Restriction map for
pFN21AB7732
(Circular) (Six-base) MAPSORT of: pFN21AB7732.seq Check: 8535 from: 1 to: 6151
pFN21AB7732 6151 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3318 3494 1937
Size: 1381 176 4594
Fragments arranged by size:
4594 1381 176
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2110 2458 2710 2813 3046 4214 5598 5866
Size: 478 348 252 103 233 1168 1384 268
Cuts at: 5866 1632
Size: 1917
Fragments arranged by size:
1917 1384 1168 478 348 268 252 233
103
AfeI AGC'GCT
Cuts at: 3395 3395
Size: 6151
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 2625 5050 6027 1203
Size: 1422 2425 977 1327
Fragments arranged by size:
2425 1422 1327 977
AgeI A'CCGG_T
Cuts at: 3321 4982 3321
Size: 1661 4490
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AlwNI CAG_nnn'CTG
Cuts at: 3894 5466 3894
Size: 1572 4579
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2233 2639 2844 3460 3686 3838 5947 1493
Size: 740 406 205 616 226 152 2109 1697
Fragments arranged by size:
2109 1697 740 616 406 226 205 152
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 3476 1870
Size: 73 1533 4545
Fragments arranged by size:
4545 1533 73
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3472 4927 618
Size: 325 217 627 1685 1455 1842
Fragments arranged by size:
1842 1685 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2369 3184 3470 1391
Size: 978 815 286 4072
Fragments arranged by size:
4072 978 815 286
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 2270 3387 928
Size: 971 145 226 1117 3692
Fragments arranged by size:
3692 1117 971 226 145
BbvCI CC'TCA_GC
Cuts at: 2502 2502
Size: 6151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4762 136
Size: 107 122 71 631 605 3090 1525
Fragments arranged by size:
3090 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2310 6146 2310
Size: 3836 2315
BlpI GC'TnA_GC
Cuts at: 2515 3568 2515
Size: 1053 5098
Bme1580I G_kGCm'C
Cuts at: 1790 4212 5368 1790
Size: 2422 1156 2573
Fragments arranged by size:
2573 2422 1156
BmgBI CAC'GTC
Cuts at: 1318 2626 1318
Size: 1308 4843
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2967 3882 4840
Size: 416 53 83 399 24 1550 915 958
Cuts at: 4840 442
Size: 1753
Fragments arranged by size:
1753 1550 958 915 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2627
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2706 3126 4811 1239
Size: 899 568 420 1685 2579
Fragments arranged by size:
2579 1685 899 568 420
Bpu10I CC'TnA_GC
Cuts at: 2502 2502
Size: 6151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2331 3226 4276 5141 5439 2331
Size: 895 1050 865 298 3043
Fragments arranged by size:
3043 1050 895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3190 4814 882
Size: 617 1691 1624 2219
Fragments arranged by size:
2219 1691 1624 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 2734 6145 2734
Size: 3411 2740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3958 4340 4975 275
Size: 53 83 186 936 2425 382 635 1451
Fragments arranged by size:
2425 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3321 4584 4982 5256 5403 5814 3321
Size: 1263 398 274 147 411 3658
Fragments arranged by size:
3658 1263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 1178
Size: 30 165 30 777 30 5119
Fragments arranged by size:
5119 777 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2078 1186
Size: 889 3 5259
Fragments arranged by size:
5259 889 3
BseYI C'CCAG_C
Cuts at: 2068 5354 5850 2068
Size: 3286 496 2369
Fragments arranged by size:
3286 2369 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5578
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2369 4212 4297 5368 2369
Size: 1843 85 1071 3152
Fragments arranged by size:
3152 1843 1071 85
BsmI GAATG_Cn'
Cuts at: 3662 3755 3662
Size: 93 6058
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 3184 3470 4212 4297 5368 1391
Size: 399 579 815 286 742 85 1071 2174
Fragments arranged by size:
2174 1071 815 742 579 399 286 85
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 2815 4040 5770 2815
Size: 1225 1730 3196
Fragments arranged by size:
3196 1730 1225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2142 3506 844
Size: 1298 1364 3489
Fragments arranged by size:
3489 1364 1298
BsrBI CCG'CTC
Cuts at: 1394 1823 4038 1394
Size: 429 2215 3507
Fragments arranged by size:
3507 2215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2752 3268 4646 4820 65
Size: 1183 1504 516 1378 174 1396
Fragments arranged by size:
1504 1396 1378 1183 516 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3321 4795 4982 1700
Size: 110 1511 1474 187 2869
Fragments arranged by size:
2869 1511 1474 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6151
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstYI r'GATC_y
Cuts at: 1972 2247 2310 3481 3848 4233 4250 5702 5788
Size: 275 63 1171 367 385 17 1452 86
Cuts at: 5788 5800 6146 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1171 385 367 346 275 86
63 17 12
BstZ17I GTA'TAC
Cuts at: 3319 3319
Size: 6151
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5599
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 2052 3667 4461 4481 2031
Size: 21 1615 794 20 3701
Fragments arranged by size:
3701 1615 794 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3441 3455 3441
Size: 14 6137
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116
Size: 54 1425 50 275 18 1768 889 1629
Cuts at: 6116 8
Size: 43
Fragments arranged by size:
1768 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3050 3134 3218 4081 1353
Size: 1697 84 84 863 3423
Fragments arranged by size:
3423 1697 863 84 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4738 5112 5258 387
Size: 1064 138 3149 374 146 1280
Fragments arranged by size:
3149 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2367 2367
Size: 6151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2138 2458 2706 2710 2813 3046
Size: 393 478 28 320 248 4 103 233
Cuts at: 3046 3126 4214 4811 5598 5866 1239
Size: 80 1088 597 787 268 1524
Fragments arranged by size:
1524 1088 787 597 478 393 320 268
248 233 103 80 28 4
EcoO109I rG'GnC_Cy
Cuts at: 2805 2805
Size: 6151
EcoRI G'AATT_C
Cuts at: 2233 2639 2844 3460 3838 2233
Size: 406 205 616 378 4546
Fragments arranged by size:
4546 616 406 378 205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3424 3424
Size: 6151
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 3397 3409 5298 1678
Size: 1719 12 1889 2531
Fragments arranged by size:
2531 1889 1719 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2264 2381 2413 2641
Size: 32 135 32 660 32 117 32 228
Cuts at: 2641 2673 2881 2913 4792 4824 4866 4898 1373
Size: 32 208 32 1879 32 42 32 2626
Fragments arranged by size:
2626 1879 660 228 208 135 117 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3741 669
Size: 988 281 1557 246 3079
Fragments arranged by size:
3079 1557 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2554 3311 748
Size: 1806 757 3588
Fragments arranged by size:
3588 1806 757
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 2449 3750 2449
Size: 1301 4850
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2275 5265 5449 1305
Size: 970 2990 184 2007
Fragments arranged by size:
2990 2007 970 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4329
Fragments arranged by size:
4329 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2535 2869 3121 3289 3373 4109
Size: 648 843 526 334 252 168 84 736
Cuts at: 4109 4468 4627 518
Size: 359 159 2042
Fragments arranged by size:
2042 843 736 648 526 359 334 252
168 159 84
MspA1I CmG'CkG
Cuts at: 1606 3567 4244 5392 5637 1606
Size: 1961 677 1148 245 2120
Fragments arranged by size:
2120 1961 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5599
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2799 3509 5054 1132
Size: 97 1570 710 1545 2229
Fragments arranged by size:
2229 1570 1545 710 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6151
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 2784 3204 3721 2784
Size: 420 517 5214
Fragments arranged by size:
5214 517 420
PstI C_TGCA'G
Cuts at: 830 3503 830
Size: 2673 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
SacI G_AGCT'C
Cuts at: 2369 2369
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SapI GCTCTTCn'nnn_
Cuts at: 3050 3134 3218 3050
Size: 84 84 5983
Fragments arranged by size:
5983 84 84
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SfcI C'TryA_G
Cuts at: 826 1045 3499 4634 5315 5506 826
Size: 219 2454 1135 681 191 1471
Fragments arranged by size:
2454 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2346 3241 4255 5156 5418 820
Size: 197 926 403 895 1014 901 262 1553
Fragments arranged by size:
1553 1014 926 901 895 403 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 2344 3239 1517
Size: 827 895 4429
Fragments arranged by size:
4429 895 827
StyI C'CwwG_G
Cuts at: 513 1065 2016 3590 513
Size: 552 951 1574 3074
Fragments arranged by size:
3074 1574 951 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4178 4195 4348 4533 4978 325
Size: 1255 2598 17 153 185 445 1498
Fragments arranged by size:
2598 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4397 6109 96
Size: 274 80 33 51 1443 2420 1712 138
Fragments arranged by size:
2420 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6111 6143 6111
Size: 32 6119
Tth111I GACn'n_nGTC
Cuts at: 1316 2011 1316
Size: 695 5456
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2292 2292
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 2696 2864 3059 4280 2696
Size: 168 195 1221 4567
Fragments arranged by size:
4567 1221 195 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI