Restriction map for
pFN21AB7706
(Circular) (Six-base) MAPSORT of: pFN21AB7706.seq Check: 5660 from: 1 to: 6100
pFN21AB7706 6100 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2012 3910 278
Size: 53 83 186 936 476 1898 2468
Fragments arranged by size:
2468 1898 936 476 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2829 3443 1937
Size: 892 614 4594
Fragments arranged by size:
4594 892 614
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3320 4163 5547 5815 1632
Size: 1688 843 1384 268 1917
Fragments arranged by size:
1917 1688 1384 843 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 4829 4829
Size: 6100
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6100
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2988 3020 2988
Size: 32 6068
AlwNI CAG_nnn'CTG
Cuts at: 3135 3178 3343 3843 5415 3135
Size: 43 165 500 1572 3820
Fragments arranged by size:
3820 1572 500 165 43
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 2336 2441 3147 3409 3635 3787 5896 1493
Size: 843 105 706 262 226 152 2109 1697
Fragments arranged by size:
2109 1697 843 706 262 226 152 105
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 3425 1870
Size: 73 1482 4545
Fragments arranged by size:
4545 1482 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 3421 4876 618
Size: 325 217 627 357 1277 1455 1842
Fragments arranged by size:
1842 1455 1277 627 357 325 217
BanII G_rGCy'C
Cuts at: 1391 3222 3371 3419 1391
Size: 1831 149 48 4072
Fragments arranged by size:
4072 1831 149 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4291 4325 1903
Size: 34 2354 34 3678
Fragments arranged by size:
3678 2354 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2722 3992 5202 2722
Size: 1270 1210 3620
Fragments arranged by size:
3620 1270 1210
BclI T'GATC_A
Cuts at: 1548 2695 1548
Size: 1147 4953
BfrBI ATG'CAT
Cuts at: 2870 2870
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4711 136
Size: 107 122 71 631 605 3039 1525
Fragments arranged by size:
3039 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2213 6095 2213
Size: 3882 2218
BlpI GC'TnA_GC
Cuts at: 3171 3517 3171
Size: 346 5754
Bme1580I G_kGCm'C
Cuts at: 1790 4161 5317 1790
Size: 2371 1156 2573
Fragments arranged by size:
2573 2371 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2757 3831 4789
Size: 416 53 83 399 24 1340 1074 958
Cuts at: 4789 442
Size: 1753
Fragments arranged by size:
1753 1340 1074 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2601 1056
Size: 1545 4555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2400 4760 1239
Size: 941 220 2360 2579
Fragments arranged by size:
2579 2360 941 220
Bpu10I CC'TnA_GC
Cuts at: 2483 2483
Size: 6100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 4225 5090 5388 2191
Size: 2034 865 298 2903
Fragments arranged by size:
2903 2034 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4763 882
Size: 617 3264 2219
Fragments arranged by size:
3264 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2227 3907 4289
Size: 53 83 186 936 476 218 1680 382
Cuts at: 4289 4924 275
Size: 635 1451
Fragments arranged by size:
1680 1451 936 635 476 382 218 186
83 53
BsaWI w'CCGG_w
Cuts at: 4533 4931 5205 5352 5763 4533
Size: 398 274 147 411 4870
Fragments arranged by size:
4870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2656 2686 1178
Size: 30 165 30 1253 30 4592
Fragments arranged by size:
4592 1253 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2099 2661 1186
Size: 910 3 562 4625
Fragments arranged by size:
4625 910 562 3
BseYI C'CCAG_C
Cuts at: 2620 2656 2718 3128 5303 5799 2620
Size: 36 62 410 2175 496 2921
Fragments arranged by size:
2921 2175 496 410 62 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5527
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3222 4161 4246 5317 3222
Size: 939 85 1071 4005
Fragments arranged by size:
4005 1071 939 85
BsmI GAATG_Cn'
Cuts at: 3047 3611 3704 3047
Size: 564 93 5443
Fragments arranged by size:
5443 564 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3222 3371 3419 4161 4246 5317 1391
Size: 399 1432 149 48 742 85 1071 2174
Fragments arranged by size:
2174 1432 1071 742 399 149 85 48
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 2308 3989 5719 2308
Size: 1681 1730 2689
Fragments arranged by size:
2689 1730 1681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2224 3455 844
Size: 1380 1231 3489
Fragments arranged by size:
3489 1380 1231
BsrBI CCG'CTC
Cuts at: 1394 1823 3987 1394
Size: 429 2164 3507
Fragments arranged by size:
3507 2164 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4595 4769 65
Size: 1183 3347 174 1396
Fragments arranged by size:
3347 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3279 4744 4931 1700
Size: 110 1469 1465 187 2869
Fragments arranged by size:
2869 1469 1465 187 110
BsrGI T'GTAC_A
Cuts at: 96 3067 96
Size: 2971 3129
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2111 794
Size: 1317 4783
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6100
BstYI r'GATC_y
Cuts at: 1972 2213 2348 2905 3430 3797 4182 4199 5651
Size: 241 135 557 525 367 385 17 1452
Cuts at: 5651 5737 5749 6095 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 557 525 385 367 346 241
135 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2148 3196 513
Size: 552 1083 1048 3417
Fragments arranged by size:
3417 1083 1048 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5361
Fragments arranged by size:
5361 683 56
BtsI GCAGTG_nn'
Cuts at: 3616 4410 4430 3616
Size: 794 20 5286
Fragments arranged by size:
5286 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2640 5107 809
Size: 507 1324 2467 1802
Fragments arranged by size:
2467 1802 1324 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2288 3547
Size: 54 1425 50 275 18 186 272 1259
Cuts at: 3547 4436 6065 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1259 889 275 272 186 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2796 3339 4030 1353
Size: 1443 543 691 3423
Fragments arranged by size:
3423 1443 691 543
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4687 5061 5207 387
Size: 1064 138 3098 374 146 1280
Fragments arranged by size:
3098 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3220 3220
Size: 6100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2400 3320 4163 4760 5547 5815
Size: 393 548 220 920 843 597 787 268
Cuts at: 5815 1239
Size: 1524
Fragments arranged by size:
1524 920 843 787 597 548 393 268
220
EcoRI G'AATT_C
Cuts at: 2441 3409 3787 2441
Size: 968 378 4754
Fragments arranged by size:
4754 968 378
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 2287 5247 1678
Size: 609 2960 2531
Fragments arranged by size:
2960 2531 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4741 4773 4815 4847 1373
Size: 32 135 32 3169 32 42 32 2626
Fragments arranged by size:
3169 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3193 3444 3690 669
Size: 988 281 1255 251 246 3079
Fragments arranged by size:
3079 1255 988 281 251 246
HindIII A'AGCT_T
Cuts at: 748 2859 748
Size: 2111 3989
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 2970 3699 2970
Size: 729 5371
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2304 2833 3287 5214 5398 1305
Size: 999 529 454 1927 184 2007
Fragments arranged by size:
2007 1927 999 529 454 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2018 10
Size: 54 1425 343 186 4092
Fragments arranged by size:
4092 1425 343 186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3227 3242 3324 4058 4417 4576 518
Size: 648 2061 15 82 734 359 159 2042
Fragments arranged by size:
2061 2042 734 648 359 159 82 15
MspA1I CmG'CkG
Cuts at: 1606 2660 3135 3516 4193 5341 5586 1606
Size: 1054 475 381 677 1148 245 2120
Fragments arranged by size:
2120 1148 1054 677 475 381 245
NcoI C'CATG_G
Cuts at: 513 1065 2148 3196 513
Size: 552 1083 1048 3417
Fragments arranged by size:
3417 1083 1048 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NheI G'CTAG_C
Cuts at: 1052 2597 1052
Size: 1545 4555
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NsiI A_TGCA'T
Cuts at: 2872 2872
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 2506 3458 5003 1132
Size: 97 1277 952 1545 2229
Fragments arranged by size:
2229 1545 1277 952 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6100
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2725 1108
Size: 1617 4483
PfoI T'CCnGG_A
Cuts at: 2179 2179
Size: 6100
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PstI C_TGCA'G
Cuts at: 830 2235 3140 3452 830
Size: 1405 905 312 3478
Fragments arranged by size:
3478 1405 905 312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 2660 3135 2660
Size: 475 5625
SacI G_AGCT'C
Cuts at: 3222 3222
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SapI GCTCTTCn'nnn_
Cuts at: 2796 2796
Size: 6100
SbfI CC_TGCA'GG
Cuts at: 2235 3452 2235
Size: 1217 4883
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SfcI C'TryA_G
Cuts at: 826 1045 2231 3136 3448 4583 5264 5455 826
Size: 219 1186 905 312 1135 681 191 1471
Fragments arranged by size:
1471 1186 1135 905 681 312 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 3427 1872
Size: 1555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2170 4204 5105 5367 820
Size: 197 926 227 2034 901 262 1553
Fragments arranged by size:
2034 1553 926 901 262 227 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 2036 2879 152
Size: 1884 843 3373
Fragments arranged by size:
3373 1884 843
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6100
StyI C'CwwG_G
Cuts at: 513 1065 2148 3196 3539 513
Size: 552 1083 1048 343 3074
Fragments arranged by size:
3074 1083 1048 552 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4127 4144 4297 4482 4927 325
Size: 1255 2547 17 153 185 445 1498
Fragments arranged by size:
2547 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3012 3067 4346
Size: 274 80 33 51 1443 1035 55 1279
Cuts at: 4346 6058 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1279 1035 274 138 80 55
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2059 2091 2542 2574 6060 6092 2059
Size: 32 451 32 3486 32 2067
Fragments arranged by size:
3486 2067 451 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2155 2011
Size: 144 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 3425 1870
Size: 1555 4545
XmnI GAAnn'nnTTC
Cuts at: 2862 4229 2862
Size: 1367 4733
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2010 3908 276
Size: 53 83 186 936 476 1898 2468
Fragments arranged by size:
2468 1898 936 476 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI