Restriction map for
pFN21AB7680
(Circular) (Six-base) MAPSORT of: pFN21AB7680.seq Check: 2604 from: 1 to: 5923
pFN21AB7680 5923 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 3986 5370 5638 1632
Size: 557 1797 1384 268 1917
Fragments arranged by size:
1917 1797 1384 557 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 2208 2597 3666 5238 2208
Size: 389 1069 1572 2893
Fragments arranged by size:
2893 1572 1069 389
ApaI G_GGCC'C
Cuts at: 2464 2741 2761 2464
Size: 277 20 5626
Fragments arranged by size:
5626 277 20
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 3164 3232 3458 3610 5719 1493
Size: 1671 68 226 152 2109 1697
Fragments arranged by size:
2109 1697 1671 226 152 68
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2531 2918 3248 1870
Size: 73 588 387 330 4545
Fragments arranged by size:
4545 588 387 330 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2474 2507 2474
Size: 33 5890
BamHI G'GATC_C
Cuts at: 3214 3253 3620 3214
Size: 39 367 5517
Fragments arranged by size:
5517 367 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2681 2969 3017 3244 4699
Size: 325 217 627 894 288 48 227 1455
Cuts at: 4699 618
Size: 1842
Fragments arranged by size:
1842 1455 894 627 325 288 227 217
48
BanII G_rGCy'C
Cuts at: 1391 2420 2464 2532 2567 2741 2761 2796 3242
Size: 1029 44 68 35 174 20 35 446
Cuts at: 3242 1391
Size: 4072
Fragments arranged by size:
4072 1029 446 174 68 44 35 35
20
BbeI G_GCGC'C
Cuts at: 2973 2973
Size: 5923
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2700 2864 928
Size: 971 801 164 3987
Fragments arranged by size:
3987 971 801 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2899 2933 4114 4148 1903
Size: 34 962 34 1181 34 3678
Fragments arranged by size:
3678 1181 962 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2956 3066 4534
Size: 107 122 71 631 605 1284 110 1468
Cuts at: 4534 136
Size: 1525
Fragments arranged by size:
1525 1468 1284 631 605 122 110 107
71
BglII A'GATC_T
Cuts at: 2331 5918 2331
Size: 3587 2336
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2150 2464 2741 2761 3984 5140 1790
Size: 360 314 277 20 1223 1156 2573
Fragments arranged by size:
2573 1223 1156 360 314 277 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2874 3654 4612
Size: 416 53 83 399 24 1457 780 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1457 958 780 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2404 2982 3083 3126 3167 3209 4583 1239
Size: 1165 578 101 43 41 42 1374 2579
Fragments arranged by size:
2579 1374 1165 578 101 43 42 41
Bpu10I CC'TnA_GC
Cuts at: 2387 2387
Size: 5923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2970 3069 3730
Size: 53 83 186 936 479 958 99 661
Cuts at: 3730 4112 4747 275
Size: 382 635 1451
Fragments arranged by size:
1451 958 936 661 635 479 382 186
99 83 53
BsaWI w'CCGG_w
Cuts at: 2070 2981 4356 4754 5028 5175 5586 2070
Size: 911 1375 398 274 147 411 2407
Fragments arranged by size:
2407 1375 911 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2544 2574 2873 2903 1178
Size: 30 165 30 1141 30 299 30 4198
Fragments arranged by size:
4198 1141 299 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 3039 3056 1186
Size: 1258 595 17 4053
Fragments arranged by size:
4053 1258 595 17
BseYI C'CCAG_C
Cuts at: 2463 2734 2762 3056 5126 5622 2463
Size: 271 28 294 2070 496 2764
Fragments arranged by size:
2764 2070 496 294 271 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 2381 1163
Size: 573 315 330 4705
Fragments arranged by size:
4705 573 330 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2480 3373 4134 5162 1540
Size: 275 186 479 893 761 1028 2301
Fragments arranged by size:
2301 1028 893 761 479 275 186
BsiHKAI G_wGCw'C
Cuts at: 2420 2567 2796 3984 4069 5140 2420
Size: 147 229 1188 85 1071 3203
Fragments arranged by size:
3203 1188 1071 229 147 85
BsmI GAATG_Cn'
Cuts at: 2026 2035 2131 2875 3434 3527 2026
Size: 9 96 744 559 93 4422
Fragments arranged by size:
4422 744 559 96 93 9
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2420 2464 2532 2567 2741 2761
Size: 399 360 270 44 68 35 174 20
Cuts at: 2761 2796 3242 3984 4069 5140 1391
Size: 35 446 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 399 360 270 174
85 68 44 35 35 20
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2270 3278 844
Size: 1426 1008 3489
Fragments arranged by size:
3489 1426 1008
BsrBI CCG'CTC
Cuts at: 1394 1823 2241 2923 3810 1394
Size: 429 418 682 887 3507
Fragments arranged by size:
3507 887 682 429 418
BsrDI GCAATG_nn'
Cuts at: 65 1248 2910 4418 4592 65
Size: 1183 1662 1508 174 1396
Fragments arranged by size:
1662 1508 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2684 4567 4754 1700
Size: 110 204 670 1883 187 2869
Fragments arranged by size:
2869 1883 670 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 2795 2819 3983 4995 2795
Size: 24 1164 1012 3723
Fragments arranged by size:
3723 1164 1012 24
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2452 794
Size: 1658 4265
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 2702 1184
Size: 1518 4405
BstXI CCAn_nnnn'nTGG
Cuts at: 2899 2899
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2331 3214 3253 3620 4005 4022 5474 5560
Size: 359 883 39 367 385 17 1452 86
Cuts at: 5560 5572 5918 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 883 385 367 359 346 86
39 17 12
Bsu36I CC'TnA_GG
Cuts at: 3039 3039
Size: 5923
BtgI C'CryG_G
Cuts at: 513 1065 2614 513
Size: 552 1549 3822
Fragments arranged by size:
3822 1549 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3082 487
Size: 683 56 1856 3328
Fragments arranged by size:
3328 1856 683 56
BtsI GCAGTG_nn'
Cuts at: 2126 2644 3439 4233 4253 2126
Size: 518 795 794 20 3796
Fragments arranged by size:
3796 795 794 518 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2222 4930 809
Size: 507 906 2708 1802
Fragments arranged by size:
2708 1802 906 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2297 2477 3182
Size: 54 1425 50 275 18 467 180 705
Cuts at: 3182 3370 4259 5888 8
Size: 188 889 1629 43
Fragments arranged by size:
1629 1425 889 705 467 275 188 180
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2477 3370 1537
Size: 275 665 893 4090
Fragments arranged by size:
4090 893 665 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3853 1353
Size: 2500 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2372 2563 4510 4884 5030 387
Size: 1064 138 783 191 1947 374 146 1280
Fragments arranged by size:
1947 1280 1064 783 374 191 146 138
EcoICRI GAG'CTC
Cuts at: 2418 2565 2794 2418
Size: 147 229 5547
Fragments arranged by size:
5547 229 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2404 2982 3083 3126 3167 3209
Size: 393 557 215 578 101 43 41 42
Cuts at: 3209 3986 4583 5370 5638 1239
Size: 777 597 787 268 1524
Fragments arranged by size:
1524 787 777 597 578 557 393 268
215 101 43 42 41
EcoO109I rG'GnC_Cy
Cuts at: 2460 2594 2738 2757 2758 2957 2987 2460
Size: 134 144 19 1 199 30 5396
Fragments arranged by size:
5396 199 144 134 30 19 1
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 2909 4429 2909
Size: 1520 4403
HaeII r_GCGC'y
Cuts at: 1678 2973 5070 1678
Size: 1295 2097 2531
Fragments arranged by size:
2531 2097 1295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2364 2396 2549 2581 2873
Size: 32 135 32 792 32 153 32 292
Cuts at: 2873 2905 4564 4596 4638 4670 1373
Size: 32 1659 32 42 32 2626
Fragments arranged by size:
2626 1659 792 292 153 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KasI G'GCGC_C
Cuts at: 2969 2969
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2507 3881 4240 4399 518
Size: 648 1341 1374 359 159 2042
Fragments arranged by size:
2042 1374 1341 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3339 4016 5164 5409 1606
Size: 1733 677 1148 245 2120
Fragments arranged by size:
2120 1733 1148 677 245
NaeI GCC'GGC
Cuts at: 2016 2016
Size: 5923
NarI GG'CG_CC
Cuts at: 2970 2970
Size: 5923
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5371
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NgoMIV G'CCGG_C
Cuts at: 2014 2014
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 2167 2446 3281 4826 1132
Size: 97 938 279 835 1545 2229
Fragments arranged by size:
2229 1545 938 835 279 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2815 3992 4024 5505 5537 2783
Size: 32 1177 32 1481 32 3169
Fragments arranged by size:
3169 1481 1177 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2987 2987
Size: 5923
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 2460 2737 2757 2460
Size: 277 20 5626
Fragments arranged by size:
5626 277 20
PspXI vC'TCGA_Gb
Cuts at: 2918 2918
Size: 5923
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
SacI G_AGCT'C
Cuts at: 2420 2567 2796 2420
Size: 147 229 5547
Fragments arranged by size:
5547 229 147
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 2897 4171 5883 2897
Size: 1274 1712 2937
Fragments arranged by size:
2937 1712 1274
SfcI C'TryA_G
Cuts at: 826 1045 3271 4406 5087 5278 826
Size: 219 2226 1135 681 191 1471
Fragments arranged by size:
2226 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2971 2971
Size: 5923
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 2014 1700
Size: 314 5609
SmaI CCC'GGG
Cuts at: 1872 2533 3250 1872
Size: 661 717 4545
Fragments arranged by size:
4545 717 661
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2918 4027 4928 5190 820
Size: 197 926 975 1109 901 262 1553
Fragments arranged by size:
1553 1109 975 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 2446 3281 1132
Size: 1314 835 3774
Fragments arranged by size:
3774 1314 835
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2187 3210 3362 513
Size: 552 1122 1023 152 3074
Fragments arranged by size:
3074 1122 1023 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2112 2385 2663 2985 3950 3967 4120
Size: 1255 532 273 278 322 965 17 153
Cuts at: 4120 4305 4750 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 965 532 445 322 278 273
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 3084 4169
Size: 274 80 33 51 1443 918 189 1085
Cuts at: 4169 5881 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1085 918 274 189 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2544 2576 3199 3231 5883 5915 2544
Size: 32 623 32 2652 32 2552
Fragments arranged by size:
2652 2552 623 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 2918 1943
Size: 975 4948
XmaI C'CCGG_G
Cuts at: 1870 2531 3248 1870
Size: 661 717 4545
Fragments arranged by size:
4545 717 661
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI