Restriction map for
pFN21AB7728
(Circular) (Six-base) MAPSORT of: pFN21AB7728.seq Check: 5869 from: 1 to: 6142
pFN21AB7728 6142 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2634 2925 2634
Size: 291 5851
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2336 3300 3485 1937
Size: 399 964 185 4594
Fragments arranged by size:
4594 964 399 185
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3041 4205 5589 5857 1632
Size: 1409 1164 1384 268 1917
Fragments arranged by size:
1917 1409 1384 1164 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 2586 5041 6018 1203
Size: 1383 2455 977 1327
Fragments arranged by size:
2455 1383 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2078 1184
Size: 894 5248
AlwNI CAG_nnn'CTG
Cuts at: 2353 3011 3139 3885 5457 2353
Size: 658 128 746 1572 3038
Fragments arranged by size:
3038 1572 746 658 128
ApaI G_GGCC'C
Cuts at: 3314 3314
Size: 6142
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 2528 3451 3677 3829 5938 1493
Size: 1035 923 226 152 2109 1697
Fragments arranged by size:
2109 1697 1035 923 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2117 3030 3467 1870
Size: 73 84 90 913 437 4545
Fragments arranged by size:
4545 913 437 90 84 73
BamHI G'GATC_C
Cuts at: 2388 3069 3472 3839 2388
Size: 681 403 367 4691
Fragments arranged by size:
4691 681 403 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3463 4918 618
Size: 325 217 627 1676 1455 1842
Fragments arranged by size:
1842 1676 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3314 3461 1391
Size: 1923 147 4072
Fragments arranged by size:
4072 1923 147
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2305 2693 928
Size: 971 406 388 4377
Fragments arranged by size:
4377 971 406 388
BbvCI CC'TCA_GC
Cuts at: 3383 3383
Size: 6142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4333 4367 1903
Size: 34 50 34 2312 34 3678
Fragments arranged by size:
3678 2312 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3318 4753 136
Size: 107 122 71 631 605 1646 1435 1525
Fragments arranged by size:
1646 1525 1435 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2272 3048 6137 2272
Size: 776 3089 2277
Fragments arranged by size:
3089 2277 776
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 2568 3159 3314 4203 5359 1790
Size: 778 591 155 889 1156 2573
Fragments arranged by size:
2573 1156 889 778 591 155
BmgBI CAC'GTC
Cuts at: 1318 2169 2791 1318
Size: 851 622 4669
Fragments arranged by size:
4669 851 622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3873 4831 442
Size: 416 53 83 399 24 2456 958 1753
Fragments arranged by size:
2456 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 2375 3161 4802 1239
Size: 1025 111 786 1641 2579
Fragments arranged by size:
2579 1641 1025 786 111
Bpu10I CC'TnA_GC
Cuts at: 3383 3383
Size: 6142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4267 5132 5430 4267
Size: 865 298 4979
Fragments arranged by size:
4979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4805 882
Size: 617 3306 2219
Fragments arranged by size:
3306 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3933 493
Size: 711 2729 2702
Fragments arranged by size:
2729 2702 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3949 4331 4966 275
Size: 53 83 186 936 2416 382 635 1451
Fragments arranged by size:
2416 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2385 4575 4973 5247 5394 5805 2385
Size: 2190 398 274 147 411 2722
Fragments arranged by size:
2722 2190 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3260 3290 1178
Size: 30 165 30 1857 30 4030
Fragments arranged by size:
4030 1857 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6142
BseYI C'CCAG_C
Cuts at: 2106 2173 2257 2448 2946 3282 5345 5841 2106
Size: 67 84 191 498 336 2063 496 2407
Fragments arranged by size:
2407 2063 498 496 336 191 84 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2612 3371 1163
Size: 573 876 759 3934
Fragments arranged by size:
3934 876 759 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2677 3592 4353 5381 1540
Size: 275 186 676 915 761 1028 2301
Fragments arranged by size:
2301 1028 915 761 676 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2076 2346 2625 2777 4203 4288 5359 2034
Size: 42 270 279 152 1426 85 1071 2817
Fragments arranged by size:
2817 1426 1071 279 270 152 85 42
BsmI GAATG_Cn'
Cuts at: 2461 2498 3653 3746 2461
Size: 37 1155 93 4857
Fragments arranged by size:
4857 1155 93 37
BsmBI CGTCTCn'nnnn_
Cuts at: 2189 2189
Size: 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2076 2346 2568 2625 2777 3159
Size: 399 244 42 270 222 57 152 382
Cuts at: 3159 3314 3461 4203 4288 5359 1391
Size: 155 147 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 270 244 222
155 152 147 85 57 42
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 4031 5761 4031
Size: 1730 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2634 2925 3497 844
Size: 1226 564 291 572 3489
Fragments arranged by size:
3489 1226 572 564 291
BsrBI CCG'CTC
Cuts at: 1394 1823 4029 1394
Size: 429 2206 3507
Fragments arranged by size:
3507 2206 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3412 4637 4811 65
Size: 1183 2164 1225 174 1396
Fragments arranged by size:
2164 1396 1225 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2325 2985 3303 4786 4973 1700
Size: 110 515 660 318 1483 187 2869
Fragments arranged by size:
2869 1483 660 515 318 187 110
BsrGI T'GTAC_A
Cuts at: 96 3267 96
Size: 3171 2971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstXI CCAn_nnnn'nTGG
Cuts at: 3053 3220 3053
Size: 167 5975
BstYI r'GATC_y
Cuts at: 1972 2272 2388 3048 3069 3472 3839 4224 4241
Size: 300 116 660 21 403 367 385 17
Cuts at: 4241 5693 5779 5791 6137 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 660 403 385 367 346 300
116 86 21 17 12
BstZ17I GTA'TAC
Cuts at: 2337 2337
Size: 6142
BtgI C'CryG_G
Cuts at: 513 1065 2780 2901 3315 513
Size: 552 1715 121 414 3340
Fragments arranged by size:
3340 1715 552 414 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 2512 2914 2957 3187 3658 4452 4472 2512
Size: 402 43 230 471 794 20 4182
Fragments arranged by size:
4182 794 471 402 230 43 20
ClaI AT'CG_AT
Cuts at: 1554 2400 1554
Size: 846 5296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DraIII CAC_nnn'GTG
Cuts at: 2772 2887 2772
Size: 115 6027
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2375 3589 4478
Size: 54 1425 50 275 18 545 1214 889
Cuts at: 4478 6107 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1214 889 545 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2187 2480 3083 3219 4072 1353
Size: 662 172 293 603 136 853 3423
Fragments arranged by size:
3423 853 662 603 293 172 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4729 5103 5249 387
Size: 1064 138 3140 374 146 1280
Fragments arranged by size:
3140 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2375 3041 3161 4205 4802 5589
Size: 393 632 111 666 120 1044 597 787
Cuts at: 5589 5857 1239
Size: 268 1524
Fragments arranged by size:
1524 1044 787 666 632 597 393 268
120 111
EcoO109I rG'GnC_Cy
Cuts at: 2476 2893 3310 3311 2476
Size: 417 417 1 5307
Fragments arranged by size:
5307 417 417 1
EcoRI G'AATT_C
Cuts at: 2528 3451 3829 2528
Size: 923 378 4841
Fragments arranged by size:
4841 923 378
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 2814 5289 1678
Size: 1136 2475 2531
Fragments arranged by size:
2531 2475 1136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3040 3072 4783 4815 4857
Size: 32 135 32 1468 32 1711 32 42
Cuts at: 4857 4889 1373
Size: 32 2626
Fragments arranged by size:
2626 1711 1468 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2260 2900 5256 5440 1305
Size: 955 640 2356 184 2007
Fragments arranged by size:
2356 2007 955 640 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4320
Fragments arranged by size:
4320 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 3326 4100 4459 4618 518
Size: 648 1140 1020 774 359 159 2042
Fragments arranged by size:
2042 1140 1020 774 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2353 2452 2640 3558 4235 5383 5628
Size: 504 243 99 188 918 677 1148 245
Cuts at: 5628 1606
Size: 2120
Fragments arranged by size:
2120 1148 918 677 504 245 243 188
99
NaeI GCC'GGC
Cuts at: 2327 2987 2327
Size: 660 5482
NcoI C'CATG_G
Cuts at: 513 1065 2780 513
Size: 552 1715 3875
Fragments arranged by size:
3875 1715 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NgoMIV G'CCGG_C
Cuts at: 2325 2985 2325
Size: 660 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 3500 5045 1132
Size: 97 2271 1545 2229
Fragments arranged by size:
2271 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2433 1348
Size: 1085 5057
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 2887 3331 1108
Size: 1245 534 444 3919
Fragments arranged by size:
3919 1245 534 444
PfoI T'CCnGG_A
Cuts at: 2391 2937 3219 2391
Size: 546 282 5314
Fragments arranged by size:
5314 546 282
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 2476
Size: 6142
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PspOMI G'GGCC_C
Cuts at: 3310 3310
Size: 6142
PstI C_TGCA'G
Cuts at: 830 2334 3165 3494 830
Size: 1504 831 329 3478
Fragments arranged by size:
3478 1504 831 329
PvuI CG_AT'CG
Cuts at: 2001 2677 2001
Size: 676 5466
PvuII CAG'CTG
Cuts at: 2110 2353 2452 2640 2110
Size: 243 99 188 5612
Fragments arranged by size:
5612 243 188 99
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SanDI GG'GwC_CC
Cuts at: 2476 2476
Size: 6142
SapI GCTCTTCn'nnn_
Cuts at: 2015 3083 3219 2015
Size: 1068 136 4938
Fragments arranged by size:
4938 1068 136
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SexAI A'CCwGG_T
Cuts at: 2799 2799
Size: 6142
SfcI C'TryA_G
Cuts at: 826 1045 2330 3161 3490 4625 5306 5497 826
Size: 219 1285 831 329 1135 681 191 1471
Fragments arranged by size:
1471 1285 1135 831 681 329 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4246 5147 5409 820
Size: 197 926 2303 901 262 1553
Fragments arranged by size:
2303 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2215 2780 3237 3581 513
Size: 552 1150 565 457 344 3074
Fragments arranged by size:
3074 1150 565 552 457 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4169 4186 4339 4524 4969 325
Size: 1255 2589 17 153 185 445 1498
Fragments arranged by size:
2589 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2613 3267 4388
Size: 274 80 33 51 1443 636 654 1121
Cuts at: 4388 6100 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1121 654 636 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6102 6134 6102
Size: 32 6110
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 4271 4271
Size: 6142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI