Restriction map for
pFN21AB7752
(Circular) (Six-base) MAPSORT of: pFN21AB7752.seq Check: 2873 from: 1 to: 6175
pFN21AB7752 6175 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3518 1937
Size: 1581 4594
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 4302 4675 1563
Size: 2739 373 3063
Fragments arranged by size:
3063 2739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2511 2632 2777 3281 4238 5622 5890 1632
Size: 879 121 145 504 957 1384 268 1917
Fragments arranged by size:
1917 1384 957 879 504 268 145 121
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5978
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 2687 2948 1166
Size: 1521 261 4393
Fragments arranged by size:
4393 1521 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6175
AlwNI CAG_nnn'CTG
Cuts at: 2292 3918 5490 2292
Size: 1626 1572 2977
Fragments arranged by size:
2977 1626 1572
ApaLI G'TGCA_C
Cuts at: 4232 5388 4232
Size: 1156 5019
ApoI r'AATT_y
Cuts at: 1493 2573 3357 3484 3710 3862 5971 1493
Size: 1080 784 127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 784 226 152 127
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 2992 3369 3412 3500 1870
Size: 73 1049 377 43 88 4545
Fragments arranged by size:
4545 1049 377 88 73 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2188 2221 2188
Size: 33 6142
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3496 4951 618
Size: 325 217 627 1709 1455 1842
Fragments arranged by size:
1842 1709 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2484 3311 3320 3419 3494 1391
Size: 1093 827 9 99 75 4072
Fragments arranged by size:
4072 1093 827 99 75 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2745 3393 928
Size: 971 846 648 3710
Fragments arranged by size:
3710 971 846 648
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 5277 4067
Size: 1210 4965
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6175
BfrBI ATG'CAT
Cuts at: 3220 3220
Size: 6175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2522 3171 4786
Size: 107 122 71 631 605 850 649 1615
Cuts at: 4786 136
Size: 1525
Fragments arranged by size:
1615 1525 850 649 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6170 6170
Size: 6175
BlpI GC'TnA_GC
Cuts at: 3592 3592
Size: 6175
Bme1580I G_kGCm'C
Cuts at: 1790 4236 5392 1790
Size: 2446 1156 2573
Fragments arranged by size:
2573 2446 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2918 3191 3906
Size: 416 53 83 399 24 1501 273 715
Cuts at: 3906 4864 442
Size: 958 1753
Fragments arranged by size:
1753 1501 958 715 416 399 273 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3424 3424
Size: 6175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3356 4835 1239
Size: 2117 1479 2579
Fragments arranged by size:
2579 2117 1479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3272 4300 5165 5463 2849
Size: 423 1028 865 298 3561
Fragments arranged by size:
3561 1028 865 423 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4838 882
Size: 617 3339 2219
Fragments arranged by size:
3339 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3966 493
Size: 711 2762 2702
Fragments arranged by size:
2762 2702 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2895 3186 3982 4364
Size: 53 83 186 936 1362 291 796 382
Cuts at: 4364 4999 275
Size: 635 1451
Fragments arranged by size:
1451 1362 936 796 635 382 291 186
83 53
BsaWI w'CCGG_w
Cuts at: 3433 4608 5006 5280 5427 5838 3433
Size: 1175 398 274 147 411 3770
Fragments arranged by size:
3770 1175 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2021 2051 2616 2646 2765
Size: 30 165 30 618 30 565 30 119
Cuts at: 2765 2795 1178
Size: 30 4558
Fragments arranged by size:
4558 618 565 165 119 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2462 3409 3440 1186
Size: 877 399 947 31 3921
Fragments arranged by size:
3921 947 877 399 31
BseYI C'CCAG_C
Cuts at: 5378 5874 5378
Size: 496 5679
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3146 1163
Size: 573 1410 4192
Fragments arranged by size:
4192 1410 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3170 3625 4386 5414 1540
Size: 275 186 1169 455 761 1028 2301
Fragments arranged by size:
2301 1169 1028 761 455 275 186
BsiHKAI G_wGCw'C
Cuts at: 3057 3419 4236 4321 5392 3057
Size: 362 817 85 1071 3840
Fragments arranged by size:
3840 1071 817 362 85
BsmI GAATG_Cn'
Cuts at: 3222 3686 3779 3222
Size: 464 93 5618
Fragments arranged by size:
5618 464 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3335 3335
Size: 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2484 3057 3311 3320 3419 3494 4236
Size: 399 694 573 254 9 99 75 742
Cuts at: 4236 4321 5392 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 694 573 399 254 99
85 75 9
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3048 3229 3530 844
Size: 2204 181 301 3489
Fragments arranged by size:
3489 2204 301 181
BsrBI CCG'CTC
Cuts at: 1394 1823 2434 2991 3374 4062 1394
Size: 429 611 557 383 688 3507
Fragments arranged by size:
3507 688 611 557 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 2511 3466 4670 4844 65
Size: 1183 1263 955 1204 174 1396
Fragments arranged by size:
1396 1263 1204 1183 955 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3165 3289 4819 5006 1700
Size: 110 1355 124 1530 187 2869
Fragments arranged by size:
2869 1530 1355 187 124 110
BsrGI T'GTAC_A
Cuts at: 96 3141 96
Size: 3045 3130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 4235 5247 4235
Size: 1012 5163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2322 2968 3461 794
Size: 1528 646 493 3508
Fragments arranged by size:
3508 1528 646 493
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 2064 1184
Size: 880 5295
BstXI CCAn_nnnn'nTGG
Cuts at: 2374 2374
Size: 6175
BstYI r'GATC_y
Cuts at: 1972 2500 3505 3872 4257 4274 5726 5812 5824
Size: 528 1005 367 385 17 1452 86 12
Cuts at: 5824 6170 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1005 528 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 2367 513
Size: 552 939 351 12 4321
Fragments arranged by size:
4321 939 552 351 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2335 2513 487
Size: 683 56 1109 178 4149
Fragments arranged by size:
4149 1109 683 178 56
BtsI GCAGTG_nn'
Cuts at: 2165 2462 3403 3691 4485 4505 2165
Size: 297 941 288 794 20 3835
Fragments arranged by size:
3835 941 794 297 288 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2007 2042 529
Size: 35 1443 35 4662
Fragments arranged by size:
4662 1443 35 35
DraI TTT'AAA
Cuts at: 3479 3479
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2364 2400
Size: 54 1425 50 275 18 265 269 36
Cuts at: 2400 2593 2676 3167 3622 4511 6140 8
Size: 193 83 491 455 889 1629 43
Fragments arranged by size:
1629 1425 889 491 455 275 269 265
193 83 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3167 3622 1537
Size: 275 1355 455 4090
Fragments arranged by size:
4090 1355 455 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2487 3017 3308 4105 1353
Size: 1134 530 291 797 3423
Fragments arranged by size:
3423 1134 797 530 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2455 2584 3419 4762 5136 5282
Size: 1064 138 866 129 835 1343 374 146
Cuts at: 5282 387
Size: 1280
Fragments arranged by size:
1343 1280 1064 866 835 374 146 138
129
EcoICRI GAG'CTC
Cuts at: 3417 3417
Size: 6175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2511 2632 2777 3281 3356 4238 4835
Size: 393 879 121 145 504 75 882 597
Cuts at: 4835 5622 5890 1239
Size: 787 268 1524
Fragments arranged by size:
1524 882 879 787 597 504 393 268
145 121 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2756 2756
Size: 6175
EcoO109I rG'GnC_Cy
Cuts at: 3448 3448
Size: 6175
EcoRI G'AATT_C
Cuts at: 3357 3484 3862 3357
Size: 127 378 5670
Fragments arranged by size:
5670 378 127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 6175
FseI GG_CCGG'CC
Cuts at: 3169 3169
Size: 6175
FspI TGC'GCA
Cuts at: 4681 4681
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 5322 1678
Size: 3644 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2765 2797 4816
Size: 32 135 32 449 32 712 32 2019
Cuts at: 4816 4848 4890 4922 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2019 712 449 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3064 3519 3765 669
Size: 988 281 1044 82 455 246 3079
Fragments arranged by size:
3079 1044 988 455 281 246 82
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6175
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2916 5289 5473 1305
Size: 1611 2373 184 2007
Fragments arranged by size:
2373 2007 1611 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 2402 2595 10
Size: 54 1425 343 534 36 193 3590
Fragments arranged by size:
3590 1425 534 343 193 54 36
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2366 2687 2948 4133 4492 4651
Size: 648 1188 12 321 261 1185 359 159
Cuts at: 4651 518
Size: 2042
Fragments arranged by size:
2042 1188 1185 648 359 321 261 159
12
MspA1I CmG'CkG
Cuts at: 1606 2202 2289 2329 3155 3591 4268 5416 5661
Size: 596 87 40 826 436 677 1148 245
Cuts at: 5661 1606
Size: 2120
Fragments arranged by size:
2120 1148 826 677 596 436 245 87
40
NaeI GCC'GGC
Cuts at: 3167 3167
Size: 6175
NcoI C'CATG_G
Cuts at: 513 1065 2004 2367 513
Size: 552 939 363 4321
Fragments arranged by size:
4321 939 552 363
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NgoMIV G'CCGG_C
Cuts at: 3165 3165
Size: 6175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NsiI A_TGCA'T
Cuts at: 3222 3222
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 2122 3224 3533 5078 1132
Size: 97 893 1102 309 1545 2229
Fragments arranged by size:
2229 1545 1102 893 309 97
PasI CC'CwG_GG
Cuts at: 1348 2545 1348
Size: 1197 4978
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6175
PfoI T'CCnGG_A
Cuts at: 3333 3333
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 5757 5789 4244
Size: 32 1481 32 4630
Fragments arranged by size:
4630 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3448 3448
Size: 6175
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6175
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PstI C_TGCA'G
Cuts at: 830 2469 2964 3527 830
Size: 1639 495 563 3478
Fragments arranged by size:
3478 1639 563 495
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 2289 2329 2289
Size: 40 6135
SacI G_AGCT'C
Cuts at: 3419 3419
Size: 6175
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SapI GCTCTTCn'nnn_
Cuts at: 3308 3308
Size: 6175
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2465 2960 3523 4658 5339 5530 826
Size: 219 1420 495 563 1135 681 191 1471
Fragments arranged by size:
1471 1420 1135 681 563 495 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6175
SmaI CCC'GGG
Cuts at: 1872 3502 1872
Size: 1630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2864 3251 4279 5180 5442 820
Size: 197 926 921 387 1028 901 262 1553
Fragments arranged by size:
1553 1028 926 921 901 387 262 197
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3533 1132
Size: 2401 3774
SspI AAT'ATT
Cuts at: 5 52 4099 5
Size: 47 4047 2081
Fragments arranged by size:
4047 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6175
StyI C'CwwG_G
Cuts at: 513 1065 2004 2367 3283 3614 513
Size: 552 939 363 916 331 3074
Fragments arranged by size:
3074 939 916 552 363 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2617 4202 4219 4372 4557 5002 325
Size: 1255 1037 1585 17 153 185 445 1498
Fragments arranged by size:
1585 1498 1255 1037 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3141 4421 6133
Size: 274 80 33 51 1443 1164 1280 1712
Cuts at: 6133 96
Size: 138
Fragments arranged by size:
1712 1443 1280 1164 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2616 2648 3171 3203 6135 6167 2616
Size: 32 523 32 2932 32 2624
Fragments arranged by size:
2932 2624 523 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6175
XbaI T'CTAG_A
Cuts at: 2380 3511 2380
Size: 1131 5044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2686 2905 2686
Size: 219 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 3500 1870
Size: 1630 4545
XmnI GAAnn'nnTTC
Cuts at: 4304 4304
Size: 6175
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FspAI KasI
NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI