Restriction map for
pFN21AB7742
(Circular) (Six-base) MAPSORT of: pFN21AB7742.seq Check: 48 from: 1 to: 6163
pFN21AB7742 6163 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2773 2773
Size: 6163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3506 1937
Size: 1569 4594
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2738 3234 4226 5610 5878 1632
Size: 1106 496 992 1384 268 1917
Fragments arranged by size:
1917 1384 1106 992 496 268
AfeI AGC'GCT
Cuts at: 2629 2629
Size: 6163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5966
AflIII A'CryG_T
Cuts at: 1203 5062 6039 1203
Size: 3859 977 1327
Fragments arranged by size:
3859 1327 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 4892 4892
Size: 6163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AlwNI CAG_nnn'CTG
Cuts at: 2078 2945 3095 3906 5478 2078
Size: 867 150 811 1572 2763
Fragments arranged by size:
2763 1572 867 811 150
ApaI G_GGCC'C
Cuts at: 2235 2235
Size: 6163
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 2726 3472 3698 3850 5959 1493
Size: 1233 746 226 152 2109 1697
Fragments arranged by size:
2109 1697 1233 746 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 3119 3206 3488 1870
Size: 73 1164 12 87 282 4545
Fragments arranged by size:
4545 1164 282 87 73 12
AvrII C'CTAG_G
Cuts at: 3041 3041
Size: 6163
BamHI G'GATC_C
Cuts at: 3493 3860 3493
Size: 367 5796
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2705 3484 4939 618
Size: 325 217 627 918 779 1455 1842
Fragments arranged by size:
1842 1455 918 779 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2235 2831 3027 3108 3120 3482 1391
Size: 844 596 196 81 12 362 4072
Fragments arranged by size:
4072 844 596 362 196 81 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2681 928
Size: 971 782 4410
Fragments arranged by size:
4410 971 782
BbvCI CC'TCA_GC
Cuts at: 2174 2742 2174
Size: 568 5595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4354 4388 1903
Size: 34 2417 34 3678
Fragments arranged by size:
3678 2417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2995 4774 136
Size: 107 122 71 631 605 1323 1779 1525
Fragments arranged by size:
1779 1525 1323 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 3580 3580
Size: 6163
Bme1580I G_kGCm'C
Cuts at: 1790 2235 2710 2982 4224 5380 1790
Size: 445 475 272 1242 1156 2573
Fragments arranged by size:
2573 1242 1156 475 445 272
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2969 3894 4852
Size: 416 53 83 399 24 1552 925 958
Cuts at: 4852 442
Size: 1753
Fragments arranged by size:
1753 1552 958 925 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2901 3044 3479 4823 1239
Size: 1662 143 435 1344 2579
Fragments arranged by size:
2579 1662 1344 435 143
Bpu10I CC'TnA_GC
Cuts at: 2174 2742 3096 3102 3114 2174
Size: 568 354 6 12 5223
Fragments arranged by size:
5223 568 354 12 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4288 5153 5451 4288
Size: 865 298 5000
Fragments arranged by size:
5000 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 2271 4826 882
Size: 617 543 229 2555 2219
Fragments arranged by size:
2555 2219 617 543 229
BsaAI yAC'GTr
Cuts at: 493 1204 3954 493
Size: 711 2750 2702
Fragments arranged by size:
2750 2702 711
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2681 3970 4352 4987
Size: 53 83 186 936 1148 1289 382 635
Cuts at: 4987 275
Size: 1451
Fragments arranged by size:
1451 1289 1148 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3355 4596 4994 5268 5415 5826 3355
Size: 1241 398 274 147 411 3692
Fragments arranged by size:
3692 1241 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5938
Fragments arranged by size:
5938 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2759 2783 2839 2890 2919 3035 3164
Size: 1021 552 24 56 51 29 116 129
Cuts at: 3164 3212 1186
Size: 48 4137
Fragments arranged by size:
4137 1021 552 129 116 56 51 48
29 24
BseYI C'CCAG_C
Cuts at: 3028 3263 3269 3275 5366 5862 3028
Size: 235 6 6 2091 496 3329
Fragments arranged by size:
3329 2091 496 235 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 1163
Size: 573 580 5010
Fragments arranged by size:
5010 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3207 4224 4309 5380 3207
Size: 1017 85 1071 3990
Fragments arranged by size:
3990 1071 1017 85
BsmI GAATG_Cn'
Cuts at: 3674 3767 3674
Size: 93 6070
BsmBI CGTCTCn'nnnn_
Cuts at: 2537 2537
Size: 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2710 2831 2982 3027 3108 3120
Size: 399 445 475 121 151 45 81 12
Cuts at: 3120 3207 3482 4224 4309 5380 1391
Size: 87 275 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 445 399 275 151
121 87 85 81 45 12
BspEI T'CCGG_A
Cuts at: 3355 5826 3355
Size: 2471 3692
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2773 3518 844
Size: 1929 745 3489
Fragments arranged by size:
3489 1929 745
BsrBI CCG'CTC
Cuts at: 1394 1823 4050 1394
Size: 429 2227 3507
Fragments arranged by size:
3507 2227 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4658 4832 65
Size: 1183 3410 174 1396
Fragments arranged by size:
3410 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2481 2875 4807 4994 1700
Size: 110 671 394 1932 187 2869
Fragments arranged by size:
2869 1932 671 394 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6163
BssSI C'ACGA_G
Cuts at: 2466 2555 4223 5235 2466
Size: 89 1668 1012 3394
Fragments arranged by size:
3394 1668 1012 89
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6163
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstXI CCAn_nnnn'nTGG
Cuts at: 2252 2252
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 2074 3294 3493 3860 4245 4262 5714 5800
Size: 102 1220 199 367 385 17 1452 86
Cuts at: 5800 5812 6158 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1220 385 367 346 199 102
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2832 2832
Size: 6163
BtgI C'CryG_G
Cuts at: 513 1065 2043 2245 2710 2736 3415 513
Size: 552 978 202 465 26 679 3261
Fragments arranged by size:
3261 978 679 552 465 202 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5424
Fragments arranged by size:
5424 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 2882 3679 4473 4493 2304
Size: 578 797 794 20 3974
Fragments arranged by size:
3974 797 794 578 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6128
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DraIII CAC_nnn'GTG
Cuts at: 2977 2977
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2479 2590 2787
Size: 54 1425 50 275 18 649 111 197
Cuts at: 2787 3418 3610 4499 6128 8
Size: 631 192 889 1629 43
Fragments arranged by size:
1629 1425 889 649 631 275 197 192
111 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2341 2744 2760 2856 3066 3437 4093 1353
Size: 988 403 16 96 210 371 656 3423
Fragments arranged by size:
3423 988 656 403 371 210 96 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4750 5124 5270 387
Size: 1064 138 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2738 2901 3044 3234 3479 4226 4823
Size: 393 1106 163 143 190 245 747 597
Cuts at: 4823 5610 5878 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1106 787 747 597 393 268 245
190 163 143
EcoO109I rG'GnC_Cy
Cuts at: 2231 2653 2231
Size: 422 5741
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FspI TGC'GCA
Cuts at: 4669 4669
Size: 6163
HaeII r_GCGC'y
Cuts at: 1678 2631 5310 1678
Size: 953 2679 2531
Fragments arranged by size:
2679 2531 953
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 4804 4836 4878
Size: 32 135 32 638 32 2562 32 42
Cuts at: 4878 4910 1373
Size: 32 2626
Fragments arranged by size:
2626 2562 638 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3507 3753 669
Size: 988 281 1569 246 3079
Fragments arranged by size:
3079 1569 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3452 748
Size: 2704 3459
HpaI GTT'AAC
Cuts at: 3753 3753
Size: 6163
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5277 5461 1305
Size: 3972 184 2007
Fragments arranged by size:
3972 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2592 2789 3420 10
Size: 54 1425 343 760 197 631 2753
Fragments arranged by size:
2753 1425 760 631 343 197 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2250 2310 3380 4121 4480 4639 518
Size: 648 1084 60 1070 741 359 159 2042
Fragments arranged by size:
2042 1084 1070 741 648 359 159 60
MspA1I CmG'CkG
Cuts at: 1606 2173 3302 3579 4256 5404 5649 1606
Size: 567 1129 277 677 1148 245 2120
Fragments arranged by size:
2120 1148 1129 677 567 277 245
NcoI C'CATG_G
Cuts at: 513 1065 2245 2736 3415 513
Size: 552 1180 491 679 3261
Fragments arranged by size:
3261 1180 679 552 491
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 3521 5066 1132
Size: 97 2292 1545 2229
Fragments arranged by size:
2292 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2053 1348
Size: 705 5458
PciI A'CATG_T
Cuts at: 5062 5062
Size: 6163
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6163
PfoI T'CCnGG_A
Cuts at: 3006 3006
Size: 6163
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 2424 2670 3733 2424
Size: 246 1063 4854
Fragments arranged by size:
4854 1063 246
PspOMI G'GGCC_C
Cuts at: 2231 2231
Size: 6163
PspXI vC'TCGA_Gb
Cuts at: 3206 3206
Size: 6163
PstI C_TGCA'G
Cuts at: 830 3515 830
Size: 2685 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
PvuII CAG'CTG
Cuts at: 2173 3302 2173
Size: 1129 5034
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SapI GCTCTTCn'nnn_
Cuts at: 2856 3066 2856
Size: 210 5953
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SfcI C'TryA_G
Cuts at: 826 1045 2016 3511 4646 5327 5518 826
Size: 219 971 1495 1135 681 191 1471
Fragments arranged by size:
1495 1471 1135 971 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 3490 1872
Size: 1618 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3206 4267 5168 5430 820
Size: 197 926 1263 1061 901 262 1553
Fragments arranged by size:
1553 1263 1061 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 2909 3057 1517
Size: 1392 148 4623
Fragments arranged by size:
4623 1392 148
StyI C'CwwG_G
Cuts at: 513 1065 2245 2577 2736 3041 3322 3415 3602
Size: 552 1180 332 159 305 281 93 187
Cuts at: 3602 513
Size: 3074
Fragments arranged by size:
3074 1180 552 332 305 281 187 159
93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2295 2628 2988 4190 4207 4360 4545
Size: 1255 715 333 360 1202 17 153 185
Cuts at: 4545 4990 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1202 715 445 360 333 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4409 6121 96
Size: 274 80 33 51 1443 2432 1712 138
Fragments arranged by size:
2432 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6123 6155 6123
Size: 32 6131
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6163
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3422 3422
Size: 6163
XhoI C'TCGA_G
Cuts at: 1943 3206 1943
Size: 1263 4900
XmaI C'CCGG_G
Cuts at: 1870 3488 1870
Size: 1618 4545
XmnI GAAnn'nnTTC
Cuts at: 4292 4292
Size: 6163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI