Restriction map for
pFN21AB7678
(Circular) (Six-base) MAPSORT of: pFN21AB7678.seq Check: 139 from: 1 to: 5923
pFN21AB7678 5923 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 2184 4050 4423 1563
Size: 621 1866 373 3063
Fragments arranged by size:
3063 1866 621 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2562 3986 5370 5638 1632
Size: 710 220 1424 1384 268 1917
Fragments arranged by size:
1917 1424 1384 710 268 220
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 2652 3142 4822 5799 1203
Size: 1449 490 1680 977 1327
Fragments arranged by size:
1680 1449 1327 977 490
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 3666 5238 3666
Size: 1572 4351
ApaI G_GGCC'C
Cuts at: 3187 3187
Size: 5923
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2533 3232 3458 3610 5719 1493
Size: 1040 699 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 699 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2315 2864 2972 2999 3026 3053 3080
Size: 73 372 549 108 27 27 27 27
Cuts at: 3080 3107 3248 1870
Size: 27 141 4545
Fragments arranged by size:
4545 549 372 141 108 73 27 27
27 27 27
BamHI G'GATC_C
Cuts at: 2692 3253 3620 2692
Size: 561 367 4995
Fragments arranged by size:
4995 561 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2716 2824 2851 2878 2905
Size: 325 217 627 929 108 27 27 27
Cuts at: 2905 2932 2959 3040 3067 3094 3121 3244 4699
Size: 27 27 81 27 27 27 123 1455
Cuts at: 4699 618
Size: 1842
Fragments arranged by size:
1842 1455 929 627 325 217 123 108
81 27 27 27 27 27 27 27
27
BanII G_rGCy'C
Cuts at: 1391 3187 3242 1391
Size: 1796 55 4072
Fragments arranged by size:
4072 1796 55
BbeI G_GCGC'C
Cuts at: 2828 2855 2882 2909 2936 2963 3044 3071 3098
Size: 27 27 27 27 27 81 27 27
Cuts at: 3098 3125 2828
Size: 27 5626
Fragments arranged by size:
5626 81 27 27 27 27 27 27
27 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2550 928
Size: 971 651 4301
Fragments arranged by size:
4301 971 651
BbvCI CC'TCA_GC
Cuts at: 2394 2394
Size: 5923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BfrBI ATG'CAT
Cuts at: 3209 3209
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2767 2794 2866
Size: 107 122 71 631 605 1095 27 72
Cuts at: 2866 2893 2920 2947 2974 3001 3028 3055 3082
Size: 27 27 27 27 27 27 27 27
Cuts at: 3082 3109 4534 136
Size: 27 1425 1525
Fragments arranged by size:
1525 1425 1095 631 605 122 107 72
71 27 27 27 27 27 27 27
27 27 27
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 3187 3984 5140 1790
Size: 1397 797 1156 2573
Fragments arranged by size:
2573 1397 1156 797
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3654 4612 442
Size: 416 53 83 399 24 2237 958 1753
Fragments arranged by size:
2237 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4583 1239
Size: 3344 2579
Bpu10I CC'TnA_GC
Cuts at: 2394 2394
Size: 5923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2825 2852 2879 2906
Size: 53 83 186 936 1292 27 27 27
Cuts at: 2906 2933 2960 3041 3068 3095 3122 3730 4112
Size: 27 27 81 27 27 27 608 382
Cuts at: 4112 4747 275
Size: 635 1451
Fragments arranged by size:
1451 1292 936 635 608 382 186 83
81 53 27 27 27 27 27 27
27 27
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5698
Fragments arranged by size:
5698 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2139 2150 2279 2315 2351 1186
Size: 953 11 129 36 36 4758
Fragments arranged by size:
4758 953 129 36 36 11
BseYI C'CCAG_C
Cuts at: 5126 5622 5126
Size: 496 5427
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2573 1163
Size: 573 837 4513
Fragments arranged by size:
4513 837 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2355 3373 4134 5162 1540
Size: 275 186 354 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 354 275 186
BsiHKAI G_wGCw'C
Cuts at: 3984 4069 5140 3984
Size: 85 1071 4767
Fragments arranged by size:
4767 1071 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3187 3242 3984 4069 5140 1391
Size: 399 1397 55 742 85 1071 2174
Fragments arranged by size:
2174 1397 1071 742 399 85 55
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2545 3278 844
Size: 1701 733 3489
Fragments arranged by size:
3489 1701 733
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2371 4567 4754 1700
Size: 110 561 2196 187 2869
Fragments arranged by size:
2869 2196 561 187 110
BsrGI T'GTAC_A
Cuts at: 96 3169 96
Size: 3073 2850
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 2243 3983 4995 2243
Size: 1740 1012 3171
Fragments arranged by size:
3171 1740 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2692 3253 3620 4005 4022 5474 5560 5572
Size: 720 561 367 385 17 1452 86 12
Cuts at: 5572 5918 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 720 561 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 2775 2820 2901 2928 2955
Size: 552 939 351 420 45 81 27 27
Cuts at: 2955 2982 3009 3036 3063 3090 3117 513
Size: 27 27 27 27 27 27 3319
Fragments arranged by size:
3319 939 552 420 351 81 45 27
27 27 27 27 27 27 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2619 3439 4233 4253 2619
Size: 820 794 20 4289
Fragments arranged by size:
4289 820 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2239 2894 2921
Size: 54 1425 50 275 18 409 655 27
Cuts at: 2921 3370 4259 5888 8
Size: 449 889 1629 43
Fragments arranged by size:
1629 1425 889 655 449 409 275 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2019 2569 3853 1353
Size: 666 550 1284 3423
Fragments arranged by size:
3423 1284 666 550
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2390 4510 4884 5030 387
Size: 1064 138 801 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 801 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2562 3986 4583 5370 5638 1239
Size: 393 710 220 1424 597 787 268 1524
Fragments arranged by size:
1524 1424 787 710 597 393 268 220
EcoO109I rG'GnC_Cy
Cuts at: 2667 3184 2667
Size: 517 5406
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2828 2855 2882 2909 2936 2963 3044 3071
Size: 1150 27 27 27 27 27 81 27
Cuts at: 3071 3098 3125 5070 1678
Size: 27 27 1945 2531
Fragments arranged by size:
2531 1945 1150 81 27 27 27 27
27 27 27 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373
Size: 32 135 32 2992 32 42 32 2626
Fragments arranged by size:
2992 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KasI G'GCGC_C
Cuts at: 2824 2851 2878 2905 2932 2959 3040 3067 3094
Size: 27 27 27 27 27 81 27 27
Cuts at: 3094 3121 2824
Size: 27 5626
Fragments arranged by size:
5626 81 27 27 27 27 27 27
27 27
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3881 4240 4399 518
Size: 648 2715 359 159 2042
Fragments arranged by size:
2715 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2393 2455 3339 4016 5164 5409 1606
Size: 787 62 884 677 1148 245 2120
Fragments arranged by size:
2120 1148 884 787 677 245 62
NaeI GCC'GGC
Cuts at: 2373 2373
Size: 5923
NarI GG'CG_CC
Cuts at: 2825 2852 2879 2906 2933 2960 3041 3068 3095
Size: 27 27 27 27 27 81 27 27
Cuts at: 3095 3122 2825
Size: 27 5626
Fragments arranged by size:
5626 81 27 27 27 27 27 27
27 27
NcoI C'CATG_G
Cuts at: 513 1065 2004 2775 2820 2901 2928 2955 2982
Size: 552 939 771 45 81 27 27 27
Cuts at: 2982 3009 3036 3063 3090 3117 513
Size: 27 27 27 27 27 3319
Fragments arranged by size:
3319 939 771 552 81 45 27 27
27 27 27 27 27 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NgoMIV G'CCGG_C
Cuts at: 2371 2371
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NsiI A_TGCA'T
Cuts at: 3211 3211
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 2656 3146 3213 3281 4826 1132
Size: 97 1427 490 67 68 1545 2229
Fragments arranged by size:
2229 1545 1427 490 97 68 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 2652 3142 4822 2652
Size: 490 1680 3753
Fragments arranged by size:
3753 1680 490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 1108
Size: 1539 4384
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 3183 3183
Size: 5923
PspXI vC'TCGA_Gb
Cuts at: 2315 2315
Size: 5923
PstI C_TGCA'G
Cuts at: 830 2460 3275 830
Size: 1630 815 3478
Fragments arranged by size:
3478 1630 815
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2455 2455
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SapI GCTCTTCn'nnn_
Cuts at: 2569 2569
Size: 5923
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 2456 3271 4406 5087 5278 826
Size: 219 1411 815 1135 681 191 1471
Fragments arranged by size:
1471 1411 1135 815 681 219 191
SfoI GGC'GCC
Cuts at: 2826 2853 2880 2907 2934 2961 3042 3069 3096
Size: 27 27 27 27 27 81 27 27
Cuts at: 3096 3123 2826
Size: 27 5626
Fragments arranged by size:
5626 81 27 27 27 27 27 27
27 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 2866 2974 3001 3028 3055 3082 3109 3250
Size: 994 108 27 27 27 27 27 141
Cuts at: 3250 1872
Size: 4545
Fragments arranged by size:
4545 994 141 108 27 27 27 27
27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 4027 4928 5190 820
Size: 197 926 372 1712 901 262 1553
Fragments arranged by size:
1712 1553 926 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2775 2820 2901 2928 2955 2982
Size: 552 939 771 45 81 27 27 27
Cuts at: 2982 3009 3036 3063 3090 3117 3362 513
Size: 27 27 27 27 27 245 3074
Fragments arranged by size:
3074 939 771 552 245 81 45 27
27 27 27 27 27 27 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2042 2651 2779 2788 2806 2815 2833
Size: 1255 462 609 128 9 18 9 18
Cuts at: 2833 3130 3148 3950 3967 4120 4305 4750 325
Size: 297 18 802 17 153 185 445 1498
Fragments arranged by size:
1498 1255 802 609 462 445 297 185
153 128 18 18 18 17 9 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3169 4169 5881
Size: 274 80 33 51 1443 1192 1000 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1712 1443 1192 1000 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2763 2795 5883 5915 2763
Size: 32 3088 32 2771
Fragments arranged by size:
3088 2771 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2036 2773 2036
Size: 737 5186
XhoI C'TCGA_G
Cuts at: 1943 2315 1943
Size: 372 5551
XmaI C'CCGG_G
Cuts at: 1870 2864 2972 2999 3026 3053 3080 3107 3248
Size: 994 108 27 27 27 27 27 141
Cuts at: 3248 1870
Size: 4545
Fragments arranged by size:
4545 994 141 108 27 27 27 27
27
XmnI GAAnn'nnTTC
Cuts at: 2474 4052 2474
Size: 1578 4345
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI PacI PfoI PmlI PpuMI
PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI