Restriction map for
pFN21AB7757
(Circular) (Six-base) MAPSORT of: pFN21AB7757.seq Check: 9269 from: 1 to: 6046
pFN21AB7757 6046 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3389 1937
Size: 1452 4594
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4109 5493 5761 1632
Size: 2477 1384 268 1917
Fragments arranged by size:
2477 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 2463 2514 4945 5922 1203
Size: 1260 51 2431 977 1327
Fragments arranged by size:
2431 1327 1260 977 51
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 2787 3230 1166
Size: 1621 443 3982
Fragments arranged by size:
3982 1621 443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2589 1184
Size: 1405 4641
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2291 2323 2291
Size: 32 6014
AlwNI CAG_nnn'CTG
Cuts at: 3789 5361 3789
Size: 1572 4474
ApaI G_GGCC'C
Cuts at: 2396 2601 2396
Size: 205 5841
ApaLI G'TGCA_C
Cuts at: 2888 3068 4103 5259 2888
Size: 180 1035 1156 3675
Fragments arranged by size:
3675 1156 1035 180
ApoI r'AATT_y
Cuts at: 1493 2956 3355 3581 3733 5842 1493
Size: 1463 399 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 399 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 2251 2435 2506 2699 3371 1870
Size: 73 308 184 71 193 672 4545
Fragments arranged by size:
4545 672 308 193 184 73 71
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2206 2630 2636 3367
Size: 325 217 627 351 68 424 6 731
Cuts at: 3367 4822 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 731 627 424 351 325 217
68 6
BanII G_rGCy'C
Cuts at: 1391 2396 2601 2679 2700 3176 3365 1391
Size: 1005 205 78 21 476 189 4072
Fragments arranged by size:
4072 1005 476 205 189 78 21
BbeI G_GCGC'C
Cuts at: 2142 2210 2634 2640 2142
Size: 68 424 6 5548
Fragments arranged by size:
5548 424 68 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2932 928
Size: 971 1033 4042
Fragments arranged by size:
4042 1033 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2435 2469 2674 2708 2923
Size: 34 50 34 414 34 205 34 215
Cuts at: 2923 2957 4237 4271 1903
Size: 34 1280 34 3678
Fragments arranged by size:
3678 1280 414 215 205 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5148 3938
Size: 1210 4836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2262 2400 4657
Size: 107 122 71 631 605 590 138 2257
Cuts at: 4657 136
Size: 1525
Fragments arranged by size:
2257 1525 631 605 590 138 122 107
71
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 2396 2601 2892 3072 4107 5263 1790
Size: 606 205 291 180 1035 1156 2573
Fragments arranged by size:
2573 1156 1035 606 291 205 180
BmgBI CAC'GTC
Cuts at: 1318 2086 2101 2199 2260 2770 1318
Size: 768 15 98 61 510 4594
Fragments arranged by size:
4594 768 510 98 61 15
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2020 2151 3120
Size: 416 53 83 399 24 603 131 969
Cuts at: 3120 3777 4735 442
Size: 657 958 1753
Fragments arranged by size:
1753 969 958 657 603 416 399 131
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4706 1239
Size: 3467 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4171 5036 5334 4171
Size: 865 298 4883
Fragments arranged by size:
4883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4709 882
Size: 617 3210 2219
Fragments arranged by size:
3210 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2785 3067 3837 493
Size: 711 1581 282 770 2702
Fragments arranged by size:
2702 1581 770 711 282
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 2139 2207 2412
Size: 53 83 186 936 483 123 68 205
Cuts at: 2412 2631 2637 3853 4235 4870 275
Size: 219 6 1216 382 635 1451
Fragments arranged by size:
1451 1216 936 635 483 382 219 205
186 123 83 68 53 6
BsaWI w'CCGG_w
Cuts at: 2527 4479 4877 5151 5298 5709 2527
Size: 1952 398 274 147 411 2864
Fragments arranged by size:
2864 1952 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2279 3146 3176 3197
Size: 30 165 30 846 30 867 30 21
Cuts at: 3197 3227 1178
Size: 30 3997
Fragments arranged by size:
3997 867 846 165 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 2840 3154 3268 3271 1186
Size: 1231 423 314 114 3 3961
Fragments arranged by size:
3961 1231 423 314 114 3
BseYI C'CCAG_C
Cuts at: 2032 5249 5745 2032
Size: 3217 496 2333
Fragments arranged by size:
3217 2333 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2570 1163
Size: 573 834 4639
Fragments arranged by size:
4639 834 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2181 2379 2528 3049 3192
Size: 275 186 39 141 198 149 521 143
Cuts at: 3192 3496 4257 5285 1540
Size: 304 761 1028 2301
Fragments arranged by size:
2301 1028 761 521 304 275 198 186
149 143 141 39
BsiHKAI G_wGCw'C
Cuts at: 2568 2679 2784 2892 3072 4107 4192 5263 2568
Size: 111 105 108 180 1035 85 1071 3351
Fragments arranged by size:
3351 1071 1035 180 111 108 105 85
BsmI GAATG_Cn'
Cuts at: 3557 3650 3557
Size: 93 5953
BsmBI CGTCTCn'nnnn_
Cuts at: 2091 2091
Size: 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 2568 2601 2679 2700 2784 2892
Size: 399 606 172 33 78 21 84 108
Cuts at: 2892 3072 3176 3365 4107 4192 5263 1391
Size: 180 104 189 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 399 189 180 172
108 104 85 84 78 33 21
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 3935 5665 3935
Size: 1730 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3401 844
Size: 2557 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3933 1394
Size: 429 2110 3507
Fragments arranged by size:
3507 2110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 4715 65
Size: 1183 3293 174 1396
Fragments arranged by size:
3293 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 2180 2456 2633 3024 3187 3302
Size: 110 208 162 276 177 391 163 115
Cuts at: 3302 4690 4877 1700
Size: 1388 187 2869
Fragments arranged by size:
2869 1388 391 276 208 187 177 163
162 115 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6046
BssHII G'CGCG_C
Cuts at: 1925 2945 1925
Size: 1020 5026
BssSI C'ACGA_G
Cuts at: 2165 2444 2753 3073 4106 5118 2165
Size: 279 309 320 1033 1012 3093
Fragments arranged by size:
3093 1033 1012 320 309 279
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3102 794
Size: 2308 3738
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 2545 1184
Size: 1361 4685
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6046
BstYI r'GATC_y
Cuts at: 1972 2964 3376 3743 4128 4145 5597 5683 5695
Size: 992 412 367 385 17 1452 86 12
Cuts at: 5695 6041 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 992 412 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2076 2415 2775 513
Size: 552 1011 339 360 3784
Fragments arranged by size:
3784 1011 552 360 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5307
Fragments arranged by size:
5307 683 56
BtsI GCAGTG_nn'
Cuts at: 3562 4356 4376 3562
Size: 794 20 5232
Fragments arranged by size:
5232 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 2961 3350 2961
Size: 389 5657
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2178 2182
Size: 54 1425 50 275 18 258 90 4
Cuts at: 2182 2376 2401 2458 2539 2616 2709 3189 3304
Size: 194 25 57 81 77 93 480 115
Cuts at: 3304 3493 4382 6011 8
Size: 189 889 1629 43
Fragments arranged by size:
1629 1425 889 480 275 258 194 189
115 93 90 81 77 57 54 50
43 25 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2178 2376 3189 3493 1537
Size: 275 366 198 813 304 4090
Fragments arranged by size:
4090 813 366 304 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2435 3325 3976 1353
Size: 1082 890 651 3423
Fragments arranged by size:
3423 1082 890 651
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4633 5007 5153 387
Size: 1064 138 3044 374 146 1280
Fragments arranged by size:
3044 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2677 2677
Size: 6046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4109 4706 5493 5761 1239
Size: 393 2477 597 787 268 1524
Fragments arranged by size:
2477 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2392 2392
Size: 6046
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FseI GG_CCGG'CC
Cuts at: 2184 2184
Size: 6046
FspI TGC'GCA
Cuts at: 3137 4552 3137
Size: 1415 4631
HaeII r_GCGC'y
Cuts at: 1678 2142 2210 2634 2640 3031 5193 1678
Size: 464 68 424 6 391 2162 2531
Fragments arranged by size:
2531 2162 464 424 391 68 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4687 4719 4761 4793 1373
Size: 32 135 32 3115 32 42 32 2626
Fragments arranged by size:
3115 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2482 2875 3390 3636 669
Size: 988 281 544 393 515 246 3079
Fragments arranged by size:
3079 988 544 515 393 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 2875 3636 2875
Size: 761 5285
KasI G'GCGC_C
Cuts at: 2138 2206 2630 2636 2138
Size: 68 424 6 5548
Fragments arranged by size:
5548 424 68 6
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 2463 5922 2463
Size: 3459 2587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5160 5344 1305
Size: 3855 184 2007
Fragments arranged by size:
3855 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2711 10
Size: 54 1425 343 879 3345
Fragments arranged by size:
3345 1425 879 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2606 2787 2885 3065 3230 4004 4363
Size: 648 1440 181 98 180 165 774 359
Cuts at: 4363 4522 518
Size: 159 2042
Fragments arranged by size:
2042 1440 774 648 359 181 180 165
159 98
MspA1I CmG'CkG
Cuts at: 1606 2078 2417 2584 2707 3462 4139 5287 5532
Size: 472 339 167 123 755 677 1148 245
Cuts at: 5532 1606
Size: 2120
Fragments arranged by size:
2120 1148 755 677 472 339 245 167
123
NaeI GCC'GGC
Cuts at: 2020 2182 2458 2635 3189 3304 2020
Size: 162 276 177 554 115 4762
Fragments arranged by size:
4762 554 276 177 162 115
NarI GG'CG_CC
Cuts at: 2139 2207 2631 2637 2139
Size: 68 424 6 5548
Fragments arranged by size:
5548 424 68 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5494
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NgoMIV G'CCGG_C
Cuts at: 2018 2180 2456 2633 3187 3302 2018
Size: 162 276 177 554 115 4762
Fragments arranged by size:
4762 554 276 177 162 115
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 2518 2656 3064 3404 4949 1132
Size: 97 1289 138 408 340 1545 2229
Fragments arranged by size:
2229 1545 1289 408 340 138 97
PasI CC'CwG_GG
Cuts at: 1348 2233 1348
Size: 885 5161
PciI A'CATG_T
Cuts at: 2514 4945 2514
Size: 2431 3615
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6046
PfoI T'CCnGG_A
Cuts at: 2010 2010
Size: 6046
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PmlI CAC'GTG
Cuts at: 3067 3067
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PspOMI G'GGCC_C
Cuts at: 2392 2597 2392
Size: 205 5841
PstI C_TGCA'G
Cuts at: 830 3398 830
Size: 2568 3478
PvuI CG_AT'CG
Cuts at: 2001 2040 2001
Size: 39 6007
PvuII CAG'CTG
Cuts at: 2584 2707 2584
Size: 123 5923
SacI G_AGCT'C
Cuts at: 2679 2679
Size: 6046
SacII CC_GC'GG
Cuts at: 2079 2418 2079
Size: 339 5707
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SfcI C'TryA_G
Cuts at: 826 1045 3394 4529 5210 5401 826
Size: 219 2349 1135 681 191 1471
Fragments arranged by size:
2349 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2400 2400
Size: 6046
SfoI GGC'GCC
Cuts at: 2140 2208 2632 2638 2140
Size: 68 424 6 5548
Fragments arranged by size:
5548 424 68 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 2633 1700
Size: 933 5113
SmaI CCC'GGG
Cuts at: 1872 2253 2437 3373 1872
Size: 381 184 936 4545
Fragments arranged by size:
4545 936 381 184
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4150 5051 5313 820
Size: 197 926 2207 901 262 1553
Fragments arranged by size:
2207 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6046
StyI C'CwwG_G
Cuts at: 513 1065 3485 513
Size: 552 2420 3074
Fragments arranged by size:
3074 2420 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4073 4090 4243 4428 4873 325
Size: 1255 2493 17 153 185 445 1498
Fragments arranged by size:
2493 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4292 6004 96
Size: 274 80 33 51 1443 2315 1712 138
Fragments arranged by size:
2315 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2249 2281 3146 3178 6006 6038 2249
Size: 32 865 32 2828 32 2257
Fragments arranged by size:
2828 2257 865 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2866 2866
Size: 6046
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 2251 2435 3371 1870
Size: 381 184 936 4545
Fragments arranged by size:
4545 936 381 184
XmnI GAAnn'nnTTC
Cuts at: 2996 4175 2996
Size: 1179 4867
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII
SanDI SapI SexAI SrfI SwaI