Restriction map for
pFN21AB7703
(Circular) (Six-base) MAPSORT of: pFN21AB7703.seq Check: 2186 from: 1 to: 5953
pFN21AB7703 5953 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3036 3036
Size: 5953
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2403 3763 278
Size: 53 83 186 936 867 1360 2468
Fragments arranged by size:
2468 1360 936 867 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3296 1937
Size: 1359 4594
Acc65I G'GTAC_C
Cuts at: 2803 3274 2803
Size: 471 5482
AclI AA'CG_TT
Cuts at: 1563 3252 4080 4453 1563
Size: 1689 828 373 3063
Fragments arranged by size:
3063 1689 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2145 4016 5400 5668 1632
Size: 513 1871 1384 268 1917
Fragments arranged by size:
1917 1871 1384 513 268
AfeI AGC'GCT
Cuts at: 2576 2576
Size: 5953
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 4852 5829 1203
Size: 3649 977 1327
Fragments arranged by size:
3649 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 3106 4682 3106
Size: 1576 4377
AleI CACnn'nnGTG
Cuts at: 1166 3042 3132 1166
Size: 1876 90 3987
Fragments arranged by size:
3987 1876 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5953
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 5921
AlwNI CAG_nnn'CTG
Cuts at: 2151 2711 2758 3696 5268 2151
Size: 560 47 938 1572 2836
Fragments arranged by size:
2836 1572 938 560 47
ApaI G_GGCC'C
Cuts at: 2325 2624 2636 2325
Size: 299 12 5642
Fragments arranged by size:
5642 299 12
ApaLI G'TGCA_C
Cuts at: 3209 4010 5166 3209
Size: 801 1156 3996
Fragments arranged by size:
3996 1156 801
ApoI r'AATT_y
Cuts at: 1493 3262 3488 3640 5749 1493
Size: 1769 226 152 2109 1697
Fragments arranged by size:
2109 1769 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 2372 3278 1870
Size: 73 429 906 4545
Fragments arranged by size:
4545 906 429 73
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2210 2597 2612 2803 3274
Size: 325 217 627 423 387 15 191 471
Cuts at: 3274 4729 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 471 423 387 325 217
191 15
BanII G_rGCy'C
Cuts at: 1391 2325 2624 2636 3272 1391
Size: 934 299 12 636 4072
Fragments arranged by size:
4072 934 636 299 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4982
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4144 4178 1903
Size: 34 2207 34 3678
Fragments arranged by size:
3678 2207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4564 136
Size: 107 122 71 631 605 2892 1525
Fragments arranged by size:
2892 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5948 5948
Size: 5953
BlpI GC'TnA_GC
Cuts at: 3370 3370
Size: 5953
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2325 2617 2624 2636 2705 2796 2859
Size: 423 112 292 7 12 69 91 63
Cuts at: 2859 3213 4014 5170 1790
Size: 354 801 1156 2573
Fragments arranged by size:
2573 1156 801 423 354 292 112 91
69 63 12 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5953
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2051 2651 3684
Size: 416 53 83 399 24 634 600 1033
Cuts at: 3684 4642 442
Size: 958 1753
Fragments arranged by size:
1753 1033 958 634 600 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2056 2056
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2128 2447 2468 2487 3103 4613 1239
Size: 889 319 21 19 616 1510 2579
Fragments arranged by size:
2579 1510 889 616 319 21 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2160 3002 4078 4943 5241 2160
Size: 842 1076 865 298 2872
Fragments arranged by size:
2872 1076 865 842 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2915 4616 882
Size: 617 1416 1701 2219
Fragments arranged by size:
2219 1701 1416 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 493
Size: 711 2540 2702
Fragments arranged by size:
2702 2540 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2400 3760 4142 4777
Size: 53 83 186 936 867 1360 382 635
Cuts at: 4777 275
Size: 1451
Fragments arranged by size:
1451 1360 936 867 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2813 2843 3176 3206 1178
Size: 30 165 30 1410 30 333 30 3925
Fragments arranged by size:
3925 1410 333 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2465 2800 2903 1186
Size: 1258 21 335 103 4236
Fragments arranged by size:
4236 1258 335 103 21
BseYI C'CCAG_C
Cuts at: 2260 2528 2751 2941 5156 5652 2260
Size: 268 223 190 2215 496 2561
Fragments arranged by size:
2561 2215 496 268 223 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2625 3046 1163
Size: 573 889 421 4070
Fragments arranged by size:
4070 889 573 421
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3403 4164 5192 1540
Size: 275 186 1402 761 1028 2301
Fragments arranged by size:
2301 1402 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3213 4014 4099 5170 3213
Size: 801 85 1071 3996
Fragments arranged by size:
3996 1071 801 85
BsmI GAATG_Cn'
Cuts at: 3464 3557 3464
Size: 93 5860
BsmBI CGTCTCn'nnnn_
Cuts at: 2125 2125
Size: 5953
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2325 2617 2624 2636 2705 2796
Size: 399 423 112 292 7 12 69 91
Cuts at: 2796 2859 3213 3272 4014 4099 5170 1391
Size: 63 354 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 423 399 354 292 112
91 85 69 63 59 12 7
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2832 3036 3308 844
Size: 1988 204 272 3489
Fragments arranged by size:
3489 1988 272 204
BsrBI CCG'CTC
Cuts at: 1394 1823 3234 3840 1394
Size: 429 1411 606 3507
Fragments arranged by size:
3507 1411 606 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2844 2859 4448 4622 65
Size: 1183 1596 15 1589 174 1396
Fragments arranged by size:
1596 1589 1396 1183 174 15
BsrFI r'CCGG_y
Cuts at: 1700 1810 2088 2906 3033 4597 4784 1700
Size: 110 278 818 127 1564 187 2869
Fragments arranged by size:
2869 1564 818 278 187 127 110
BsrGI T'GTAC_A
Cuts at: 96 2997 96
Size: 2901 3052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 2789 4013 5025 2789
Size: 1224 1012 3717
Fragments arranged by size:
3717 1224 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2612 794
Size: 1818 4135
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 2670 3283 3650 4035 4052 5504 5590 5602
Size: 698 613 367 385 17 1452 86 12
Cuts at: 5602 5948 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 698 613 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2484 2484
Size: 5953
BtgI C'CryG_G
Cuts at: 513 1065 2004 2310 2405 3221 513
Size: 552 939 306 95 816 3245
Fragments arranged by size:
3245 939 816 552 306 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2151 487
Size: 683 56 925 4289
Fragments arranged by size:
4289 925 683 56
BtsI GCAGTG_nn'
Cuts at: 2272 2308 3469 4263 4283 2272
Size: 36 1161 794 20 3942
Fragments arranged by size:
3942 1161 794 36 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DraIII CAC_nnn'GTG
Cuts at: 2594 2594
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 3068 3400
Size: 54 1425 50 275 18 256 982 332
Cuts at: 3400 4289 5918 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 982 889 332 275 256 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 3883 1353
Size: 1080 1450 3423
Fragments arranged by size:
3423 1450 1080
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4540 4914 5060 387
Size: 1064 138 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 2145 2447 2468 2487 3103 4016
Size: 393 496 17 302 21 19 616 913
Cuts at: 4016 4613 5400 5668 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 913 787 616 597 496 393 302
268 21 19 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2329 2589 2329
Size: 260 5693
EcoO109I rG'GnC_Cy
Cuts at: 2321 2322 2479 2620 2632 2633 2708 2885 2894
Size: 1 157 141 12 1 75 177 9
Cuts at: 2894 2321
Size: 5380
Fragments arranged by size:
5380 177 157 141 75 12 9 1
1
EcoRI G'AATT_C
Cuts at: 3262 3640 3262
Size: 378 5575
FspI TGC'GCA
Cuts at: 2843 4459 2843
Size: 1616 4337
FspAI rTGC'GCAy
Cuts at: 2843 2843
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 2578 3124 5100 1678
Size: 900 546 1976 2531
Fragments arranged by size:
2531 1976 900 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3092 3124 3176 3208 4594
Size: 32 135 32 1520 32 52 32 1386
Cuts at: 4594 4626 4668 4700 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1520 1386 135 52 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2764 3297 3543 669
Size: 988 281 826 533 246 3079
Fragments arranged by size:
3079 988 826 533 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5953
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KpnI G_GTAC'C
Cuts at: 2807 3278 2807
Size: 471 5482
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2838 2857 5067 5251 1305
Size: 1533 19 2210 184 2007
Fragments arranged by size:
2210 2007 1533 184 19
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3070 10
Size: 54 1425 343 1238 2893
Fragments arranged by size:
2893 1425 1238 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2657 2960 3042 3132 3206 3911 4270
Size: 648 1491 303 82 90 74 705 359
Cuts at: 4270 4429 518
Size: 159 2042
Fragments arranged by size:
2042 1491 705 648 359 303 159 90
82 74
MspA1I CmG'CkG
Cuts at: 1606 2280 2339 2528 2565 3223 3369 4046 5194
Size: 674 59 189 37 658 146 677 1148
Cuts at: 5194 5439 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 674 658 245 189 146
59 37
NaeI GCC'GGC
Cuts at: 2090 3035 2090
Size: 945 5008
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4462
Fragments arranged by size:
4462 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5953
NgoMIV G'CCGG_C
Cuts at: 2088 3033 2088
Size: 945 5008
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 2107 3311 4856 1132
Size: 97 878 1204 1545 2229
Fragments arranged by size:
2229 1545 1204 878 97
PasI CC'CwG_GG
Cuts at: 1348 2617 2720 2890 1348
Size: 1269 103 170 4411
Fragments arranged by size:
4411 1269 170 103
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2594 2758 1108
Size: 1486 164 4303
Fragments arranged by size:
4303 1486 164
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2845 3155 3187 4022 4054 5535 5567 2813
Size: 32 310 32 835 32 1481 32 3199
Fragments arranged by size:
3199 1481 835 310 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2885 2479
Size: 406 5547
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PspOMI G'GGCC_C
Cuts at: 2321 2620 2632 2321
Size: 299 12 5642
Fragments arranged by size:
5642 299 12
PstI C_TGCA'G
Cuts at: 830 2516 2670 2990 3305 830
Size: 1686 154 320 315 3478
Fragments arranged by size:
3478 1686 320 315 154
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
PvuII CAG'CTG
Cuts at: 2528 2528
Size: 5953
SacII CC_GC'GG
Cuts at: 3224 3224
Size: 5953
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SanDI GG'GwC_CC
Cuts at: 2885 2885
Size: 5953
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 4201 5913 4201
Size: 1712 4241
SexAI A'CCwGG_T
Cuts at: 2590 2590
Size: 5953
SfcI C'TryA_G
Cuts at: 826 1045 2512 2666 2986 3301 4436 5117 5308
Size: 219 1467 154 320 315 1135 681 191
Cuts at: 5308 826
Size: 1471
Fragments arranged by size:
1471 1467 1135 681 320 315 219 191
154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2175 3017 4057 4958 5220 820
Size: 197 926 232 842 1040 901 262 1553
Fragments arranged by size:
1553 1040 926 901 842 262 232 197
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 2205 1517
Size: 688 5265
StyI C'CwwG_G
Cuts at: 513 1065 2004 3392 513
Size: 552 939 1388 3074
Fragments arranged by size:
3074 1388 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 2375 3980 3997 4150 4335 4780
Size: 1255 480 315 1605 17 153 185 445
Cuts at: 4780 325
Size: 1498
Fragments arranged by size:
1605 1498 1255 480 445 315 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2972 2997 4199
Size: 274 80 33 51 1443 995 25 1202
Cuts at: 4199 5911 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1202 995 274 138 80 51
33 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2397 2429 5913 5945 2397
Size: 32 3484 32 2405
Fragments arranged by size:
3484 2405 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2525 2552 2624 3078 2525
Size: 27 72 454 5400
Fragments arranged by size:
5400 454 72 27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5953
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 2442 3053 3170 4082 2442
Size: 611 117 912 4313
Fragments arranged by size:
4313 912 611 117
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2401 3761 276
Size: 53 83 186 936 867 1360 2468
Fragments arranged by size:
2468 1360 936 867 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BstXI BstZ17I EcoICRI EcoRV FalI FseI KasI NarI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SapI SfiI SfoI SrfI SwaI