Restriction map for
pFN21AB7663
(Circular) (Six-base) MAPSORT of: pFN21AB7663.seq Check: 3993 from: 1 to: 5713
pFN21AB7663 5713 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3523 278
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2137 2876 3056 1937
Size: 200 739 180 4594
Fragments arranged by size:
4594 739 200 180
Acc65I G'GTAC_C
Cuts at: 3034 3034
Size: 5713
AclI AA'CG_TT
Cuts at: 1563 3840 4213 1563
Size: 2277 373 3063
Fragments arranged by size:
3063 2277 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2468 3776 5160 5428 1632
Size: 836 1308 1384 268 1917
Fragments arranged by size:
1917 1384 1308 836 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5516
AflIII A'CryG_T
Cuts at: 1203 4612 5589 1203
Size: 3409 977 1327
Fragments arranged by size:
3409 1327 977
AgeI A'CCGG_T
Cuts at: 4544 4544
Size: 5713
AhdI GACnn_n'nnGTC
Cuts at: 4442 4442
Size: 5713
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5713
AlwNI CAG_nnn'CTG
Cuts at: 3456 5028 3456
Size: 1572 4141
ApaLI G'TGCA_C
Cuts at: 2730 3770 4926 2730
Size: 1040 1156 3517
Fragments arranged by size:
3517 1156 1040
ApoI r'AATT_y
Cuts at: 1493 2340 3022 3248 3400 5509 1493
Size: 847 682 226 152 2109 1697
Fragments arranged by size:
2109 1697 847 682 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2150 4267 160
Size: 872 1118 2117 1606
Fragments arranged by size:
2117 1606 1118 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
AvaI C'yCGr_G
Cuts at: 1870 1943 3038 1870
Size: 73 1095 4545
Fragments arranged by size:
4545 1095 73
BamHI G'GATC_C
Cuts at: 3043 3410 3043
Size: 367 5346
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3034 4489 618
Size: 325 217 627 1247 1455 1842
Fragments arranged by size:
1842 1455 1247 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3032 1391
Size: 1641 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3904 3938 1903
Size: 34 1967 34 3678
Fragments arranged by size:
3678 1967 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3605 4815 3605
Size: 1210 4503
BclI T'GATC_A
Cuts at: 1548 2773 1548
Size: 1225 4488
BfrBI ATG'CAT
Cuts at: 2549 2549
Size: 5713
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4324 136
Size: 107 122 71 631 605 2652 1525
Fragments arranged by size:
2652 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5708 5708
Size: 5713
BlpI GC'TnA_GC
Cuts at: 3130 3130
Size: 5713
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2734 3774 4930 1790
Size: 369 575 1040 1156 2573
Fragments arranged by size:
2573 1156 1040 575 369
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5713
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3444 4402 442
Size: 416 53 83 399 24 2027 958 1753
Fragments arranged by size:
2027 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4373 1239
Size: 3134 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3838 4703 5001 3838
Size: 865 298 4550
Fragments arranged by size:
4550 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4376 882
Size: 617 2877 2219
Fragments arranged by size:
2877 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3504 493
Size: 711 2300 2702
Fragments arranged by size:
2702 2300 711
BsaBI GATnn'nnATC
Cuts at: 5707 5707
Size: 5713
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3520 3902 4537 275
Size: 53 83 186 936 1987 382 635 1451
Fragments arranged by size:
1987 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4146 4544 4818 4965 5376 4146
Size: 398 274 147 411 4483
Fragments arranged by size:
4483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5488
Fragments arranged by size:
5488 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5713
BseYI C'CCAG_C
Cuts at: 4916 5412 4916
Size: 496 5217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2241 1163
Size: 573 303 202 4635
Fragments arranged by size:
4635 573 303 202
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3163 3924 4952 1540
Size: 275 186 1162 761 1028 2301
Fragments arranged by size:
2301 1162 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2734 3009 3774 3859 4930 2734
Size: 275 765 85 1071 3517
Fragments arranged by size:
3517 1071 765 275 85
BsmI GAATG_Cn'
Cuts at: 3224 3317 3224
Size: 93 5620
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2734 3009 3032 3774 3859 4930
Size: 399 369 575 275 23 742 85 1071
Cuts at: 4930 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 575 399 369 275 85
23
BspEI T'CCGG_A
Cuts at: 5376 5376
Size: 5713
BspHI T'CATG_A
Cuts at: 3602 5332 3602
Size: 1730 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3068 844
Size: 2224 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3600 1394
Size: 429 1777 3507
Fragments arranged by size:
3507 1777 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2649 4208 4382 65
Size: 1183 1401 1559 174 1396
Fragments arranged by size:
1559 1401 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4357 4544 1700
Size: 110 2547 187 2869
Fragments arranged by size:
2869 2547 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5713
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5713
BssSI C'ACGA_G
Cuts at: 3773 4785 3773
Size: 1012 4701
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5713
BstBI TT'CG_AA
Cuts at: 2821 4517 2821
Size: 1696 4017
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5713
BstXI CCAn_nnnn'nTGG
Cuts at: 2713 2791 2713
Size: 78 5635
BstYI r'GATC_y
Cuts at: 1972 3043 3410 3795 3812 5264 5350 5362 5708
Size: 1071 367 385 17 1452 86 12 346
Cuts at: 5708 1972
Size: 1977
Fragments arranged by size:
1977 1452 1071 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2877 2877
Size: 5713
Bsu36I CC'TnA_GG
Cuts at: 2088 2088
Size: 5713
BtgI C'CryG_G
Cuts at: 513 1065 2976 513
Size: 552 1911 3250
Fragments arranged by size:
3250 1911 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4974
Fragments arranged by size:
4974 683 56
BtsI GCAGTG_nn'
Cuts at: 2536 3229 4023 4043 2536
Size: 693 794 20 4206
Fragments arranged by size:
4206 794 693 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5678
DraI TTT'AAA
Cuts at: 3017 3017
Size: 5713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4720 809
Size: 507 3404 1802
Fragments arranged by size:
3404 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2902 3160 4049
Size: 54 1425 50 275 18 1072 258 889
Cuts at: 4049 5678 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1072 889 275 258 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3160 1537
Size: 275 1348 4090
Fragments arranged by size:
4090 1348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3643 1353
Size: 2290 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4300 4674 4820 387
Size: 1064 138 2711 374 146 1280
Fragments arranged by size:
2711 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 3776 4373 5160 5428 1239
Size: 393 836 1308 597 787 268 1524
Fragments arranged by size:
1524 1308 836 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2576 2576
Size: 5713
EcoRI G'AATT_C
Cuts at: 3022 3400 3022
Size: 378 5335
FspI TGC'GCA
Cuts at: 4219 4219
Size: 5713
HaeII r_GCGC'y
Cuts at: 1678 4860 1678
Size: 3182 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2765 2797 4354 4386 4428
Size: 32 135 32 1193 32 1557 32 42
Cuts at: 4428 4460 1373
Size: 32 2626
Fragments arranged by size:
2626 1557 1193 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3057 3303 669
Size: 988 281 1119 246 3079
Fragments arranged by size:
3079 1119 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5713
HpaI GTT'AAC
Cuts at: 3303 3303
Size: 5713
KpnI G_GTAC'C
Cuts at: 3038 3038
Size: 5713
MfeI C'AATT_G
Cuts at: 2649 3312 2649
Size: 663 5050
MluI A'CGCG_T
Cuts at: 5589 5589
Size: 5713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2255 4827 5011 1305
Size: 950 2572 184 2007
Fragments arranged by size:
2572 2007 950 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2904 10
Size: 54 1425 343 1072 2819
Fragments arranged by size:
2819 1425 1072 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2711 3671 4030 4189 518
Size: 648 1545 960 359 159 2042
Fragments arranged by size:
2042 1545 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3129 3806 4954 5199 1606
Size: 1523 677 1148 245 2120
Fragments arranged by size:
2120 1523 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2976 513
Size: 552 1911 3250
Fragments arranged by size:
3250 1911 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5713
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5713
NotI GC'GGCC_GC
Cuts at: 3160 3160
Size: 5713
NsiI A_TGCA'T
Cuts at: 2551 2551
Size: 5713
NspI r_CATG'y
Cuts at: 1132 1229 3071 4616 1132
Size: 97 1842 1545 2229
Fragments arranged by size:
2229 1842 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5713
PciI A'CATG_T
Cuts at: 4612 4612
Size: 5713
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5713
PmeI GTTT'AAAC
Cuts at: 3017 3017
Size: 5713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 5295 5327 3782
Size: 32 1481 32 4168
Fragments arranged by size:
4168 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5713
PsiI TTA'TAA
Cuts at: 3283 3283
Size: 5713
PstI C_TGCA'G
Cuts at: 830 2475 2543 3065 830
Size: 1645 68 522 3478
Fragments arranged by size:
3478 1645 522 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5713
SalI G'TCGA_C
Cuts at: 1936 3055 1936
Size: 1119 4594
SbfI CC_TGCA'GG
Cuts at: 3065 3065
Size: 5713
ScaI AGT'ACT
Cuts at: 3961 5673 3961
Size: 1712 4001
SfcI C'TryA_G
Cuts at: 826 1045 2471 2539 3061 4196 4877 5068 826
Size: 219 1426 68 522 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 681 522 219 191 68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5713
SmaI CCC'GGG
Cuts at: 1872 3040 1872
Size: 1168 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3817 4718 4980 820
Size: 197 926 1874 901 262 1553
Fragments arranged by size:
1874 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3504 493
Size: 3011 2702
SpeI A'CTAG_T
Cuts at: 152 2284 152
Size: 2132 3581
SphI G_CATG'C
Cuts at: 1132 3071 1132
Size: 1939 3774
SspI AAT'ATT
Cuts at: 5 52 3637 5
Size: 47 3585 2081
Fragments arranged by size:
3585 2081 47
StuI AGG'CCT
Cuts at: 1517 2698 1517
Size: 1181 4532
StyI C'CwwG_G
Cuts at: 513 1065 2976 3152 513
Size: 552 1911 176 3074
Fragments arranged by size:
3074 1911 552 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3740 3757 3910 4095 4540 325
Size: 1255 2160 17 153 185 445 1498
Fragments arranged by size:
2160 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3959 5671 96
Size: 274 80 33 51 1443 1982 1712 138
Fragments arranged by size:
1982 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5673 5705 5673
Size: 32 5681
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5713
XbaI T'CTAG_A
Cuts at: 3049 3049
Size: 5713
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5713
XmaI C'CCGG_G
Cuts at: 1870 3038 1870
Size: 1168 4545
XmnI GAAnn'nnTTC
Cuts at: 2817 3842 2817
Size: 1025 4688
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3521 276
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI