Restriction map for
pFN21AB7733
(Circular) (Six-base) MAPSORT of: pFN21AB7733.seq Check: 9087 from: 1 to: 6004
pFN21AB7733 6004 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2401 2401
Size: 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2715 3118 4067 5451 5719 1632
Size: 1083 403 949 1384 268 1917
Fragments arranged by size:
1917 1384 1083 949 403 268
AflII C'TTAA_G
Cuts at: 820 1017 2452 820
Size: 197 1435 4372
Fragments arranged by size:
4372 1435 197
AflIII A'CryG_T
Cuts at: 1203 2894 3194 4903 5880 1203
Size: 1691 300 1709 977 1327
Fragments arranged by size:
1709 1691 1327 977 300
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 2085
Size: 32 5972
AlwNI CAG_nnn'CTG
Cuts at: 3747 5319 3747
Size: 1572 4432
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 2068 2887 3313 3539 3691 5800 1493
Size: 575 819 426 226 152 2109 1697
Fragments arranged by size:
2109 1697 819 575 426 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2613 3032 3329 1870
Size: 73 670 419 297 4545
Fragments arranged by size:
4545 670 419 297 73
AvrII C'CTAG_G
Cuts at: 2687 2687
Size: 6004
BamHI G'GATC_C
Cuts at: 2915 3334 3701 2915
Size: 419 367 5218
Fragments arranged by size:
5218 419 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3325 4780 618
Size: 325 217 627 1538 1455 1842
Fragments arranged by size:
1842 1538 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3323 1391
Size: 1932 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2370 3007 928
Size: 971 471 637 3925
Fragments arranged by size:
3925 971 637 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4615 136
Size: 107 122 71 631 605 2943 1525
Fragments arranged by size:
2943 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2014 2759 5999 2014
Size: 745 3240 2019
Fragments arranged by size:
3240 2019 745
BlpI GC'TnA_GC
Cuts at: 3421 3421
Size: 6004
Bme1580I G_kGCm'C
Cuts at: 1790 4065 5221 1790
Size: 2275 1156 2573
Fragments arranged by size:
2573 2275 1156
BmgBI CAC'GTC
Cuts at: 1318 2407 1318
Size: 1089 4915
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3735 4693 442
Size: 416 53 83 399 24 2318 958 1753
Fragments arranged by size:
2318 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 4664 1239
Size: 1052 2373 2579
Fragments arranged by size:
2579 2373 1052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2116 4129 4994 5292 2116
Size: 2013 865 298 2828
Fragments arranged by size:
2828 2013 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3162 4667 882
Size: 617 1663 1505 2219
Fragments arranged by size:
2219 1663 1505 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4437 4835 5109 5256 5667 4437
Size: 398 274 147 411 4774
Fragments arranged by size:
4774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5779
Fragments arranged by size:
5779 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6004
BseYI C'CCAG_C
Cuts at: 5207 5703 5207
Size: 496 5508
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5431
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 4065 4150 5221 2145
Size: 1920 85 1071 2928
Fragments arranged by size:
2928 1920 1071 85
BsmI GAATG_Cn'
Cuts at: 2494 2957 3515 3608 2494
Size: 463 558 93 4890
Fragments arranged by size:
4890 558 463 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 3323 4065 4150 5221 1391
Size: 399 355 1178 742 85 1071 2174
Fragments arranged by size:
2174 1178 1071 742 399 355 85
BspEI T'CCGG_A
Cuts at: 5667 5667
Size: 6004
BspHI T'CATG_A
Cuts at: 3160 3256 3893 5623 3160
Size: 96 637 1730 3541
Fragments arranged by size:
3541 1730 637 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2104 2401 3159 3359 844
Size: 1260 297 758 200 3489
Fragments arranged by size:
3489 1260 758 297 200
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2305 2884 4499 4673 65
Size: 1183 1057 579 1615 174 1396
Fragments arranged by size:
1615 1396 1183 1057 579 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2188 794
Size: 1394 4610
BstBI TT'CG_AA
Cuts at: 2603 4808 2603
Size: 2205 3799
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 2014 2205 2759 2915 3334 3701 4086 4103
Size: 42 191 554 156 419 367 385 17
Cuts at: 4103 5555 5641 5653 5999 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 554 419 385 367 346 191
156 86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2920 513
Size: 552 939 916 3597
Fragments arranged by size:
3597 939 916 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 2751 3520 4314 4334 2293
Size: 458 769 794 20 3963
Fragments arranged by size:
3963 794 769 458 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2290 3451 4340
Size: 54 1425 50 275 18 460 1161 889
Cuts at: 4340 5969 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1161 889 460 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2670 2691 3934 1353
Size: 1317 21 1243 3423
Fragments arranged by size:
3423 1317 1243 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2715 3118 4067 4664 5451 5719
Size: 393 659 424 403 949 597 787 268
Cuts at: 5719 1239
Size: 1524
Fragments arranged by size:
1524 949 787 659 597 424 403 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2093 2093
Size: 6004
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
EcoRV GAT'ATC
Cuts at: 3060 3060
Size: 6004
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4645 4677 4719 4751 1373
Size: 32 135 32 3073 32 42 32 2626
Fragments arranged by size:
3073 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3297 748
Size: 2549 3455
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5118 5302 1305
Size: 3813 184 2007
Fragments arranged by size:
3813 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2292 10
Size: 54 1425 343 460 3722
Fragments arranged by size:
3722 1425 460 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2298 2941 3009 3962 4321 4480 518
Size: 648 1132 643 68 953 359 159 2042
Fragments arranged by size:
2042 1132 953 648 643 359 159 68
MspA1I CmG'CkG
Cuts at: 1606 3420 4097 5245 5490 1606
Size: 1814 677 1148 245 2120
Fragments arranged by size:
2120 1814 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2920 513
Size: 552 939 916 3597
Fragments arranged by size:
3597 939 916 552
NdeI CA'TA_TG
Cuts at: 387 2173 3246 387
Size: 1786 1073 3145
Fragments arranged by size:
3145 1786 1073
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2416 2898 3114 3121 3198 3362 4907
Size: 97 1187 482 216 7 77 164 1545
Cuts at: 4907 1132
Size: 2229
Fragments arranged by size:
2229 1545 1187 482 216 164 97 77
7
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 2894 3194 4903 2894
Size: 300 1709 3995
Fragments arranged by size:
3995 1709 300
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6004
PfoI T'CCnGG_A
Cuts at: 2268 2670 2268
Size: 402 5602
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2093 2093
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PspXI vC'TCGA_Gb
Cuts at: 2613 2613
Size: 6004
PstI C_TGCA'G
Cuts at: 830 3356 830
Size: 2526 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SfcI C'TryA_G
Cuts at: 826 1045 3352 4487 5168 5359 826
Size: 219 2307 1135 681 191 1471
Fragments arranged by size:
2307 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2131 2452 2613 4108 5009 5271
Size: 197 926 188 321 161 1495 901 262
Cuts at: 5271 820
Size: 1553
Fragments arranged by size:
1553 1495 926 901 321 262 197 188
161
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 2629 3928 5
Size: 47 2577 1299 2081
Fragments arranged by size:
2577 2081 1299 47
StuI AGG'CCT
Cuts at: 1517 2692 1517
Size: 1175 4829
StyI C'CwwG_G
Cuts at: 513 1065 2004 2089 2687 2794 2920 2958 3443
Size: 552 939 85 598 107 126 38 485
Cuts at: 3443 513
Size: 3074
Fragments arranged by size:
3074 939 598 552 485 126 107 85
38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4250 5962 96
Size: 274 80 33 51 1443 2273 1712 138
Fragments arranged by size:
2273 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2320 2352 5964 5996 2320
Size: 32 3612 32 2328
Fragments arranged by size:
3612 2328 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 2613 1943
Size: 670 5334
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 3104 4133 3104
Size: 1029 4975
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI