Restriction map for
pFN21AB7744
(Circular) (Six-base) MAPSORT of: pFN21AB7744.seq Check: 847 from: 1 to: 6166
pFN21AB7744 6166 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2138 3270 2138
Size: 1132 5034
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3976 278
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3509 1937
Size: 1572 4594
Acc65I G'GTAC_C
Cuts at: 3487 3487
Size: 6166
AclI AA'CG_TT
Cuts at: 1563 4293 4666 1563
Size: 2730 373 3063
Fragments arranged by size:
3063 2730 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2396 4229 5613 5881 1632
Size: 635 129 1833 1384 268 1917
Fragments arranged by size:
1917 1833 1384 635 268 129
AfeI AGC'GCT
Cuts at: 3230 3230
Size: 6166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5969
AflIII A'CryG_T
Cuts at: 1203 5065 6042 1203
Size: 3862 977 1327
Fragments arranged by size:
3862 1327 977
AgeI A'CCGG_T
Cuts at: 4997 4997
Size: 6166
AhdI GACnn_n'nnGTC
Cuts at: 4895 4895
Size: 6166
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6166
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2871 2903 2871
Size: 32 6134
AlwNI CAG_nnn'CTG
Cuts at: 2164 2227 2317 3909 5481 2164
Size: 63 90 1592 1572 2849
Fragments arranged by size:
2849 1592 1572 90 63
ApaI G_GGCC'C
Cuts at: 2978 2978
Size: 6166
ApaLI G'TGCA_C
Cuts at: 4223 5379 4223
Size: 1156 5010
ApoI r'AATT_y
Cuts at: 1493 3475 3701 3853 5962 1493
Size: 1982 226 152 2109 1697
Fragments arranged by size:
2109 1982 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4720 160
Size: 872 3688 1606
Fragments arranged by size:
3688 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
AvaI C'yCGr_G
Cuts at: 1870 1943 2340 2528 3234 3412 3491 1870
Size: 73 397 188 706 178 79 4545
Fragments arranged by size:
4545 706 397 188 178 79 73
AvrII C'CTAG_G
Cuts at: 2738 2738
Size: 6166
BamHI G'GATC_C
Cuts at: 2886 3496 3863 2886
Size: 610 367 5189
Fragments arranged by size:
5189 610 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3487 4942 618
Size: 325 217 627 1700 1455 1842
Fragments arranged by size:
1842 1700 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2188 2341 2669 2919 2978 3485 1391
Size: 797 153 328 250 59 507 4072
Fragments arranged by size:
4072 797 507 328 250 153 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5195
BbvCI CC'TCA_GC
Cuts at: 2754 3225 2754
Size: 471 5695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4357 4391 1903
Size: 34 2420 34 3678
Fragments arranged by size:
3678 2420 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4058 5268 4058
Size: 1210 4956
BclI T'GATC_A
Cuts at: 1548 2100 3013 1548
Size: 552 913 4701
Fragments arranged by size:
4701 913 552
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 2175 3424
Size: 107 122 71 631 605 368 135 1249
Cuts at: 3424 4777 136
Size: 1353 1525
Fragments arranged by size:
1525 1353 1249 631 605 368 135 122
107 71
BglII A'GATC_T
Cuts at: 6161 6161
Size: 6166
BlpI GC'TnA_GC
Cuts at: 2556 2987 3583 2556
Size: 431 596 5139
Fragments arranged by size:
5139 596 431
Bme1580I G_kGCm'C
Cuts at: 1790 2978 3024 4227 5383 1790
Size: 1188 46 1203 1156 2573
Fragments arranged by size:
2573 1203 1188 1156 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6166
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2438 3897 4855
Size: 416 53 83 399 24 1021 1459 958
Cuts at: 4855 442
Size: 1753
Fragments arranged by size:
1753 1459 1021 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2066 2147 1056
Size: 1010 81 5075
Fragments arranged by size:
5075 1010 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 3438 2422
Size: 1016 5150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2286 2353 2460 2874 2929 2941 4826 1239
Size: 1047 67 107 414 55 12 1885 2579
Fragments arranged by size:
2579 1885 1047 414 107 67 55 12
Bpu10I CC'TnA_GC
Cuts at: 2273 2754 3225 2273
Size: 481 471 5214
Fragments arranged by size:
5214 481 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2392 3140 4291 5156 5454 2392
Size: 748 1151 865 298 3104
Fragments arranged by size:
3104 1151 865 748 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3117 4829 882
Size: 617 1618 1712 2219
Fragments arranged by size:
2219 1712 1618 617
BsaAI yAC'GTr
Cuts at: 493 1204 2643 3957 493
Size: 711 1439 1314 2702
Fragments arranged by size:
2702 1439 1314 711
BsaBI GATnn'nnATC
Cuts at: 6160 6160
Size: 6166
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3973 4355 4990 275
Size: 53 83 186 936 2440 382 635 1451
Fragments arranged by size:
2440 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4599 4997 5271 5418 5829 4599
Size: 398 274 147 411 4936
Fragments arranged by size:
4936 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3278 3308 1178
Size: 30 165 30 1875 30 4036
Fragments arranged by size:
4036 1875 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 2932 3397 3400 1186
Size: 1219 527 465 3 3952
Fragments arranged by size:
3952 1219 527 465 3
BseYI C'CCAG_C
Cuts at: 2030 2164 2220 2707 3317 3359 5369 5865 2030
Size: 134 56 487 610 42 2010 496 2331
Fragments arranged by size:
2331 2010 610 496 487 134 56 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2330 1163
Size: 573 594 4999
Fragments arranged by size:
4999 594 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3616 4377 5405 1540
Size: 275 186 1615 761 1028 2301
Fragments arranged by size:
2301 1615 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2640 3131 4227 4312 5383 2640
Size: 491 1096 85 1071 3423
Fragments arranged by size:
3423 1096 1071 491 85
BsmI GAATG_Cn'
Cuts at: 2823 3677 3770 2823
Size: 854 93 5219
Fragments arranged by size:
5219 854 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3164 3426 3164
Size: 262 5904
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2341 2640 2669 2919 2978 3024
Size: 399 398 153 299 29 250 59 46
Cuts at: 3024 3131 3485 4227 4312 5383 1391
Size: 107 354 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 354 299 250
153 107 85 59 46 29
BspEI T'CCGG_A
Cuts at: 5829 5829
Size: 6166
BspHI T'CATG_A
Cuts at: 3052 4055 5785 3052
Size: 1003 1730 3433
Fragments arranged by size:
3433 1730 1003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2138 3270 3521 844
Size: 1294 1132 251 3489
Fragments arranged by size:
3489 1294 1132 251
BsrBI CCG'CTC
Cuts at: 1394 1823 3281 3390 4053 1394
Size: 429 1458 109 663 3507
Fragments arranged by size:
3507 1458 663 429 109
BsrDI GCAATG_nn'
Cuts at: 65 1248 4661 4835 65
Size: 1183 3413 174 1396
Fragments arranged by size:
3413 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3308 4810 4997 1700
Size: 110 1498 1502 187 2869
Fragments arranged by size:
2869 1502 1498 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6166
BssSI C'ACGA_G
Cuts at: 2746 4226 5238 2746
Size: 1480 1012 3674
Fragments arranged by size:
3674 1480 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2635 3044 794
Size: 1841 409 3916
Fragments arranged by size:
3916 1841 409
BstBI TT'CG_AA
Cuts at: 4970 4970
Size: 6166
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6166
BstXI CCAn_nnnn'nTGG
Cuts at: 2302 2302
Size: 6166
BstYI r'GATC_y
Cuts at: 1972 2365 2886 3496 3863 4248 4265 5717 5803
Size: 393 521 610 367 385 17 1452 86
Cuts at: 5803 5815 6161 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 610 521 393 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5614
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5427
Fragments arranged by size:
5427 683 56
BtsI GCAGTG_nn'
Cuts at: 3682 4476 4496 3682
Size: 794 20 5352
Fragments arranged by size:
5352 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6131
DraI TTT'AAA
Cuts at: 3470 3470
Size: 6166
DraIII CAC_nnn'GTG
Cuts at: 2090 2090
Size: 6166
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5173 809
Size: 507 3857 1802
Fragments arranged by size:
3857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2078 2292 2605
Size: 54 1425 50 275 18 248 214 313
Cuts at: 2605 3246 3613 4502 6131 8
Size: 641 367 889 1629 43
Fragments arranged by size:
1629 1425 889 641 367 313 275 248
214 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3613 1537
Size: 275 1801 4090
Fragments arranged by size:
4090 1801 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 3000 4096 1353
Size: 963 684 1096 3423
Fragments arranged by size:
3423 1096 963 684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4753 5127 5273 387
Size: 1064 138 3164 374 146 1280
Fragments arranged by size:
3164 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2286 2353 2396 2460 2874 2929
Size: 393 635 19 67 43 64 414 55
Cuts at: 2929 2941 4229 4826 5613 5881 1239
Size: 12 1288 597 787 268 1524
Fragments arranged by size:
1524 1288 787 635 597 414 393 268
67 64 55 43 19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2285 2321 2955 2969 3412 2285
Size: 36 634 14 443 5039
Fragments arranged by size:
5039 634 443 36 14
EcoO109I rG'GnC_Cy
Cuts at: 2277 2298 2434 2742 2758 2974 2975 2277
Size: 21 136 308 16 216 1 5468
Fragments arranged by size:
5468 308 216 136 21 16 1
EcoRI G'AATT_C
Cuts at: 3475 3853 3475
Size: 378 5788
FspI TGC'GCA
Cuts at: 2684 4672 2684
Size: 1988 4178
HaeII r_GCGC'y
Cuts at: 1678 3232 5313 1678
Size: 1554 2081 2531
Fragments arranged by size:
2531 2081 1554
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2184 2216 2641 2673 3369
Size: 32 135 32 612 32 425 32 696
Cuts at: 3369 3401 4807 4839 4881 4913 1373
Size: 32 1406 32 42 32 2626
Fragments arranged by size:
2626 1406 696 612 425 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3510 3756 669
Size: 988 281 1572 246 3079
Fragments arranged by size:
3079 1572 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6166
HpaI GTT'AAC
Cuts at: 3756 3756
Size: 6166
KpnI G_GTAC'C
Cuts at: 3491 3491
Size: 6166
MfeI C'AATT_G
Cuts at: 3765 3765
Size: 6166
MluI A'CGCG_T
Cuts at: 6042 6042
Size: 6166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5280 5464 1305
Size: 3975 184 2007
Fragments arranged by size:
3975 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2607 10
Size: 54 1425 343 775 3569
Fragments arranged by size:
3569 1425 775 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4124 4483 4642 518
Size: 648 2958 359 159 2042
Fragments arranged by size:
2958 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2164 3291 3582 4259 5407 5652 1606
Size: 558 1127 291 677 1148 245 2120
Fragments arranged by size:
2120 1148 1127 677 558 291 245
NaeI GCC'GGC
Cuts at: 3310 3310
Size: 6166
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5614
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6166
NgoMIV G'CCGG_C
Cuts at: 3308 3308
Size: 6166
NheI G'CTAG_C
Cuts at: 1052 2062 2143 1052
Size: 1010 81 5075
Fragments arranged by size:
5075 1010 81
NotI GC'GGCC_GC
Cuts at: 3613 3613
Size: 6166
NspI r_CATG'y
Cuts at: 1132 1229 2331 3346 3524 5069 1132
Size: 97 1102 1015 178 1545 2229
Fragments arranged by size:
2229 1545 1102 1015 178 97
PasI CC'CwG_GG
Cuts at: 1348 2971 2993 1348
Size: 1623 22 4521
Fragments arranged by size:
4521 1623 22
PciI A'CATG_T
Cuts at: 5065 5065
Size: 6166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2137 2164 1108
Size: 1029 27 5110
Fragments arranged by size:
5110 1029 27
PmeI GTTT'AAAC
Cuts at: 3470 3470
Size: 6166
PmlI CAC'GTG
Cuts at: 2643 2643
Size: 6166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4235 4267 5748 5780 4235
Size: 32 1481 32 4621
Fragments arranged by size:
4621 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2434 2434
Size: 6166
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6166
PsiI TTA'TAA
Cuts at: 3736 3736
Size: 6166
PspOMI G'GGCC_C
Cuts at: 2974 2974
Size: 6166
PspXI vC'TCGA_Gb
Cuts at: 2340 3412 2340
Size: 1072 5094
PstI C_TGCA'G
Cuts at: 830 2262 3518 830
Size: 1432 1256 3478
Fragments arranged by size:
3478 1432 1256
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6166
PvuII CAG'CTG
Cuts at: 2164 2164
Size: 6166
SalI G'TCGA_C
Cuts at: 1936 3508 1936
Size: 1572 4594
SanDI GG'GwC_CC
Cuts at: 2434 2434
Size: 6166
SbfI CC_TGCA'GG
Cuts at: 3518 3518
Size: 6166
ScaI AGT'ACT
Cuts at: 4414 6126 4414
Size: 1712 4454
SexAI A'CCwGG_T
Cuts at: 2570 2570
Size: 6166
SfcI C'TryA_G
Cuts at: 826 1045 2258 3211 3514 4649 5330 5521 826
Size: 219 1213 953 303 1135 681 191 1471
Fragments arranged by size:
1471 1213 1135 953 681 303 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2175 3424 2175
Size: 1249 4917
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6166
SmaI CCC'GGG
Cuts at: 1872 3236 3493 1872
Size: 1364 257 4545
Fragments arranged by size:
4545 1364 257
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2340 2407 3155 3412 4270 5171
Size: 197 926 397 67 748 257 858 901
Cuts at: 5171 5433 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 858 748 397 262 257
197 67
SnaBI TAC'GTA
Cuts at: 493 3957 493
Size: 3464 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6166
SphI G_CATG'C
Cuts at: 1132 2331 3524 1132
Size: 1199 1193 3774
Fragments arranged by size:
3774 1199 1193
SspI AAT'ATT
Cuts at: 5 52 4090 5
Size: 47 4038 2081
Fragments arranged by size:
4038 2081 47
StuI AGG'CCT
Cuts at: 1517 2373 1517
Size: 856 5310
StyI C'CwwG_G
Cuts at: 513 1065 2301 2738 2762 3605 513
Size: 552 1236 437 24 843 3074
Fragments arranged by size:
3074 1236 843 552 437 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3099 4193 4210 4363 4548 4993 325
Size: 1255 1519 1094 17 153 185 445 1498
Fragments arranged by size:
1519 1498 1255 1094 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4412 6124 96
Size: 274 80 33 51 1443 2435 1712 138
Fragments arranged by size:
2435 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6126 6158 6126
Size: 32 6134
Tth111I GACn'n_nGTC
Cuts at: 1316 2572 1316
Size: 1256 4910
XbaI T'CTAG_A
Cuts at: 2468 3502 2468
Size: 1034 5132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2600 2600
Size: 6166
XhoI C'TCGA_G
Cuts at: 1943 2340 3412 1943
Size: 397 1072 4697
Fragments arranged by size:
4697 1072 397
XmaI C'CCGG_G
Cuts at: 1870 3234 3491 1870
Size: 1364 257 4545
Fragments arranged by size:
4545 1364 257
XmnI GAAnn'nnTTC
Cuts at: 3203 4295 3203
Size: 1092 5074
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3974 276
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PsrI RsrII SacI SacII SapI SfoI
SrfI SwaI