Restriction map for
pFN21AB7735
(Circular) (Six-base) MAPSORT of: pFN21AB7735.seq Check: 9415 from: 1 to: 6013
pFN21AB7735 6013 bp
With 182 enzymes: SgfI *
March 28, 2012 14:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3057 3356 1937
Size: 1120 299 4594
Fragments arranged by size:
4594 1120 299
Acc65I G'GTAC_C
Cuts at: 3282 3334 3282
Size: 52 5961
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2659 3255 4076 5460 5728 1632
Size: 1027 596 821 1384 268 1917
Fragments arranged by size:
1917 1384 1027 821 596 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 2727 4912 5889 1203
Size: 1524 2185 977 1327
Fragments arranged by size:
2185 1524 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 5981
AlwNI CAG_nnn'CTG
Cuts at: 2335 3756 5328 2335
Size: 1421 1572 3020
Fragments arranged by size:
3020 1572 1421
ApaI G_GGCC'C
Cuts at: 3098 3098
Size: 6013
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 3322 3548 3700 5809 1493
Size: 1829 226 152 2109 1697
Fragments arranged by size:
2109 1829 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 3338 1870
Size: 73 624 771 4545
Fragments arranged by size:
4545 771 624 73
BamHI G'GATC_C
Cuts at: 2474 3343 3710 2474
Size: 869 367 4777
Fragments arranged by size:
4777 869 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2457 3282 3334 4789 618
Size: 325 217 627 670 825 52 1455 1842
Fragments arranged by size:
1842 1455 825 670 627 325 217 52
BanII G_rGCy'C
Cuts at: 1391 2559 3098 3332 1391
Size: 1168 539 234 4072
Fragments arranged by size:
4072 1168 539 234
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5042
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3006 3040 4204 4238 1903
Size: 34 1069 34 1164 34 3678
Fragments arranged by size:
3678 1164 1069 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2335 3905 5115 2335
Size: 1570 1210 3233
Fragments arranged by size:
3233 1570 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2149 2934 6008 2149
Size: 785 3074 2154
Fragments arranged by size:
3074 2154 785
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 2460 2523 3098 4074 5230 1790
Size: 670 63 575 976 1156 2573
Fragments arranged by size:
2573 1156 976 670 575 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6013
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 2436 2510
Size: 416 53 83 399 24 906 113 74
Cuts at: 2510 2553 3744 4702 442
Size: 43 1191 958 1753
Fragments arranged by size:
1753 1191 958 906 416 399 113 83
74 53 43 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2270 2562 2597 2771 2912 3241 4673
Size: 797 234 292 35 174 141 329 1432
Cuts at: 4673 1239
Size: 2579
Fragments arranged by size:
2579 1432 797 329 292 234 174 141
35
Bpu10I CC'TnA_GC
Cuts at: 2714 3190 2714
Size: 476 5537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2723 4138 5003 5301 2723
Size: 1415 865 298 3435
Fragments arranged by size:
3435 1415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2414 2864 3093 4676 882
Size: 617 915 450 229 1583 2219
Fragments arranged by size:
2219 1583 915 617 450 229
BsaAI yAC'GTr
Cuts at: 493 1204 3804 493
Size: 711 2600 2702
Fragments arranged by size:
2702 2600 711
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3001 3820 4202 4837
Size: 53 83 186 936 1468 819 382 635
Cuts at: 4837 275
Size: 1451
Fragments arranged by size:
1468 1451 936 819 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2222 4446 4844 5118 5265 5676 2222
Size: 2224 398 274 147 411 2559
Fragments arranged by size:
2559 2224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2885 2915 3239 3269 1178
Size: 30 165 30 1482 30 324 30 3922
Fragments arranged by size:
3922 1482 324 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2402 2405 2585 2945 1186
Size: 1186 30 3 180 360 4254
Fragments arranged by size:
4254 1186 360 180 30 3
BseYI C'CCAG_C
Cuts at: 2349 2505 2534 2617 2807 2819 2921 5216 5712
Size: 156 29 83 190 12 102 2295 496
Cuts at: 5712 2349
Size: 2650
Fragments arranged by size:
2650 2295 496 190 156 102 83 29
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5440
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3463 4224 5252 1540
Size: 275 186 1462 761 1028 2301
Fragments arranged by size:
2301 1462 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2804 4074 4159 5230 2804
Size: 1270 85 1071 3587
Fragments arranged by size:
3587 1270 1071 85
BsmI GAATG_Cn'
Cuts at: 3524 3617 3524
Size: 93 5920
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2460 2523 2559 2804 3098 3332 4074
Size: 399 670 63 36 245 294 234 742
Cuts at: 4074 4159 5230 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 670 399 294 245 234
85 63 36
BspEI T'CCGG_A
Cuts at: 2222 5676 2222
Size: 3454 2559
BspHI T'CATG_A
Cuts at: 3902 5632 3902
Size: 1730 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2218 3368 844
Size: 1374 1150 3489
Fragments arranged by size:
3489 1374 1150
BsrBI CCG'CTC
Cuts at: 1394 1823 2284 3900 1394
Size: 429 461 1616 3507
Fragments arranged by size:
3507 1616 461 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4508 4682 65
Size: 1183 3260 174 1396
Fragments arranged by size:
3260 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4657 4844 1700
Size: 110 2847 187 2869
Fragments arranged by size:
2869 2847 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2296 2368 794
Size: 1502 72 4439
Fragments arranged by size:
4439 1502 72
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstXI CCAn_nnnn'nTGG
Cuts at: 2237 2453 2237
Size: 216 5797
BstYI r'GATC_y
Cuts at: 1972 2149 2474 2934 3275 3343 3710 4095 4112
Size: 177 325 460 341 68 367 385 17
Cuts at: 4112 5564 5650 5662 6008 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 460 385 367 346 341 325
177 86 68 17 12
Bsu36I CC'TnA_GG
Cuts at: 3082 3082
Size: 6013
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 2961 513
Size: 552 939 60 897 3565
Fragments arranged by size:
3565 939 897 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2663 487
Size: 683 56 1437 3837
Fragments arranged by size:
3837 1437 683 56
BtsI GCAGTG_nn'
Cuts at: 3213 3249 3529 4323 4343 3213
Size: 36 280 794 20 4883
Fragments arranged by size:
4883 794 280 36 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2026 2061 529
Size: 35 1462 35 4481
Fragments arranged by size:
4481 1462 35 35
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2186 2308 3033
Size: 54 1425 50 275 18 356 122 725
Cuts at: 3033 3460 4349 5978 8
Size: 427 889 1629 43
Fragments arranged by size:
1629 1425 889 725 427 356 275 122
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2840 2891 3274 3943 1353
Size: 1487 51 383 669 3423
Fragments arranged by size:
3423 1487 669 383 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2870 3241 4600 4974 5120 387
Size: 1064 138 1281 371 1359 374 146 1280
Fragments arranged by size:
1359 1281 1280 1064 374 371 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2270 2562 2597 2659 2771 2912
Size: 393 404 234 292 35 62 112 141
Cuts at: 2912 3241 3255 4076 4673 5460 5728 1239
Size: 329 14 821 597 787 268 1524
Fragments arranged by size:
1524 821 787 597 404 393 329 292
268 234 141 112 62 35 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3189 3189
Size: 6013
EcoO109I rG'GnC_Cy
Cuts at: 2207 2704 3095 2207
Size: 497 391 5125
Fragments arranged by size:
5125 497 391
EcoRI G'AATT_C
Cuts at: 3322 3700 3322
Size: 378 5635
EcoRV GAT'ATC
Cuts at: 2035 2035
Size: 6013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 6013
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 5160 1678
Size: 3482 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2759 2791 2942 2974 4654
Size: 32 135 32 1187 32 151 32 1680
Cuts at: 4654 4686 4728 4760 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1680 1187 151 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3286 3338 3286
Size: 52 5961
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5127 5311 1305
Size: 3822 184 2007
Fragments arranged by size:
3822 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3035 10
Size: 54 1425 343 1203 2988
Fragments arranged by size:
2988 1425 1203 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3971 4330 4489 518
Size: 648 2805 359 159 2042
Fragments arranged by size:
2805 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3429 4106 5254 5499 1606
Size: 1823 677 1148 245 2120
Fragments arranged by size:
2120 1823 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2961 513
Size: 552 939 957 3565
Fragments arranged by size:
3565 957 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 2731 3371 4916 1132
Size: 97 1502 640 1545 2229
Fragments arranged by size:
2229 1545 1502 640 97
PasI CC'CwG_GG
Cuts at: 1348 2462 3158 1348
Size: 1114 696 4203
Fragments arranged by size:
4203 1114 696
PciI A'CATG_T
Cuts at: 2727 4912 2727
Size: 2185 3828
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2426 1108
Size: 1318 4695
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 4082 4114 5595 5627 3239
Size: 32 811 32 1481 32 3625
Fragments arranged by size:
3625 1481 811 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2207 2207
Size: 6013
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PspOMI G'GGCC_C
Cuts at: 3094 3094
Size: 6013
PspXI vC'TCGA_Gb
Cuts at: 2567 2567
Size: 6013
PstI C_TGCA'G
Cuts at: 830 2169 2373 3365 830
Size: 1339 204 992 3478
Fragments arranged by size:
3478 1339 992 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SfcI C'TryA_G
Cuts at: 826 1045 2165 2369 3361 4496 5177 5368 826
Size: 219 1120 204 992 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 992 681 219 204 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2567 2738 4117 5018 5280 820
Size: 197 926 624 171 1379 901 262 1553
Fragments arranged by size:
1553 1379 926 901 624 262 197 171
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 3175 1517
Size: 1658 4355
StyI C'CwwG_G
Cuts at: 513 1065 2004 2961 3452 513
Size: 552 939 957 491 3074
Fragments arranged by size:
3074 957 939 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3055 4040 4057 4210 4395 4840 325
Size: 1255 1475 985 17 153 185 445 1498
Fragments arranged by size:
1498 1475 1255 985 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4259 5971 96
Size: 274 80 33 51 1443 2282 1712 138
Fragments arranged by size:
2282 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 2567 1943
Size: 624 5389
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 4142 4142
Size: 6013
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI