Restriction map for
pFN21AB7730
(Circular) (Six-base) MAPSORT of: pFN21AB7730.seq Check: 1268 from: 1 to: 6145
pFN21AB7730 6145 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2145 3955 278
Size: 53 83 186 936 609 1810 2468
Fragments arranged by size:
2468 1810 936 609 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3271 3325 4208 5592 5860 1632
Size: 1639 54 883 1384 268 1917
Fragments arranged by size:
1917 1639 1384 883 268 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 5044 6021 1203
Size: 3841 977 1327
Fragments arranged by size:
3841 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2705 1184
Size: 1521 4624
AlwNI CAG_nnn'CTG
Cuts at: 2122 2396 2435 3888 5460 2122
Size: 274 39 1453 1572 2807
Fragments arranged by size:
2807 1572 1453 274 39
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 3454 3680 3832 5941 1493
Size: 1961 226 152 2109 1697
Fragments arranged by size:
2109 1961 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2018 2083 2281 2613 3470 1870
Size: 73 75 65 198 332 857 4545
Fragments arranged by size:
4545 857 332 198 75 73 65
BamHI G'GATC_C
Cuts at: 3180 3475 3842 3180
Size: 295 367 5483
Fragments arranged by size:
5483 367 295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2714 2933 3041 3084
Size: 325 217 627 227 700 219 108 43
Cuts at: 3084 3316 3466 4921 618
Size: 232 150 1455 1842
Fragments arranged by size:
1842 1455 700 627 325 232 227 219
217 150 108 43
BanII G_rGCy'C
Cuts at: 1391 2363 2468 3464 1391
Size: 972 105 996 4072
Fragments arranged by size:
4072 996 972 105
BbeI G_GCGC'C
Cuts at: 2018 3088 2018
Size: 1070 5075
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2137 3022 928
Size: 971 238 885 4051
Fragments arranged by size:
4051 971 885 238
BbvCI CC'TCA_GC
Cuts at: 2389 2389
Size: 6145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4336 4370 1903
Size: 34 2399 34 3678
Fragments arranged by size:
3678 2399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2330 2409 4756
Size: 107 122 71 631 605 658 79 2347
Cuts at: 4756 136
Size: 1525
Fragments arranged by size:
2347 1525 658 631 605 122 107 79
71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 2717 4206 5362 1790
Size: 927 1489 1156 2573
Fragments arranged by size:
2573 1489 1156 927
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3876 4834 442
Size: 416 53 83 399 24 2459 958 1753
Fragments arranged by size:
2459 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2432 2432
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2112 2418 2427 2565 2815 3376 4805 1239
Size: 873 306 9 138 250 561 1429 2579
Fragments arranged by size:
2579 1429 873 561 306 250 138 9
Bpu10I CC'TnA_GC
Cuts at: 2389 2844 2389
Size: 455 5690
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4270 5135 5433 4270
Size: 865 298 4982
Fragments arranged by size:
4982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3936 493
Size: 711 2732 2702
Fragments arranged by size:
2732 2702 711
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2142 2375 2634
Size: 53 83 186 936 482 127 233 259
Cuts at: 2634 3085 3374 3952 4334 4969 275
Size: 451 289 578 382 635 1451
Fragments arranged by size:
1451 936 635 578 482 451 382 289
259 233 186 127 83 53
BsaWI w'CCGG_w
Cuts at: 2243 3195 3350 4578 4976 5250 5397 5808 2243
Size: 952 155 1228 398 274 147 411 2580
Fragments arranged by size:
2580 1228 952 411 398 274 155 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2489 2519 3374 3404 1178
Size: 30 165 30 1086 30 855 30 3919
Fragments arranged by size:
3919 1086 855 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2631 3263 3379 1186
Size: 1445 632 116 3952
Fragments arranged by size:
3952 1445 632 116
BseYI C'CCAG_C
Cuts at: 2435 2718 5348 5844 2435
Size: 283 2630 496 2736
Fragments arranged by size:
2736 2630 496 283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 2628 3125 3312 1163
Size: 573 630 262 497 187 3996
Fragments arranged by size:
3996 630 573 497 262 187
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3027 3595 4356 5384 1540
Size: 275 186 48 978 568 761 1028 2301
Fragments arranged by size:
2301 1028 978 761 568 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2468 4206 4291 5362 2468
Size: 1738 85 1071 3251
Fragments arranged by size:
3251 1738 1071 85
BsmI GAATG_Cn'
Cuts at: 2062 3656 3749 2062
Size: 1594 93 4458
Fragments arranged by size:
4458 1594 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2218 2583 2218
Size: 365 5780
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2363 2468 2717 3464 4206 4291 5362
Size: 399 573 105 249 747 742 85 1071
Cuts at: 5362 1391
Size: 2174
Fragments arranged by size:
2174 1071 747 742 573 399 249 105
85
BspEI T'CCGG_A
Cuts at: 3350 5808 3350
Size: 2458 3687
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3500 844
Size: 2656 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2195 2448 4032 1394
Size: 429 372 253 1584 3507
Fragments arranged by size:
3507 1584 429 372 253
BsrDI GCAATG_nn'
Cuts at: 65 1248 2959 4640 4814 65
Size: 1183 1711 1681 174 1396
Fragments arranged by size:
1711 1681 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2764 2824 2936 3038 4789 4976 1700
Size: 110 954 60 112 102 1751 187 2869
Fragments arranged by size:
2869 1751 954 187 112 110 102 60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 2207 1925
Size: 282 5863
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2122 794
Size: 1328 4817
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 3430 1184
Size: 2246 3899
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 3180 3475 3842 4227 4244 5696 5782 5794
Size: 1208 295 367 385 17 1452 86 12
Cuts at: 5794 6140 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1208 385 367 346 295 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2565 2595 3438 513
Size: 552 939 561 30 843 3220
Fragments arranged by size:
3220 939 843 561 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5406
Fragments arranged by size:
5406 683 56
BtsI GCAGTG_nn'
Cuts at: 2378 3661 4455 4475 2378
Size: 1283 794 20 4048
Fragments arranged by size:
4048 1283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2253 2288 2941 2976 3103 3138 529
Size: 35 1689 35 653 35 127 35 3536
Fragments arranged by size:
3536 1689 653 127 35 35 35 35
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2938 3024
Size: 54 1425 50 275 18 216 892 86
Cuts at: 3024 3406 3592 4481 6110 8
Size: 382 186 889 1629 43
Fragments arranged by size:
1629 1425 892 889 382 275 216 186
86 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3024 3592 1537
Size: 275 234 978 568 4090
Fragments arranged by size:
4090 978 568 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2801 3028 3215 4075 1353
Size: 1448 227 187 860 3423
Fragments arranged by size:
3423 1448 860 227 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3251 4732 5106 5252 387
Size: 1064 138 1662 1481 374 146 1280
Fragments arranged by size:
1662 1481 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2466 2466
Size: 6145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2418 2427 2565 2815 3271 3325
Size: 393 480 306 9 138 250 456 54
Cuts at: 3325 3376 4208 4805 5592 5860 1239
Size: 51 832 597 787 268 1524
Fragments arranged by size:
1524 832 787 597 480 456 393 306
268 250 138 54 51 9
EcoO109I rG'GnC_Cy
Cuts at: 2402 3103 2402
Size: 701 5444
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FspI TGC'GCA
Cuts at: 2061 4651 2061
Size: 2590 3555
HaeII r_GCGC'y
Cuts at: 1678 2018 3088 5292 1678
Size: 340 1070 2204 2531
Fragments arranged by size:
2531 2204 1070 340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2985 3017 3172 3204 3239
Size: 32 135 32 1413 32 155 32 35
Cuts at: 3239 3263 3271 3295 4786 4818 4860 4892 1373
Size: 24 8 24 1491 32 42 32 2626
Fragments arranged by size:
2626 1491 1413 155 135 42 35 32
32 32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2893 3151 3489 3735 669
Size: 988 281 955 258 338 246 3079
Fragments arranged by size:
3079 988 955 338 281 258 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3151 3735 3151
Size: 584 5561
KasI G'GCGC_C
Cuts at: 2014 3084 2014
Size: 1070 5075
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3231 3395 5259 5443 1305
Size: 1926 164 1864 184 2007
Fragments arranged by size:
2007 1926 1864 184 164
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4323
Fragments arranged by size:
4323 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2669 4103 4462 4621 518
Size: 648 843 660 1434 359 159 2042
Fragments arranged by size:
2042 1434 843 660 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2327 2393 2460 2597 2790 3296 3561
Size: 513 208 66 67 137 193 506 265
Cuts at: 3561 4238 5386 5631 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 513 506 265 245 208
193 137 67 66
NaeI GCC'GGC
Cuts at: 2766 3040 2766
Size: 274 5871
NarI GG'CG_CC
Cuts at: 2015 3085 2015
Size: 1070 5075
NcoI C'CATG_G
Cuts at: 513 1065 2004 3438 513
Size: 552 939 1434 3220
Fragments arranged by size:
3220 1434 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NgoMIV G'CCGG_C
Cuts at: 2764 3038 2764
Size: 274 5871
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 3268 3503 5048 1132
Size: 97 2039 235 1545 2229
Fragments arranged by size:
2229 2039 1545 235 97
PasI CC'CwG_GG
Cuts at: 1348 2429 1348
Size: 1081 5064
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2435 3081 1108
Size: 1327 646 4172
Fragments arranged by size:
4172 1327 646
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3103 3103
Size: 6145
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PstI C_TGCA'G
Cuts at: 830 3497 830
Size: 2667 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2119 2393 3296 2119
Size: 274 903 4968
Fragments arranged by size:
4968 903 274
SacI G_AGCT'C
Cuts at: 2468 2468
Size: 6145
SacII CC_GC'GG
Cuts at: 2598 2598
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SanDI GG'GwC_CC
Cuts at: 3103 3103
Size: 6145
SapI GCTCTTCn'nnn_
Cuts at: 3028 3215 3028
Size: 187 5958
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SexAI A'CCwGG_T
Cuts at: 2643 2643
Size: 6145
SfcI C'TryA_G
Cuts at: 826 1045 2218 2487 2782 3493 4628 5309 5500
Size: 219 1173 269 295 711 1135 681 191
Cuts at: 5500 826
Size: 1471
Fragments arranged by size:
1471 1173 1135 711 681 295 269 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2409 2409
Size: 6145
SfoI GGC'GCC
Cuts at: 2016 3086 2016
Size: 1070 5075
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 2085 2283 3472 1872
Size: 213 198 1189 4545
Fragments arranged by size:
4545 1189 213 198
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4249 5150 5412 820
Size: 197 926 2306 901 262 1553
Fragments arranged by size:
2306 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3268 3503 1132
Size: 2136 235 3774
Fragments arranged by size:
3774 2136 235
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 2700 1517
Size: 1183 4962
StyI C'CwwG_G
Cuts at: 513 1065 2004 3438 3584 513
Size: 552 939 1434 146 3074
Fragments arranged by size:
3074 1434 939 552 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2542 2551 2732 4172 4189 4342 4527
Size: 1255 962 9 181 1440 17 153 185
Cuts at: 4527 4972 325
Size: 445 1498
Fragments arranged by size:
1498 1440 1255 962 445 185 181 153
17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4391 6103 96
Size: 274 80 33 51 1443 2414 1712 138
Fragments arranged by size:
2414 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2489 2521 3374 3406 6105 6137 2489
Size: 32 853 32 2699 32 2497
Fragments arranged by size:
2699 2497 853 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2633 3107 1316
Size: 1317 474 4354
Fragments arranged by size:
4354 1317 474
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2978 2978
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 2083 2281 3470 1870
Size: 213 198 1189 4545
Fragments arranged by size:
4545 1189 213 198
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2143 3953 276
Size: 53 83 186 936 609 1810 2468
Fragments arranged by size:
2468 1810 936 609 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SrfI SwaI