Restriction map for
pFN21AB7737
(Circular) (Six-base) MAPSORT of: pFN21AB7737.seq Check: 9001 from: 1 to: 6013
pFN21AB7737 6013 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2520 3356 1937
Size: 583 836 4594
Fragments arranged by size:
4594 836 583
Acc65I G'GTAC_C
Cuts at: 3195 3217 3334 3195
Size: 22 117 5874
Fragments arranged by size:
5874 117 22
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2804 4076 5460 5728 1632
Size: 1172 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1272 1172 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 4912 5889 1203
Size: 3709 977 1327
Fragments arranged by size:
3709 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 3022 1166
Size: 1856 4157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AlwNI CAG_nnn'CTG
Cuts at: 2390 2835 3756 5328 2390
Size: 445 921 1572 3075
Fragments arranged by size:
3075 1572 921 445
ApaI G_GGCC'C
Cuts at: 2061 2882 3082 3251 2061
Size: 821 200 169 4823
Fragments arranged by size:
4823 821 200 169
ApaLI G'TGCA_C
Cuts at: 2550 4070 5226 2550
Size: 1520 1156 3337
Fragments arranged by size:
3337 1520 1156
ApoI r'AATT_y
Cuts at: 1493 3322 3548 3700 5809 1493
Size: 1829 226 152 2109 1697
Fragments arranged by size:
2109 1829 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2269 2402 2729 2993 3338 1870
Size: 73 326 133 327 264 345 4545
Fragments arranged by size:
4545 345 327 326 264 133 73
BamHI G'GATC_C
Cuts at: 3343 3710 3343
Size: 367 5646
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3016 3195 3217 3334 4789
Size: 325 217 627 1229 179 22 117 1455
Cuts at: 4789 618
Size: 1842
Fragments arranged by size:
1842 1455 1229 627 325 217 179 117
22
BanII G_rGCy'C
Cuts at: 1391 2061 2452 2484 2502 2882 3082 3107 3191
Size: 670 391 32 18 380 200 25 84
Cuts at: 3191 3251 3299 3332 1391
Size: 60 48 33 4072
Fragments arranged by size:
4072 670 391 380 200 84 60 48
33 32 25 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5042
BbvCI CC'TCA_GC
Cuts at: 3042 3042
Size: 6013
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2522 2556 4204 4238 1903
Size: 34 585 34 1648 34 3678
Fragments arranged by size:
3678 1648 585 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 2337 1548
Size: 789 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2900 4624 136
Size: 107 122 71 631 605 1228 1724 1525
Fragments arranged by size:
1724 1525 1228 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6008 6008
Size: 6013
BlpI GC'TnA_GC
Cuts at: 3139 3430 3139
Size: 291 5722
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2228 2554 2882 3082 3251 4074 5230
Size: 271 167 326 328 200 169 823 1156
Cuts at: 5230 1790
Size: 2573
Fragments arranged by size:
2573 1156 823 328 326 271 200 169
167
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6013
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3030 3078
Size: 416 53 83 399 24 1293 320 48
Cuts at: 3078 3744 4702 442
Size: 666 958 1753
Fragments arranged by size:
1753 1293 958 666 416 399 320 83
53 48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 2232 2309 3108 3185 4673 1239
Size: 826 167 77 799 77 1488 2579
Fragments arranged by size:
2579 1488 826 799 167 77 77
Bpu10I CC'TnA_GC
Cuts at: 2282 3042 2282
Size: 760 5253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4138 5003 5301 4138
Size: 865 298 4850
Fragments arranged by size:
4850 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2726 4676 882
Size: 617 1227 1950 2219
Fragments arranged by size:
2219 1950 1227 617
BsaAI yAC'GTr
Cuts at: 493 1204 3804 493
Size: 711 2600 2702
Fragments arranged by size:
2702 2600 711
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2853 3820 4202 4837
Size: 53 83 186 936 1320 967 382 635
Cuts at: 4837 275
Size: 1451
Fragments arranged by size:
1451 1320 967 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2437 4446 4844 5118 5265 5676 2437
Size: 2009 398 274 147 411 2774
Fragments arranged by size:
2774 2009 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3222 3252 1178
Size: 30 165 30 1819 30 3939
Fragments arranged by size:
3939 1819 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6013
BseYI C'CCAG_C
Cuts at: 2351 2475 2483 2682 2986 3107 3199 5216 5712
Size: 124 8 199 304 121 92 2017 496
Cuts at: 5712 2351
Size: 2652
Fragments arranged by size:
2652 2017 496 304 199 124 121 92
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2618 3041 1163
Size: 573 882 423 4135
Fragments arranged by size:
4135 882 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3463 4224 5252 1540
Size: 275 186 1462 761 1028 2301
Fragments arranged by size:
2301 1462 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2554 4074 4159 5230 2554
Size: 1520 85 1071 3337
Fragments arranged by size:
3337 1520 1071 85
BsmI GAATG_Cn'
Cuts at: 3524 3617 3524
Size: 93 5920
BsmBI CGTCTCn'nnnn_
Cuts at: 2844 2844
Size: 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2228 2452 2484 2502 2554 2882
Size: 399 271 167 224 32 18 52 328
Cuts at: 2882 3082 3107 3191 3251 3299 3332 4074 4159
Size: 200 25 84 60 48 33 742 85
Cuts at: 4159 5230 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 271 224 200
167 85 84 60 52 48 33 32
25 18
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 3902 5632 3902
Size: 1730 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3368 844
Size: 2524 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2913 3900 1394
Size: 429 1090 987 3507
Fragments arranged by size:
3507 1090 987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4508 4682 65
Size: 1183 3260 174 1396
Fragments arranged by size:
3260 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2233 2469 3211 4657 4844 1700
Size: 110 232 191 236 742 1446 187 2869
Fragments arranged by size:
2869 1446 742 236 232 191 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 2016 4073 5085 2016
Size: 2057 1012 2944
Fragments arranged by size:
2944 2057 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2641 794
Size: 1847 4166
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 2971 3343 3710 4095 4112 5564 5650 5662
Size: 999 372 367 385 17 1452 86 12
Cuts at: 5662 6008 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 999 385 372 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2220 2617 3073 513
Size: 552 1155 397 456 3453
Fragments arranged by size:
3453 1155 552 456 397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 3529 4323 4343 3529
Size: 794 20 5199
Fragments arranged by size:
5199 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2004 2039 529
Size: 35 1440 35 4503
Fragments arranged by size:
4503 1440 35 35
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DraIII CAC_nnn'GTG
Cuts at: 2927 2927
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2620 3460
Size: 54 1425 50 275 18 354 436 840
Cuts at: 3460 4349 5978 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 840 436 354 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2858 3943 1353
Size: 1505 1085 3423
Fragments arranged by size:
3423 1505 1085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2232 2309 2804 3108 3185 4076
Size: 393 433 167 77 495 304 77 891
Cuts at: 4076 4673 5460 5728 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 891 787 597 495 433 393 304
268 167 77 77
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2102
Size: 6013
EcoO109I rG'GnC_Cy
Cuts at: 2057 2254 2878 2983 3078 3079 3247 3248 2057
Size: 197 624 105 95 1 168 1 4822
Fragments arranged by size:
4822 624 197 168 105 95 1 1
EcoRI G'AATT_C
Cuts at: 3322 3700 3322
Size: 378 5635
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 2476 3139 5160 1678
Size: 798 663 2021 2531
Fragments arranged by size:
2531 2021 798 663
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2370 2402 3281 3313 4654
Size: 32 135 32 798 32 879 32 1341
Cuts at: 4654 4686 4728 4760 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1341 879 798 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3199 3221 3338 3199
Size: 22 117 5874
Fragments arranged by size:
5874 117 22
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5127 5311 1305
Size: 3822 184 2007
Fragments arranged by size:
3822 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2747 3022 3971 4330 4489 518
Size: 648 1581 275 949 359 159 2042
Fragments arranged by size:
2042 1581 949 648 359 275 159
MspA1I CmG'CkG
Cuts at: 1606 2460 2487 3008 3203 3429 4106 5254 5499
Size: 854 27 521 195 226 677 1148 245
Cuts at: 5499 1606
Size: 2120
Fragments arranged by size:
2120 1148 854 677 521 245 226 195
27
NaeI GCC'GGC
Cuts at: 2044 2235 2471 2044
Size: 191 236 5586
Fragments arranged by size:
5586 236 191
NcoI C'CATG_G
Cuts at: 513 1065 2220 3073 513
Size: 552 1155 853 3453
Fragments arranged by size:
3453 1155 853 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NgoMIV G'CCGG_C
Cuts at: 2042 2233 2469 2042
Size: 191 236 5586
Fragments arranged by size:
5586 236 191
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 2365 3371 4916 1132
Size: 97 1136 1006 1545 2229
Fragments arranged by size:
2229 1545 1136 1006 97
PasI CC'CwG_GG
Cuts at: 1348 2035 2947 1348
Size: 687 912 4414
Fragments arranged by size:
4414 912 687
PciI A'CATG_T
Cuts at: 4912 4912
Size: 6013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6013
PfoI T'CCnGG_A
Cuts at: 2156 2817 3114 2156
Size: 661 297 5055
Fragments arranged by size:
5055 661 297
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5595 5627 4082
Size: 32 1481 32 4468
Fragments arranged by size:
4468 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PspOMI G'GGCC_C
Cuts at: 2057 2878 3078 3247 2057
Size: 821 200 169 4823
Fragments arranged by size:
4823 821 200 169
PstI C_TGCA'G
Cuts at: 830 2693 3365 830
Size: 1863 672 3478
Fragments arranged by size:
3478 1863 672
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SexAI A'CCwGG_T
Cuts at: 2923 2923
Size: 6013
SfcI C'TryA_G
Cuts at: 826 1045 2689 3361 4496 5177 5368 826
Size: 219 1644 672 1135 681 191 1471
Fragments arranged by size:
1644 1471 1135 681 672 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 3211 1700
Size: 1511 4502
SmaI CCC'GGG
Cuts at: 1872 2271 2995 3340 1872
Size: 399 724 345 4545
Fragments arranged by size:
4545 724 399 345
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4117 5018 5280 820
Size: 197 926 2174 901 262 1553
Fragments arranged by size:
2174 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 2903 3047 1517
Size: 1386 144 4483
Fragments arranged by size:
4483 1386 144
StyI C'CwwG_G
Cuts at: 513 1065 2220 3073 3241 3452 513
Size: 552 1155 853 168 211 3074
Fragments arranged by size:
3074 1155 853 552 211 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2208 2939 4040 4057 4210 4395 4840
Size: 1255 628 731 1101 17 153 185 445
Cuts at: 4840 325
Size: 1498
Fragments arranged by size:
1498 1255 1101 731 628 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4259 5971 96
Size: 274 80 33 51 1443 2282 1712 138
Fragments arranged by size:
2282 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3222 3254 5973 6005 3222
Size: 32 2719 32 3230
Fragments arranged by size:
3230 2719 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2516 1316
Size: 1200 4813
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 2605
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 2269 2993 3338 1870
Size: 399 724 345 4545
Fragments arranged by size:
4545 724 399 345
XmnI GAAnn'nnTTC
Cuts at: 2312 4142 2312
Size: 1830 4183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI