Restriction map for
pFN21AB7751
(Circular) (Six-base) MAPSORT of: pFN21AB7751.seq Check: 1035 from: 1 to: 6040
pFN21AB7751 6040 bp
With 182 enzymes: SgfI *
March 28, 2012 13:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 2502 4167 4540 1563
Size: 939 1665 373 3063
Fragments arranged by size:
3063 1665 939 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2206 2466 3062 4103 5487 5755 1632
Size: 574 260 596 1041 1384 268 1917
Fragments arranged by size:
1917 1384 1041 596 574 268 260
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 4939 5916 1203
Size: 3736 977 1327
Fragments arranged by size:
3736 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 3783 5355 3783
Size: 1572 4468
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2958 3365 1870
Size: 73 1015 407 4545
Fragments arranged by size:
4545 1015 407 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3361 4816 618
Size: 325 217 627 1574 1455 1842
Fragments arranged by size:
1842 1574 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2638 3359 1391
Size: 1247 721 4072
Fragments arranged by size:
4072 1247 721
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5069
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 2614 1548
Size: 1066 4974
BfrBI ATG'CAT
Cuts at: 3136 3136
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4651 136
Size: 107 122 71 631 605 2979 1525
Fragments arranged by size:
2979 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2463 3216 6035 2463
Size: 753 2819 2468
Fragments arranged by size:
2819 2468 753
BlpI GC'TnA_GC
Cuts at: 2873 3457 2873
Size: 584 5456
Bme1580I G_kGCm'C
Cuts at: 1790 4101 5257 1790
Size: 2311 1156 2573
Fragments arranged by size:
2573 2311 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3771 4729 442
Size: 416 53 83 399 24 2354 958 1753
Fragments arranged by size:
2354 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4700 1239
Size: 3461 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2458 3135 4165 5030 5328 2458
Size: 677 1030 865 298 3170
Fragments arranged by size:
3170 1030 865 677 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2460 4703 882
Size: 617 961 2243 2219
Fragments arranged by size:
2243 2219 961 617
BsaAI yAC'GTr
Cuts at: 493 1204 3012 3831 493
Size: 711 1808 819 2702
Fragments arranged by size:
2702 1808 819 711
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3847 4229 4864 275
Size: 53 83 186 936 2314 382 635 1451
Fragments arranged by size:
2314 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4473 4871 5145 5292 5703 4473
Size: 398 274 147 411 4810
Fragments arranged by size:
4810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 1178
Size: 30 165 30 1002 30 4783
Fragments arranged by size:
4783 1002 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6040
BseYI C'CCAG_C
Cuts at: 5243 5739 5243
Size: 496 5544
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2933 1163
Size: 573 1197 4270
Fragments arranged by size:
4270 1197 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2638 2652 4101 4186 5257 2638
Size: 14 1449 85 1071 3421
Fragments arranged by size:
3421 1449 1071 85 14
BsmI GAATG_Cn'
Cuts at: 2232 3551 3644 2232
Size: 1319 93 4628
Fragments arranged by size:
4628 1319 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2638 2652 3359 4101 4186 5257 1391
Size: 399 848 14 707 742 85 1071 2174
Fragments arranged by size:
2174 1071 848 742 707 399 85 14
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2250 3395 844
Size: 1406 1145 3489
Fragments arranged by size:
3489 1406 1145
BsrBI CCG'CTC
Cuts at: 1394 1823 2339 3927 1394
Size: 429 516 1588 3507
Fragments arranged by size:
3507 1588 516 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2279 4535 4709 65
Size: 1183 1031 2256 174 1396
Fragments arranged by size:
2256 1396 1183 1031 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2359 96
Size: 2263 3777
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 4100 5112 4100
Size: 1012 5028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2656 794
Size: 1862 4178
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2463 2675 3216 3370 3737 4122 4139 5591
Size: 491 212 541 154 367 385 17 1452
Cuts at: 5591 5677 5689 6035 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 541 491 385 367 346 212
154 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4549
Fragments arranged by size:
4549 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 2996 3556 4350 4370 2996
Size: 560 794 20 4666
Fragments arranged by size:
4666 794 560 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 2835 3344 2835
Size: 509 5531
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2135 5047 809
Size: 507 819 2912 1802
Fragments arranged by size:
2912 1802 819 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2760 3487 4376
Size: 54 1425 50 275 18 930 727 889
Cuts at: 4376 6005 8
Size: 1629 43
Fragments arranged by size:
1629 1425 930 889 727 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3970 1353
Size: 2617 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2636 2636
Size: 6040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2466 3062 4103 4700 5487 5755
Size: 393 574 260 596 1041 597 787 268
Cuts at: 5755 1239
Size: 1524
Fragments arranged by size:
1524 1041 787 597 596 574 393 268
260
EcoO109I rG'GnC_Cy
Cuts at: 3185 3185
Size: 6040
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 6040
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 5187 1678
Size: 3509 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3203 3208 3235 3240 4681
Size: 32 135 32 1631 5 27 5 1441
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1631 1441 135 42 32 32 32
32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2140 2284 3195 3384 3630 669
Size: 988 281 202 144 911 189 246 3079
Fragments arranged by size:
3079 988 911 281 246 202 189 144
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 2841 3335 3639 2841
Size: 494 304 5242
Fragments arranged by size:
5242 494 304
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2762 10
Size: 54 1425 343 930 3288
Fragments arranged by size:
3288 1425 930 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3998 4357 4516 518
Size: 648 843 1989 359 159 2042
Fragments arranged by size:
2042 1989 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2199 2736 3456 4133 5281 5526 1606
Size: 593 537 720 677 1148 245 2120
Fragments arranged by size:
2120 1148 720 677 593 537 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4549
Fragments arranged by size:
4549 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NsiI A_TGCA'T
Cuts at: 3138 3138
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3398 4943 1132
Size: 97 2169 1545 2229
Fragments arranged by size:
2229 2169 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2480 1348
Size: 1132 4908
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3164 1108
Size: 2056 3984
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PmlI CAC'GTG
Cuts at: 3012 3012
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3185 3185
Size: 6040
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PstI C_TGCA'G
Cuts at: 830 3003 3392 830
Size: 2173 389 3478
Fragments arranged by size:
3478 2173 389
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 2199 2736 2199
Size: 537 5503
SacI G_AGCT'C
Cuts at: 2638 2638
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 2999 3388 4523 5204 5395 826
Size: 219 1954 389 1135 681 191 1471
Fragments arranged by size:
1954 1471 1135 681 389 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 2960 3367 1872
Size: 1088 407 4545
Fragments arranged by size:
4545 1088 407
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2473 3150 4144 5045 5307 820
Size: 197 926 530 677 994 901 262 1553
Fragments arranged by size:
1553 994 926 901 677 530 262 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 2004 3479 513
Size: 552 939 1475 3074
Fragments arranged by size:
3074 1475 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2196 2442 4067 4084 4237 4422 4867
Size: 1255 616 246 1625 17 153 185 445
Cuts at: 4867 325
Size: 1498
Fragments arranged by size:
1625 1498 1255 616 445 246 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2359 3273 4286
Size: 274 80 33 51 1443 382 914 1013
Cuts at: 4286 5998 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1013 914 382 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 2773 3376 2773
Size: 603 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2431 3196 2431
Size: 765 5275
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 2958 3365 1870
Size: 1088 407 4545
Fragments arranged by size:
4545 1088 407
XmnI GAAnn'nnTTC
Cuts at: 4169 4169
Size: 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI