Restriction map for
pFN21AB7750
(Circular) (Six-base) MAPSORT of: pFN21AB7750.seq Check: 7114 from: 1 to: 6109
pFN21AB7750 6109 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3452 1937
Size: 1515 4594
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2243 2344 4172 5556 5824 1632
Size: 461 150 101 1828 1384 268 1917
Fragments arranged by size:
1917 1828 1384 461 268 150 101
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 2641 2643 5008 5985 1203
Size: 1438 2 2365 977 1327
Fragments arranged by size:
2365 1438 1327 977 2
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2234 1184
Size: 1050 5059
AlwNI CAG_nnn'CTG
Cuts at: 2272 3852 5424 2272
Size: 1580 1572 2957
Fragments arranged by size:
2957 1580 1572
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 3418 3644 3796 5905 1493
Size: 1925 226 152 2109 1697
Fragments arranged by size:
2109 1925 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 3275 3434 1870
Size: 73 1332 159 4545
Fragments arranged by size:
4545 1332 159 73
BamHI G'GATC_C
Cuts at: 3333 3439 3806 3333
Size: 106 367 5636
Fragments arranged by size:
5636 367 106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3430 4885 618
Size: 325 217 627 1643 1455 1842
Fragments arranged by size:
1842 1643 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2740 3107 3153 3428 1391
Size: 1349 367 46 275 4072
Fragments arranged by size:
4072 1349 367 275 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 928
Size: 971 222 4916
Fragments arranged by size:
4916 971 222
BbvCI CC'TCA_GC
Cuts at: 3019 3019
Size: 6109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 1903
Size: 34 2363 34 3678
Fragments arranged by size:
3678 2363 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3063 4720 136
Size: 107 122 71 631 605 1391 1657 1525
Fragments arranged by size:
1657 1525 1391 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6104 6104
Size: 6109
BlpI GC'TnA_GC
Cuts at: 3526 3526
Size: 6109
Bme1580I G_kGCm'C
Cuts at: 1790 4170 5326 1790
Size: 2380 1156 2573
Fragments arranged by size:
2573 2380 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6109
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2019 2481 2906
Size: 416 53 83 399 24 602 462 425
Cuts at: 2906 3840 4798 442
Size: 934 958 1753
Fragments arranged by size:
1753 958 934 602 462 425 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2759
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 2739 4769 1239
Size: 805 695 2030 2579
Fragments arranged by size:
2579 2030 805 695
Bpu10I CC'TnA_GC
Cuts at: 2044 2786 3019 2044
Size: 742 233 5134
Fragments arranged by size:
5134 742 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4234 5099 5397 4234
Size: 865 298 4946
Fragments arranged by size:
4946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2919 4772 882
Size: 617 1420 1853 2219
Fragments arranged by size:
2219 1853 1420 617
BsaAI yAC'GTr
Cuts at: 493 1204 2644 3900 493
Size: 711 1440 1256 2702
Fragments arranged by size:
2702 1440 1256 711
BsaBI GATnn'nnATC
Cuts at: 6103 6103
Size: 6109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3916 4298 4933 275
Size: 53 83 186 936 2383 382 635 1451
Fragments arranged by size:
2383 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3090 3336 4542 4940 5214 5361 5772 3090
Size: 246 1206 398 274 147 411 3427
Fragments arranged by size:
3427 1206 411 398 274 246 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2533 2563 2766 2796 2987
Size: 30 165 30 1130 30 203 30 191
Cuts at: 2987 3017 1178
Size: 30 4270
Fragments arranged by size:
4270 1130 203 191 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2554 2995 2998 3001 3004 3007 3305 1186
Size: 1368 441 3 3 3 3 298 3990
Fragments arranged by size:
3990 1368 441 298 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3107 3186 3230 5312 5808 3107
Size: 79 44 2082 496 3408
Fragments arranged by size:
3408 2082 496 79 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 3305 1163
Size: 573 450 1119 3967
Fragments arranged by size:
3967 1119 573 450
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2816 3559 4320 5348 1540
Size: 275 186 815 743 761 1028 2301
Fragments arranged by size:
2301 1028 815 761 743 275 186
BsiHKAI G_wGCw'C
Cuts at: 2981 4170 4255 5326 2981
Size: 1189 85 1071 3764
Fragments arranged by size:
3764 1189 1071 85
BsmI GAATG_Cn'
Cuts at: 3620 3713 3620
Size: 93 6016
BsmBI CGTCTCn'nnnn_
Cuts at: 3250 3250
Size: 6109
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2740 2981 3107 3153 3428 4170 4255
Size: 399 950 241 126 46 275 742 85
Cuts at: 4255 5326 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 950 742 399 275 241 126
85 46
BspEI T'CCGG_A
Cuts at: 3090 5772 3090
Size: 2682 3427
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3464 844
Size: 2620 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4604 4778 65
Size: 1183 3356 174 1396
Fragments arranged by size:
3356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4753 4940 1700
Size: 110 2943 187 2869
Fragments arranged by size:
2943 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 2204 3098 4169 5181 2204
Size: 894 1071 1012 3132
Fragments arranged by size:
3132 1071 1012 894
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6109
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstXI CCAn_nnnn'nTGG
Cuts at: 2703 2703
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 2433 3333 3439 3806 4191 4208 5660 5746
Size: 461 900 106 367 385 17 1452 86
Cuts at: 5746 5758 6104 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 900 461 385 367 346 106
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2246 2670 2246
Size: 424 5685
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 513
Size: 552 939 162 4456
Fragments arranged by size:
4456 939 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3297 487
Size: 683 56 2071 3299
Fragments arranged by size:
3299 2071 683 56
BtsI GCAGTG_nn'
Cuts at: 2959 3239 3625 4419 4439 2959
Size: 280 386 794 20 4629
Fragments arranged by size:
4629 794 386 280 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2379 2705 2813
Size: 54 1425 50 275 18 549 326 108
Cuts at: 2813 2847 3556 4445 6074 8
Size: 34 709 889 1629 43
Fragments arranged by size:
1629 1425 889 709 549 326 275 108
54 50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2813 3556 1537
Size: 275 1001 743 4090
Fragments arranged by size:
4090 1001 743 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 2172 2219 3114 4039 1353
Size: 798 21 47 895 925 3423
Fragments arranged by size:
3423 925 895 798 47 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4696 5070 5216 387
Size: 1064 138 3107 374 146 1280
Fragments arranged by size:
3107 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2093 2243 2344 2739 4172 4769
Size: 393 412 49 150 101 395 1433 597
Cuts at: 4769 5556 5824 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1433 787 597 412 395 393 268
150 101 49
EcoO109I rG'GnC_Cy
Cuts at: 2104 2269 2782 3226 3312 2104
Size: 165 513 444 86 4901
Fragments arranged by size:
4901 513 444 165 86
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
EcoRV GAT'ATC
Cuts at: 2422 2422
Size: 6109
FspI TGC'GCA
Cuts at: 4615 4615
Size: 6109
HaeII r_GCGC'y
Cuts at: 1678 2407 3202 5256 1678
Size: 729 795 2054 2531
Fragments arranged by size:
2531 2054 795 729
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2611 2643 2766 2798 2987
Size: 32 135 32 1039 32 123 32 189
Cuts at: 2987 3019 4750 4782 4824 4856 1373
Size: 32 1731 32 42 32 2626
Fragments arranged by size:
2626 1731 1039 189 135 123 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 5985 5985
Size: 6109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2420 2991 5223 5407 1305
Size: 1115 571 2232 184 2007
Fragments arranged by size:
2232 2007 1115 571 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 10
Size: 54 1425 343 549 3738
Fragments arranged by size:
3738 1425 549 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 2837 4067 4426 4585 518
Size: 648 1209 462 1230 359 159 2042
Fragments arranged by size:
2042 1230 1209 648 462 359 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2364 2818 2846 2871 2922 3174 3525
Size: 485 273 454 28 25 51 252 351
Cuts at: 3525 4202 5350 5595 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 485 454 351 273 252
245 51 28 25
NcoI C'CATG_G
Cuts at: 513 1065 2004 2166 513
Size: 552 939 162 4456
Fragments arranged by size:
4456 939 552 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 2813 3556 2813
Size: 743 5366
NspI r_CATG'y
Cuts at: 1132 1229 2314 3467 5012 1132
Size: 97 1085 1153 1545 2229
Fragments arranged by size:
2229 1545 1153 1085 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2272 2903 1108
Size: 1164 631 4314
Fragments arranged by size:
4314 1164 631
PfoI T'CCnGG_A
Cuts at: 2265 2755 2265
Size: 490 5619
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PmlI CAC'GTG
Cuts at: 2644 2644
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2782 3226 3312 2269
Size: 513 444 86 5066
Fragments arranged by size:
5066 513 444 86
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PstI C_TGCA'G
Cuts at: 830 3461 830
Size: 2631 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 2364 2871 2364
Size: 507 5602
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SanDI GG'GwC_CC
Cuts at: 3312 3312
Size: 6109
SapI GCTCTTCn'nnn_
Cuts at: 2172 2219 2172
Size: 47 6062
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 2947 3457 4592 5273 5464 826
Size: 219 1902 510 1135 681 191 1471
Fragments arranged by size:
1902 1471 1135 681 510 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 3436 1872
Size: 1564 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4213 5114 5376 820
Size: 197 926 2270 901 262 1553
Fragments arranged by size:
2270 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6109
StyI C'CwwG_G
Cuts at: 513 1065 2004 2100 2166 2931 3048 3153 3213
Size: 552 939 96 66 765 117 105 60
Cuts at: 3213 3548 513
Size: 335 3074
Fragments arranged by size:
3074 939 765 552 335 117 105 96
66 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 2463 2646 2652 2713 4136 4153
Size: 1255 650 233 183 6 61 1423 17
Cuts at: 4153 4306 4491 4936 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1423 1255 650 445 233 185 183
153 61 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4355 6067 96
Size: 274 80 33 51 1443 2378 1712 138
Fragments arranged by size:
2378 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2677 2709 2875 2907 6069 6101 2677
Size: 32 166 32 3162 32 2685
Fragments arranged by size:
3162 2685 166 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2070 1316
Size: 754 5355
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3160 3160
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6109
XmaI C'CCGG_G
Cuts at: 1870 3434 1870
Size: 1564 4545
XmnI GAAnn'nnTTC
Cuts at: 2445 4238 2445
Size: 1793 4316
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI