Restriction map for
pFN21AB7745
(Circular) (Six-base) MAPSORT of: pFN21AB7745.seq Check: 5365 from: 1 to: 6016
pFN21AB7745 6016 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2195 2195
Size: 6016
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2904 3359 1937
Size: 967 455 4594
Fragments arranged by size:
4594 967 455
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2666 4079 5463 5731 1632
Size: 1034 1413 1384 268 1917
Fragments arranged by size:
1917 1413 1384 1034 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 4915 5892 1203
Size: 3712 977 1327
Fragments arranged by size:
3712 1327 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 2525 1166
Size: 1359 4657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AlwNI CAG_nnn'CTG
Cuts at: 2126 2644 3172 3759 5331 2126
Size: 518 528 587 1572 2811
Fragments arranged by size:
2811 1572 587 528 518
ApaI G_GGCC'C
Cuts at: 2019 2895 2019
Size: 876 5140
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 3325 3551 3703 5812 1493
Size: 1832 226 152 2109 1697
Fragments arranged by size:
2109 1832 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3312 4570 160
Size: 872 2280 1258 1606
Fragments arranged by size:
2280 1606 1258 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2213 2375 3341 1870
Size: 73 76 194 162 966 4545
Fragments arranged by size:
4545 966 194 162 76 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2132 2165 2132
Size: 33 5983
BamHI G'GATC_C
Cuts at: 3346 3713 3346
Size: 367 5649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2161 2176 2183 3337
Size: 325 217 627 272 102 15 7 1154
Cuts at: 3337 4792 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 272 217 102
15 7
BanII G_rGCy'C
Cuts at: 1391 2019 2075 2895 3036 3335 1391
Size: 628 56 820 141 299 4072
Fragments arranged by size:
4072 820 628 299 141 56
BbeI G_GCGC'C
Cuts at: 2165 2180 2165
Size: 15 6001
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2390 2607 928
Size: 971 491 217 4337
Fragments arranged by size:
4337 971 491 217
BbvCI CC'TCA_GC
Cuts at: 2099 2481 2099
Size: 382 5634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2041 2075 2173 2207 4207
Size: 34 50 34 20 34 98 34 2000
Cuts at: 4207 4241 1903
Size: 34 3678
Fragments arranged by size:
3678 2000 98 50 34 34 34 34
34 20
BciVI GTATCCnnnnn_n'
Cuts at: 3908 5118 3908
Size: 1210 4806
BclI T'GATC_A
Cuts at: 1548 3204 1548
Size: 1656 4360
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2813 4627 136
Size: 107 122 71 631 605 1141 1814 1525
Fragments arranged by size:
1814 1525 1141 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6011 6011
Size: 6016
BlpI GC'TnA_GC
Cuts at: 3433 3433
Size: 6016
Bme1580I G_kGCm'C
Cuts at: 1790 2019 2064 2844 2895 2915 4077 5233 1790
Size: 229 45 780 51 20 1162 1156 2573
Fragments arranged by size:
2573 1162 1156 780 229 51 45 20
BmgBI CAC'GTC
Cuts at: 1318 2494 1318
Size: 1176 4840
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2482 3747 4705
Size: 416 53 83 399 24 1065 1265 958
Cuts at: 4705 442
Size: 1753
Fragments arranged by size:
1753 1265 1065 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2715 2715
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2297 3149 4676 1239
Size: 1058 852 1527 2579
Fragments arranged by size:
2579 1527 1058 852
Bpu10I CC'TnA_GC
Cuts at: 2099 2438 2481 2099
Size: 339 43 5634
Fragments arranged by size:
5634 339 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2861 4141 5006 5304 2861
Size: 1280 865 298 3573
Fragments arranged by size:
3573 1280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4679 882
Size: 617 3180 2219
Fragments arranged by size:
3180 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3807 493
Size: 711 2603 2702
Fragments arranged by size:
2702 2603 711
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2162 2177 2196 3823
Size: 53 83 186 936 629 15 19 1627
Cuts at: 3823 4205 4840 275
Size: 382 635 1451
Fragments arranged by size:
1627 1451 936 635 629 382 186 83
53 19 15
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2149 2179 1178
Size: 30 165 30 746 30 5015
Fragments arranged by size:
5015 746 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2933 2963 1186
Size: 1747 30 4239
Fragments arranged by size:
4239 1747 30
BseYI C'CCAG_C
Cuts at: 2039 2109 2119 2351 2404 2632 2981 2997 3002
Size: 70 10 232 53 228 349 16 5
Cuts at: 3002 3072 5219 5715 2039
Size: 70 2147 496 2340
Fragments arranged by size:
2340 2147 496 349 232 228 70 70
53 16 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2516 2945 3211 1163
Size: 573 780 429 266 3968
Fragments arranged by size:
3968 780 573 429 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 3466 4227 5255 1540
Size: 275 186 36 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 2885 4077 4162 5233 2885
Size: 1192 85 1071 3668
Fragments arranged by size:
3668 1192 1071 85
BsmI GAATG_Cn'
Cuts at: 3527 3620 3527
Size: 93 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2064 2075 2844 2885 2895 2915
Size: 399 229 45 11 769 41 10 20
Cuts at: 2915 3036 3335 4077 4162 5233 1391
Size: 121 299 742 85 1071 2174
Fragments arranged by size:
2174 1071 769 742 399 299 229 121
85 45 41 20 11 10
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 3905 5635 3905
Size: 1730 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2195 2871 3371 844
Size: 1351 676 500 3489
Fragments arranged by size:
3489 1351 676 500
BsrBI CCG'CTC
Cuts at: 1394 1823 3903 1394
Size: 429 2080 3507
Fragments arranged by size:
3507 2080 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4511 4685 65
Size: 1183 3263 174 1396
Fragments arranged by size:
3263 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2239 2247 2254 4660 4847 1700
Size: 110 429 8 7 2406 187 2869
Fragments arranged by size:
2869 2406 429 187 110 8 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 4076 5088 4076
Size: 1012 5004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6016
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstXI CCAn_nnnn'nTGG
Cuts at: 2530 2530
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 2272 3346 3713 4098 4115 5567 5653 5665
Size: 300 1074 367 385 17 1452 86 12
Cuts at: 5665 6011 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 300 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2206 513
Size: 552 1141 4323
Fragments arranged by size:
4323 1141 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2081 2245 487
Size: 683 56 855 164 4258
Fragments arranged by size:
4258 855 683 164 56
BtsI GCAGTG_nn'
Cuts at: 3532 4326 4346 3532
Size: 794 20 5202
Fragments arranged by size:
5202 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2087 2256
Size: 54 1425 50 275 18 204 53 169
Cuts at: 2256 3463 4352 5981 8
Size: 1207 889 1629 43
Fragments arranged by size:
1629 1425 1207 889 275 204 169 54
53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 3463 1537
Size: 275 222 1429 4090
Fragments arranged by size:
4090 1429 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 3284 3946 1353
Size: 1931 662 3423
Fragments arranged by size:
3423 1931 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4603 4977 5123 387
Size: 1064 138 3014 374 146 1280
Fragments arranged by size:
3014 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2666 3149 4079 4676 5463 5731
Size: 393 665 369 483 930 597 787 268
Cuts at: 5731 1239
Size: 1524
Fragments arranged by size:
1524 930 787 665 597 483 393 369
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2720 2720
Size: 6016
EcoO109I rG'GnC_Cy
Cuts at: 2015 2016 2458 2815 2891 3079 3301 2015
Size: 1 442 357 76 188 222 4730
Fragments arranged by size:
4730 442 357 222 188 76 1
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
EcoRV GAT'ATC
Cuts at: 2767 2767
Size: 6016
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 2165 2180 3065 5163 1678
Size: 487 15 885 2098 2531
Fragments arranged by size:
2531 2098 885 487 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 4657 4689 4731
Size: 32 135 32 577 32 2476 32 42
Cuts at: 4731 4763 1373
Size: 32 2626
Fragments arranged by size:
2626 2476 577 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3360 3606 669
Size: 988 281 1422 246 3079
Fragments arranged by size:
3079 1422 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KasI G'GCGC_C
Cuts at: 2161 2176 2161
Size: 15 6001
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5130 5314 1305
Size: 3825 184 2007
Fragments arranged by size:
3825 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2089 10
Size: 54 1425 343 257 3937
Fragments arranged by size:
3937 1425 343 257 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 2525 3974 4333 4492 518
Size: 648 1170 189 1449 359 159 2042
Fragments arranged by size:
2042 1449 1170 648 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2146 2174 2480 2632 3432 4109 5257
Size: 433 107 28 306 152 800 677 1148
Cuts at: 5257 5502 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 800 677 433 306 245 152
107 28
NaeI GCC'GGC
Cuts at: 2241 2249 2256 2241
Size: 8 7 6001
Fragments arranged by size:
6001 8 7
NarI GG'CG_CC
Cuts at: 2162 2177 2162
Size: 15 6001
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5464
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NgoMIV G'CCGG_C
Cuts at: 2239 2247 2254 2239
Size: 8 7 6001
Fragments arranged by size:
6001 8 7
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 3374 4919 1132
Size: 97 2145 1545 2229
Fragments arranged by size:
2229 2145 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6016
PfoI T'CCnGG_A
Cuts at: 2460 2460
Size: 6016
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 4085 4117 5598 5630 3152
Size: 32 901 32 1481 32 3538
Fragments arranged by size:
3538 1481 901 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2458 3301 2458
Size: 843 5173
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PspOMI G'GGCC_C
Cuts at: 2015 2891 2015
Size: 876 5140
PstI C_TGCA'G
Cuts at: 830 2868 3153 3368 830
Size: 2038 285 215 3478
Fragments arranged by size:
3478 2038 285 215
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
PvuII CAG'CTG
Cuts at: 2632 2632
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2864 3149 3364 4499 5180 5371 826
Size: 219 1819 285 215 1135 681 191 1471
Fragments arranged by size:
1819 1471 1135 681 285 219 215 191
SfoI GGC'GCC
Cuts at: 2163 2178 2163
Size: 15 6001
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 2021 2377 3343 1872
Size: 149 356 966 4545
Fragments arranged by size:
4545 966 356 149
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2876 4120 5021 5283 820
Size: 197 926 933 1244 901 262 1553
Fragments arranged by size:
1553 1244 933 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 2672 1517
Size: 1155 4861
StyI C'CwwG_G
Cuts at: 513 1065 2025 2319 2673 2887 3105 3222 3295
Size: 552 960 294 354 214 218 117 73
Cuts at: 3295 3455 513
Size: 160 3074
Fragments arranged by size:
3074 960 552 354 294 218 214 160
117 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2900 3156 4043 4060 4213 4398 4843
Size: 1255 1320 256 887 17 153 185 445
Cuts at: 4843 325
Size: 1498
Fragments arranged by size:
1498 1320 1255 887 445 256 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4262 5974 96
Size: 274 80 33 51 1443 2285 1712 138
Fragments arranged by size:
2285 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5976 6008 5976
Size: 32 5984
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 2740 2266
Size: 474 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 2019 2375 3341 1870
Size: 149 356 966 4545
Fragments arranged by size:
4545 966 356 149
XmnI GAAnn'nnTTC
Cuts at: 4145 4145
Size: 6016
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI