Restriction map for
pFN21AB7740
(Circular) (Six-base) MAPSORT of: pFN21AB7740.seq Check: 444 from: 1 to: 6151
pFN21AB7740 6151 bp
With 182 enzymes: SgfI *
July 14, 2009 10:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2581 3494 1937
Size: 644 913 4594
Fragments arranged by size:
4594 913 644
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 2470 3239 4214 5598 5866 1632
Size: 654 184 769 975 1384 268 1917
Fragments arranged by size:
1917 1384 975 769 654 268 184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AlwNI CAG_nnn'CTG
Cuts at: 2155 3894 5466 2155
Size: 1739 1572 2840
Fragments arranged by size:
2840 1739 1572
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2634 2850 2864 2881 2933 3087 3460 3686
Size: 1141 216 14 17 52 154 373 226
Cuts at: 3686 3838 5947 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1141 373 226 216 154 152
52 17 14
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 3476 1870
Size: 73 1533 4545
Fragments arranged by size:
4545 1533 73
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3472 4927 618
Size: 325 217 627 1685 1455 1842
Fragments arranged by size:
1842 1685 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3470 1391
Size: 2079 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2706 2982 928
Size: 971 807 276 4097
Fragments arranged by size:
4097 971 807 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2435 4043 5253 2435
Size: 1608 1210 3333
Fragments arranged by size:
3333 1608 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4762 136
Size: 107 122 71 631 605 3090 1525
Fragments arranged by size:
3090 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 3568 3568
Size: 6151
Bme1580I G_kGCm'C
Cuts at: 1790 4212 5368 1790
Size: 2422 1156 2573
Fragments arranged by size:
2573 2422 1156
BmgBI CAC'GTC
Cuts at: 1318 3445 1318
Size: 2127 4024
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3882 4840 442
Size: 416 53 83 399 24 2465 958 1753
Fragments arranged by size:
2465 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2847 2293
Size: 554 5597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3195 4811 1239
Size: 1956 1616 2579
Fragments arranged by size:
2579 1956 1616
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3153 4276 5141 5439 3153
Size: 1123 865 298 3865
Fragments arranged by size:
3865 1123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4814 882
Size: 617 3315 2219
Fragments arranged by size:
3315 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 6145 6145
Size: 6151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3958 4340 4975 275
Size: 53 83 186 936 2425 382 635 1451
Fragments arranged by size:
2425 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5926
Fragments arranged by size:
5926 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3188 1186
Size: 2002 4149
BseYI C'CCAG_C
Cuts at: 2256 5354 5850 2256
Size: 3098 496 2557
Fragments arranged by size:
3098 2557 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2892 3467 1163
Size: 573 1156 575 3847
Fragments arranged by size:
3847 1156 575 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4212 4297 5368 4212
Size: 85 1071 4995
Fragments arranged by size:
4995 1071 85
BsmI GAATG_Cn'
Cuts at: 2624 3064 3662 3755 2624
Size: 440 598 93 5020
Fragments arranged by size:
5020 598 440 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3470 4212 4297 5368 1391
Size: 399 1680 742 85 1071 2174
Fragments arranged by size:
2174 1680 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 4040 5770 4040
Size: 1730 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2126 3439 3506 844
Size: 1282 1313 67 3489
Fragments arranged by size:
3489 1313 1282 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4038 1394
Size: 429 2215 3507
Fragments arranged by size:
3507 2215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2191 4646 4820 65
Size: 1183 943 2455 174 1396
Fragments arranged by size:
2455 1396 1183 943 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4795 4982 1700
Size: 110 2985 187 2869
Fragments arranged by size:
2985 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2514 2553 2917 96
Size: 2418 39 364 3330
Fragments arranged by size:
3330 2418 364 39
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6151
BstBI TT'CG_AA
Cuts at: 3091 4955 3091
Size: 1864 4287
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstYI r'GATC_y
Cuts at: 1972 3481 3848 4233 4250 5702 5788 5800 6146
Size: 1509 367 385 17 1452 86 12 346
Cuts at: 6146 1972
Size: 1977
Fragments arranged by size:
1977 1509 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2832 2832
Size: 6151
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 2053 3667 4461 4481 2053
Size: 1614 794 20 3723
Fragments arranged by size:
3723 1614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 2856 3455 2856
Size: 599 5552
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2743 5158 809
Size: 507 1427 2415 1802
Fragments arranged by size:
2415 1802 1427 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116
Size: 54 1425 50 275 18 1768 889 1629
Cuts at: 6116 8
Size: 43
Fragments arranged by size:
1768 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 2793 4081 1353
Size: 909 531 1288 3423
Fragments arranged by size:
3423 1288 909 531
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4738 5112 5258 387
Size: 1064 138 3149 374 146 1280
Fragments arranged by size:
3149 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2470 3195 3239 4214 4811 5598
Size: 393 654 184 725 44 975 597 787
Cuts at: 5598 5866 1239
Size: 268 1524
Fragments arranged by size:
1524 975 787 725 654 597 393 268
184 44
EcoO109I rG'GnC_Cy
Cuts at: 2138 2138
Size: 6151
EcoRI G'AATT_C
Cuts at: 2864 2933 3087 3460 3838 2864
Size: 69 154 373 378 5177
Fragments arranged by size:
5177 378 373 154 69
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 5298 1678
Size: 3620 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 3349 3381 4792
Size: 32 135 32 848 32 897 32 1411
Cuts at: 4792 4824 4866 4898 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1411 897 848 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2582 2628 3495 3741 669
Size: 988 281 644 46 867 246 3079
Fragments arranged by size:
3079 988 867 644 281 246 46
HindIII A'AGCT_T
Cuts at: 748 3251 748
Size: 2503 3648
HpaI GTT'AAC
Cuts at: 2628 3741 2628
Size: 1113 5038
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3195 3750 3195
Size: 555 5596
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2262 5265 5449 1305
Size: 957 3003 184 2007
Fragments arranged by size:
3003 2007 957 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4329
Fragments arranged by size:
4329 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2087 2451 4109 4468 4627 518
Size: 648 843 78 364 1658 359 159 2042
Fragments arranged by size:
2042 1658 843 648 364 359 159 78
MspA1I CmG'CkG
Cuts at: 1606 2061 2312 3032 3567 4244 5392 5637 1606
Size: 455 251 720 535 677 1148 245 2120
Fragments arranged by size:
2120 1148 720 677 535 455 251 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2560 2681 3509 5054 1132
Size: 97 1331 121 828 1545 2229
Fragments arranged by size:
2229 1545 1331 828 121 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6151
PfoI T'CCnGG_A
Cuts at: 2281 2281
Size: 6151
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2138 2138
Size: 6151
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3141 3721 3141
Size: 580 5571
PstI C_TGCA'G
Cuts at: 830 2060 3503 830
Size: 1230 1443 3478
Fragments arranged by size:
3478 1443 1230
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 2061 2312 3032 2061
Size: 251 720 5180
Fragments arranged by size:
5180 720 251
SalI G'TCGA_C
Cuts at: 1936 2580 3493 1936
Size: 644 913 4594
Fragments arranged by size:
4594 913 644
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SfcI C'TryA_G
Cuts at: 826 1045 2056 3499 4634 5315 5506 826
Size: 219 1011 1443 1135 681 191 1471
Fragments arranged by size:
1471 1443 1135 1011 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3168 4255 5156 5418 820
Size: 197 926 1225 1087 901 262 1553
Fragments arranged by size:
1553 1225 1087 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 2681 3509 1132
Size: 1549 828 3774
Fragments arranged by size:
3774 1549 828
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2004 3590 513
Size: 552 939 1586 3074
Fragments arranged by size:
3074 1586 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4178 4195 4348 4533 4978 325
Size: 1255 2598 17 153 185 445 1498
Fragments arranged by size:
2598 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 2553 2917
Size: 274 80 33 51 1443 537 39 364
Cuts at: 2917 4397 6109 96
Size: 1480 1712 138
Fragments arranged by size:
1712 1480 1443 537 364 274 138 80
51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2396 2428 6111 6143 2396
Size: 32 3683 32 2404
Fragments arranged by size:
3683 2404 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6151
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 2883 3087 4280 2883
Size: 204 1193 4754
Fragments arranged by size:
4754 1193 204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI