Restriction map for
pFN21AB7701
(Circular) (Six-base) MAPSORT of: pFN21AB7701.seq Check: 7362 from: 1 to: 5947
pFN21AB7701 5947 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2620 2620
Size: 5947
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4010 5394 5662 1632
Size: 2378 1384 268 1917
Fragments arranged by size:
2378 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 3121 4846 5823 1203
Size: 1918 1725 977 1327
Fragments arranged by size:
1918 1725 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 2123 2982 3122 1166
Size: 957 859 140 3991
Fragments arranged by size:
3991 957 859 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3140 1184
Size: 1956 3991
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2642 2674 2642
Size: 32 5915
AlwNI CAG_nnn'CTG
Cuts at: 2269 3690 5262 2269
Size: 1421 1572 2954
Fragments arranged by size:
2954 1572 1421
ApaLI G'TGCA_C
Cuts at: 2294 3067 4004 5160 2294
Size: 773 937 1156 3081
Fragments arranged by size:
3081 1156 937 773
ApoI r'AATT_y
Cuts at: 1493 2837 3256 3482 3634 5743 1493
Size: 1344 419 226 152 2109 1697
Fragments arranged by size:
2109 1697 1344 419 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 2602 3272 1870
Size: 73 68 591 670 4545
Fragments arranged by size:
4545 670 591 73 68
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 2594 3268 4723 618
Size: 325 217 627 620 187 674 1455 1842
Fragments arranged by size:
1842 1455 674 627 620 325 217 187
BanII G_rGCy'C
Cuts at: 1391 3266 1391
Size: 1875 4072
BbeI G_GCGC'C
Cuts at: 2598 2598
Size: 5947
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4976
BbvCI CC'TCA_GC
Cuts at: 2561 2561
Size: 5947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2275 2309 4138 4172 1903
Size: 34 338 34 1829 34 3678
Fragments arranged by size:
3678 1829 338 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2579 4558 136
Size: 107 122 71 631 605 907 1979 1525
Fragments arranged by size:
1979 1525 907 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 2205 2298 2412 3071 3161 4008 5164 1790
Size: 415 93 114 659 90 847 1156 2573
Fragments arranged by size:
2573 1156 847 659 415 114 93 90
BmgBI CAC'GTC
Cuts at: 1318 2980 1318
Size: 1662 4285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3678 4636 442
Size: 416 53 83 399 24 2261 958 1753
Fragments arranged by size:
2261 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 2811 3069 4607 1239
Size: 1430 142 258 1538 2579
Fragments arranged by size:
2579 1538 1430 258 142
Bpu10I CC'TnA_GC
Cuts at: 2561 3228 2561
Size: 667 5280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4072 4937 5235 4072
Size: 865 298 4784
Fragments arranged by size:
4784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 2099 2108 4610 882
Size: 617 585 15 9 2502 2219
Fragments arranged by size:
2502 2219 617 585 15 9
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 2399 5941 2399
Size: 3542 2405
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2595 3754 4136 4771
Size: 53 83 186 936 1062 1159 382 635
Cuts at: 4771 275
Size: 1451
Fragments arranged by size:
1451 1159 1062 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3137 4380 4778 5052 5199 5610 3137
Size: 1243 398 274 147 411 3474
Fragments arranged by size:
3474 1243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2642 2672 1178
Size: 30 165 30 1239 30 4453
Fragments arranged by size:
4453 1239 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2150 2153 2156 2348 1186
Size: 961 3 3 3 192 4785
Fragments arranged by size:
4785 961 192 3 3 3
BseYI C'CCAG_C
Cuts at: 5150 5646 5150
Size: 496 5451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 2342 2654 2896 2939 3006 1163
Size: 573 321 285 312 242 43 67 4104
Fragments arranged by size:
4104 573 321 312 285 242 67 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2421 2472 2515 3397 4158
Size: 275 186 57 363 51 43 882 761
Cuts at: 4158 5186 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 882 761 363 275 186 57
51 43
BsiHKAI G_wGCw'C
Cuts at: 2298 3071 4008 4093 5164 2298
Size: 773 937 85 1071 3081
Fragments arranged by size:
3081 1071 937 773 85
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2298 2412 3071 3161 3266 4008
Size: 399 415 93 114 659 90 105 742
Cuts at: 4008 4093 5164 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 659 415 399 114 105
93 90 85
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2620 3302 844
Size: 1776 682 3489
Fragments arranged by size:
3489 1776 682
BsrBI CCG'CTC
Cuts at: 1394 1823 2481 2544 3834 1394
Size: 429 658 63 1290 3507
Fragments arranged by size:
3507 1290 658 429 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 2207 2416 2420 2471 2573 4591
Size: 110 243 154 209 4 51 102 2018
Cuts at: 4591 4778 1700
Size: 187 2869
Fragments arranged by size:
2869 2018 243 209 187 154 110 102
51 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 2368 1925
Size: 443 5504
BssSI C'ACGA_G
Cuts at: 2075 4007 5019 2075
Size: 1932 1012 3003
Fragments arranged by size:
3003 1932 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2840 794
Size: 2046 3901
BstBI TT'CG_AA
Cuts at: 3075 4751 3075
Size: 1676 4271
BstEII G'GTnAC_C
Cuts at: 1184 2272 2455 2665 1184
Size: 1088 183 210 4466
Fragments arranged by size:
4466 1088 210 183
BstXI CCAn_nnnn'nTGG
Cuts at: 2101 2125 2830 2101
Size: 24 705 5218
Fragments arranged by size:
5218 705 24
BstYI r'GATC_y
Cuts at: 1972 2818 3277 3644 4029 4046 5498 5584 5596
Size: 846 459 367 385 17 1452 86 12
Cuts at: 5596 5942 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 846 459 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2179 2197 2460 2962 513
Size: 552 939 30 145 18 263 502 3498
Fragments arranged by size:
3498 939 552 502 263 145 30 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2014 487
Size: 683 56 788 4420
Fragments arranged by size:
4420 788 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 4257 4277 3463
Size: 794 20 5133
Fragments arranged by size:
5133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DraIII CAC_nnn'GTG
Cuts at: 2673 2673
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2194 2209
Size: 54 1425 50 275 18 225 139 15
Cuts at: 2209 2418 2469 2512 2937 3394 4283 5912 8
Size: 209 51 43 425 457 889 1629 43
Fragments arranged by size:
1629 1425 889 457 425 275 225 209
139 54 51 50 43 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2418 2469 2512 3394 1537
Size: 275 243 363 51 43 882 4090
Fragments arranged by size:
4090 882 363 275 243 51 43
EarI CTCTTCn'nnn_
Cuts at: 1353 2534 2546 3877 1353
Size: 1181 12 1331 3423
Fragments arranged by size:
3423 1331 1181 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2669 2811 3069 4010 4607 5394 5662
Size: 393 1037 142 258 941 597 787 268
Cuts at: 5662 1239
Size: 1524
Fragments arranged by size:
1524 1037 941 787 597 393 268 258
142
EcoNI CCTnn'n_nnAGG
Cuts at: 2790 2904 2790
Size: 114 5833
EcoO109I rG'GnC_Cy
Cuts at: 3147 3147
Size: 5947
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FspI TGC'GCA
Cuts at: 3116 4453 3116
Size: 1337 4610
HaeII r_GCGC'y
Cuts at: 1678 2598 5094 1678
Size: 920 2496 2531
Fragments arranged by size:
2531 2496 920
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2935 2964 2967 2996 4588
Size: 32 135 32 1363 29 3 29 1592
Cuts at: 4588 4620 4662 4694 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1592 1363 135 42 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3291 3537 669
Size: 988 281 1257 96 246 3079
Fragments arranged by size:
3079 1257 988 281 246 96
HindIII A'AGCT_T
Cuts at: 748 3000 748
Size: 2252 3695
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KasI G'GCGC_C
Cuts at: 2594 2594
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2123 2405 2918 2982 3122 3905
Size: 648 933 24 282 513 64 140 783
Cuts at: 3905 4264 4423 518
Size: 359 159 2042
Fragments arranged by size:
2042 933 783 648 513 359 282 159
140 64 24
MspA1I CmG'CkG
Cuts at: 1606 2269 2565 3363 4040 5188 5433 1606
Size: 663 296 798 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 677 663 296 245
NaeI GCC'GGC
Cuts at: 2055 2209 2418 2422 2473 2575 2055
Size: 154 209 4 51 102 5427
Fragments arranged by size:
5427 209 154 102 51 4
NarI GG'CG_CC
Cuts at: 2595 2595
Size: 5947
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4456
Fragments arranged by size:
4456 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NgoMIV G'CCGG_C
Cuts at: 2053 2207 2416 2420 2471 2573 2053
Size: 154 209 4 51 102 5427
Fragments arranged by size:
5427 209 154 102 51 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 2512 3394 2512
Size: 882 5065
NspI r_CATG'y
Cuts at: 1132 1229 3125 3227 3305 4850 1132
Size: 97 1896 102 78 1545 2229
Fragments arranged by size:
2229 1896 1545 102 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 3121 4846 3121
Size: 1725 4222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PfoI T'CCnGG_A
Cuts at: 2788 2788
Size: 5947
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2642 2674 4016 4048 5529 5561 2642
Size: 32 1342 32 1481 32 3028
Fragments arranged by size:
3028 1481 1342 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PstI C_TGCA'G
Cuts at: 830 2265 3299 830
Size: 1435 1034 3478
Fragments arranged by size:
3478 1435 1034
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SapI GCTCTTCn'nnn_
Cuts at: 2534 2534
Size: 5947
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SexAI A'CCwGG_T
Cuts at: 3243 3243
Size: 5947
SfcI C'TryA_G
Cuts at: 826 1045 2261 3295 4430 5111 5302 826
Size: 219 1216 1034 1135 681 191 1471
Fragments arranged by size:
1471 1216 1135 1034 681 219 191
SfoI GGC'GCC
Cuts at: 2596 2596
Size: 5947
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 2604 3274 1872
Size: 732 670 4545
Fragments arranged by size:
4545 732 670
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4051 4952 5214 820
Size: 197 926 2108 901 262 1553
Fragments arranged by size:
2108 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 2004 3386 513
Size: 552 939 1382 3074
Fragments arranged by size:
3074 1382 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2653 2975 3974 3991 4144 4329 4774
Size: 1255 1073 322 999 17 153 185 445
Cuts at: 4774 325
Size: 1498
Fragments arranged by size:
1498 1255 1073 999 445 322 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4193 5905 96
Size: 274 80 33 51 1443 2216 1712 138
Fragments arranged by size:
2216 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2950 2982 5907 5939 2950
Size: 32 2925 32 2958
Fragments arranged by size:
2958 2925 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 2602 3272 1870
Size: 732 670 4545
Fragments arranged by size:
4545 732 670
XmnI GAAnn'nnTTC
Cuts at: 4076 4076
Size: 5947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI
XcmI