Restriction map for
pFN21AB7662
(Circular) (Six-base) MAPSORT of: pFN21AB7662.seq Check: 4036 from: 1 to: 5893
pFN21AB7662 5893 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2506 2506
Size: 5893
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2284 3236 1937
Size: 347 952 4594
Fragments arranged by size:
4594 952 347
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 3956 5340 5608 1632
Size: 477 1847 1384 268 1917
Fragments arranged by size:
1917 1847 1384 477 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5893
AlwNI CAG_nnn'CTG
Cuts at: 2321 3636 5208 2321
Size: 1315 1572 3006
Fragments arranged by size:
3006 1572 1315
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 2527 2660 2702 2920 3202 3428 3580 5689
Size: 1034 133 42 218 282 226 152 2109
Cuts at: 5689 1493
Size: 1697
Fragments arranged by size:
2109 1697 1034 282 226 218 152 133
42
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 2511 3218 1870
Size: 73 568 707 4545
Fragments arranged by size:
4545 707 568 73
BamHI G'GATC_C
Cuts at: 2010 3223 3590 2010
Size: 1213 367 4313
Fragments arranged by size:
4313 1213 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2354 3214 4669 618
Size: 325 217 627 567 860 1455 1842
Fragments arranged by size:
1842 1455 860 627 567 325 217
BanII G_rGCy'C
Cuts at: 1391 2136 2520 3212 1391
Size: 745 384 692 4072
Fragments arranged by size:
4072 745 692 384
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2499 928
Size: 971 600 4322
Fragments arranged by size:
4322 971 600
BbvCI CC'TCA_GC
Cuts at: 2039 2039
Size: 5893
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4084 4118 1903
Size: 34 2147 34 3678
Fragments arranged by size:
3678 2147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4504 136
Size: 107 122 71 631 605 2832 1525
Fragments arranged by size:
2832 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 3310 3310
Size: 5893
Bme1580I G_kGCm'C
Cuts at: 1790 2614 3954 5110 1790
Size: 824 1340 1156 2573
Fragments arranged by size:
2573 1340 1156 824
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2009 3624 4582
Size: 416 53 83 399 24 592 1615 958
Cuts at: 4582 442
Size: 1753
Fragments arranged by size:
1753 1615 958 592 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 4553 1239
Size: 1085 2229 2579
Fragments arranged by size:
2579 2229 1085
Bpu10I CC'TnA_GC
Cuts at: 2039 2039
Size: 5893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3090 4018 4883 5181 3090
Size: 928 865 298 3802
Fragments arranged by size:
3802 928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2243 4556 882
Size: 617 744 2313 2219
Fragments arranged by size:
2313 2219 744 617
BsaAI yAC'GTr
Cuts at: 493 1204 2227 3684 493
Size: 711 1023 1457 2702
Fragments arranged by size:
2702 1457 1023 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2195 3700 4082 4717
Size: 53 83 186 936 662 1505 382 635
Cuts at: 4717 275
Size: 1451
Fragments arranged by size:
1505 1451 936 662 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2167 4326 4724 4998 5145 5556 2167
Size: 2159 398 274 147 411 2504
Fragments arranged by size:
2504 2159 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2792 2822 1178
Size: 30 165 30 1389 30 4249
Fragments arranged by size:
4249 1389 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2134 1186
Size: 948 4945
BseYI C'CCAG_C
Cuts at: 5096 5592 5096
Size: 496 5397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2777 1163
Size: 573 1041 4279
Fragments arranged by size:
4279 1041 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2236 3343 4104 5132 1540
Size: 275 186 235 1107 761 1028 2301
Fragments arranged by size:
2301 1107 1028 761 275 235 186
BsiHKAI G_wGCw'C
Cuts at: 3954 4039 5110 3954
Size: 85 1071 4737
Fragments arranged by size:
4737 1071 85
BsmI GAATG_Cn'
Cuts at: 3404 3497 3404
Size: 93 5800
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2520 2614 3212 3954 4039 5110
Size: 399 346 384 94 598 742 85 1071
Cuts at: 5110 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 598 399 384 346 94
85
BspEI T'CCGG_A
Cuts at: 5556 5556
Size: 5893
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2506 3248 844
Size: 1662 742 3489
Fragments arranged by size:
3489 1662 742
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4388 4562 65
Size: 1183 3140 174 1396
Fragments arranged by size:
3140 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2113 4537 4724 1700
Size: 110 303 2424 187 2869
Fragments arranged by size:
2869 2424 303 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 2010 2467 2722 3223 3590 3975 3992 5444
Size: 38 457 255 501 367 385 17 1452
Cuts at: 5444 5530 5542 5888 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 501 457 385 367 346 255
86 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2410 2410
Size: 5893
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 3409 4203 4223 3409
Size: 794 20 5079
Fragments arranged by size:
5079 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 2461 2571 3197 2461
Size: 110 626 5157
Fragments arranged by size:
5157 626 110
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858
Size: 54 1425 50 275 18 1510 889 1629
Cuts at: 5858 8
Size: 43
Fragments arranged by size:
1629 1510 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3823 1353
Size: 2470 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2038 2172 4480 4854 5000 387
Size: 1064 138 449 134 2308 374 146 1280
Fragments arranged by size:
2308 1280 1064 449 374 146 138 134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2324 3956 4553 5340 5608 1239
Size: 393 477 215 1632 597 787 268 1524
Fragments arranged by size:
1632 1524 787 597 477 393 268 215
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2333
Size: 5893
EcoO109I rG'GnC_Cy
Cuts at: 2318 2318
Size: 5893
EcoRI G'AATT_C
Cuts at: 2702 3202 3580 2702
Size: 500 378 5015
Fragments arranged by size:
5015 500 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 5893
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 2064 3003 5040 1678
Size: 386 939 2037 2531
Fragments arranged by size:
2531 2037 939 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2792 2824 2899 2931 4534
Size: 32 135 32 1220 32 75 32 1603
Cuts at: 4534 4566 4608 4640 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1603 1220 135 75 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3094 3237 3483 669
Size: 988 281 1156 143 246 3079
Fragments arranged by size:
3079 1156 988 281 246 143
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5893
HpaI GTT'AAC
Cuts at: 3094 3483 3094
Size: 389 5504
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2560 5007 5191 1305
Size: 1255 2447 184 2007
Fragments arranged by size:
2447 2007 1255 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3851 4210 4369 518
Size: 648 2685 359 159 2042
Fragments arranged by size:
2685 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2155 2842 3309 3986 5134 5379 1606
Size: 549 687 467 677 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 549 467 245
NaeI GCC'GGC
Cuts at: 2115 2115
Size: 5893
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5341
NdeI CA'TA_TG
Cuts at: 387 2629 387
Size: 2242 3651
NgoMIV G'CCGG_C
Cuts at: 2113 2113
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 3100 3251 4796 1132
Size: 97 1871 151 1545 2229
Fragments arranged by size:
2229 1871 1545 151 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2321 1108
Size: 1213 4680
PfoI T'CCnGG_A
Cuts at: 2320 2725 2320
Size: 405 5488
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2318 2318
Size: 5893
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5893
PsiI TTA'TAA
Cuts at: 3159 3463 3159
Size: 304 5589
PstI C_TGCA'G
Cuts at: 830 3245 830
Size: 2415 3478
PvuI CG_AT'CG
Cuts at: 2001 2236 2001
Size: 235 5658
PvuII CAG'CTG
Cuts at: 2155 2155
Size: 5893
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SfcI C'TryA_G
Cuts at: 826 1045 3132 3241 4376 5057 5248 826
Size: 219 2087 109 1135 681 191 1471
Fragments arranged by size:
2087 1471 1135 681 219 191 109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2511 3105 3997 4898 5160 820
Size: 197 926 568 594 892 901 262 1553
Fragments arranged by size:
1553 926 901 892 594 568 262 197
SnaBI TAC'GTA
Cuts at: 493 2227 3684 493
Size: 1734 1457 2702
Fragments arranged by size:
2702 1734 1457
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 2052 2186 2549 2804 3332 513
Size: 552 987 134 363 255 528 3074
Fragments arranged by size:
3074 987 552 528 363 255 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3920 3937 4090 4275 4720 325
Size: 1255 2340 17 153 185 445 1498
Fragments arranged by size:
2340 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2645 4139 5851
Size: 274 80 33 51 1443 668 1494 1712
Cuts at: 5851 96
Size: 138
Fragments arranged by size:
1712 1494 1443 668 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2113 2145 5853 5885 2113
Size: 32 3708 32 2121
Fragments arranged by size:
3708 2121 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 2511 1943
Size: 568 5325
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 2544 4022 2544
Size: 1478 4415
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI