Restriction map for
pFN21AB7739
(Circular) (Six-base) MAPSORT of: pFN21AB7739.seq Check: 2432 from: 1 to: 6013
pFN21AB7739 6013 bp
With 182 enzymes: SgfI *
March 28, 2012 12:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3049 3049
Size: 6013
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2865 3356 1937
Size: 928 491 4594
Fragments arranged by size:
4594 928 491
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2049 2384 2573 2988 4076 5460 5728 1632
Size: 417 335 189 415 1088 1384 268 1917
Fragments arranged by size:
1917 1384 1088 417 415 335 268 189
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 3109 4912 5889 1203
Size: 1906 1803 977 1327
Fragments arranged by size:
1906 1803 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AlwNI CAG_nnn'CTG
Cuts at: 3756 5328 3756
Size: 1572 4441
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 3322 3548 3700 5809 1493
Size: 1829 226 152 2109 1697
Fragments arranged by size:
2109 1829 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2058 4567 160
Size: 872 1026 2509 1606
Fragments arranged by size:
2509 1606 1026 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 3338 1870
Size: 73 1395 4545
Fragments arranged by size:
4545 1395 73
BamHI G'GATC_C
Cuts at: 3343 3710 3343
Size: 367 5646
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 3334 4789 618
Size: 325 217 627 379 1168 1455 1842
Fragments arranged by size:
1842 1455 1168 627 379 325 217
BanII G_rGCy'C
Cuts at: 1391 3332 1391
Size: 1941 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 3168 928
Size: 971 138 1131 3773
Fragments arranged by size:
3773 1131 971 138
BbvCI CC'TCA_GC
Cuts at: 2493 2685 2493
Size: 192 5821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 2461 2872 1548
Size: 913 411 4689
Fragments arranged by size:
4689 913 411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2283 2749 6008 2283
Size: 466 3259 2288
Fragments arranged by size:
3259 2288 466
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 4074 5230 1790
Size: 2284 1156 2573
Fragments arranged by size:
2573 2284 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6013
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3744 4702 442
Size: 416 53 83 399 24 2327 958 1753
Fragments arranged by size:
2327 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2434 4673 1239
Size: 1195 2239 2579
Fragments arranged by size:
2579 2239 1195
Bpu10I CC'TnA_GC
Cuts at: 2171 2493 2685 2171
Size: 322 192 5499
Fragments arranged by size:
5499 322 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 3141 4138 5003 5301 2204
Size: 937 997 865 298 2916
Fragments arranged by size:
2916 997 937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2287 2561 2580 2829 4676 882
Size: 617 788 274 19 249 1847 2219
Fragments arranged by size:
2219 1847 788 617 274 249 19
BsaAI yAC'GTr
Cuts at: 493 1204 3804 493
Size: 711 2600 2702
Fragments arranged by size:
2702 2600 711
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2637 3820 4202 4837
Size: 53 83 186 936 1104 1183 382 635
Cuts at: 4837 275
Size: 1451
Fragments arranged by size:
1451 1183 1104 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2858 4446 4844 5118 5265 5676 2858
Size: 1588 398 274 147 411 3195
Fragments arranged by size:
3195 1588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 1178
Size: 30 165 30 789 30 4969
Fragments arranged by size:
4969 789 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2673 1186
Size: 1099 388 4526
Fragments arranged by size:
4526 1099 388
BseYI C'CCAG_C
Cuts at: 2049 2178 5216 5712 2049
Size: 129 3038 496 2350
Fragments arranged by size:
3038 2350 496 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5440
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2254 3463 4224 5252 1540
Size: 275 186 253 1209 761 1028 2301
Fragments arranged by size:
2301 1209 1028 761 275 253 186
BsiHKAI G_wGCw'C
Cuts at: 2564 4074 4159 5230 2564
Size: 1510 85 1071 3347
Fragments arranged by size:
3347 1510 1071 85
BsmI GAATG_Cn'
Cuts at: 2161 3524 3617 2161
Size: 1363 93 4557
Fragments arranged by size:
4557 1363 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2545 2545
Size: 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2564 3332 4074 4159 5230 1391
Size: 399 774 768 742 85 1071 2174
Fragments arranged by size:
2174 1071 774 768 742 399 85
BspEI T'CCGG_A
Cuts at: 2858 5676 2858
Size: 2818 3195
BspHI T'CATG_A
Cuts at: 3902 5632 3902
Size: 1730 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2305 2847 3049 3368 844
Size: 1461 542 202 319 3489
Fragments arranged by size:
3489 1461 542 319 202
BsrBI CCG'CTC
Cuts at: 1394 1823 3900 1394
Size: 429 2077 3507
Fragments arranged by size:
3507 2077 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4508 4682 65
Size: 1183 3260 174 1396
Fragments arranged by size:
3260 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4657 4844 1700
Size: 110 2847 187 2869
Fragments arranged by size:
2869 2847 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6013
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 2401 1184
Size: 1217 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 2648 2648
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 2283 2749 3343 3710 4095 4112 5564 5650
Size: 311 466 594 367 385 17 1452 86
Cuts at: 5650 5662 6008 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 594 466 385 367 346 311
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4522
Fragments arranged by size:
4522 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 3209 3271 3529 4323 4343 3209
Size: 62 258 794 20 4879
Fragments arranged by size:
4879 794 258 62 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2111 2251 3071
Size: 54 1425 50 275 18 281 140 820
Cuts at: 3071 3460 4349 5978 8
Size: 389 889 1629 43
Fragments arranged by size:
1629 1425 889 820 389 281 275 140
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2251 3460 1537
Size: 275 439 1209 4090
Fragments arranged by size:
4090 1209 439 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2497 2583 3007 3093 3179 3943 1353
Size: 1144 86 424 86 86 764 3423
Fragments arranged by size:
3423 1144 764 424 86 86 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2384 2434 2573 2988 4076 4673
Size: 393 417 335 50 139 415 1088 597
Cuts at: 4673 5460 5728 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1088 787 597 417 415 393 335
268 139 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2379 2379
Size: 6013
EcoRI G'AATT_C
Cuts at: 3322 3700 3322
Size: 378 5635
FspI TGC'GCA
Cuts at: 2160 2189 4519 2160
Size: 29 2330 3654
Fragments arranged by size:
3654 2330 29
HaeII r_GCGC'y
Cuts at: 1678 5160 1678
Size: 3482 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2286 2318 2558 2560 2590
Size: 32 135 32 714 32 240 2 30
Cuts at: 2590 2592 2716 2748 2908 2940 4654 4686 4728
Size: 2 124 32 160 32 1714 32 42
Cuts at: 4728 4760 1373
Size: 32 2626
Fragments arranged by size:
2626 1714 714 240 160 135 124 42
32 32 32 32 32 32 32 30
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 2642 3612 2642
Size: 970 5043
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5127 5311 1305
Size: 3822 184 2007
Fragments arranged by size:
3822 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2113 3073 10
Size: 54 1425 343 281 960 2950
Fragments arranged by size:
2950 1425 960 343 281 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3971 4330 4489 518
Size: 648 2805 359 159 2042
Fragments arranged by size:
2805 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2164 2178 2332 2970 3083 3429 4106 5254
Size: 558 14 154 638 113 346 677 1148
Cuts at: 5254 5499 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 638 558 346 245 154
113 14
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4522
Fragments arranged by size:
4522 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 2251 3460 2251
Size: 1209 4804
NspI r_CATG'y
Cuts at: 1132 1229 3113 3371 4916 1132
Size: 97 1884 258 1545 2229
Fragments arranged by size:
2229 1884 1545 258 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6013
PciI A'CATG_T
Cuts at: 3109 4912 3109
Size: 1803 4210
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2412 1108
Size: 1304 4709
PfoI T'CCnGG_A
Cuts at: 2103 2628 2103
Size: 525 5488
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5595 5627 4082
Size: 32 1481 32 4468
Fragments arranged by size:
4468 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PstI C_TGCA'G
Cuts at: 830 3046 3365 830
Size: 2216 319 3478
Fragments arranged by size:
3478 2216 319
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
PvuII CAG'CTG
Cuts at: 2178 2970 2178
Size: 792 5221
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SapI GCTCTTCn'nnn_
Cuts at: 2497 2497
Size: 6013
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SexAI A'CCwGG_T
Cuts at: 2570 2570
Size: 6013
SfcI C'TryA_G
Cuts at: 826 1045 2137 2824 3042 3361 4496 5177 5368
Size: 219 1092 687 218 319 1135 681 191
Cuts at: 5368 826
Size: 1471
Fragments arranged by size:
1471 1135 1092 687 681 319 219 218
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2219 3156 4117 5018 5280 820
Size: 197 926 276 937 961 901 262 1553
Fragments arranged by size:
1553 961 937 926 901 276 262 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 3206 3937 5
Size: 47 3154 731 2081
Fragments arranged by size:
3154 2081 731 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6013
StyI C'CwwG_G
Cuts at: 513 1065 2004 3452 513
Size: 552 939 1448 3074
Fragments arranged by size:
3074 1448 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2225 4040 4057 4210 4395 4840 325
Size: 1255 645 1815 17 153 185 445 1498
Fragments arranged by size:
1815 1498 1255 645 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3106 4259 5971
Size: 274 80 33 51 1443 1129 1153 1712
Cuts at: 5971 96
Size: 138
Fragments arranged by size:
1712 1443 1153 1129 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 2572 1316
Size: 1256 4757
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 4142 4142
Size: 6013
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI