Restriction map for
pFN21AB7707
(Circular) (Six-base) MAPSORT of: pFN21AB7707.seq Check: 4308 from: 1 to: 5962
pFN21AB7707 5962 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2387 2387
Size: 5962
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2119 4025 5409 5677 1632
Size: 487 1906 1384 268 1917
Fragments arranged by size:
1917 1906 1384 487 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 2875 4691 2875
Size: 1816 4146
AleI CACnn'nnGTG
Cuts at: 1166 2895 2986 1166
Size: 1729 91 4142
Fragments arranged by size:
4142 1729 91
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2480 1184
Size: 1296 4666
AlwNI CAG_nnn'CTG
Cuts at: 2713 2809 2923 3705 5277 2713
Size: 96 114 782 1572 3398
Fragments arranged by size:
3398 1572 782 114 96
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2967 3287 1870
Size: 73 1024 320 4545
Fragments arranged by size:
4545 1024 320 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2268 2301 2268
Size: 33 5929
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3283 4738 618
Size: 325 217 627 1496 1455 1842
Fragments arranged by size:
1842 1496 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3220 3281 1391
Size: 1829 61 4072
Fragments arranged by size:
4072 1829 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2531 2595 928
Size: 971 162 470 64 4295
Fragments arranged by size:
4295 971 470 162 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4153 4187 1903
Size: 34 50 34 2132 34 3678
Fragments arranged by size:
3678 2132 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2269 3854 5064 2269
Size: 1585 1210 3167
Fragments arranged by size:
3167 1585 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 2835 3379 2835
Size: 544 5418
Bme1580I G_kGCm'C
Cuts at: 1790 4023 5179 1790
Size: 2233 1156 2573
Fragments arranged by size:
2573 2233 1156
BmgBI CAC'GTC
Cuts at: 1318 2646 1318
Size: 1328 4634
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2891 3162 4622 1239
Size: 1652 271 1460 2579
Fragments arranged by size:
2579 1652 1460 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4087 4952 5250 4087
Size: 865 298 4799
Fragments arranged by size:
4799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2225 2229 2957 4625 882
Size: 617 726 4 728 1668 2219
Fragments arranged by size:
2219 1668 728 726 617 4
BsaAI yAC'GTr
Cuts at: 493 1204 2584 3753 493
Size: 711 1380 1169 2702
Fragments arranged by size:
2702 1380 1169 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2789 3769 4151 4786
Size: 53 83 186 936 1256 980 382 635
Cuts at: 4786 275
Size: 1451
Fragments arranged by size:
1451 1256 980 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2021 2051 2205 2235 1178
Size: 30 165 30 618 30 154 30 4905
Fragments arranged by size:
4905 618 165 154 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5962
BseYI C'CCAG_C
Cuts at: 5165 5661 5165
Size: 496 5466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2404 1163
Size: 573 668 4721
Fragments arranged by size:
4721 668 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3056 3412 4173 5201 1540
Size: 275 186 1055 356 761 1028 2301
Fragments arranged by size:
2301 1055 1028 761 356 275 186
BsiHKAI G_wGCw'C
Cuts at: 2090 4023 4108 5179 2090
Size: 1933 85 1071 2873
Fragments arranged by size:
2873 1933 1071 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
BsmBI CGTCTCn'nnnn_
Cuts at: 2636 2636
Size: 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 3220 3281 4023 4108 5179 1391
Size: 399 300 1130 61 742 85 1071 2174
Fragments arranged by size:
2174 1130 1071 742 399 300 85 61
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2387 2784 3317 844
Size: 1543 397 533 3489
Fragments arranged by size:
3489 1543 533 397
BsrBI CCG'CTC
Cuts at: 1394 1823 2327 2931 3849 1394
Size: 429 504 604 918 3507
Fragments arranged by size:
3507 918 604 504 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3055 4606 4793 1700
Size: 110 1245 1551 187 2869
Fragments arranged by size:
2869 1551 1245 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 3241 4022 5034 3241
Size: 781 1012 4169
Fragments arranged by size:
4169 1012 781
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2671 2713 2809 794
Size: 1877 42 96 3947
Fragments arranged by size:
3947 1877 96 42
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 2107 2653 2897 1184
Size: 923 546 244 4249
Fragments arranged by size:
4249 923 546 244
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2584 2988 2047
Size: 537 404 5021
Fragments arranged by size:
5021 537 404
BstYI r'GATC_y
Cuts at: 1972 3108 3292 3659 4044 4061 5513 5599 5611
Size: 1136 184 367 385 17 1452 86 12
Cuts at: 5611 5957 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1136 385 367 346 184 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3031 3192 3031
Size: 161 5801
BtgI C'CryG_G
Cuts at: 513 1065 2040 2280 513
Size: 552 975 240 4195
Fragments arranged by size:
4195 975 552 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2682 3478 4272 4292 2682
Size: 796 794 20 4352
Fragments arranged by size:
4352 796 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 3053 3057
Size: 54 1425 50 275 18 498 725 4
Cuts at: 3057 3409 4298 5927 8
Size: 352 889 1629 43
Fragments arranged by size:
1629 1425 889 725 498 352 275 54
50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3053 3409 1537
Size: 275 1241 356 4090
Fragments arranged by size:
4090 1241 356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3892 1353
Size: 2539 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2891 3162 4025 4622 5409 5677
Size: 393 487 772 271 863 597 787 268
Cuts at: 5677 1239
Size: 1524
Fragments arranged by size:
1524 863 787 772 597 487 393 271
268
EcoO109I rG'GnC_Cy
Cuts at: 2119 2119
Size: 5962
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FseI GG_CCGG'CC
Cuts at: 3059 3059
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2483 2515 2593
Size: 32 135 32 449 32 430 32 78
Cuts at: 2593 2625 2861 2893 4603 4635 4677 4709 1373
Size: 32 236 32 1710 32 42 32 2626
Fragments arranged by size:
2626 1710 449 430 236 135 78 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2556 748
Size: 1808 4154
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 5076 5260 1305
Size: 810 2961 184 2007
Fragments arranged by size:
2961 2007 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 2503 2895 2986 3920 4279 4438
Size: 648 1296 41 392 91 934 359 159
Cuts at: 4438 518
Size: 2042
Fragments arranged by size:
2042 1296 934 648 392 359 159 91
41
MspA1I CmG'CkG
Cuts at: 1606 2022 2080 2282 2842 3378 4055 5203 5448
Size: 416 58 202 560 536 677 1148 245
Cuts at: 5448 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 560 536 416 245 202
58
NaeI GCC'GGC
Cuts at: 3057 3057
Size: 5962
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NgoMIV G'CCGG_C
Cuts at: 3055 3055
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2467 3091 3320 4865 1132
Size: 97 1238 624 229 1545 2229
Fragments arranged by size:
2229 1545 1238 624 229 97
PasI CC'CwG_GG
Cuts at: 1348 3043 1348
Size: 1695 4267
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2923 1108
Size: 1815 4147
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2119
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 2241 1613
Size: 628 5334
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2256 2718 2725 2805 3314 830
Size: 1426 462 7 80 509 3478
Fragments arranged by size:
3478 1426 509 462 80 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2080 2842 2080
Size: 762 5200
SacII CC_GC'GG
Cuts at: 2283 2283
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 2805 3314 2805
Size: 509 5453
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2252 2714 2721 2801 3310 4445 5126
Size: 219 1207 462 7 80 509 1135 681
Cuts at: 5126 5317 826
Size: 191 1471
Fragments arranged by size:
1471 1207 1135 681 509 462 219 191
80 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4066 4967 5229 820
Size: 197 926 2123 901 262 1553
Fragments arranged by size:
2123 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 3401 513
Size: 552 2336 3074
Fragments arranged by size:
3074 2336 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2349 3989 4006 4159 4344 4789 325
Size: 1255 769 1640 17 153 185 445 1498
Fragments arranged by size:
1640 1498 1255 769 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4208 5920 96
Size: 274 80 33 51 1443 2231 1712 138
Fragments arranged by size:
2231 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2478 2510 5922 5954 2478
Size: 32 3412 32 2486
Fragments arranged by size:
3412 2486 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2047
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 3025 4091 3025
Size: 1066 4896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI