Restriction map for
pFN21AB7676
(Circular) (Six-base) MAPSORT of: pFN21AB7676.seq Check: 584 from: 1 to: 5923
pFN21AB7676 5923 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3143 3733 278
Size: 53 83 186 936 1607 590 2468
Fragments arranged by size:
2468 1607 936 590 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2493 3011 3266 1937
Size: 556 518 255 4594
Fragments arranged by size:
4594 556 518 255
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3986 5370 5638 1632
Size: 2354 1384 268 1917
Fragments arranged by size:
2354 1917 1384 268
AfeI AGC'GCT
Cuts at: 2345 2345
Size: 5923
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 2438 4822 5799 1203
Size: 1235 2384 977 1327
Fragments arranged by size:
2384 1327 1235 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 2999 1166
Size: 1833 4090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 3666 5238 3666
Size: 1572 4351
ApaI G_GGCC'C
Cuts at: 2030 2702 3050 2030
Size: 672 348 4903
Fragments arranged by size:
4903 672 348
ApaLI G'TGCA_C
Cuts at: 2036 2431 3980 5136 2036
Size: 395 1549 1156 2823
Fragments arranged by size:
2823 1549 1156 395
ApoI r'AATT_y
Cuts at: 1493 3232 3458 3610 5719 1493
Size: 1739 226 152 2109 1697
Fragments arranged by size:
2109 1739 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2397 2695 2785 2955 3248 1870
Size: 73 87 367 298 90 170 293 4545
Fragments arranged by size:
4545 367 298 293 170 90 87 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3244 4699 618
Size: 325 217 627 1457 1455 1842
Fragments arranged by size:
1842 1457 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2030 2277 2696 2702 3050 3063 3242 1391
Size: 639 247 419 6 348 13 179 4072
Fragments arranged by size:
4072 639 419 348 247 179 13 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 2627 2804 928
Size: 971 423 305 177 4047
Fragments arranged by size:
4047 971 423 305 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4114 4148 1903
Size: 34 50 34 2093 34 3678
Fragments arranged by size:
3678 2093 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2364 2561 2588
Size: 107 122 71 631 605 692 197 27
Cuts at: 2588 4534 136
Size: 1946 1525
Fragments arranged by size:
1946 1525 692 631 605 197 122 107
71 27
BglII A'GATC_T
Cuts at: 2181 5918 2181
Size: 3737 2186
BlpI GC'TnA_GC
Cuts at: 2735 3077 3340 2735
Size: 342 263 5318
Fragments arranged by size:
5318 342 263
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2040 2435 2660 2702 3050 3984 5140
Size: 240 10 395 225 42 348 934 1156
Cuts at: 5140 1790
Size: 2573
Fragments arranged by size:
2573 1156 934 395 348 240 225 42
10
BmgBI CAC'GTC
Cuts at: 1318 2560 2817 1318
Size: 1242 257 4424
Fragments arranged by size:
4424 1242 257
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3654 4612 442
Size: 416 53 83 399 24 2237 958 1753
Fragments arranged by size:
2237 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2097 2097
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2918 3070 3191 4583 1239
Size: 1046 633 152 121 1392 2579
Fragments arranged by size:
2579 1392 1046 633 152 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2222 4048 4913 5211 2222
Size: 1826 865 298 2934
Fragments arranged by size:
2934 1826 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2430 2439 3714 493
Size: 711 1226 9 1275 2702
Fragments arranged by size:
2702 1275 1226 711 9
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2880 3038 3140 3730
Size: 53 83 186 936 1347 158 102 590
Cuts at: 3730 4112 4747 275
Size: 382 635 1451
Fragments arranged by size:
1451 1347 936 635 590 382 186 158
102 83 53
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5698
Fragments arranged by size:
5698 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5923
BseYI C'CCAG_C
Cuts at: 2605 5126 5622 2605
Size: 2521 496 2906
Fragments arranged by size:
2906 2521 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 2684 1163
Size: 573 624 324 4402
Fragments arranged by size:
4402 624 573 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2107 2574 2678 3373 4134 5162
Size: 275 186 106 467 104 695 761 1028
Cuts at: 5162 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 695 467 275 186 106
104
BsiHKAI G_wGCw'C
Cuts at: 2040 2277 2435 2763 3063 3984 4069 5140 2040
Size: 237 158 328 300 921 85 1071 2823
Fragments arranged by size:
2823 1071 921 328 300 237 158 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
BsmBI CGTCTCn'nnnn_
Cuts at: 2114 2114
Size: 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2040 2277 2435 2660 2696 2702
Size: 399 240 10 237 158 225 36 6
Cuts at: 2702 2763 3050 3063 3242 3984 4069 5140 1391
Size: 61 287 13 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 287 240 237 225
179 158 85 61 36 13 10 6
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 3278 844
Size: 1364 1070 3489
Fragments arranged by size:
3489 1364 1070
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2165 4567 4754 1700
Size: 110 355 2402 187 2869
Fragments arranged by size:
2869 2402 355 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 2392 1925
Size: 467 5456
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2307 794
Size: 1513 4410
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 2629 2629
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2181 2710 3253 3620 4005 4022 5474 5560
Size: 209 529 543 367 385 17 1452 86
Cuts at: 5560 5572 5918 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 543 529 385 367 346 209
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2107 2457 2583 513
Size: 552 1042 350 126 3853
Fragments arranged by size:
3853 1042 552 350 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2149 2225 3439 4233 4253 2149
Size: 76 1214 794 20 3819
Fragments arranged by size:
3819 1214 794 76 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DraIII CAC_nnn'GTG
Cuts at: 2439 2439
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2131 2489 2848 4930 809
Size: 507 815 358 359 2082 1802
Fragments arranged by size:
2082 1802 815 507 359 358
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2589 2675
Size: 54 1425 50 275 18 741 18 86
Cuts at: 2675 3370 4259 5888 8
Size: 695 889 1629 43
Fragments arranged by size:
1629 1425 889 741 695 275 86 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2571 2675 3370 1537
Size: 275 759 104 695 4090
Fragments arranged by size:
4090 759 695 275 104
EarI CTCTTCn'nnn_
Cuts at: 1353 3853 1353
Size: 2500 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2086 4510 4884 5030 387
Size: 1064 138 497 2424 374 146 1280
Fragments arranged by size:
2424 1280 1064 497 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 3061 2275
Size: 786 5137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2918 3070 3191 3986 4583 5370
Size: 393 653 633 152 121 795 597 787
Cuts at: 5370 5638 1239
Size: 268 1524
Fragments arranged by size:
1524 795 787 653 633 597 393 268
152 121
EcoO109I rG'GnC_Cy
Cuts at: 2027 2699 2929 2027
Size: 672 230 5021
Fragments arranged by size:
5021 672 230
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 5923
FspI TGC'GCA
Cuts at: 2813 4429 2813
Size: 1616 4307
FspAI rTGC'GCAy
Cuts at: 2813 2813
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2332 2347 2519 3026 5070 1678
Size: 654 15 172 507 2044 2531
Fragments arranged by size:
2531 2044 654 507 172 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2380 2412 4564 4596 4638
Size: 32 135 32 808 32 2152 32 42
Cuts at: 4638 4670 1373
Size: 32 2626
Fragments arranged by size:
2626 2152 808 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3267 3513 669
Size: 988 281 1074 255 246 3079
Fragments arranged by size:
3079 1074 988 281 255 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 3090 5037 5221 1305
Size: 945 840 1947 184 2007
Fragments arranged by size:
2007 1947 945 840 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2999 3881 4240 4399 518
Size: 648 1833 882 359 159 2042
Fragments arranged by size:
2042 1833 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2109 2355 2567 2576 2585 3339 4016 5164
Size: 503 246 212 9 9 754 677 1148
Cuts at: 5164 5409 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 754 677 503 246 245 212
9 9
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 5923
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5371
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 2571 3370 2571
Size: 799 5124
NspI r_CATG'y
Cuts at: 1132 1229 2230 2812 3281 4826 1132
Size: 97 1001 582 469 1545 2229
Fragments arranged by size:
2229 1545 1001 582 469 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PmlI CAC'GTG
Cuts at: 2430 2439 2430
Size: 9 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2929 2929
Size: 5923
PshAI GACnn'nnGTC
Cuts at: 1613 3143 1613
Size: 1530 4393
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 2026 2698 3046 2026
Size: 672 348 4903
Fragments arranged by size:
4903 672 348
PstI C_TGCA'G
Cuts at: 830 2526 2581 3030 3275 830
Size: 1696 55 449 245 3478
Fragments arranged by size:
3478 1696 449 245 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
RsrII CG'GwC_CG
Cuts at: 2188 2188
Size: 5923
SacI G_AGCT'C
Cuts at: 2277 3063 2277
Size: 786 5137
SacII CC_GC'GG
Cuts at: 2110 2586 2110
Size: 476 5447
SalI G'TCGA_C
Cuts at: 1936 3010 3265 1936
Size: 1074 255 4594
Fragments arranged by size:
4594 1074 255
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 2323 2522 2577 3026 3271 4406 5087
Size: 219 1278 199 55 449 245 1135 681
Cuts at: 5087 5278 826
Size: 191 1471
Fragments arranged by size:
1471 1278 1135 681 449 245 219 199
191 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 2697 3250 1872
Size: 825 553 4545
Fragments arranged by size:
4545 825 553
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2237 4027 4928 5190 820
Size: 197 926 294 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 294 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 2812 3281 1132
Size: 1680 469 3774
Fragments arranged by size:
3774 1680 469
SrfI GCCC'GGGC
Cuts at: 2697 2697
Size: 5923
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2883 3362 513
Size: 552 1818 479 3074
Fragments arranged by size:
3074 1818 552 479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3950 3967 4120 4305 4750 325
Size: 1255 2370 17 153 185 445 1498
Fragments arranged by size:
2370 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4169 5881 96
Size: 274 80 33 51 1443 2192 1712 138
Fragments arranged by size:
2192 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 5883 5915 2204
Size: 32 3647 32 2212
Fragments arranged by size:
3647 2212 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 2695 3248 1870
Size: 825 553 4545
Fragments arranged by size:
4545 825 553
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3141 3731 276
Size: 53 83 186 936 1607 590 2468
Fragments arranged by size:
2468 1607 936 590 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI RsrII SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI KasI
NarI NruI NsiI PacI PfoI PspXI PsrI PvuII
SanDI SapI SexAI SfiI SfoI SwaI XcmI