Restriction map for
pFN21AB7705
(Circular) (Six-base) MAPSORT of: pFN21AB7705.seq Check: 3443 from: 1 to: 5962
pFN21AB7705 5962 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2217 3305 1937
Size: 280 1088 4594
Fragments arranged by size:
4594 1088 280
Acc65I G'GTAC_C
Cuts at: 2494 3283 2494
Size: 789 5173
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 4025 5409 5677 1632
Size: 482 1911 1384 268 1917
Fragments arranged by size:
1917 1911 1384 482 268
AflII C'TTAA_G
Cuts at: 820 1017 2249 2521 2599 820
Size: 197 1232 272 78 4183
Fragments arranged by size:
4183 1232 272 197 78
AflIII A'CryG_T
Cuts at: 1203 3259 4861 5838 1203
Size: 2056 1602 977 1327
Fragments arranged by size:
2056 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 2319 1166
Size: 1153 4809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 3091 3705 5277 3091
Size: 614 1572 3776
Fragments arranged by size:
3776 1572 614
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 3287 1870
Size: 73 1344 4545
Fragments arranged by size:
4545 1344 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2494 3283 4738 618
Size: 325 217 627 707 789 1455 1842
Fragments arranged by size:
1842 1455 789 707 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2927 3281 1391
Size: 1536 354 4072
Fragments arranged by size:
4072 1536 354
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2508 928
Size: 971 609 4382
Fragments arranged by size:
4382 971 609
BbvCI CC'TCA_GC
Cuts at: 3084 3084
Size: 5962
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 2152 2167 1548
Size: 604 15 5343
Fragments arranged by size:
5343 604 15
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 3013 4023 5179 1790
Size: 1223 1010 1156 2573
Fragments arranged by size:
2573 1223 1156 1010
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2946 2946
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2424 4622 1239
Size: 1185 2198 2579
Fragments arranged by size:
2579 2198 1185
Bpu10I CC'TnA_GC
Cuts at: 3084 3084
Size: 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4087 4952 5250 4087
Size: 865 298 4799
Fragments arranged by size:
4799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4625 882
Size: 617 3126 2219
Fragments arranged by size:
3126 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2080 3753 493
Size: 711 876 1673 2702
Fragments arranged by size:
2702 1673 876 711
BsaBI GATnn'nnATC
Cuts at: 2151 5956 2151
Size: 3805 2157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3007 3037 1178
Size: 30 165 30 1604 30 4103
Fragments arranged by size:
4103 1604 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2917 1186
Size: 1731 4231
BseYI C'CCAG_C
Cuts at: 3026 3055 3061 5165 5661 3026
Size: 29 6 2104 496 3327
Fragments arranged by size:
3327 2104 496 29 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3077 1163
Size: 573 1341 4048
Fragments arranged by size:
4048 1341 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2590 2927 3023 4023 4108 5179 2590
Size: 337 96 1000 85 1071 3373
Fragments arranged by size:
3373 1071 1000 337 96 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2590 2927 3013 3023 3281 4023 4108
Size: 399 800 337 86 10 258 742 85
Cuts at: 4108 5179 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 800 742 399 337 258 86
85 10
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2862 3851 5581 2862
Size: 989 1730 3243
Fragments arranged by size:
3243 1730 989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2978 3317 844
Size: 2134 339 3489
Fragments arranged by size:
3489 2134 339
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3070 4606 4793 1700
Size: 110 1260 1536 187 2869
Fragments arranged by size:
2869 1536 1260 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 3016 4022 5034 3016
Size: 1006 1012 3944
Fragments arranged by size:
3944 1012 1006
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957
Size: 1320 367 385 17 1452 86 12 346
Cuts at: 5957 1972
Size: 1977
Fragments arranged by size:
1977 1452 1320 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2613 513
Size: 552 939 609 3862
Fragments arranged by size:
3862 939 609 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2452 3004 3097 3478 4272 4292 2452
Size: 552 93 381 794 20 4122
Fragments arranged by size:
4122 794 552 381 93 20
ClaI AT'CG_AT
Cuts at: 1554 2335 1554
Size: 781 5181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2951 3409 4298
Size: 54 1425 50 275 18 1121 458 889
Cuts at: 4298 5927 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1121 889 458 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2578 3892 1353
Size: 780 445 1314 3423
Fragments arranged by size:
3423 1314 780 445
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2925 2925
Size: 5962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2424 4025 4622 5409 5677 1239
Size: 393 482 310 1601 597 787 268 1524
Fragments arranged by size:
1601 1524 787 597 482 393 310 268
EcoO109I rG'GnC_Cy
Cuts at: 2232 2728 3079 2232
Size: 496 351 5115
Fragments arranged by size:
5115 496 351
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2625 2625
Size: 5962
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2186 2191 2218 2577
Size: 32 135 32 587 27 5 27 359
Cuts at: 2577 2609 4603 4635 4677 4709 1373
Size: 32 1994 32 42 32 2626
Fragments arranged by size:
2626 1994 587 359 135 42 32 32
32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2246 748
Size: 1498 4464
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 2498 3287 2498
Size: 789 5173
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5076 5260 1305
Size: 3771 184 2007
Fragments arranged by size:
3771 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2319 2419 3165 3920 4279 4438 518
Size: 648 1153 100 746 755 359 159 2042
Fragments arranged by size:
2042 1153 755 746 648 359 159 100
MspA1I CmG'CkG
Cuts at: 1606 2442 3378 4055 5203 5448 1606
Size: 836 936 677 1148 245 2120
Fragments arranged by size:
2120 1148 936 836 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2613 513
Size: 552 939 609 3862
Fragments arranged by size:
3862 939 609 552
NdeI CA'TA_TG
Cuts at: 387 2388 3150 387
Size: 2001 762 3199
Fragments arranged by size:
3199 2001 762
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 3263 3320 4865 1132
Size: 97 2034 57 1545 2229
Fragments arranged by size:
2229 2034 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 3259 4861 3259
Size: 1602 4360
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2714 1108
Size: 1606 4356
PfoI T'CCnGG_A
Cuts at: 3075 3075
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PmlI CAC'GTG
Cuts at: 2080 2080
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2232 2728 3079 2232
Size: 496 351 5115
Fragments arranged by size:
5115 496 351
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2447 3314 830
Size: 1617 867 3478
Fragments arranged by size:
3478 1617 867
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
SacI G_AGCT'C
Cuts at: 2927 2927
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SanDI GG'GwC_CC
Cuts at: 2728 2728
Size: 5962
SapI GCTCTTCn'nnn_
Cuts at: 2133 2578 2133
Size: 445 5517
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2443 2575 2974 3310 4445 5126 5317
Size: 219 1398 132 399 336 1135 681 191
Cuts at: 5317 826
Size: 1471
Fragments arranged by size:
1471 1398 1135 681 399 336 219 191
132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 2521 2599 4066 4967 5229
Size: 197 926 306 272 78 1467 901 262
Cuts at: 5229 820
Size: 1553
Fragments arranged by size:
1553 1467 926 901 306 272 262 197
78
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2190 2613 2723 3401 513
Size: 552 939 186 423 110 678 3074
Fragments arranged by size:
3074 939 678 552 423 186 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2187 3989 4006 4159 4344 4789
Size: 1255 417 190 1802 17 153 185 445
Cuts at: 4789 325
Size: 1498
Fragments arranged by size:
1802 1498 1255 445 417 190 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3117 4208 5920
Size: 274 80 33 51 1443 1140 1091 1712
Cuts at: 5920 96
Size: 138
Fragments arranged by size:
1712 1443 1140 1091 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3007 3039 5922 5954 3007
Size: 32 2883 32 3015
Fragments arranged by size:
3015 2883 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII
SexAI SfiI SfoI SrfI SwaI XcmI