Restriction map for
pFN21AB7721
(Circular) (Six-base) MAPSORT of: pFN21AB7721.seq Check: 5624 from: 1 to: 5989
pFN21AB7721 5989 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3122 4052 5436 5704 1632
Size: 1490 930 1384 268 1917
Fragments arranged by size:
1917 1490 1384 930 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 2175 2619 4888 5865 1203
Size: 972 444 2269 977 1327
Fragments arranged by size:
2269 1327 977 972 444
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 2523 1166
Size: 1357 4632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5989
AlwNI CAG_nnn'CTG
Cuts at: 3067 3732 5304 3067
Size: 665 1572 3752
Fragments arranged by size:
3752 1572 665
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 3298 3524 3676 5785 1493
Size: 1805 226 152 2109 1697
Fragments arranged by size:
2109 1805 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2297 2490 3314 1870
Size: 73 354 193 824 4545
Fragments arranged by size:
4545 824 354 193 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3047 3080 3047
Size: 33 5956
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3229 3310 4765 618
Size: 325 217 627 1442 81 1455 1842
Fragments arranged by size:
1842 1455 1442 627 325 217 81
BanII G_rGCy'C
Cuts at: 1391 2304 3308 1391
Size: 913 1004 4072
Fragments arranged by size:
4072 1004 913
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2137 928
Size: 971 238 4780
Fragments arranged by size:
4780 971 238
BbvCI CC'TCA_GC
Cuts at: 2816 2816
Size: 5989
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2787 2821 2981 3015 3022 3056 4180
Size: 34 850 34 160 34 7 34 1124
Cuts at: 4180 4214 1903
Size: 34 3678
Fragments arranged by size:
3678 1124 850 160 34 34 34 34
34 7
BciVI GTATCCnnnnn_n'
Cuts at: 3881 5091 3881
Size: 1210 4779
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4600 136
Size: 107 122 71 631 605 2928 1525
Fragments arranged by size:
2928 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2367 5984 2367
Size: 3617 2372
BlpI GC'TnA_GC
Cuts at: 3406 3406
Size: 5989
Bme1580I G_kGCm'C
Cuts at: 1790 2670 4050 5206 1790
Size: 880 1380 1156 2573
Fragments arranged by size:
2573 1380 1156 880
BmgBI CAC'GTC
Cuts at: 1318 2041 3142 1318
Size: 723 1101 4165
Fragments arranged by size:
4165 1101 723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2282 2398 3720
Size: 416 53 83 399 24 865 116 1322
Cuts at: 3720 4678 442
Size: 958 1753
Fragments arranged by size:
1753 1322 958 865 416 399 116 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 2750 4649 1239
Size: 1250 261 1899 2579
Fragments arranged by size:
2579 1899 1250 261
Bpu10I CC'TnA_GC
Cuts at: 2816 2816
Size: 5989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 4114 4979 5277 2462
Size: 1652 865 298 3174
Fragments arranged by size:
3174 1652 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2129 3246 4652 882
Size: 617 630 1117 1406 2219
Fragments arranged by size:
2219 1406 1117 630 617
BsaAI yAC'GTr
Cuts at: 493 1204 2754 3780 493
Size: 711 1550 1026 2702
Fragments arranged by size:
2702 1550 1026 711
BsaBI GATnn'nnATC
Cuts at: 2462 5983 2462
Size: 3521 2468
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2147 2177 1178
Size: 30 165 30 744 30 4990
Fragments arranged by size:
4990 744 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2981 3206 3221 1186
Size: 1795 225 15 3954
Fragments arranged by size:
3954 1795 225 15
BseYI C'CCAG_C
Cuts at: 2352 2881 5192 5688 2352
Size: 529 2311 496 2653
Fragments arranged by size:
2653 2311 529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 1163
Size: 573 651 4765
Fragments arranged by size:
4765 651 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 2766 4050 4135 5206 2499
Size: 267 1284 85 1071 3282
Fragments arranged by size:
3282 1284 1071 267 85
BsmI GAATG_Cn'
Cuts at: 2843 3500 3593 2843
Size: 657 93 5239
Fragments arranged by size:
5239 657 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2499 2670 2766 3308 4050 4135
Size: 399 514 195 171 96 542 742 85
Cuts at: 4135 5206 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 542 514 399 195 171
96 85
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 2565 3136 3878 5608 2565
Size: 571 742 1730 2946
Fragments arranged by size:
2946 1730 742 571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3344 844
Size: 2500 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3876 1394
Size: 429 2053 3507
Fragments arranged by size:
3507 2053 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4484 4658 65
Size: 1183 3236 174 1396
Fragments arranged by size:
3236 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2716 4633 4820 1700
Size: 110 906 1917 187 2869
Fragments arranged by size:
2869 1917 906 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 2876 4049 5061 2876
Size: 1173 1012 3804
Fragments arranged by size:
3804 1173 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5989
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2017
Size: 5989
BstYI r'GATC_y
Cuts at: 1972 2367 3075 3319 3686 4071 4088 5540 5626
Size: 395 708 244 367 385 17 1452 86
Cuts at: 5626 5638 5984 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 708 395 385 367 346 244
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3204 3204
Size: 5989
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2521 513
Size: 552 939 12 505 3981
Fragments arranged by size:
3981 939 552 505 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2069 2292 2922 3291 487
Size: 683 56 843 223 630 369 3185
Fragments arranged by size:
3185 843 683 630 369 223 56
BtsI GCAGTG_nn'
Cuts at: 3505 4299 4319 3505
Size: 794 20 5175
Fragments arranged by size:
5175 794 20
ClaI AT'CG_AT
Cuts at: 1554 2797 1554
Size: 1243 4746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2740 2775 529
Size: 35 2176 35 3743
Fragments arranged by size:
3743 2176 35 35
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DraIII CAC_nnn'GTG
Cuts at: 3125 3125
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2644 3436
Size: 54 1425 50 275 18 177 637 792
Cuts at: 3436 4325 5954 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 792 637 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2946 3919 1353
Size: 1593 973 3423
Fragments arranged by size:
3423 1593 973
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3068 4576 4950 5096 387
Size: 1064 138 1479 1508 374 146 1280
Fragments arranged by size:
1508 1479 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2489 2750 3122 4052 4649 5436 5704
Size: 393 857 261 372 930 597 787 268
Cuts at: 5704 1239
Size: 1524
Fragments arranged by size:
1524 930 857 787 597 393 372 268
261
EcoNI CCTnn'n_nnAGG
Cuts at: 2610 3166 2610
Size: 556 5433
EcoO109I rG'GnC_Cy
Cuts at: 3200 3200
Size: 5989
EcoRI G'AATT_C
Cuts at: 3298 3676 3298
Size: 378 5611
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 5136 1678
Size: 3458 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4630 4662 4704 4736 1373
Size: 32 135 32 3058 32 42 32 2626
Fragments arranged by size:
3058 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2446 3333 3579 669
Size: 988 281 508 887 246 3079
Fragments arranged by size:
3079 988 887 508 281 246
HindIII A'AGCT_T
Cuts at: 748 2541 748
Size: 1793 4196
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5103 5287 1305
Size: 3798 184 2007
Fragments arranged by size:
3798 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3990
Fragments arranged by size:
3990 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2234 2523 2570 3024 3261 3947 4306
Size: 648 1068 289 47 454 237 686 359
Cuts at: 4306 4465 518
Size: 159 2042
Fragments arranged by size:
2042 1068 686 648 454 359 289 237
159 47
MspA1I CmG'CkG
Cuts at: 1606 2356 2919 3405 4082 5230 5475 1606
Size: 750 563 486 677 1148 245 2120
Fragments arranged by size:
2120 1148 750 677 563 486 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2521 513
Size: 552 939 517 3981
Fragments arranged by size:
3981 939 552 517
NdeI CA'TA_TG
Cuts at: 387 2929 387
Size: 2542 3447
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 2179 2623 3347 4892 1132
Size: 97 950 444 724 1545 2229
Fragments arranged by size:
2229 1545 950 724 444 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5989
PciI A'CATG_T
Cuts at: 2175 2619 4888 2175
Size: 444 2269 3276
Fragments arranged by size:
3276 2269 444
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2089 2107 2388 2734 3067 1108
Size: 981 18 281 346 333 4030
Fragments arranged by size:
4030 981 346 333 281 18
PfoI T'CCnGG_A
Cuts at: 2466 2466
Size: 5989
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PmlI CAC'GTG
Cuts at: 2754 2754
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3200 3200
Size: 5989
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5989
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PstI C_TGCA'G
Cuts at: 830 2217 3151 3341 830
Size: 1387 934 190 3478
Fragments arranged by size:
3478 1387 934 190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 2356 2356
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 2996 4237 5949 2996
Size: 1241 1712 3036
Fragments arranged by size:
3036 1712 1241
SexAI A'CCwGG_T
Cuts at: 2727 2727
Size: 5989
SfcI C'TryA_G
Cuts at: 826 1045 2213 2977 3147 3214 3337 4472 5153
Size: 219 1168 764 170 67 123 1135 681
Cuts at: 5153 5344 826
Size: 191 1471
Fragments arranged by size:
1471 1168 1135 764 681 219 191 170
123 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 2299 3316 1872
Size: 427 1017 4545
Fragments arranged by size:
4545 1017 427
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2477 4093 4994 5256 820
Size: 197 926 534 1616 901 262 1553
Fragments arranged by size:
1616 1553 926 901 534 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 3913 5
Size: 47 3861 2081
Fragments arranged by size:
3861 2081 47
StuI AGG'CCT
Cuts at: 1517 2162 2426 1517
Size: 645 264 5080
Fragments arranged by size:
5080 645 264
StyI C'CwwG_G
Cuts at: 513 1065 2004 2521 3428 513
Size: 552 939 517 907 3074
Fragments arranged by size:
3074 939 907 552 517
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2873 3247 4016 4033 4186 4371 4816
Size: 1255 1293 374 769 17 153 185 445
Cuts at: 4816 325
Size: 1498
Fragments arranged by size:
1498 1293 1255 769 445 374 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2994 4235 5947
Size: 274 80 33 51 1443 1017 1241 1712
Cuts at: 5947 96
Size: 138
Fragments arranged by size:
1712 1443 1241 1017 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3004 3036 3111 3143 5949 5981 3004
Size: 32 75 32 2806 32 3012
Fragments arranged by size:
3012 2806 75 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2561 2572 1316
Size: 1245 11 4733
Fragments arranged by size:
4733 1245 11
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2060 2060
Size: 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 2297 3314 1870
Size: 427 1017 4545
Fragments arranged by size:
4545 1017 427
XmnI GAAnn'nnTTC
Cuts at: 4118 4118
Size: 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI