Restriction map for
pFN21AB7713
(Circular) (Six-base) MAPSORT of: pFN21AB7713.seq Check: 6358 from: 1 to: 5974
pFN21AB7713 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4037 5421 5689 1632
Size: 2405 1384 268 1917
Fragments arranged by size:
2405 1917 1384 268
AfeI AGC'GCT
Cuts at: 2831 2831
Size: 5974
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 2776 4703 2776
Size: 1927 4047
AleI CACnn'nnGTG
Cuts at: 1166 2277 1166
Size: 1111 4863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3164 1184
Size: 1980 3994
AlwNI CAG_nnn'CTG
Cuts at: 2555 2657 2959 3020 3717 5289 2555
Size: 102 302 61 697 1572 3240
Fragments arranged by size:
3240 1572 697 302 102 61
ApaI G_GGCC'C
Cuts at: 2528 2544 2528
Size: 16 5958
ApaLI G'TGCA_C
Cuts at: 3023 4031 5187 3023
Size: 1008 1156 3810
Fragments arranged by size:
3810 1156 1008
ApoI r'AATT_y
Cuts at: 1493 3283 3509 3661 5770 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2521 3257 3299 1870
Size: 73 578 736 42 4545
Fragments arranged by size:
4545 736 578 73 42
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2664 3226 3295 4750 618
Size: 325 217 627 877 562 69 1455 1842
Fragments arranged by size:
1842 1455 877 627 562 325 217 69
BanII G_rGCy'C
Cuts at: 1391 2528 2544 3293 1391
Size: 1137 16 749 4072
Fragments arranged by size:
4072 1137 749 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2576 928
Size: 971 677 4326
Fragments arranged by size:
4326 971 677
BbvCI CC'TCA_GC
Cuts at: 2865 2865
Size: 5974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2148 2399 3101
Size: 107 122 71 631 605 476 251 702
Cuts at: 3101 3105 4585 136
Size: 4 1480 1525
Fragments arranged by size:
1525 1480 702 631 605 476 251 122
107 71 4
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 2216 3123 3391 2216
Size: 907 268 4799
Fragments arranged by size:
4799 907 268
Bme1580I G_kGCm'C
Cuts at: 1790 2528 2544 2667 2896 3027 3231 4035 5191
Size: 738 16 123 229 131 204 804 1156
Cuts at: 5191 1790
Size: 2573
Fragments arranged by size:
2573 1156 804 738 229 204 131 123
16
BmgBI CAC'GTC
Cuts at: 1318 2257 1318
Size: 939 5035
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2576 2927 3705
Size: 416 53 83 399 24 1159 351 778
Cuts at: 3705 4663 442
Size: 958 1753
Fragments arranged by size:
1753 1159 958 778 416 399 351 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2577 2987 3104 3236 4634 1239
Size: 1338 410 117 132 1398 2579
Fragments arranged by size:
2579 1398 1338 410 132 117
Bpu10I CC'TnA_GC
Cuts at: 2865 2865
Size: 5974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 4099 4964 5262 2423
Size: 1676 865 298 3135
Fragments arranged by size:
3135 1676 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2087 4637 882
Size: 617 588 2550 2219
Fragments arranged by size:
2550 2219 617 588
BsaAI yAC'GTr
Cuts at: 493 1204 3765 493
Size: 711 2561 2702
Fragments arranged by size:
2702 2561 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2086 2116 2246 2276 3150
Size: 30 165 30 683 30 130 30 874
Cuts at: 3150 3180 1178
Size: 30 3972
Fragments arranged by size:
3972 874 683 165 130 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2990 3155 1186
Size: 991 813 165 4005
Fragments arranged by size:
4005 991 813 165
BseYI C'CCAG_C
Cuts at: 2180 2537 2671 2727 2815 2826 5177 5673 2180
Size: 357 134 56 88 11 2351 496 2481
Fragments arranged by size:
2481 2351 496 357 134 88 56 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3053 1163
Size: 573 1317 4084
Fragments arranged by size:
4084 1317 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2498 3100 3424 4185 5213 1540
Size: 275 186 497 602 324 761 1028 2301
Fragments arranged by size:
2301 1028 761 602 497 324 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 3027 4035 4120 5191 2217
Size: 810 1008 85 1071 3000
Fragments arranged by size:
3000 1071 1008 810 85
BsmI GAATG_Cn'
Cuts at: 2593 3485 3578 2593
Size: 892 93 4989
Fragments arranged by size:
4989 892 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2377 2377
Size: 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2528 2544 2667 2896 3027 3231
Size: 399 427 311 16 123 229 131 204
Cuts at: 3231 3293 4035 4120 5191 1391
Size: 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 399 311 229 204
131 123 85 62 16
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3329 844
Size: 2485 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3127 3163 3861 1394
Size: 429 1304 36 698 3507
Fragments arranged by size:
3507 1304 698 429 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 3095 4618 4805 1700
Size: 110 243 1042 1523 187 2869
Fragments arranged by size:
2869 1523 1042 243 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 3071 4034 5046 3071
Size: 963 1012 3999
Fragments arranged by size:
3999 1012 963
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2394 2941 2959 794
Size: 1600 547 18 3809
Fragments arranged by size:
3809 1600 547 18
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 2716 1184
Size: 1532 4442
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 3203 2017
Size: 1186 4788
BstYI r'GATC_y
Cuts at: 1972 3304 3671 4056 4073 5525 5611 5623 5969
Size: 1332 367 385 17 1452 86 12 346
Cuts at: 5969 1972
Size: 1977
Fragments arranged by size:
1977 1452 1332 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2676 513
Size: 552 1611 3811
Fragments arranged by size:
3811 1611 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2739 487
Size: 683 56 1513 3722
Fragments arranged by size:
3722 1513 683 56
BtsI GCAGTG_nn'
Cuts at: 2510 2834 2840 3490 4284 4304 2510
Size: 324 6 650 794 20 4180
Fragments arranged by size:
4180 794 650 324 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DraIII CAC_nnn'GTG
Cuts at: 2418 2418
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2495 3097
Size: 54 1425 50 275 18 225 440 602
Cuts at: 3097 3421 4310 5939 8
Size: 324 889 1629 43
Fragments arranged by size:
1629 1425 889 602 440 324 275 225
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2495 3097 3421 1537
Size: 275 683 602 324 4090
Fragments arranged by size:
4090 683 602 324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2880 3904 1353
Size: 801 726 1024 3423
Fragments arranged by size:
3423 1024 801 726
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3158 4561 4935 5081 387
Size: 1064 138 1569 1403 374 146 1280
Fragments arranged by size:
1569 1403 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2577 2987 3104 3236 4037 4634 5421
Size: 393 945 410 117 132 801 597 787
Cuts at: 5421 5689 1239
Size: 268 1524
Fragments arranged by size:
1524 945 801 787 597 410 393 268
132 117
EcoO109I rG'GnC_Cy
Cuts at: 2525 2541 2561 2654 2956 3076 2525
Size: 16 20 93 302 120 5423
Fragments arranged by size:
5423 302 120 93 20 16
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3262 3262
Size: 5974
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 2236 2833 5121 1678
Size: 558 597 2288 2531
Fragments arranged by size:
2531 2288 597 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 2246 2278 2762
Size: 32 135 32 514 32 128 32 484
Cuts at: 2762 2794 4615 4647 4689 4721 1373
Size: 32 1821 32 42 32 2626
Fragments arranged by size:
2626 1821 514 484 135 128 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2382 5088 5272 1305
Size: 1077 2706 184 2007
Fragments arranged by size:
2706 2007 1077 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2277 3201 3932 4291 4450 518
Size: 648 1111 924 731 359 159 2042
Fragments arranged by size:
2042 1111 924 731 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2346 2727 2903 3055 3179 3390 4067 5215
Size: 740 381 176 152 124 211 677 1148
Cuts at: 5215 5460 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 740 677 381 245 211 176
152 124
NaeI GCC'GGC
Cuts at: 2055 3097 2055
Size: 1042 4932
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5422
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NgoMIV G'CCGG_C
Cuts at: 2053 3095 2053
Size: 1042 4932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2437 3332 4877 1132
Size: 97 1208 895 1545 2229
Fragments arranged by size:
2229 1545 1208 895 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2020 2727 1108
Size: 912 707 4355
Fragments arranged by size:
4355 912 707
PfoI T'CCnGG_A
Cuts at: 2877 2877
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 2524 2540 2524
Size: 16 5958
PspXI vC'TCGA_Gb
Cuts at: 3257 3257
Size: 5974
PstI C_TGCA'G
Cuts at: 830 2790 2847 2955 3326 830
Size: 1960 57 108 371 3478
Fragments arranged by size:
3478 1960 371 108 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2346 2727 2903 3055 2346
Size: 381 176 152 5265
Fragments arranged by size:
5265 381 176 152
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 2784 4222 5934 2784
Size: 1438 1712 2824
Fragments arranged by size:
2824 1712 1438
SfcI C'TryA_G
Cuts at: 826 1045 2786 2843 2951 3322 4457 5138 5329
Size: 219 1741 57 108 371 1135 681 191
Cuts at: 5329 826
Size: 1471
Fragments arranged by size:
1741 1471 1135 681 371 219 191 108
57
SfiI GGCCn_nnn'nGGCC
Cuts at: 2399 3105 2399
Size: 706 5268
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 2523 3301 1872
Size: 651 778 4545
Fragments arranged by size:
4545 778 651
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 3257 4078 4979 5241 820
Size: 197 926 495 819 821 901 262 1553
Fragments arranged by size:
1553 926 901 821 819 495 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 2938 152
Size: 2786 3188
SphI G_CATG'C
Cuts at: 1132 2437 3332 1132
Size: 1305 895 3774
Fragments arranged by size:
3774 1305 895
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 2297 2393 2402 2996 1517
Size: 780 96 9 594 4495
Fragments arranged by size:
4495 780 594 96 9
StyI C'CwwG_G
Cuts at: 513 1065 2997 3413 513
Size: 552 1932 416 3074
Fragments arranged by size:
3074 1932 552 416
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 2718 2807 4001 4018 4171 4356
Size: 1255 531 607 89 1194 17 153 185
Cuts at: 4356 4801 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1194 607 531 445 185 153
89 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2340 2484 2782
Size: 274 80 33 51 1443 363 144 298
Cuts at: 2782 4220 5932 96
Size: 1438 1712 138
Fragments arranged by size:
1712 1443 1438 363 298 274 144 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2502 2534 5934 5966 2502
Size: 32 3400 32 2510
Fragments arranged by size:
3400 2510 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2462 2873 1316
Size: 1146 411 4417
Fragments arranged by size:
4417 1146 411
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2017
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 3257 1943
Size: 1314 4660
XmaI C'CCGG_G
Cuts at: 1870 2521 3299 1870
Size: 651 778 4545
Fragments arranged by size:
4545 778 651
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PsrI
RsrII SacI SacII SanDI SapI SexAI SfoI SrfI
SwaI