Restriction map for
pFN21AB7758
(Circular) (Six-base) MAPSORT of: pFN21AB7758.seq Check: 9932 from: 1 to: 6199
pFN21AB7758 6199 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2996 2996
Size: 6199
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2769 4009 278
Size: 53 83 186 936 1233 1240 2468
Fragments arranged by size:
2468 1240 1233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2025 3542 1937
Size: 88 1517 4594
Fragments arranged by size:
4594 1517 88
Acc65I G'GTAC_C
Cuts at: 3520 3520
Size: 6199
AclI AA'CG_TT
Cuts at: 1563 4326 4699 1563
Size: 2763 373 3063
Fragments arranged by size:
3063 2763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4262 5646 5914 1632
Size: 2630 1384 268 1917
Fragments arranged by size:
2630 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6002
AflIII A'CryG_T
Cuts at: 1203 3248 5098 6075 1203
Size: 2045 1850 977 1327
Fragments arranged by size:
2045 1850 1327 977
AgeI A'CCGG_T
Cuts at: 5030 5030
Size: 6199
AhdI GACnn_n'nnGTC
Cuts at: 4928 4928
Size: 6199
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6199
AlwNI CAG_nnn'CTG
Cuts at: 2106 2455 3942 5514 2106
Size: 349 1487 1572 2791
Fragments arranged by size:
2791 1572 1487 349
ApaLI G'TGCA_C
Cuts at: 4256 5412 4256
Size: 1156 5043
ApoI r'AATT_y
Cuts at: 1493 3001 3508 3734 3886 5995 1493
Size: 1508 507 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 507 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4753 160
Size: 872 3721 1606
Fragments arranged by size:
3721 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 3524 1870
Size: 73 1581 4545
Fragments arranged by size:
4545 1581 73
BamHI G'GATC_C
Cuts at: 3105 3529 3896 3105
Size: 424 367 5408
Fragments arranged by size:
5408 424 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3520 4975 618
Size: 325 217 627 1733 1455 1842
Fragments arranged by size:
1842 1733 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2067 2267 3155 3497 3518 1391
Size: 676 200 888 342 21 4072
Fragments arranged by size:
4072 888 676 342 200 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3028 928
Size: 971 1129 4099
Fragments arranged by size:
4099 1129 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4390 4424 1903
Size: 34 2453 34 3678
Fragments arranged by size:
3678 2453 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4091 5301 4091
Size: 1210 4989
BclI T'GATC_A
Cuts at: 1548 2145 1548
Size: 597 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4810 136
Size: 107 122 71 631 605 3138 1525
Fragments arranged by size:
3138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2319 2710 2848 6194 2319
Size: 391 138 3346 2324
Fragments arranged by size:
3346 2324 391 138
BlpI GC'TnA_GC
Cuts at: 3616 3616
Size: 6199
Bme1580I G_kGCm'C
Cuts at: 1790 3488 4260 5416 1790
Size: 1698 772 1156 2573
Fragments arranged by size:
2573 1698 1156 772
BmgBI CAC'GTC
Cuts at: 1318 2911 1318
Size: 1593 4606
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2911 3082 3235
Size: 416 53 83 399 24 1494 171 153
Cuts at: 3235 3930 4888 442
Size: 695 958 1753
Fragments arranged by size:
1753 1494 958 695 416 399 171 153
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6199
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2300 2639 2699 3184 4859 1239
Size: 842 219 339 60 485 1675 2579
Fragments arranged by size:
2579 1675 842 485 339 219 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2137 4324 5189 5487 2137
Size: 2187 865 298 2849
Fragments arranged by size:
2849 2187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4862 882
Size: 617 3363 2219
Fragments arranged by size:
3363 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3990 493
Size: 711 2786 2702
Fragments arranged by size:
2786 2702 711
BsaBI GATnn'nnATC
Cuts at: 6193 6193
Size: 6199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2766 4006 4388 5023
Size: 53 83 186 936 1233 1240 382 635
Cuts at: 5023 275
Size: 1451
Fragments arranged by size:
1451 1240 1233 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4632 5030 5304 5451 5862 4632
Size: 398 274 147 411 4969
Fragments arranged by size:
4969 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5974
Fragments arranged by size:
5974 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 3265 3268 1186
Size: 1423 656 3 4117
Fragments arranged by size:
4117 1423 656 3
BseYI C'CCAG_C
Cuts at: 2352 5402 5898 2352
Size: 3050 496 2653
Fragments arranged by size:
3050 2653 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2974 1163
Size: 573 1238 4388
Fragments arranged by size:
4388 1238 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3649 4410 5438 1540
Size: 275 186 1648 761 1028 2301
Fragments arranged by size:
2301 1648 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 2301 3155 3397 3497 4260 4345 5416 2067
Size: 234 854 242 100 763 85 1071 2850
Fragments arranged by size:
2850 1071 854 763 242 234 100 85
BsmI GAATG_Cn'
Cuts at: 3710 3803 3710
Size: 93 6106
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2267 2301 3155 3397 3488 3497
Size: 399 277 200 34 854 242 91 9
Cuts at: 3497 3518 4260 4345 5416 1391
Size: 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 854 742 399 277 242 200
91 85 34 21 9
BspEI T'CCGG_A
Cuts at: 5862 5862
Size: 6199
BspHI T'CATG_A
Cuts at: 2769 4088 5818 2769
Size: 1319 1730 3150
Fragments arranged by size:
3150 1730 1319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 2428 2996 3442 3554 844
Size: 1210 374 568 446 112 3489
Fragments arranged by size:
3489 1210 568 446 374 112
BsrBI CCG'CTC
Cuts at: 1394 1823 4086 1394
Size: 429 2263 3507
Fragments arranged by size:
3507 2263 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4694 4868 65
Size: 1183 3446 174 1396
Fragments arranged by size:
3446 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4843 5030 1700
Size: 110 3033 187 2869
Fragments arranged by size:
3033 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6199
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6199
BssSI C'ACGA_G
Cuts at: 4259 5271 4259
Size: 1012 5187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6199
BstBI TT'CG_AA
Cuts at: 2939 5003 2939
Size: 2064 4135
BstEII G'GTnAC_C
Cuts at: 1184 2041 2384 1184
Size: 857 343 4999
Fragments arranged by size:
4999 857 343
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 2059
Size: 6199
BstYI r'GATC_y
Cuts at: 1972 2319 2710 2848 3105 3529 3896 4281 4298
Size: 347 391 138 257 424 367 385 17
Cuts at: 4298 5750 5836 5848 6194 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 424 391 385 367 347 346
257 138 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2302 3388 513
Size: 552 939 298 1086 3324
Fragments arranged by size:
3324 1086 939 552 298
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5460
Fragments arranged by size:
5460 683 56
BtsI GCAGTG_nn'
Cuts at: 2121 2935 3715 4509 4529 2121
Size: 814 780 794 20 3791
Fragments arranged by size:
3791 814 794 780 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6164
DraI TTT'AAA
Cuts at: 3503 3503
Size: 6199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2672 5206 809
Size: 507 1356 2534 1802
Fragments arranged by size:
2534 1802 1356 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2226 3646
Size: 54 1425 50 275 18 265 131 1420
Cuts at: 3646 4535 6164 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1420 889 275 265 131 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 1537
Size: 275 1834 4090
Fragments arranged by size:
4090 1834 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 3061 3143 3286 4129 1353
Size: 923 785 82 143 843 3423
Fragments arranged by size:
3423 923 843 785 143 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4786 5160 5306 387
Size: 1064 138 3197 374 146 1280
Fragments arranged by size:
3197 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2065 3153 3495 2065
Size: 1088 342 4769
Fragments arranged by size:
4769 1088 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2300 2639 2699 3184 4262 4859
Size: 393 449 219 339 60 485 1078 597
Cuts at: 4859 5646 5914 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1078 787 597 485 449 393 339
268 219 60
EcoRI G'AATT_C
Cuts at: 3001 3508 3886 3001
Size: 507 378 5314
Fragments arranged by size:
5314 507 378
FspI TGC'GCA
Cuts at: 4705 4705
Size: 6199
HaeII r_GCGC'y
Cuts at: 1678 5346 1678
Size: 3668 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2062 2094 2905 2937 3263
Size: 32 135 32 490 32 811 32 326
Cuts at: 3263 3295 3384 3416 4840 4872 4914 4946 1373
Size: 32 89 32 1424 32 42 32 2626
Fragments arranged by size:
2626 1424 811 490 326 135 89 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3543 3789 669
Size: 988 281 1605 246 3079
Fragments arranged by size:
3079 1605 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6199
HpaI GTT'AAC
Cuts at: 3789 3789
Size: 6199
KpnI G_GTAC'C
Cuts at: 3524 3524
Size: 6199
MfeI C'AATT_G
Cuts at: 3798 3798
Size: 6199
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2233 5313 5497 1305
Size: 928 3080 184 2007
Fragments arranged by size:
3080 2007 928 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2097 2228 10
Size: 54 1425 343 265 131 3981
Fragments arranged by size:
3981 1425 343 265 131 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3479 4157 4516 4675 518
Size: 648 2313 678 359 159 2042
Fragments arranged by size:
2313 2042 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2169 2671 2719 3615 4292 5440 5685 1606
Size: 563 502 48 896 677 1148 245 2120
Fragments arranged by size:
2120 1148 896 677 563 502 245 48
NcoI C'CATG_G
Cuts at: 513 1065 2004 2302 513
Size: 552 939 298 4410
Fragments arranged by size:
4410 939 552 298
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6199
NotI GC'GGCC_GC
Cuts at: 3646 3646
Size: 6199
NspI r_CATG'y
Cuts at: 1132 1229 3252 3557 5102 1132
Size: 97 2023 305 1545 2229
Fragments arranged by size:
2229 2023 1545 305 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6199
PciI A'CATG_T
Cuts at: 3248 5098 3248
Size: 1850 4349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6199
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 5781 5813 4268
Size: 32 1481 32 4654
Fragments arranged by size:
4654 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2888 1613
Size: 1275 4924
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PstI C_TGCA'G
Cuts at: 830 2439 2451 2754 3551 830
Size: 1609 12 303 797 3478
Fragments arranged by size:
3478 1609 797 303 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6199
PvuII CAG'CTG
Cuts at: 2169 2671 2169
Size: 502 5697
SacI G_AGCT'C
Cuts at: 2067 3155 3497 2067
Size: 1088 342 4769
Fragments arranged by size:
4769 1088 342
SalI G'TCGA_C
Cuts at: 1936 3541 1936
Size: 1605 4594
SapI GCTCTTCn'nnn_
Cuts at: 3143 3143
Size: 6199
SbfI CC_TGCA'GG
Cuts at: 3551 3551
Size: 6199
ScaI AGT'ACT
Cuts at: 2897 4447 6159 2897
Size: 1550 1712 2937
Fragments arranged by size:
2937 1712 1550
SfcI C'TryA_G
Cuts at: 826 1045 2435 2447 2661 2750 3547 4682 5363
Size: 219 1390 12 214 89 797 1135 681
Cuts at: 5363 5554 826
Size: 191 1471
Fragments arranged by size:
1471 1390 1135 797 681 219 214 191
89 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6199
SmaI CCC'GGG
Cuts at: 1872 3526 1872
Size: 1654 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2152 4303 5204 5466 820
Size: 197 926 209 2151 901 262 1553
Fragments arranged by size:
2151 1553 926 901 262 209 197
SnaBI TAC'GTA
Cuts at: 493 3990 493
Size: 3497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6199
SphI G_CATG'C
Cuts at: 1132 3557 1132
Size: 2425 3774
SspI AAT'ATT
Cuts at: 5 52 3050 3068 4123 5
Size: 47 2998 18 1055 2081
Fragments arranged by size:
2998 2081 1055 47 18
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6199
StyI C'CwwG_G
Cuts at: 513 1065 2004 2302 2380 3291 3455 3638 513
Size: 552 939 298 78 911 164 183 3074
Fragments arranged by size:
3074 939 911 552 298 183 164 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2893 4226 4243 4396 4581 5026 325
Size: 1255 1313 1333 17 153 185 445 1498
Fragments arranged by size:
1498 1333 1313 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 4445 6157
Size: 274 80 33 51 1443 918 1550 1712
Cuts at: 6157 96
Size: 138
Fragments arranged by size:
1712 1550 1443 918 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6159 6191 6159
Size: 32 6167
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6199
XbaI T'CTAG_A
Cuts at: 3535 3535
Size: 6199
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 3524 1870
Size: 1654 4545
XmnI GAAnn'nnTTC
Cuts at: 2561 4328 2561
Size: 1767 4432
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2767 4007 276
Size: 53 83 186 936 1233 1240 2468
Fragments arranged by size:
2468 1240 1233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI