Restriction map for
pFN21AB7665
(Circular) (Six-base) MAPSORT of: pFN21AB7665.seq Check: 2971 from: 1 to: 5716
pFN21AB7665 5716 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2586 2586
Size: 5716
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 2409 3037 2409
Size: 628 5088
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2297 2634 3779 5163 5431 1632
Size: 665 337 1145 1384 268 1917
Fragments arranged by size:
1917 1384 1145 665 337 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 3459 5031 3459
Size: 1572 4144
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2116 3025 3251 3403 5512 1493
Size: 623 909 226 152 2109 1697
Fragments arranged by size:
2109 1697 909 623 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 2083 3041 1870
Size: 73 140 958 4545
Fragments arranged by size:
4545 958 140 73
BamHI G'GATC_C
Cuts at: 2443 3046 3413 2443
Size: 603 367 4746
Fragments arranged by size:
4746 603 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2409 3037 4492 618
Size: 325 217 627 622 628 1455 1842
Fragments arranged by size:
1842 1455 628 627 622 325 217
BanII G_rGCy'C
Cuts at: 1391 2420 3035 1391
Size: 1029 615 4072
Fragments arranged by size:
4072 1029 615
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 928
Size: 971 328 4417
Fragments arranged by size:
4417 971 328
BbvCI CC'TCA_GC
Cuts at: 2949 2949
Size: 5716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2822 4327 136
Size: 107 122 71 631 605 1150 1505 1525
Fragments arranged by size:
1525 1505 1150 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 3777 4933 1790
Size: 1987 1156 2573
Fragments arranged by size:
2573 1987 1156
BmgBI CAC'GTC
Cuts at: 1318 2062 2940 1318
Size: 744 878 4094
Fragments arranged by size:
4094 878 744
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2566 3447 4405
Size: 416 53 83 399 24 1149 881 958
Cuts at: 4405 442
Size: 1753
Fragments arranged by size:
1753 1149 958 881 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 4376 1239
Size: 1340 1797 2579
Fragments arranged by size:
2579 1797 1340
Bpu10I CC'TnA_GC
Cuts at: 2414 2949 2414
Size: 535 5181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2045 4379 882
Size: 617 546 2334 2219
Fragments arranged by size:
2334 2219 617 546
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2182 3523 3905 4540
Size: 53 83 186 936 649 1341 382 635
Cuts at: 4540 275
Size: 1451
Fragments arranged by size:
1451 1341 936 649 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2402 2432 3000 3030 1178
Size: 30 165 30 999 30 568 30 3864
Fragments arranged by size:
3864 999 568 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2428 2672 1186
Size: 850 392 244 4230
Fragments arranged by size:
4230 850 392 244
BseYI C'CCAG_C
Cuts at: 2490 4919 5415 2490
Size: 2429 496 2791
Fragments arranged by size:
2791 2429 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2811 1163
Size: 573 1075 4068
Fragments arranged by size:
4068 1075 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2420 3777 3862 4933 2420
Size: 1357 85 1071 3203
Fragments arranged by size:
3203 1357 1071 85
BsmI GAATG_Cn'
Cuts at: 2727 2858 3227 3320 2727
Size: 131 369 93 5123
Fragments arranged by size:
5123 369 131 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2018 2018
Size: 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2420 3035 3777 3862 4933 1391
Size: 399 630 615 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 630 615 399 85
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 2367 3605 5335 2367
Size: 1238 1730 2748
Fragments arranged by size:
2748 1730 1238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2586 3071 844
Size: 1742 485 3489
Fragments arranged by size:
3489 1742 485
BsrBI CCG'CTC
Cuts at: 1394 1823 2542 3603 1394
Size: 429 719 1061 3507
Fragments arranged by size:
3507 1061 719 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4211 4385 65
Size: 1183 2963 174 1396
Fragments arranged by size:
2963 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4360 4547 1700
Size: 110 2550 187 2869
Fragments arranged by size:
2869 2550 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2062
Size: 5716
BstYI r'GATC_y
Cuts at: 1972 2252 2443 3046 3413 3798 3815 5267 5353
Size: 280 191 603 367 385 17 1452 86
Cuts at: 5353 5365 5711 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 603 385 367 346 280 191
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2358 2421 2520 2668 2730 2820 2898
Size: 552 1293 63 99 148 62 90 78
Cuts at: 2898 513
Size: 3331
Fragments arranged by size:
3331 1293 552 148 99 90 78 63
62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2898 2968 3232 4026 4046 2898
Size: 70 264 794 20 4568
Fragments arranged by size:
4568 794 264 70 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 2688 2895
Size: 54 1425 50 275 18 675 183 207
Cuts at: 2895 2901 3163 4052 5681 8
Size: 6 262 889 1629 43
Fragments arranged by size:
1629 1425 889 675 275 262 207 183
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3646 1353
Size: 2293 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2418 2418
Size: 5716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2579 2634 3779 4376 5163 5431
Size: 393 665 282 55 1145 597 787 268
Cuts at: 5431 1239
Size: 1524
Fragments arranged by size:
1524 1145 787 665 597 393 282 268
55
EcoRI G'AATT_C
Cuts at: 2116 3025 3403 2116
Size: 909 378 4429
Fragments arranged by size:
4429 909 378
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2014 4863 1678
Size: 336 2849 2531
Fragments arranged by size:
2849 2531 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2281 2313 4357 4389 4431
Size: 32 135 32 709 32 2044 32 42
Cuts at: 4431 4463 1373
Size: 32 2626
Fragments arranged by size:
2626 2044 709 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2625 3060 3306 669
Size: 988 281 687 435 246 3079
Fragments arranged by size:
3079 988 687 435 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 2413 3041 2413
Size: 628 5088
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2147 2986 4830 5014 1305
Size: 842 839 1844 184 2007
Fragments arranged by size:
2007 1844 842 839 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3894
Fragments arranged by size:
3894 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 3674 4033 4192 518
Size: 648 894 1614 359 159 2042
Fragments arranged by size:
2042 1614 894 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2449 2494 2822 2900 3132 3809 4957 5202
Size: 843 45 328 78 232 677 1148 245
Cuts at: 5202 1606
Size: 2120
Fragments arranged by size:
2120 1148 843 677 328 245 232 78
45
NcoI C'CATG_G
Cuts at: 513 1065 2358 2520 513
Size: 552 1293 162 3709
Fragments arranged by size:
3709 1293 552 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 3074 4619 1132
Size: 97 1845 1545 2229
Fragments arranged by size:
2229 1845 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2991 1348
Size: 1643 4073
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2066 2098 3785 3817 5298 5330 2066
Size: 32 1687 32 1481 32 2452
Fragments arranged by size:
2452 1687 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
SacI G_AGCT'C
Cuts at: 2420 2420
Size: 5716
SacII CC_GC'GG
Cuts at: 2823 2901 2823
Size: 78 5638
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 2095 3964 5676 2095
Size: 1869 1712 2135
Fragments arranged by size:
2135 1869 1712
SfcI C'TryA_G
Cuts at: 826 1045 3064 4199 4880 5071 826
Size: 219 2019 1135 681 191 1471
Fragments arranged by size:
2019 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3820 4721 4983 820
Size: 197 926 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 2280 3640 5
Size: 47 2228 1360 2081
Fragments arranged by size:
2228 2081 1360 47
StuI AGG'CCT
Cuts at: 1517 2430 1517
Size: 913 4803
StyI C'CwwG_G
Cuts at: 513 1065 2358 2520 3155 513
Size: 552 1293 162 635 3074
Fragments arranged by size:
3074 1293 635 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 3743 3760 3913 4098 4543 325
Size: 1255 924 1239 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1239 924 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2093 3962 5674
Size: 274 80 33 51 1443 116 1869 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1869 1712 1443 274 138 116 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2820 2852 2929 2961 5676 5708 2820
Size: 32 77 32 2715 32 2828
Fragments arranged by size:
2828 2715 77 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI