Restriction map for
pFN21AB7731
(Circular) (Six-base) MAPSORT of: pFN21AB7731.seq Check: 4782 from: 1 to: 6001
pFN21AB7731 6001 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3344 1937
Size: 1407 4594
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4064 5448 5716 1632
Size: 2432 1384 268 1917
Fragments arranged by size:
2432 1917 1384 268
AfeI AGC'GCT
Cuts at: 2987 2987
Size: 6001
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2079 2291 2987 1184
Size: 895 212 696 4198
Fragments arranged by size:
4198 895 696 212
AlwNI CAG_nnn'CTG
Cuts at: 2032 2201 2967 3744 5316 2032
Size: 169 766 777 1572 2717
Fragments arranged by size:
2717 1572 777 766 169
ApaI G_GGCC'C
Cuts at: 2445 2445
Size: 6001
ApaLI G'TGCA_C
Cuts at: 2047 4058 5214 2047
Size: 2011 1156 2834
Fragments arranged by size:
2834 2011 1156
ApoI r'AATT_y
Cuts at: 1493 2639 3310 3536 3688 5797 1493
Size: 1146 671 226 152 2109 1697
Fragments arranged by size:
2109 1697 1146 671 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3326 1870
Size: 73 1383 4545
Fragments arranged by size:
4545 1383 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2828 2953 3103 3322 4777
Size: 325 217 627 1041 125 150 219 1455
Cuts at: 4777 618
Size: 1842
Fragments arranged by size:
1842 1455 1041 627 325 219 217 150
125
BanII G_rGCy'C
Cuts at: 1391 2222 2239 2445 2570 3012 3320 1391
Size: 831 17 206 125 442 308 4072
Fragments arranged by size:
4072 831 442 308 206 125 17
BbeI G_GCGC'C
Cuts at: 2832 2957 3107 2832
Size: 125 150 5726
Fragments arranged by size:
5726 150 125
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3115 928
Size: 971 1216 3814
Fragments arranged by size:
3814 1216 971
BbvCI CC'TCA_GC
Cuts at: 2461 2461
Size: 6001
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3073 3107 4192 4226 1903
Size: 34 1136 34 1085 34 3678
Fragments arranged by size:
3678 1136 1085 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2574 2951 4612
Size: 107 122 71 631 605 902 377 1661
Cuts at: 4612 136
Size: 1525
Fragments arranged by size:
1661 1525 902 631 605 377 122 107
71
BglII A'GATC_T
Cuts at: 2481 2721 3238 5996 2481
Size: 240 517 2758 2486
Fragments arranged by size:
2758 2486 517 240
BlpI GC'TnA_GC
Cuts at: 3418 3418
Size: 6001
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2288 2445 2874 4062 5218 1790
Size: 261 237 157 429 1188 1156 2573
Fragments arranged by size:
2573 1188 1156 429 261 237 157
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2298 3732 4690
Size: 416 53 83 399 24 881 1434 958
Cuts at: 4690 442
Size: 1753
Fragments arranged by size:
1753 1434 958 881 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2796 1056
Size: 1740 4261
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2276 2276
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2427 4661 1239
Size: 815 373 2234 2579
Fragments arranged by size:
2579 2234 815 373
Bpu10I CC'TnA_GC
Cuts at: 2461 2805 2461
Size: 344 5657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4126 4991 5289 4126
Size: 865 298 4838
Fragments arranged by size:
4838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4664 882
Size: 617 3165 2219
Fragments arranged by size:
3165 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2842 3076 3792 493
Size: 711 1638 234 716 2702
Fragments arranged by size:
2702 1638 716 711 234
BsaBI GATnn'nnATC
Cuts at: 2720 5995 2720
Size: 3275 2726
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2829 2954 3104 3808
Size: 53 83 186 936 1296 125 150 704
Cuts at: 3808 4190 4825 275
Size: 382 635 1451
Fragments arranged by size:
1451 1296 936 704 635 382 186 150
125 83 53
BsaWI w'CCGG_w
Cuts at: 4434 4832 5106 5253 5664 4434
Size: 398 274 147 411 4771
Fragments arranged by size:
4771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2400 2430 1178
Size: 30 165 30 997 30 4749
Fragments arranged by size:
4749 997 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2355 2449 1186
Size: 1169 94 4738
Fragments arranged by size:
4738 1169 94
BseYI C'CCAG_C
Cuts at: 2239 2578 5204 5700 2239
Size: 339 2626 496 2540
Fragments arranged by size:
2626 2540 496 339
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2765 1163
Size: 573 1029 4399
Fragments arranged by size:
4399 1029 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2282 2669 3451 4212 5240 1540
Size: 275 186 281 387 782 761 1028 2301
Fragments arranged by size:
2301 1028 782 761 387 281 275 186
BsiHKAI G_wGCw'C
Cuts at: 2051 2222 4062 4147 5218 2051
Size: 171 1840 85 1071 2834
Fragments arranged by size:
2834 1840 1071 171 85
BsmI GAATG_Cn'
Cuts at: 3512 3605 3512
Size: 93 5908
BsmBI CGTCTCn'nnnn_
Cuts at: 3264 3264
Size: 6001
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2222 2239 2288 2445 2570 2874
Size: 399 261 171 17 49 157 125 304
Cuts at: 2874 3012 3320 4062 4147 5218 1391
Size: 138 308 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 308 304 261 171
157 138 125 85 49 17
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2700 3063 3356 844
Size: 1856 363 293 3489
Fragments arranged by size:
3489 1856 363 293
BsrBI CCG'CTC
Cuts at: 1394 1823 2821 3888 1394
Size: 429 998 1067 3507
Fragments arranged by size:
3507 1067 998 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4496 4670 65
Size: 1183 3248 174 1396
Fragments arranged by size:
3248 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2664 2736 2788 4645 4832 1700
Size: 110 854 72 52 1857 187 2869
Fragments arranged by size:
2869 1857 854 187 110 72 52
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 2613 1925
Size: 688 5313
BssSI C'ACGA_G
Cuts at: 2608 4061 5073 2608
Size: 1453 1012 3536
Fragments arranged by size:
3536 1453 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6001
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2327 3206 2179
Size: 148 879 4974
Fragments arranged by size:
4974 879 148
BstYI r'GATC_y
Cuts at: 1972 2481 2721 3238 3331 3698 4083 4100 5552
Size: 509 240 517 93 367 385 17 1452
Cuts at: 5552 5638 5650 5996 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 517 509 385 367 346 240
93 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3018 3099 3205 513
Size: 552 1953 81 106 3309
Fragments arranged by size:
3309 1953 552 106 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 2538 3517 4311 4331 2538
Size: 979 794 20 4208
Fragments arranged by size:
4208 979 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2279 2666 2685
Size: 54 1425 50 275 18 449 387 19
Cuts at: 2685 2836 2854 2940 3156 3448 4337 5966 8
Size: 151 18 86 216 292 889 1629 43
Fragments arranged by size:
1629 1425 889 449 387 292 275 216
151 86 54 50 43 19 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2279 2666 3448 1537
Size: 275 467 387 782 4090
Fragments arranged by size:
4090 782 467 387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3931 1353
Size: 2578 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4588 4962 5108 387
Size: 1064 138 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2220 2220
Size: 6001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2427 4064 4661 5448 5716 1239
Size: 393 422 373 1637 597 787 268 1524
Fragments arranged by size:
1637 1524 787 597 422 393 373 268
EcoO109I rG'GnC_Cy
Cuts at: 2029 2029
Size: 6001
EcoRI G'AATT_C
Cuts at: 2639 3310 3688 2639
Size: 671 378 4952
Fragments arranged by size:
4952 671 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 6001
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 2832 2957 2989 2999 3051 3107 5148 1678
Size: 1154 125 32 10 52 56 2041 2531
Fragments arranged by size:
2531 2041 1154 125 56 52 32 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2879 2911 4642 4674 4716
Size: 32 135 32 1307 32 1731 32 42
Cuts at: 4716 4748 1373
Size: 32 2626
Fragments arranged by size:
2626 1731 1307 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KasI G'GCGC_C
Cuts at: 2828 2953 3103 2828
Size: 125 150 5726
Fragments arranged by size:
5726 150 125
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2687 2838 10
Size: 54 1425 343 855 151 3173
Fragments arranged by size:
3173 1425 855 343 151 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3204 3959 4318 4477 518
Size: 648 2038 755 359 159 2042
Fragments arranged by size:
2042 2038 755 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2271 2532 2620 2834 2860 2967 3417 4094
Size: 665 261 88 214 26 107 450 677
Cuts at: 4094 5242 5487 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 665 450 261 245 214
107 88 26
NaeI GCC'GGC
Cuts at: 2666 2790 2666
Size: 124 5877
NarI GG'CG_CC
Cuts at: 2829 2954 3104 2829
Size: 125 150 5726
Fragments arranged by size:
5726 150 125
NcoI C'CATG_G
Cuts at: 513 1065 3018 3099 3205 513
Size: 552 1953 81 106 3309
Fragments arranged by size:
3309 1953 552 106 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NgoMIV G'CCGG_C
Cuts at: 2664 2788 2664
Size: 124 5877
NheI G'CTAG_C
Cuts at: 1052 2792 1052
Size: 1740 4261
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 2278 3359 4904 1132
Size: 97 1049 1081 1545 2229
Fragments arranged by size:
2229 1545 1081 1049 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3019 1108
Size: 1911 4090
PfoI T'CCnGG_A
Cuts at: 2404 2404
Size: 6001
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PmlI CAC'GTG
Cuts at: 2842 2842
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PspOMI G'GGCC_C
Cuts at: 2441 2441
Size: 6001
PstI C_TGCA'G
Cuts at: 830 2545 3060 3353 830
Size: 1715 515 293 3478
Fragments arranged by size:
3478 1715 515 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2271 2532 2620 2860 2967 2271
Size: 261 88 240 107 5305
Fragments arranged by size:
5305 261 240 107 88
SacI G_AGCT'C
Cuts at: 2222 2222
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SbfI CC_TGCA'GG
Cuts at: 3060 3353 3060
Size: 293 5708
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SfcI C'TryA_G
Cuts at: 826 1045 2104 2131 2541 3056 3169 3349 4484
Size: 219 1059 27 410 515 113 180 1135
Cuts at: 4484 5165 5356 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1059 681 515 410 219 191
180 113 27
SfoI GGC'GCC
Cuts at: 2830 2955 3105 2830
Size: 125 150 5726
Fragments arranged by size:
5726 150 125
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4105 5006 5268 820
Size: 197 926 2162 901 262 1553
Fragments arranged by size:
2162 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 2278 3359 1132
Size: 1146 1081 3774
Fragments arranged by size:
3774 1146 1081
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2769 3018 3099 3205 3440 513
Size: 552 1704 249 81 106 235 3074
Fragments arranged by size:
3074 1704 552 249 235 106 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2410 4028 4045 4198 4383 4828 325
Size: 1255 830 1618 17 153 185 445 1498
Fragments arranged by size:
1618 1498 1255 830 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2748 4247 5959
Size: 274 80 33 51 1443 771 1499 1712
Cuts at: 5959 96
Size: 138
Fragments arranged by size:
1712 1499 1443 771 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2176 2208 2400 2432 5961 5993 2176
Size: 32 192 32 3529 32 2184
Fragments arranged by size:
3529 2184 192 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2306 2306
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 4130 4130
Size: 6001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI