Restriction map for
pFN21AB7729
(Circular) (Six-base) MAPSORT of: pFN21AB7729.seq Check: 3748 from: 1 to: 5998
pFN21AB7729 5998 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 2203 2610 2627 4061 5445 5713 1632
Size: 495 76 407 17 1434 1384 268 1917
Fragments arranged by size:
1917 1434 1384 495 407 268 76 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 3170 3239 4897 5874 1203
Size: 1967 69 1658 977 1327
Fragments arranged by size:
1967 1658 1327 977 69
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 2152 4727 2152
Size: 2575 3423
AleI CACnn'nnGTG
Cuts at: 1166 2141 1166
Size: 975 5023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3173 1184
Size: 1989 4009
AlwNI CAG_nnn'CTG
Cuts at: 2057 2605 3741 5313 2057
Size: 548 1136 1572 2742
Fragments arranged by size:
2742 1572 1136 548
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 2415 2476 2766 2996 3231 3307 3533 3685
Size: 922 61 290 230 235 76 226 152
Cuts at: 3685 5794 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 922 290 235 230 226 152
76 61
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 3323 1870
Size: 73 1380 4545
Fragments arranged by size:
4545 1380 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3051 3084 3051
Size: 33 5965
BamHI G'GATC_C
Cuts at: 3328 3695 3328
Size: 367 5631
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3319 4774 618
Size: 325 217 627 1532 1455 1842
Fragments arranged by size:
1842 1532 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3195 3317 1391
Size: 1804 122 4072
Fragments arranged by size:
4072 1804 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2231 2351 2958 2967 928
Size: 971 332 120 607 9 3959
Fragments arranged by size:
3959 971 607 332 120 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4189 4223 1903
Size: 34 2252 34 3678
Fragments arranged by size:
3678 2252 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BfrBI ATG'CAT
Cuts at: 2834 3107 2834
Size: 273 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4609 136
Size: 107 122 71 631 605 2937 1525
Fragments arranged by size:
2937 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5993 5993
Size: 5998
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 4059 5215 1790
Size: 2269 1156 2573
Fragments arranged by size:
2573 2269 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2841 3729 4687
Size: 416 53 83 399 24 1424 888 958
Cuts at: 4687 442
Size: 1753
Fragments arranged by size:
1753 1424 958 888 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2013 4658 1239
Size: 774 2645 2579
Fragments arranged by size:
2645 2579 774
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4123 4988 5286 4123
Size: 865 298 4835
Fragments arranged by size:
4835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3019 3789 493
Size: 711 1815 770 2702
Fragments arranged by size:
2702 1815 770 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2575 4431 4829 5103 5250 5661 2575
Size: 1856 398 274 147 411 2912
Fragments arranged by size:
2912 1856 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2265 2295 1178
Size: 30 165 30 862 30 4881
Fragments arranged by size:
4881 862 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2833 3203 1186
Size: 1647 370 3981
Fragments arranged by size:
3981 1647 370
BseYI C'CCAG_C
Cuts at: 5201 5697 5201
Size: 496 5502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 1163
Size: 573 706 4719
Fragments arranged by size:
4719 706 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 3195 4059 4144 5215 2385
Size: 810 864 85 1071 3168
Fragments arranged by size:
3168 1071 864 810 85
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 3195 3317 4059 4144 5215 1391
Size: 399 595 810 122 742 85 1071 2174
Fragments arranged by size:
2174 1071 810 742 595 399 122 85
BspEI T'CCGG_A
Cuts at: 2575 5661 2575
Size: 3086 2912
BspHI T'CATG_A
Cuts at: 2191 3887 5617 2191
Size: 1696 1730 2572
Fragments arranged by size:
2572 1730 1696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3353 844
Size: 2509 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2739 2804 2836 4493 4667 65
Size: 1183 1491 65 32 1657 174 1396
Fragments arranged by size:
1657 1491 1396 1183 174 65 32
BsrFI r'CCGG_y
Cuts at: 1700 1810 4642 4829 1700
Size: 110 2832 187 2869
Fragments arranged by size:
2869 2832 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 4058 5070 4058
Size: 1012 4986
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5998
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2469 3227 2143
Size: 326 758 4914
Fragments arranged by size:
4914 758 326
BstYI r'GATC_y
Cuts at: 1972 3209 3328 3695 4080 4097 5549 5635 5647
Size: 1237 119 367 385 17 1452 86 12
Cuts at: 5647 5993 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1237 385 367 346 119 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2533 513
Size: 552 939 529 3978
Fragments arranged by size:
3978 939 552 529
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 3040 3514 4308 4328 3040
Size: 474 794 20 4710
Fragments arranged by size:
4710 794 474 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2872 2907 529
Size: 35 2308 35 3620
Fragments arranged by size:
3620 2308 35 35
DraI TTT'AAA
Cuts at: 2745 3302 2745
Size: 557 5441
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 3445 4334
Size: 54 1425 50 275 18 543 1072 889
Cuts at: 4334 5963 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1072 889 543 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 3928 1353
Size: 1440 1135 3423
Fragments arranged by size:
3423 1440 1135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3193 3193
Size: 5998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2013 2127 2203 2610 2627 4061 4658
Size: 393 381 114 76 407 17 1434 597
Cuts at: 4658 5445 5713 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1434 787 597 407 393 381 268
114 76 17
EcoRI G'AATT_C
Cuts at: 2476 3231 3307 3685 2476
Size: 755 76 378 4789
Fragments arranged by size:
4789 755 378 76
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 5145 1678
Size: 3467 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2138 2170 2270 2302 3201
Size: 32 135 32 566 32 100 32 899
Cuts at: 3201 3233 3262 3294 4639 4671 4713 4745 1373
Size: 32 29 32 1345 32 42 32 2626
Fragments arranged by size:
2626 1345 899 566 135 100 42 32
32 32 32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2098 748
Size: 1350 4648
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 2244 3597 2244
Size: 1353 4645
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5112 5296 1305
Size: 3807 184 2007
Fragments arranged by size:
3807 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 3633
Fragments arranged by size:
3633 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2369 2847 3956 4315 4474 518
Size: 648 975 228 478 1109 359 159 2042
Fragments arranged by size:
2042 1109 975 648 478 359 228 159
MspA1I CmG'CkG
Cuts at: 1606 3206 3414 4091 5239 5484 1606
Size: 1600 208 677 1148 245 2120
Fragments arranged by size:
2120 1600 1148 677 245 208
NcoI C'CATG_G
Cuts at: 513 1065 2004 2533 513
Size: 552 939 529 3978
Fragments arranged by size:
3978 939 552 529
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NsiI A_TGCA'T
Cuts at: 2836 3109 2836
Size: 273 5725
NspI r_CATG'y
Cuts at: 1132 1229 3174 3243 3356 4901 1132
Size: 97 1945 69 113 1545 2229
Fragments arranged by size:
2229 1945 1545 113 97 69
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5998
PciI A'CATG_T
Cuts at: 3170 3239 4897 3170
Size: 69 1658 4271
Fragments arranged by size:
4271 1658 69
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2018 2291 1108
Size: 910 273 4815
Fragments arranged by size:
4815 910 273
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PmlI CAC'GTG
Cuts at: 3019 3019
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PstI C_TGCA'G
Cuts at: 830 3350 830
Size: 2520 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 3206 3206
Size: 5998
SacI G_AGCT'C
Cuts at: 3195 3195
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 3262 4246 5958 3262
Size: 984 1712 3302
Fragments arranged by size:
3302 1712 984
SexAI A'CCwGG_T
Cuts at: 2688 2688
Size: 5998
SfcI C'TryA_G
Cuts at: 826 1045 2754 3346 4481 5162 5353 826
Size: 219 1709 592 1135 681 191 1471
Fragments arranged by size:
1709 1471 1135 681 592 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4102 5003 5265 820
Size: 197 926 2159 901 262 1553
Fragments arranged by size:
2159 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2004 2533 3437 513
Size: 552 939 529 904 3074
Fragments arranged by size:
3074 939 904 552 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4025 4042 4195 4380 4825 325
Size: 1255 2445 17 153 185 445 1498
Fragments arranged by size:
2445 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3260 4244 5956
Size: 274 80 33 51 1443 1283 984 1712
Cuts at: 5956 96
Size: 138
Fragments arranged by size:
1712 1443 1283 984 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2265 2297 5958 5990 2265
Size: 32 3661 32 2273
Fragments arranged by size:
3661 2273 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 2554 3042 4127 2554
Size: 488 1085 4425
Fragments arranged by size:
4425 1085 488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI