Restriction map for
pFN21AB7714
(Circular) (Six-base) MAPSORT of: pFN21AB7714.seq Check: 6915 from: 1 to: 6124
pFN21AB7714 6124 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2062 3047 2062
Size: 985 5139
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2896 2969 3131 3260 3314 4187 5571 5839
Size: 1264 73 162 129 54 873 1384 268
Cuts at: 5839 1632
Size: 1917
Fragments arranged by size:
1917 1384 1264 873 268 162 129 73
54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 2291 2856 2868 5023 6000 1203
Size: 1088 565 12 2155 977 1327
Fragments arranged by size:
2155 1327 1088 977 565 12
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 2182 4853 2182
Size: 2671 3453
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2091 1184
Size: 907 5217
AlwNI CAG_nnn'CTG
Cuts at: 2186 2533 2548 2664 2787 3352 3867 5439 2186
Size: 347 15 116 123 565 515 1572 2871
Fragments arranged by size:
2871 1572 565 515 347 123 116 15
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 2632 3095 3433 3659 3811 5920 1493
Size: 1139 463 338 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 463 338 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 3449 1870
Size: 73 1506 4545
Fragments arranged by size:
4545 1506 73
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2349 2366 3445 4900 618
Size: 325 217 627 562 17 1079 1455 1842
Fragments arranged by size:
1842 1455 1079 627 562 325 217 17
BanII G_rGCy'C
Cuts at: 1391 3087 3321 3443 1391
Size: 1696 234 122 4072
Fragments arranged by size:
4072 1696 234 122
BbeI G_GCGC'C
Cuts at: 2370 2370
Size: 6124
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2393 3054 928
Size: 971 494 661 3998
Fragments arranged by size:
3998 971 661 494
BbvCI CC'TCA_GC
Cuts at: 2345 2345
Size: 6124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4315 4349 1903
Size: 34 2378 34 3678
Fragments arranged by size:
3678 2378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4016 5226 4016
Size: 1210 4914
BclI T'GATC_A
Cuts at: 1548 2454 2493 2571 1548
Size: 906 39 78 5101
Fragments arranged by size:
5101 906 78 39
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4735 136
Size: 107 122 71 631 605 3063 1525
Fragments arranged by size:
3063 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2734 2826 2937 6119 2734
Size: 92 111 3182 2739
Fragments arranged by size:
3182 2739 111 92
BlpI GC'TnA_GC
Cuts at: 2654 3336 3541 2654
Size: 682 205 5237
Fragments arranged by size:
5237 682 205
Bme1580I G_kGCm'C
Cuts at: 1790 2354 4185 5341 1790
Size: 564 1831 1156 2573
Fragments arranged by size:
2573 1831 1156 564
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2196 2543 3855
Size: 416 53 83 399 24 779 347 1312
Cuts at: 3855 4813 442
Size: 958 1753
Fragments arranged by size:
1753 1312 958 779 416 399 347 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2450 3101 4784 1239
Size: 905 306 651 1683 2579
Fragments arranged by size:
2579 1683 905 651 306
Bpu10I CC'TnA_GC
Cuts at: 2345 2345
Size: 6124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4249 5114 5412 4249
Size: 865 298 4961
Fragments arranged by size:
4961 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2125 2758 4787 882
Size: 617 626 633 2029 2219
Fragments arranged by size:
2219 2029 633 626 617
BsaAI yAC'GTr
Cuts at: 493 1204 3915 493
Size: 711 2711 2702
Fragments arranged by size:
2711 2702 711
BsaBI GATnn'nnATC
Cuts at: 2570 6118 2570
Size: 3548 2576
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3931 4313 4948
Size: 53 83 186 936 834 1564 382 635
Cuts at: 4948 275
Size: 1451
Fragments arranged by size:
1564 1451 936 834 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2358 2727 4557 4955 5229 5376 5787 2358
Size: 369 1830 398 274 147 411 2695
Fragments arranged by size:
2695 1830 411 398 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2924 2954 1178
Size: 30 165 30 1521 30 4348
Fragments arranged by size:
4348 1521 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 2570 3146 3149 1186
Size: 1306 78 576 3 4161
Fragments arranged by size:
4161 1306 576 78 3
BseYI C'CCAG_C
Cuts at: 2803 3267 3345 5327 5823 2803
Size: 464 78 1982 496 3104
Fragments arranged by size:
3104 1982 496 464 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 2561 1163
Size: 573 315 510 4726
Fragments arranged by size:
4726 573 510 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 4335 5363 1540
Size: 275 186 1573 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2053 2744 3321 4185 4270 5341 2053
Size: 691 577 864 85 1071 2836
Fragments arranged by size:
2836 1071 864 691 577 85
BsmI GAATG_Cn'
Cuts at: 3260 3635 3728 3260
Size: 375 93 5656
Fragments arranged by size:
5656 375 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2354 2744 3087 3321 3443 4185
Size: 399 263 301 390 343 234 122 742
Cuts at: 4185 4270 5341 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 343 301 263
234 122 85
BspEI T'CCGG_A
Cuts at: 2358 2727 5787 2358
Size: 369 3060 2695
Fragments arranged by size:
3060 2695 369
BspHI T'CATG_A
Cuts at: 2286 4013 5743 2286
Size: 1727 1730 2667
Fragments arranged by size:
2667 1730 1727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2062 3047 3479 844
Size: 1218 985 432 3489
Fragments arranged by size:
3489 1218 985 432
BsrBI CCG'CTC
Cuts at: 1394 1823 2436 4011 1394
Size: 429 613 1575 3507
Fragments arranged by size:
3507 1575 613 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4619 4793 65
Size: 1183 3371 174 1396
Fragments arranged by size:
3371 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3027 4768 4955 1700
Size: 110 1217 1741 187 2869
Fragments arranged by size:
2869 1741 1217 187 110
BsrGI T'GTAC_A
Cuts at: 96 2164 96
Size: 2068 4056
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2664 794
Size: 1870 4254
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6124
BstYI r'GATC_y
Cuts at: 1972 2734 2826 2937 3454 3821 4206 4223 5675
Size: 762 92 111 517 367 385 17 1452
Cuts at: 5675 5761 5773 6119 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 762 517 385 367 346 111
92 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2472 513
Size: 552 1407 4165
Fragments arranged by size:
4165 1407 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 2665 2788 3051 3640 4434 4454 2665
Size: 123 263 589 794 20 4335
Fragments arranged by size:
4335 794 589 263 123 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 3428 3428
Size: 6124
DraIII CAC_nnn'GTG
Cuts at: 3202 3202
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3025 3571 4460
Size: 54 1425 50 275 18 1195 546 889
Cuts at: 4460 6089 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1195 889 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3170 4054 1353
Size: 1817 884 3423
Fragments arranged by size:
3423 1817 884
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 5085 5231 387
Size: 1064 138 3122 374 146 1280
Fragments arranged by size:
3122 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3319 3319
Size: 6124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2450 2896 2969 3101 3131 3260
Size: 393 512 306 446 73 132 30 129
Cuts at: 3260 3314 4187 4784 5571 5839 1239
Size: 54 873 597 787 268 1524
Fragments arranged by size:
1524 873 787 597 512 446 393 306
268 132 129 73 54 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2911 2948 3395 2911
Size: 37 447 5640
Fragments arranged by size:
5640 447 37
EcoO109I rG'GnC_Cy
Cuts at: 2040 2231 2689 2040
Size: 191 458 5475
Fragments arranged by size:
5475 458 191
EcoRI G'AATT_C
Cuts at: 3433 3811 3433
Size: 378 5746
FspI TGC'GCA
Cuts at: 2420 3359 4630 2420
Size: 939 1271 3914
Fragments arranged by size:
3914 1271 939
HaeII r_GCGC'y
Cuts at: 1678 2370 5271 1678
Size: 692 2901 2531
Fragments arranged by size:
2901 2531 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 2509 2541 2575
Size: 32 135 32 596 32 309 32 34
Cuts at: 2575 2607 2726 2758 2924 2956 4765 4797 4839
Size: 32 119 32 166 32 1809 32 42
Cuts at: 4839 4871 1373
Size: 32 2626
Fragments arranged by size:
2626 1809 596 309 166 135 119 42
34 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 2862 2975 3468 3714 669
Size: 988 281 271 653 113 493 246 3079
Fragments arranged by size:
3079 988 653 493 281 271 246 113
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KasI G'GCGC_C
Cuts at: 2366 2366
Size: 6124
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2206 2487 5238 5422 1305
Size: 901 281 2751 184 2007
Fragments arranged by size:
2751 2007 901 281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4302
Fragments arranged by size:
4302 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2280 3305 4082 4441 4600 518
Size: 648 1114 1025 777 359 159 2042
Fragments arranged by size:
2042 1114 1025 777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2563 2764 3124 3214 3349 3540 4217 5365
Size: 957 201 360 90 135 191 677 1148
Cuts at: 5365 5610 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 957 677 360 245 201 191
135 90
NaeI GCC'GGC
Cuts at: 3029 3029
Size: 6124
NarI GG'CG_CC
Cuts at: 2367 2367
Size: 6124
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5572
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NgoMIV G'CCGG_C
Cuts at: 3027 3027
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 2295 2860 2872 3109 3482 5027 1132
Size: 97 1066 565 12 237 373 1545 2229
Fragments arranged by size:
2229 1545 1066 565 373 237 97 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6124
PciI A'CATG_T
Cuts at: 2291 2856 2868 5023 2291
Size: 565 12 2155 3392
Fragments arranged by size:
3392 2155 565 12
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6124
PfoI T'CCnGG_A
Cuts at: 2191 3363 2191
Size: 1172 4952
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 5706 5738 4193
Size: 32 1481 32 4579
Fragments arranged by size:
4579 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2040 2231 2040
Size: 191 5933
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PstI C_TGCA'G
Cuts at: 830 2688 2763 3058 3123 3195 3219 3476 830
Size: 1858 75 295 65 72 24 257 3478
Fragments arranged by size:
3478 1858 295 257 75 72 65 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
PvuII CAG'CTG
Cuts at: 2764 3124 3214 2764
Size: 360 90 5674
Fragments arranged by size:
5674 360 90
SacI G_AGCT'C
Cuts at: 3321 3321
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SanDI GG'GwC_CC
Cuts at: 2040 2040
Size: 6124
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SfcI C'TryA_G
Cuts at: 826 1045 2684 2759 3054 3119 3191 3215 3472
Size: 219 1639 75 295 65 72 24 257
Cuts at: 3472 4607 5288 5479 826
Size: 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 681 295 257 219 191
75 72 65 24
SfoI GGC'GCC
Cuts at: 2368 2368
Size: 6124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4228 5129 5391 820
Size: 197 926 2285 901 262 1553
Fragments arranged by size:
2285 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3482 1132
Size: 2350 3774
SspI AAT'ATT
Cuts at: 5 52 4048 5
Size: 47 3996 2081
Fragments arranged by size:
3996 2081 47
StuI AGG'CCT
Cuts at: 1517 2408 1517
Size: 891 5233
StyI C'CwwG_G
Cuts at: 513 1065 3563 513
Size: 552 2498 3074
Fragments arranged by size:
3074 2498 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3359 4151 4168 4321 4506 4951 325
Size: 1255 1779 792 17 153 185 445 1498
Fragments arranged by size:
1779 1498 1255 792 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2164 4370 6082
Size: 274 80 33 51 1443 187 2206 1712
Cuts at: 6082 96
Size: 138
Fragments arranged by size:
2206 1712 1443 274 187 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2494 2526 6084 6116 2494
Size: 32 3558 32 2502
Fragments arranged by size:
3558 2502 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 4253 4253
Size: 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SapI SexAI SfiI SrfI SwaI XcmI