Restriction map for
pFN21AB7666
(Circular) (Six-base) MAPSORT of: pFN21AB7666.seq Check: 9463 from: 1 to: 5818
pFN21AB7666 5818 bp
With 182 enzymes: SgfI *
January 9, 2015 16:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3161 1937
Size: 1224 4594
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3881 5265 5533 1632
Size: 2249 1384 268 1917
Fragments arranged by size:
2249 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 2379 4717 5694 1203
Size: 1176 2338 977 1327
Fragments arranged by size:
2338 1327 1176 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 2164 4547 2164
Size: 2383 3435
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5818
AlwNI CAG_nnn'CTG
Cuts at: 2576 3561 5133 2576
Size: 985 1572 3261
Fragments arranged by size:
3261 1572 985
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 3127 3353 3505 5614 1493
Size: 1634 226 152 2109 1697
Fragments arranged by size:
2109 1697 1634 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2329 2410 3143 1870
Size: 73 386 81 733 4545
Fragments arranged by size:
4545 733 386 81 73
BamHI G'GATC_C
Cuts at: 3148 3515 3148
Size: 367 5451
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3139 4594 618
Size: 325 217 627 1352 1455 1842
Fragments arranged by size:
1842 1455 1352 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2250 3137 1391
Size: 859 887 4072
Fragments arranged by size:
4072 887 859
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 928
Size: 971 261 4586
Fragments arranged by size:
4586 971 261
BbvCI CC'TCA_GC
Cuts at: 2772 2772
Size: 5818
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4009 4043 1903
Size: 34 50 34 1988 34 3678
Fragments arranged by size:
3678 1988 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4429 136
Size: 107 122 71 631 605 2757 1525
Fragments arranged by size:
2757 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2112 5813 2112
Size: 3701 2117
BlpI GC'TnA_GC
Cuts at: 3235 3235
Size: 5818
Bme1580I G_kGCm'C
Cuts at: 1790 2433 3879 5035 1790
Size: 643 1446 1156 2573
Fragments arranged by size:
2573 1446 1156 643
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5818
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3549 4507 442
Size: 416 53 83 399 24 2132 958 1753
Fragments arranged by size:
2132 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2487 2553 4478 1239
Size: 1248 66 1925 2579
Fragments arranged by size:
2579 1925 1248 66
Bpu10I CC'TnA_GC
Cuts at: 2772 2772
Size: 5818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3943 4808 5106 3943
Size: 865 298 4655
Fragments arranged by size:
4655 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4481 882
Size: 617 2982 2219
Fragments arranged by size:
2982 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2764 3609 493
Size: 711 1560 845 2702
Fragments arranged by size:
2702 1560 845 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3625 4007 4642 275
Size: 53 83 186 936 2092 382 635 1451
Fragments arranged by size:
2092 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2018 4251 4649 4923 5070 5481 2018
Size: 2233 398 274 147 411 2355
Fragments arranged by size:
2355 2233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5593
Fragments arranged by size:
5593 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2037 1186
Size: 851 4967
BseYI C'CCAG_C
Cuts at: 5021 5517 5021
Size: 496 5322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 2919 1163
Size: 573 723 460 4062
Fragments arranged by size:
4062 723 573 460
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3268 4029 5057 1540
Size: 275 186 1267 761 1028 2301
Fragments arranged by size:
2301 1267 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2496 3879 3964 5035 2496
Size: 1383 85 1071 3279
Fragments arranged by size:
3279 1383 1071 85
BsmI GAATG_Cn'
Cuts at: 2836 3329 3422 2836
Size: 493 93 5232
Fragments arranged by size:
5232 493 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2433 2496 3137 3879 3964 5035
Size: 399 460 183 63 641 742 85 1071
Cuts at: 5035 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 641 460 399 183 85
63
BspEI T'CCGG_A
Cuts at: 2018 5481 2018
Size: 3463 2355
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3173 844
Size: 2329 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3705 1394
Size: 429 1882 3507
Fragments arranged by size:
3507 1882 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4313 4487 65
Size: 1183 3065 174 1396
Fragments arranged by size:
3065 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4462 4649 1700
Size: 110 2652 187 2869
Fragments arranged by size:
2869 2652 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5818
BssSI C'ACGA_G
Cuts at: 2213 3878 4890 2213
Size: 1665 1012 3141
Fragments arranged by size:
3141 1665 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5818
BstBI TT'CG_AA
Cuts at: 4622 4622
Size: 5818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstXI CCAn_nnnn'nTGG
Cuts at: 2687 2918 2687
Size: 231 5587
BstYI r'GATC_y
Cuts at: 1972 2112 2390 3148 3515 3900 3917 5369 5455
Size: 140 278 758 367 385 17 1452 86
Cuts at: 5455 5467 5813 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 758 385 367 346 278 140
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2718 2718
Size: 5818
BtgI C'CryG_G
Cuts at: 513 1065 2061 2433 513
Size: 552 996 372 3898
Fragments arranged by size:
3898 996 552 372
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5079
Fragments arranged by size:
5079 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DraIII CAC_nnn'GTG
Cuts at: 2218 2218
Size: 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2220 3265
Size: 54 1425 50 275 18 369 21 1045
Cuts at: 3265 4154 5783 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1045 889 369 275 54 50
43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3265 1537
Size: 275 1453 4090
Fragments arranged by size:
4090 1453 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2943 3044 3050 3748 1353
Size: 780 810 101 6 698 3423
Fragments arranged by size:
3423 810 780 698 101 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4405 4779 4925 387
Size: 1064 138 2816 374 146 1280
Fragments arranged by size:
2816 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2487 2553 3881 4478 5265 5533 1239
Size: 393 855 66 1328 597 787 268 1524
Fragments arranged by size:
1524 1328 855 787 597 393 268 66
EcoO109I rG'GnC_Cy
Cuts at: 2044 2318 2044
Size: 274 5544
EcoRI G'AATT_C
Cuts at: 3127 3505 3127
Size: 378 5440
EcoRV GAT'ATC
Cuts at: 2581 2581
Size: 5818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 5818
FspI TGC'GCA
Cuts at: 4324 4324
Size: 5818
HaeII r_GCGC'y
Cuts at: 1678 4965 1678
Size: 3287 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2116 2148 2150 2182 2525
Size: 32 135 32 544 32 2 32 343
Cuts at: 2525 2557 2650 2682 2853 2885 4459 4491 4533
Size: 32 93 32 171 32 1574 32 42
Cuts at: 4533 4565 1373
Size: 32 2626
Fragments arranged by size:
2626 1574 544 343 171 135 93 42
32 32 32 32 32 32 32 32
32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2539 3162 3408 669
Size: 988 281 601 623 246 3079
Fragments arranged by size:
3079 988 623 601 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5818
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 5694 5694
Size: 5818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2596 4932 5116 1305
Size: 1291 2336 184 2007
Fragments arranged by size:
2336 2007 1291 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 10
Size: 54 1425 343 390 3606
Fragments arranged by size:
3606 1425 390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2277 2295 2351 3776 4135 4294 518
Size: 648 1111 18 56 1425 359 159 2042
Fragments arranged by size:
2042 1425 1111 648 359 159 56 18
MspA1I CmG'CkG
Cuts at: 1606 3107 3234 3911 5059 5304 1606
Size: 1501 127 677 1148 245 2120
Fragments arranged by size:
2120 1501 1148 677 245 127
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5266
NdeI CA'TA_TG
Cuts at: 387 2655 2997 387
Size: 2268 342 3208
Fragments arranged by size:
3208 2268 342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 2383 2557 2936 3176 4721 1132
Size: 97 1154 174 379 240 1545 2229
Fragments arranged by size:
2229 1545 1154 379 240 174 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5818
PciI A'CATG_T
Cuts at: 2379 4717 2379
Size: 2338 3480
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2636 1108
Size: 1528 4290
PfoI T'CCnGG_A
Cuts at: 2503 2503
Size: 5818
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PmlI CAC'GTG
Cuts at: 2764 2764
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2044 2318 2044
Size: 274 5544
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PspXI vC'TCGA_Gb
Cuts at: 2329 2329
Size: 5818
PstI C_TGCA'G
Cuts at: 830 3170 830
Size: 2340 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
PvuII CAG'CTG
Cuts at: 3107 3107
Size: 5818
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SanDI GG'GwC_CC
Cuts at: 2044 2318 2044
Size: 274 5544
SapI GCTCTTCn'nnn_
Cuts at: 2943 2943
Size: 5818
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 4066 5778 4066
Size: 1712 4106
SfcI C'TryA_G
Cuts at: 826 1045 3068 3166 4301 4982 5173 826
Size: 219 2023 98 1135 681 191 1471
Fragments arranged by size:
2023 1471 1135 681 219 191 98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 3145 1872
Size: 1273 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2329 3922 4823 5085 820
Size: 197 926 386 1593 901 262 1553
Fragments arranged by size:
1593 1553 926 901 386 262 197
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 2936 3176 1132
Size: 1804 240 3774
Fragments arranged by size:
3774 1804 240
SspI AAT'ATT
Cuts at: 5 52 2963 3742 5
Size: 47 2911 779 2081
Fragments arranged by size:
2911 2081 779 47
StuI AGG'CCT
Cuts at: 1517 2304 1517
Size: 787 5031
StyI C'CwwG_G
Cuts at: 513 1065 2172 3257 513
Size: 552 1107 1085 3074
Fragments arranged by size:
3074 1107 1085 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2419 3845 3862 4015 4200 4645 325
Size: 1255 839 1426 17 153 185 445 1498
Fragments arranged by size:
1498 1426 1255 839 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2882 4064 5776
Size: 274 80 33 51 1443 905 1182 1712
Cuts at: 5776 96
Size: 138
Fragments arranged by size:
1712 1443 1182 905 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 5778 5810 2202
Size: 32 3544 32 2210
Fragments arranged by size:
3544 2210 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2637 2637
Size: 5818
XhoI C'TCGA_G
Cuts at: 1943 2329 1943
Size: 386 5432
XmaI C'CCGG_G
Cuts at: 1870 3143 1870
Size: 1273 4545
XmnI GAAnn'nnTTC
Cuts at: 3947 3947
Size: 5818
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PsrI RsrII SacI SacII SexAI SfiI SfoI
SrfI SwaI