Restriction map for
pFN21AB7725
(Circular) (Six-base) MAPSORT of: pFN21AB7725.seq Check: 5221 from: 1 to: 5974
pFN21AB7725 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 2315 3295 2315
Size: 980 4994
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2449 2617 2701 2953 4037 5421 5689 1632
Size: 817 168 84 252 1084 1384 268 1917
Fragments arranged by size:
1917 1384 1084 817 268 252 168 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 3029 4873 5850 1203
Size: 1826 1844 977 1327
Fragments arranged by size:
1844 1826 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 2955 1166
Size: 1789 4185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 2703 3717 5289 2703
Size: 1014 1572 3388
Fragments arranged by size:
3388 1572 1014
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2304 2751 3283 3509 3661 5770 1493
Size: 811 447 532 226 152 2109 1697
Fragments arranged by size:
2109 1697 811 532 447 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 1870
Size: 73 1356 4545
Fragments arranged by size:
4545 1356 73
AvrII C'CTAG_G
Cuts at: 2249 2249
Size: 5974
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 3295 4750 618
Size: 325 217 627 528 980 1455 1842
Fragments arranged by size:
1842 1455 980 627 528 325 217
BanII G_rGCy'C
Cuts at: 1391 2085 3293 1391
Size: 694 1208 4072
Fragments arranged by size:
4072 1208 694
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2455 928
Size: 971 556 4447
Fragments arranged by size:
4447 971 556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2360 2394 4165 4199 1903
Size: 34 423 34 1771 34 3678
Fragments arranged by size:
3678 1771 423 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BfrBI ATG'CAT
Cuts at: 3259 3259
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4585 136
Size: 107 122 71 631 605 337 2576 1525
Fragments arranged by size:
2576 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2558 2642 5969 2558
Size: 84 3327 2563
Fragments arranged by size:
3327 2563 84
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 4035 5191 1790
Size: 2245 1156 2573
Fragments arranged by size:
2573 2245 1156
BmgBI CAC'GTC
Cuts at: 1318 2194 1318
Size: 876 5098
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2237 3705 4663
Size: 416 53 83 399 24 820 1468 958
Cuts at: 4663 442
Size: 1753
Fragments arranged by size:
1753 1468 958 820 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2324 2450 2916 2324
Size: 126 466 5382
Fragments arranged by size:
5382 466 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2288 2529 2781 3033 4634 1239
Size: 983 66 241 252 252 1601 2579
Fragments arranged by size:
2579 1601 983 252 252 241 66
Bpu10I CC'TnA_GC
Cuts at: 2364 2364
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2767 4099 4964 5262 2767
Size: 1332 865 298 3479
Fragments arranged by size:
3479 1332 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2058 2088 1178
Size: 30 165 30 655 30 5064
Fragments arranged by size:
5064 655 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2989 1186
Size: 976 827 4171
Fragments arranged by size:
4171 976 827
BseYI C'CCAG_C
Cuts at: 2262 5177 5673 2262
Size: 2915 496 2563
Fragments arranged by size:
2915 2563 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3136 1163
Size: 573 1400 4001
Fragments arranged by size:
4001 1400 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 4035 4120 5191 2085
Size: 1950 85 1071 2868
Fragments arranged by size:
2868 1950 1071 85
BsmI GAATG_Cn'
Cuts at: 2615 2699 2919 2951 3261 3485 3578 2615
Size: 84 220 32 310 224 93 5011
Fragments arranged by size:
5011 310 224 220 93 84 32
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 3293 4035 4120 5191 1391
Size: 399 295 1208 742 85 1071 2174
Fragments arranged by size:
2174 1208 1071 742 399 295 85
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2156 2620 4469 4643 65
Size: 1183 908 464 1849 174 1396
Fragments arranged by size:
1849 1396 1183 908 464 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 2184 96
Size: 2088 3886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 2890 4034 5046 2890
Size: 1144 1012 3818
Fragments arranged by size:
3818 1144 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 2380 4778 2380
Size: 2398 3576
BstEII G'GTnAC_C
Cuts at: 1184 2110 1184
Size: 926 5048
BstXI CCAn_nnnn'nTGG
Cuts at: 2957 2957
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2558 2642 3304 3671 4056 4073 5525 5611
Size: 586 84 662 367 385 17 1452 86
Cuts at: 5611 5623 5969 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 662 586 385 367 346 86
84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2130 513
Size: 552 939 126 4357
Fragments arranged by size:
4357 939 552 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2536 2704 3124 3490 4284 4304 2536
Size: 168 420 366 794 20 4206
Fragments arranged by size:
4206 794 420 366 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2602 2637 529
Size: 35 2038 35 3866
Fragments arranged by size:
3866 2038 35 35
DraI TTT'AAA
Cuts at: 2344 2458 3278 2344
Size: 114 820 5040
Fragments arranged by size:
5040 820 114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 3421 4310
Size: 54 1425 50 275 18 297 1294 889
Cuts at: 4310 5939 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1294 889 297 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2289 3904 1353
Size: 936 1615 3423
Fragments arranged by size:
3423 1615 936
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 4561 4935 5081 387
Size: 1064 138 490 2482 374 146 1280
Fragments arranged by size:
2482 1280 1064 490 374 146 138
EcoICRI GAG'CTC
Cuts at: 2083 2083
Size: 5974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2288 2449 2529 2617 2701 2781
Size: 393 590 66 161 80 88 84 80
Cuts at: 2781 2953 3033 4037 4634 5421 5689 1239
Size: 172 80 1004 597 787 268 1524
Fragments arranged by size:
1524 1004 787 597 590 393 268 172
161 88 84 80 80 80 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2971 2971
Size: 5974
EcoRI G'AATT_C
Cuts at: 2304 2751 3283 3661 2304
Size: 447 532 378 4617
Fragments arranged by size:
4617 532 447 378
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2404 2436 2968 3000 4615
Size: 32 135 32 832 32 532 32 1615
Cuts at: 4615 4647 4689 4721 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1615 832 532 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2358 3318 3564 669
Size: 988 281 420 960 246 3079
Fragments arranged by size:
3079 988 960 420 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 2358 3564 2358
Size: 1206 4768
KpnI G_GTAC'C
Cuts at: 2319 3299 2319
Size: 980 4994
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2955 3932 4291 4450 518
Size: 648 1789 977 359 159 2042
Fragments arranged by size:
2042 1789 977 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2266 3390 4067 5215 5460 1606
Size: 660 1124 677 1148 245 2120
Fragments arranged by size:
2120 1148 1124 677 660 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NsiI A_TGCA'T
Cuts at: 3261 3261
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2375 3033 3332 4877 1132
Size: 97 1146 658 299 1545 2229
Fragments arranged by size:
2229 1545 1146 658 299 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 3029 4873 3029
Size: 1844 4130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2207 2239 4043 4075 5556 5588 2207
Size: 32 1804 32 1481 32 2593
Fragments arranged by size:
2593 1804 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PstI C_TGCA'G
Cuts at: 830 2213 2531 3326 830
Size: 1383 318 795 3478
Fragments arranged by size:
3478 1383 795 318
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2266 2266
Size: 5974
SacI G_AGCT'C
Cuts at: 2085 2085
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2209 2527 2548 2914 3322 4457 5138
Size: 219 1164 318 21 366 408 1135 681
Cuts at: 5138 5329 826
Size: 191 1471
Fragments arranged by size:
1471 1164 1135 681 408 366 318 219
191 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2746 4078 4979 5241 820
Size: 197 926 803 1332 901 262 1553
Fragments arranged by size:
1553 1332 926 901 803 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 2249 3413 513
Size: 552 939 245 1164 3074
Fragments arranged by size:
3074 1164 939 552 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2156 4001 4018 4171 4356 4801 325
Size: 1255 576 1845 17 153 185 445 1498
Fragments arranged by size:
1845 1498 1255 576 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 4220 5932
Size: 274 80 33 51 1443 207 2036 1712
Cuts at: 5932 96
Size: 138
Fragments arranged by size:
2036 1712 1443 274 207 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5934 5966 5934
Size: 32 5942
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 2393 3310 2393
Size: 917 5057
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 2630 2714 3218 4103 2630
Size: 84 504 885 4501
Fragments arranged by size:
4501 885 504 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI