Restriction map for
pFN21AB7754
(Circular) (Six-base) MAPSORT of: pFN21AB7754.seq Check: 3317 from: 1 to: 6193
pFN21AB7754 6193 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3252 4003 278
Size: 53 83 186 936 1716 751 2468
Fragments arranged by size:
2468 1716 936 751 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2128 2469 2699 3389 3418 4256 5640 5908
Size: 496 341 230 690 29 838 1384 268
Cuts at: 5908 1632
Size: 1917
Fragments arranged by size:
1917 1384 838 690 496 341 268 230
29
AfeI AGC'GCT
Cuts at: 3179 3179
Size: 6193
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2484 3081 3101 3240 3242 5092 6069 1203
Size: 1281 597 20 139 2 1850 977 1327
Fragments arranged by size:
1850 1327 1281 977 597 139 20 2
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 2641 4922 2641
Size: 2281 3912
AleI CACnn'nnGTG
Cuts at: 1166 2957 1166
Size: 1791 4402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 2188 2254 2560 3017 3936 5508 2188
Size: 66 306 457 919 1572 2873
Fragments arranged by size:
2873 1572 919 457 306 66
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 2995 3502 3728 3880 5989 1493
Size: 1502 507 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 507 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2977 3518 1870
Size: 73 1034 541 4545
Fragments arranged by size:
4545 1034 541 73
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3514 4969 618
Size: 325 217 627 1727 1455 1842
Fragments arranged by size:
1842 1727 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2304 3026 3512 1391
Size: 913 722 486 4072
Fragments arranged by size:
4072 913 722 486
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2380 928
Size: 971 481 4741
Fragments arranged by size:
4741 971 481
BbvCI CC'TCA_GC
Cuts at: 2222 2724 2222
Size: 502 5691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4804 136
Size: 107 122 71 631 605 3132 1525
Fragments arranged by size:
3132 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2746 6188 2746
Size: 3442 2751
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 4254 5410 1790
Size: 2464 1156 2573
Fragments arranged by size:
2573 2464 1156
BmgBI CAC'GTC
Cuts at: 1318 2116 2485 3082 1318
Size: 798 369 597 4429
Fragments arranged by size:
4429 798 597 369
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3020 3924 4882
Size: 416 53 83 399 24 1603 904 958
Cuts at: 4882 442
Size: 1753
Fragments arranged by size:
1753 1603 958 904 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2369 2443 4853 1239
Size: 1049 81 74 2410 2579
Fragments arranged by size:
2579 2410 1049 81 74
Bpu10I CC'TnA_GC
Cuts at: 2222 2724 2222
Size: 502 5691
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2925 4318 5183 5481 2925
Size: 1393 865 298 3637
Fragments arranged by size:
3637 1393 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2644 4856 882
Size: 617 1145 2212 2219
Fragments arranged by size:
2219 2212 1145 617
BsaAI yAC'GTr
Cuts at: 493 1204 2137 3102 3243 3984 493
Size: 711 933 965 141 741 2702
Fragments arranged by size:
2702 965 933 741 711 141
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3249 3457 4000 4382
Size: 53 83 186 936 1716 208 543 382
Cuts at: 4382 5017 275
Size: 635 1451
Fragments arranged by size:
1716 1451 936 635 543 382 208 186
83 53
BsaWI w'CCGG_w
Cuts at: 3392 4626 5024 5298 5445 5856 3392
Size: 1234 398 274 147 411 3729
Fragments arranged by size:
3729 1234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5968
Fragments arranged by size:
5968 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2494 2883 2908 1186
Size: 1308 389 25 4471
Fragments arranged by size:
4471 1308 389 25
BseYI C'CCAG_C
Cuts at: 2987 5396 5892 2987
Size: 2409 496 3288
Fragments arranged by size:
3288 2409 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 2444 1163
Size: 573 615 93 4912
Fragments arranged by size:
4912 615 573 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 3378 4254 4339 5410 2304
Size: 1074 876 85 1071 3087
Fragments arranged by size:
3087 1074 1071 876 85
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
BsmBI CGTCTCn'nnnn_
Cuts at: 2865 2865
Size: 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 3026 3378 3512 4254 4339 5410
Size: 399 514 722 352 134 742 85 1071
Cuts at: 5410 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 722 514 399 352 134
85
BspEI T'CCGG_A
Cuts at: 3392 5856 3392
Size: 2464 3729
BspHI T'CATG_A
Cuts at: 2280 2316 4082 5812 2280
Size: 36 1766 1730 2661
Fragments arranged by size:
2661 1766 1730 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 3548 844
Size: 1520 1184 3489
Fragments arranged by size:
3489 1520 1184
BsrBI CCG'CTC
Cuts at: 1394 1823 4080 1394
Size: 429 2257 3507
Fragments arranged by size:
3507 2257 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4837 5024 1700
Size: 110 3027 187 2869
Fragments arranged by size:
3027 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2560 794
Size: 1766 4427
BstBI TT'CG_AA
Cuts at: 3324 4997 3324
Size: 1673 4520
BstEII G'GTnAC_C
Cuts at: 1184 2395 2633 1184
Size: 1211 238 4744
Fragments arranged by size:
4744 1211 238
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 2959 2749
Size: 210 5983
BstYI r'GATC_y
Cuts at: 1972 2250 2556 2674 2746 3523 3890 4275 4292
Size: 278 306 118 72 777 367 385 17
Cuts at: 4292 5744 5830 5842 6188 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 777 385 367 346 306 278
118 86 72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2072 2404 513
Size: 552 939 68 332 4302
Fragments arranged by size:
4302 939 552 332 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 3059 3709 4503 4523 3059
Size: 650 794 20 4729
Fragments arranged by size:
4729 794 650 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DraIII CAC_nnn'GTG
Cuts at: 2116 2116
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3043 3640 4529
Size: 54 1425 50 275 18 1213 597 889
Cuts at: 4529 6158 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1213 889 597 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 2928 3086 4123 1353
Size: 1335 240 158 1037 3423
Fragments arranged by size:
3423 1335 1037 240 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2302 2302
Size: 6193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 2288 2369 2443 2469 2699 3389
Size: 393 496 160 81 74 26 230 690
Cuts at: 3389 3418 4256 4853 5640 5908 1239
Size: 29 838 597 787 268 1524
Fragments arranged by size:
1524 838 787 690 597 496 393 268
230 160 81 74 29 26
EcoO109I rG'GnC_Cy
Cuts at: 2353 2569 2773 3014 3234 2353
Size: 216 204 241 220 5312
Fragments arranged by size:
5312 241 220 216 204
EcoRI G'AATT_C
Cuts at: 2995 3502 3880 2995
Size: 507 378 5308
Fragments arranged by size:
5308 507 378
EcoRV GAT'ATC
Cuts at: 2437 2437
Size: 6193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2680 3298 2680
Size: 618 5575
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 3181 5340 1678
Size: 1503 2159 2531
Fragments arranged by size:
2531 2159 1503
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 2903 2935 4834
Size: 32 135 32 1055 32 244 32 1899
Cuts at: 4834 4866 4908 4940 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1899 1055 244 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3537 3783 669
Size: 988 281 1599 246 3079
Fragments arranged by size:
3079 1599 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2844 748
Size: 2096 4097
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2043 2151 5307 5491 1305
Size: 738 108 3156 184 2007
Fragments arranged by size:
3156 2007 738 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3045 10
Size: 54 1425 343 1213 3158
Fragments arranged by size:
3158 1425 1213 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2026 2264 2957 3039 4151 4510 4669
Size: 648 860 238 693 82 1112 359 159
Cuts at: 4669 518
Size: 2042
Fragments arranged by size:
2042 1112 860 693 648 359 238 159
82
MspA1I CmG'CkG
Cuts at: 1606 2157 2859 3609 4286 5434 5679 1606
Size: 551 702 750 677 1148 245 2120
Fragments arranged by size:
2120 1148 750 702 677 551 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4702
Fragments arranged by size:
4702 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 2263 3078 3551 5096 1132
Size: 97 1034 815 473 1545 2229
Fragments arranged by size:
2229 1545 1034 815 473 97
PasI CC'CwG_GG
Cuts at: 1348 2565 1348
Size: 1217 4976
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2385 1108
Size: 1277 4916
PfoI T'CCnGG_A
Cuts at: 2253 2582 2253
Size: 329 5864
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PmlI CAC'GTG
Cuts at: 2137 3243 2137
Size: 1106 5087
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2353 2569 2773 2353
Size: 216 204 5773
Fragments arranged by size:
5773 216 204
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PstI C_TGCA'G
Cuts at: 830 2556 3545 830
Size: 1726 989 3478
Fragments arranged by size:
3478 1726 989
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 2157 2157
Size: 6193
SacI G_AGCT'C
Cuts at: 2304 2304
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SanDI GG'GwC_CC
Cuts at: 2569 2569
Size: 6193
SapI GCTCTTCn'nnn_
Cuts at: 2928 2928
Size: 6193
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2552 3355 3541 4676 5357 5548 826
Size: 219 1507 803 186 1135 681 191 1471
Fragments arranged by size:
1507 1471 1135 803 681 219 191 186
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 2979 3520 1872
Size: 1107 541 4545
Fragments arranged by size:
4545 1107 541
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2940 4297 5198 5460 820
Size: 197 926 997 1357 901 262 1553
Fragments arranged by size:
1553 1357 997 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2004 2664 3054 3632 513
Size: 552 939 660 390 578 3074
Fragments arranged by size:
3074 939 660 578 552 390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3284 4220 4237 4390 4575 5020 325
Size: 1255 1704 936 17 153 185 445 1498
Fragments arranged by size:
1704 1498 1255 936 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4439 6151 96
Size: 274 80 33 51 1443 2462 1712 138
Fragments arranged by size:
2462 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2662 2662
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 2977 3518 1870
Size: 1107 541 4545
Fragments arranged by size:
4545 1107 541
XmnI GAAnn'nnTTC
Cuts at: 3179 3263 3320 3347 4322 3179
Size: 84 57 27 975 5050
Fragments arranged by size:
5050 975 84 57 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3250 4001 276
Size: 53 83 186 936 1716 751 2468
Fragments arranged by size:
2468 1716 936 751 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI