Restriction map for
pFN21AB7677
(Circular) (Six-base) MAPSORT of: pFN21AB7677.seq Check: 7477 from: 1 to: 5728
pFN21AB7677 5728 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2487 2487
Size: 5728
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2319 3538 278
Size: 53 83 186 936 783 1219 2468
Fragments arranged by size:
2468 1219 936 783 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3071 1937
Size: 1134 4594
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2377 2420 3791 5175 5443 1632
Size: 745 43 1371 1384 268 1917
Fragments arranged by size:
1917 1384 1371 745 268 43
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 2691 4627 5604 1203
Size: 1488 1936 977 1327
Fragments arranged by size:
1936 1488 1327 977
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 2072 1166
Size: 906 4822
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2090 1184
Size: 906 4822
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 2225
Size: 32 5696
AlwNI CAG_nnn'CTG
Cuts at: 2796 3471 5043 2796
Size: 675 1572 3481
Fragments arranged by size:
3481 1572 675
ApaI G_GGCC'C
Cuts at: 2376 2376
Size: 5728
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 3037 3263 3415 5524 1493
Size: 1544 226 152 2109 1697
Fragments arranged by size:
2109 1697 1544 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 3053 1870
Size: 73 1110 4545
Fragments arranged by size:
4545 1110 73
AvrII C'CTAG_G
Cuts at: 2062 2062
Size: 5728
BamHI G'GATC_C
Cuts at: 2773 3058 3425 2773
Size: 285 367 5076
Fragments arranged by size:
5076 367 285
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2292 3049 4504 618
Size: 325 217 627 279 226 757 1455 1842
Fragments arranged by size:
1842 1455 757 627 325 279 226 217
BanII G_rGCy'C
Cuts at: 1391 2362 2376 2598 2727 2787 3047 1391
Size: 971 14 222 129 60 260 4072
Fragments arranged by size:
4072 971 260 222 129 60 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2146 2408 2748 928
Size: 971 247 262 340 3908
Fragments arranged by size:
3908 971 340 262 247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3919 3953 1903
Size: 34 50 34 1898 34 3678
Fragments arranged by size:
3678 1898 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5728
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4339 136
Size: 107 122 71 631 605 2667 1525
Fragments arranged by size:
2667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2757 5723 2757
Size: 2966 2762
BlpI GC'TnA_GC
Cuts at: 3145 3145
Size: 5728
Bme1580I G_kGCm'C
Cuts at: 1790 2376 2851 3789 4945 1790
Size: 586 475 938 1156 2573
Fragments arranged by size:
2573 1156 938 586 475
BmgBI CAC'GTC
Cuts at: 1318 2179 1318
Size: 861 4867
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3459 4417 442
Size: 416 53 83 399 24 2042 958 1753
Fragments arranged by size:
2042 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2406 2981 2990 4388 1239
Size: 1079 88 575 9 1398 2579
Fragments arranged by size:
2579 1398 1079 575 88 9
Bpu10I CC'TnA_GC
Cuts at: 2308 2308
Size: 5728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3853 4718 5016 3853
Size: 865 298 4565
Fragments arranged by size:
4565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4391 882
Size: 617 2892 2219
Fragments arranged by size:
2892 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2074 2692 3519 493
Size: 711 870 618 827 2702
Fragments arranged by size:
2702 870 827 711 618
BsaBI GATnn'nnATC
Cuts at: 5722 5722
Size: 5728
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2405 2751 3535
Size: 53 83 186 936 783 89 346 784
Cuts at: 3535 3917 4552 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 784 783 635 382 346 186
89 83 53
BsaWI w'CCGG_w
Cuts at: 3025 4161 4559 4833 4980 5391 3025
Size: 1136 398 274 147 411 3362
Fragments arranged by size:
3362 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 1178
Size: 30 165 30 822 30 4651
Fragments arranged by size:
4651 822 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2579 1186
Size: 1393 4335
BseYI C'CCAG_C
Cuts at: 2107 2196 2303 2349 2412 4931 5427 2107
Size: 89 107 46 63 2519 496 2408
Fragments arranged by size:
2519 2408 496 107 89 63 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2168 2610 1163
Size: 573 432 442 4281
Fragments arranged by size:
4281 573 442 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3939 4967 1540
Size: 275 186 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 2598 3789 3874 4945 2238
Size: 360 1191 85 1071 3021
Fragments arranged by size:
3021 1191 1071 360 85
BsmI GAATG_Cn'
Cuts at: 3239 3332 3239
Size: 93 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 2362 2376 2598 2727 2787 2851
Size: 399 448 124 14 222 129 60 64
Cuts at: 2851 3047 3789 3874 4945 1391
Size: 196 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 448 399 222 196 129
124 85 64 60 14
BspEI T'CCGG_A
Cuts at: 5391 5391
Size: 5728
BspHI T'CATG_A
Cuts at: 3617 5347 3617
Size: 1730 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2487 3083 844
Size: 1268 375 596 3489
Fragments arranged by size:
3489 1268 596 375
BsrBI CCG'CTC
Cuts at: 1394 1823 3615 1394
Size: 429 1792 3507
Fragments arranged by size:
3507 1792 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4223 4397 65
Size: 1183 2975 174 1396
Fragments arranged by size:
2975 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4372 4559 1700
Size: 110 2562 187 2869
Fragments arranged by size:
2869 2562 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 2844 3788 4800 2844
Size: 944 1012 3772
Fragments arranged by size:
3772 1012 944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2191 794
Size: 1397 4331
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstYI r'GATC_y
Cuts at: 1972 2121 2388 2757 2773 3058 3425 3810 3827
Size: 149 267 369 16 285 367 385 17
Cuts at: 3827 5279 5365 5377 5723 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 369 367 346 285 267
149 86 17 16 12
BtgI C'CryG_G
Cuts at: 513 1065 2229 513
Size: 552 1164 4012
Fragments arranged by size:
4012 1164 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2648 487
Size: 683 56 1422 3567
Fragments arranged by size:
3567 1422 683 56
BtsI GCAGTG_nn'
Cuts at: 3244 4038 4058 3244
Size: 794 20 4914
Fragments arranged by size:
4914 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2518 3175 4064
Size: 54 1425 50 275 18 688 657 889
Cuts at: 4064 5693 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 688 657 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2430 3658 1353
Size: 1077 1228 3423
Fragments arranged by size:
3423 1228 1077
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2596 2596
Size: 5728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 2377 2406 2420 2981 2990 3791
Size: 393 686 59 29 14 561 9 801
Cuts at: 3791 4388 5175 5443 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 801 787 686 597 561 393 268
59 29 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2061 2276 2061
Size: 215 5513
EcoO109I rG'GnC_Cy
Cuts at: 2373 2607 2644 2373
Size: 234 37 5457
Fragments arranged by size:
5457 234 37
EcoRI G'AATT_C
Cuts at: 3037 3415 3037
Size: 378 5350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 5728
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 4875 1678
Size: 3197 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2133 2165 2247 2279 2825
Size: 32 135 32 561 32 82 32 546
Cuts at: 2825 2857 4369 4401 4443 4475 1373
Size: 32 1512 32 42 32 2626
Fragments arranged by size:
2626 1512 561 546 135 82 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3072 3318 669
Size: 988 281 1134 246 3079
Fragments arranged by size:
3079 1134 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2867 748
Size: 2119 3609
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 3327 3327
Size: 5728
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4842 5026 1305
Size: 3537 184 2007
Fragments arranged by size:
3537 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3906
Fragments arranged by size:
3906 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2526 3686 4045 4204 518
Size: 648 906 454 1160 359 159 2042
Fragments arranged by size:
2042 1160 906 648 454 359 159
MspA1I CmG'CkG
Cuts at: 1606 2231 2353 2701 2968 3144 3821 4969 5214
Size: 625 122 348 267 176 677 1148 245
Cuts at: 5214 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 625 348 267 245 176
122
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5176
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NspI r_CATG'y
Cuts at: 1132 1229 3086 4631 1132
Size: 97 1857 1545 2229
Fragments arranged by size:
2229 1857 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2498 2611 1348
Size: 1150 113 4465
Fragments arranged by size:
4465 1150 113
PciI A'CATG_T
Cuts at: 4627 4627
Size: 5728
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2796 1108
Size: 1688 4040
PfoI T'CCnGG_A
Cuts at: 2926 2940 2926
Size: 14 5714
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PmlI CAC'GTG
Cuts at: 2074 2074
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2225 2257 3797 3829 5310 5342 2225
Size: 32 1540 32 1481 32 2611
Fragments arranged by size:
2611 1540 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2607 2607
Size: 5728
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 2865 3298 2865
Size: 433 5295
PspOMI G'GGCC_C
Cuts at: 2372 2372
Size: 5728
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 5696
PstI C_TGCA'G
Cuts at: 830 2487 2606 3080 830
Size: 1657 119 474 3478
Fragments arranged by size:
3478 1657 474 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
PvuII CAG'CTG
Cuts at: 2701 2701
Size: 5728
SacI G_AGCT'C
Cuts at: 2598 2598
Size: 5728
SacII CC_GC'GG
Cuts at: 2232 2232
Size: 5728
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 2213 3976 5688 2213
Size: 1763 1712 2253
Fragments arranged by size:
2253 1763 1712
SfcI C'TryA_G
Cuts at: 826 1045 2483 2602 3076 4211 4892 5083 826
Size: 219 1438 119 474 1135 681 191 1471
Fragments arranged by size:
1471 1438 1135 681 474 219 191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 3055 1872
Size: 1183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3832 4733 4995 820
Size: 197 926 1889 901 262 1553
Fragments arranged by size:
1889 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 3086 1132
Size: 1954 3774
SspI AAT'ATT
Cuts at: 5 52 3652 5
Size: 47 3600 2081
Fragments arranged by size:
3600 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 2062 2277 3167 513
Size: 552 997 215 890 3074
Fragments arranged by size:
3074 997 890 552 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 2099 2426 3755 3772 3925 4110
Size: 1255 504 15 327 1329 17 153 185
Cuts at: 4110 4555 325
Size: 445 1498
Fragments arranged by size:
1498 1329 1255 504 445 327 185 153
17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 2337 3974
Size: 274 80 33 51 1443 234 126 1637
Cuts at: 3974 5686 96
Size: 1712 138
Fragments arranged by size:
1712 1637 1443 274 234 138 126 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5688 5720 5688
Size: 32 5696
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5728
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5728
XmaI C'CCGG_G
Cuts at: 1870 3053 1870
Size: 1183 4545
XmnI GAAnn'nnTTC
Cuts at: 3857 3857
Size: 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2317 3536 276
Size: 53 83 186 936 783 1219 2468
Fragments arranged by size:
2468 1219 936 783 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI