Restriction map for
pFN21AB7749
(Circular) (Six-base) MAPSORT of: pFN21AB7749.seq Check: 5035 from: 1 to: 6034
pFN21AB7749 6034 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3844 278
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3377 1937
Size: 1440 4594
Acc65I G'GTAC_C
Cuts at: 2912 3355 2912
Size: 443 5591
AclI AA'CG_TT
Cuts at: 1563 4161 4534 1563
Size: 2598 373 3063
Fragments arranged by size:
3063 2598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4097 5481 5749 1632
Size: 2465 1384 268 1917
Fragments arranged by size:
2465 1917 1384 268
AfeI AGC'GCT
Cuts at: 2987 2987
Size: 6034
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5837
AflIII A'CryG_T
Cuts at: 1203 4933 5910 1203
Size: 3730 977 1327
Fragments arranged by size:
3730 1327 977
AgeI A'CCGG_T
Cuts at: 4865 4865
Size: 6034
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2783 2825 1184
Size: 1599 42 4393
Fragments arranged by size:
4393 1599 42
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2615 2647 2615
Size: 32 6002
AlwNI CAG_nnn'CTG
Cuts at: 3777 5349 3777
Size: 1572 4462
ApaLI G'TGCA_C
Cuts at: 4091 5247 4091
Size: 1156 4878
ApoI r'AATT_y
Cuts at: 1493 3303 3343 3569 3721 5830 1493
Size: 1810 40 226 152 2109 1697
Fragments arranged by size:
2109 1810 1697 226 152 40
AseI AT'TA_AT
Cuts at: 160 1032 4588 160
Size: 872 3556 1606
Fragments arranged by size:
3556 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 3181 3359 1870
Size: 73 1238 178 4545
Fragments arranged by size:
4545 1238 178 73
BamHI G'GATC_C
Cuts at: 3364 3731 3364
Size: 367 5667
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2912 3017 3185 3203
Size: 325 217 627 336 789 105 168 18
Cuts at: 3203 3355 4810 618
Size: 152 1455 1842
Fragments arranged by size:
1842 1455 789 627 336 325 217 168
152 105 18
BanII G_rGCy'C
Cuts at: 1391 2435 2558 2877 3126 3353 1391
Size: 1044 123 319 249 227 4072
Fragments arranged by size:
4072 1044 319 249 227 123
BbeI G_GCGC'C
Cuts at: 2127 3189 2127
Size: 1062 4972
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3091 928
Size: 971 1192 3871
Fragments arranged by size:
3871 1192 971
BbvCI CC'TCA_GC
Cuts at: 2319 2331 2319
Size: 12 6022
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2133 2167 4225 4259 1903
Size: 34 50 34 112 34 2058 34 3678
Fragments arranged by size:
3678 2058 112 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3926 5136 3926
Size: 1210 4824
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6034
BfrBI ATG'CAT
Cuts at: 2845 2845
Size: 6034
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2423 3054 4645
Size: 107 122 71 631 605 751 631 1591
Cuts at: 4645 136
Size: 1525
Fragments arranged by size:
1591 1525 751 631 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2712 6029 2712
Size: 3317 2717
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 2814 3020 4095 5251 1790
Size: 1024 206 1075 1156 2573
Fragments arranged by size:
2573 1156 1075 1024 206
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2872 3765 4723
Size: 416 53 83 399 24 1455 893 958
Cuts at: 4723 442
Size: 1753
Fragments arranged by size:
1753 1455 958 893 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2155 2506 2642 4694 1239
Size: 905 11 351 136 2052 2579
Fragments arranged by size:
2579 2052 905 351 136 11
Bpu10I CC'TnA_GC
Cuts at: 2228 2319 2331 2527 2228
Size: 91 12 196 5735
Fragments arranged by size:
5735 196 91 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4159 5024 5322 4159
Size: 865 298 4871
Fragments arranged by size:
4871 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4697 882
Size: 617 3198 2219
Fragments arranged by size:
3198 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3825 493
Size: 711 2621 2702
Fragments arranged by size:
2702 2621 711
BsaBI GATnn'nnATC
Cuts at: 2711 6028 2711
Size: 3317 2717
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2108 2124 3186 3841
Size: 53 83 186 936 575 16 1062 655
Cuts at: 3841 4223 4858 275
Size: 382 635 1451
Fragments arranged by size:
1451 1062 936 655 635 575 382 186
83 53 16
BsaWI w'CCGG_w
Cuts at: 4467 4865 5139 5286 5697 4467
Size: 398 274 147 411 4804
Fragments arranged by size:
4804 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2615 2645 1178
Size: 30 165 30 1212 30 4567
Fragments arranged by size:
4567 1212 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2376 1186
Size: 1190 4844
BseYI C'CCAG_C
Cuts at: 2954 3214 5237 5733 2954
Size: 260 2023 496 3255
Fragments arranged by size:
3255 2023 496 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 2325 1163
Size: 573 522 67 4872
Fragments arranged by size:
4872 573 522 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2660 3484 4245 5273 1540
Size: 275 186 659 824 761 1028 2301
Fragments arranged by size:
2301 1028 824 761 659 275 186
BsiHKAI G_wGCw'C
Cuts at: 4095 4180 5251 4095
Size: 85 1071 4878
Fragments arranged by size:
4878 1071 85
BsmI GAATG_Cn'
Cuts at: 2398 2845 3545 3638 2398
Size: 447 700 93 4794
Fragments arranged by size:
4794 700 447 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2435 2558 2814 2877 3020 3126 3353
Size: 399 645 123 256 63 143 106 227
Cuts at: 3353 4095 4180 5251 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 645 399 256 227 143
123 106 85 63
BspEI T'CCGG_A
Cuts at: 5697 5697
Size: 6034
BspHI T'CATG_A
Cuts at: 3923 5653 3923
Size: 1730 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3389 844
Size: 2545 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3921 1394
Size: 429 2098 3507
Fragments arranged by size:
3507 2098 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4529 4703 65
Size: 1183 3281 174 1396
Fragments arranged by size:
3281 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2655 2822 3278 4678 4865 1700
Size: 110 845 167 456 1400 187 2869
Fragments arranged by size:
2869 1400 845 456 187 167 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6034
BssHII G'CGCG_C
Cuts at: 1925 2581 2583 2585 2604 1925
Size: 656 2 2 19 5355
Fragments arranged by size:
5355 656 19 2 2
BssSI C'ACGA_G
Cuts at: 2599 4094 5106 2599
Size: 1495 1012 3527
Fragments arranged by size:
3527 1495 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6034
BstBI TT'CG_AA
Cuts at: 4838 4838
Size: 6034
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6034
BstYI r'GATC_y
Cuts at: 1972 2712 3364 3731 4116 4133 5585 5671 5683
Size: 740 652 367 385 17 1452 86 12
Cuts at: 5683 6029 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 740 652 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3189 3232 513
Size: 552 2124 43 3315
Fragments arranged by size:
3315 2124 552 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5295
Fragments arranged by size:
5295 683 56
BtsI GCAGTG_nn'
Cuts at: 3550 4344 4364 3550
Size: 794 20 5220
Fragments arranged by size:
5220 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5999
DraI TTT'AAA
Cuts at: 3338 3338
Size: 6034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5041 809
Size: 507 3725 1802
Fragments arranged by size:
3725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 2457 2657
Size: 54 1425 50 275 18 594 33 200
Cuts at: 2657 2676 3223 3280 3481 4370 5999 8
Size: 19 547 57 201 889 1629 43
Fragments arranged by size:
1629 1425 889 594 547 275 201 200
57 54 50 43 33 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2657 3481 1537
Size: 275 845 824 4090
Fragments arranged by size:
4090 845 824 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2377 3964 1353
Size: 1024 1587 3423
Fragments arranged by size:
3423 1587 1024
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2101 4621 4995 5141 387
Size: 1064 138 512 2520 374 146 1280
Fragments arranged by size:
2520 1280 1064 512 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2155 2506 2642 4097 4694 5481
Size: 393 512 11 351 136 1455 597 787
Cuts at: 5481 5749 1239
Size: 268 1524
Fragments arranged by size:
1524 1455 787 597 512 393 351 268
136 11
EcoO109I rG'GnC_Cy
Cuts at: 3072 3242 3072
Size: 170 5864
EcoRI G'AATT_C
Cuts at: 3303 3343 3721 3303
Size: 40 378 5616
Fragments arranged by size:
5616 378 40
FspI TGC'GCA
Cuts at: 2222 2962 4540 2222
Size: 740 1578 3716
Fragments arranged by size:
3716 1578 740
HaeII r_GCGC'y
Cuts at: 1678 2127 2178 2235 2704 2989 3189 5181 1678
Size: 449 51 57 469 285 200 1992 2531
Fragments arranged by size:
2531 1992 469 449 285 200 57 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2665 2697 2864 2896 4675
Size: 32 135 32 1093 32 167 32 1779
Cuts at: 4675 4707 4749 4781 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1779 1093 167 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2857 3378 3624 669
Size: 988 281 919 521 246 3079
Fragments arranged by size:
3079 988 919 521 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6034
HpaI GTT'AAC
Cuts at: 3624 3624
Size: 6034
KasI G'GCGC_C
Cuts at: 2123 3185 2123
Size: 1062 4972
KpnI G_GTAC'C
Cuts at: 2916 3359 2916
Size: 443 5591
MfeI C'AATT_G
Cuts at: 3633 3633
Size: 6034
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5148 5332 1305
Size: 3843 184 2007
Fragments arranged by size:
3843 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2459 2678 10
Size: 54 1425 343 627 219 3366
Fragments arranged by size:
3366 1425 627 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3042 3992 4351 4510 518
Size: 648 1876 950 359 159 2042
Fragments arranged by size:
2042 1876 950 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2611 2958 3214 3222 3450 4127 5275 5520
Size: 1005 347 256 8 228 677 1148 245
Cuts at: 5520 1606
Size: 2120
Fragments arranged by size:
2120 1148 1005 677 347 256 245 228
8
NaeI GCC'GGC
Cuts at: 2657 2657
Size: 6034
NarI GG'CG_CC
Cuts at: 2124 3186 2124
Size: 1062 4972
NcoI C'CATG_G
Cuts at: 513 1065 3189 513
Size: 552 2124 3358
Fragments arranged by size:
3358 2124 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6034
NgoMIV G'CCGG_C
Cuts at: 2655 2655
Size: 6034
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6034
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NsiI A_TGCA'T
Cuts at: 2847 2847
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 2770 3392 4937 1132
Size: 97 1541 622 1545 2229
Fragments arranged by size:
2229 1545 1541 622 97
PasI CC'CwG_GG
Cuts at: 1348 2908 1348
Size: 1560 4474
PciI A'CATG_T
Cuts at: 4933 4933
Size: 6034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6034
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2615 2647 4103 4135 5616 5648 2615
Size: 32 1456 32 1481 32 3001
Fragments arranged by size:
3001 1481 1456 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3072 3072
Size: 6034
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6034
PsiI TTA'TAA
Cuts at: 2082 3604 2082
Size: 1522 4512
PstI C_TGCA'G
Cuts at: 830 3054 3386 830
Size: 2224 332 3478
Fragments arranged by size:
3478 2224 332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
PvuII CAG'CTG
Cuts at: 2611 2958 2611
Size: 347 5687
SalI G'TCGA_C
Cuts at: 1936 3376 1936
Size: 1440 4594
SanDI GG'GwC_CC
Cuts at: 3072 3072
Size: 6034
SbfI CC_TGCA'GG
Cuts at: 3054 3386 3054
Size: 332 5702
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SexAI A'CCwGG_T
Cuts at: 3330 3330
Size: 6034
SfcI C'TryA_G
Cuts at: 826 1045 3050 3382 4517 5198 5389 826
Size: 219 2005 332 1135 681 191 1471
Fragments arranged by size:
2005 1471 1135 681 332 219 191
SfoI GGC'GCC
Cuts at: 2125 3187 2125
Size: 1062 4972
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 3361 1872
Size: 1489 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4138 5039 5301 820
Size: 197 926 2195 901 262 1553
Fragments arranged by size:
2195 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 2770 3392 1132
Size: 1638 622 3774
Fragments arranged by size:
3774 1638 622
SspI AAT'ATT
Cuts at: 5 52 3958 5
Size: 47 3906 2081
Fragments arranged by size:
3906 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6034
StyI C'CwwG_G
Cuts at: 513 1065 2190 3189 3473 513
Size: 552 1125 999 284 3074
Fragments arranged by size:
3074 1125 999 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2464 3331 3341 4061 4078 4231 4416
Size: 1255 884 867 10 720 17 153 185
Cuts at: 4416 4861 325
Size: 445 1498
Fragments arranged by size:
1498 1255 884 867 720 445 185 153
17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 4280 5992
Size: 274 80 33 51 1443 762 1541 1712
Cuts at: 5992 96
Size: 138
Fragments arranged by size:
1712 1541 1443 762 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5994 6026 5994
Size: 32 6002
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6034
XbaI T'CTAG_A
Cuts at: 3370 3370
Size: 6034
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6034
XmaI C'CCGG_G
Cuts at: 1870 3359 1870
Size: 1489 4545
XmnI GAAnn'nnTTC
Cuts at: 2630 4163 2630
Size: 1533 4501
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3842 276
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SfiI SrfI SwaI
XcmI