113 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 3064 4544 4718 65
Size: 1183 1239 577 1480 174 1396
Fragments arranged by size:
1480 1396 1239 1183 577 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4693 4880 1700
Size: 110 2883 187 2869
Fragments arranged by size:
2883 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 4109 5121 4109
Size: 1012 5037
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2696 794
Size: 1902 4147
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 2627 1184
Size: 1443 4606
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 3379 3746 4131 4148 5600 5686 5698 6044
Size: 1407 367 385 17 1452 86 12 346
Cuts at: 6044 1972
Size: 1977
Fragments arranged by size:
1977 1452 1407 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2623 2623
Size: 6049
BtgI C'CryG_G
Cuts at: 513 1065 3213 3330 513
Size: 552 2148 117 3232
Fragments arranged by size:
3232 2148 552 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 3565 4359 4379 3565
Size: 794 20 5235
Fragments arranged by size:
5235 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3018 3053 529
Size: 35 2454 35 3525
Fragments arranged by size:
3525 2454 35 35
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DraIII CAC_nnn'GTG
Cuts at: 2263 2263
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2887 3496 4385
Size: 54 1425 50 275 18 1057 609 889
Cuts at: 4385 6014 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1057 889 609 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2979 3097 3216 3264 3979 1353
Size: 1626 118 119 48 715 3423
Fragments arranged by size:
3423 1626 715 119 118 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2456 2459 2898 3071 3191 4112
Size: 393 701 123 3 439 173 120 921
Cuts at: 4112 4709 5496 5764 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 921 787 701 597 439 393 268
173 123 120 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2877 2877
Size: 6049
EcoO109I rG'GnC_Cy
Cuts at: 2220 2220
Size: 6049
EcoRI G'AATT_C
Cuts at: 3358 3736 3358
Size: 378 5671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2192 2192
Size: 6049
FspI TGC'GCA
Cuts at: 2256 4555 2256
Size: 2299 3750
FspAI rTGC'GCAy
Cuts at: 2256 2256
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2094 2347 5196 1678
Size: 416 253 2849 2531
Fragments arranged by size:
2849 2531 416 253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 3111 3143 4690
Size: 32 135 32 804 32 703 32 1547
Cuts at: 4690 4722 4764 4796 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1547 804 703 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2581 2776 3393 3639 669
Size: 988 281 643 195 617 246 3079
Fragments arranged by size:
3079 988 643 617 281 246 195
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 2581 3639 2581
Size: 1058 4991
KasI G'GCGC_C
Cuts at: 2090 2090
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2745 3246 5163 5347 1305
Size: 1440 501 1917 184 2007
Fragments arranged by size:
2007 1917 1440 501 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2889 10
Size: 54 1425 343 1057 3170
Fragments arranged by size:
3170 1425 1057 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2355 2672 2828 4007 4366 4525 518
Size: 648 1189 317 156 1179 359 159 2042
Fragments arranged by size:
2042 1189 1179 648 359 317 159 156
MspA1I CmG'CkG
Cuts at: 1606 3465 4142 5290 5535 1606
Size: 1859 677 1148 245 2120
Fragments arranged by size:
2120 1859 1148 677 245
NarI GG'CG_CC
Cuts at: 2091 2091
Size: 6049
NcoI C'CATG_G
Cuts at: 513 1065 3213 3330 513
Size: 552 2148 117 3232
Fragments arranged by size:
3232 2148 552 117
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2255 2311 3407 4952 1132
Size: 97 1026 56 1096 1545 2229
Fragments arranged by size:
2229 1545 1096 1026 97 56
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2109 1108
Size: 1001 5048
PfoI T'CCnGG_A
Cuts at: 2472 3087 2472
Size: 615 5434
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2220 2220
Size: 6049
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PstI C_TGCA'G
Cuts at: 830 2242 3401 830
Size: 1412 1159 3478
Fragments arranged by size:
3478 1412 1159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SapI GCTCTTCn'nnn_
Cuts at: 3097 3097
Size: 6049
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 2172 4297 6009 2172
Size: 2125 1712 2212
Fragments arranged by size:
2212 2125 1712
SfcI C'TryA_G
Cuts at: 826 1045 2238 3074 3243 3397 4532 5213 5404
Size: 219 1193 836 169 154 1135 681 191
Cuts at: 5404 826
Size: 1471
Fragments arranged by size:
1471 1193 1135 836 681 219 191 169
154
SfoI GGC'GCC
Cuts at: 2092 2092
Size: 6049
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3164 4153 5054 5316 820
Size: 197 926 1221 989 901 262 1553
Fragments arranged by size:
1553 1221 989 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3407 1132
Size: 2275 3774
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6049
StyI C'CwwG_G
Cuts at: 513 1065 2301 3011 3213 3330 3488 513
Size: 552 1236 710 202 117 158 3074
Fragments arranged by size:
3074 1236 710 552 202 158 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4076 4093 4246 4431 4876 325
Size: 1255 2496 17 153 185 445 1498
Fragments arranged by size:
2496 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2170 2647 4295
Size: 274 80 33 51 1443 193 477 1648
Cuts at: 4295 6007 96
Size: 1712 138
Fragments arranged by size:
1712 1648 1443 477 274 193 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6009 6041 6009
Size: 32 6017
Tth111I GACn'n_nGTC
Cuts at: 1316 3292 1316
Size: 1976 4073
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2674 2011
Size: 663 5386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FseI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI