Restriction map for
pFN21AB7658
(Circular) (Six-base) MAPSORT of: pFN21AB7658.seq Check: 8973 from: 1 to: 5887
pFN21AB7658 5887 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2706 3230 1937
Size: 769 524 4594
Fragments arranged by size:
4594 769 524
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2508 2529 3950 5334 5602 1632
Size: 596 280 21 1421 1384 268 1917
Fragments arranged by size:
1917 1421 1384 596 280 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 4786 5763 1203
Size: 3583 977 1327
Fragments arranged by size:
3583 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 3630 5202 3630
Size: 1572 4315
ApaI G_GGCC'C
Cuts at: 2121 2121
Size: 5887
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 2901 2959 3196 3422 3574 5683 1493
Size: 1408 58 237 226 152 2109 1697
Fragments arranged by size:
2109 1697 1408 237 226 152 58
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 3212 1870
Size: 73 1269 4545
Fragments arranged by size:
4545 1269 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2746 2779 2746
Size: 33 5854
BamHI G'GATC_C
Cuts at: 2404 3217 3584 2404
Size: 813 367 4707
Fragments arranged by size:
4707 813 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3208 4663 618
Size: 325 217 627 1421 1455 1842
Fragments arranged by size:
1842 1455 1421 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 2237 3206 1391
Size: 730 116 969 4072
Fragments arranged by size:
4072 969 730 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2535 928
Size: 971 636 4280
Fragments arranged by size:
4280 971 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4498 136
Size: 107 122 71 631 605 337 2489 1525
Fragments arranged by size:
2489 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 2121 3948 5104 1790
Size: 331 1827 1156 2573
Fragments arranged by size:
2573 1827 1156 331
BmgBI CAC'GTC
Cuts at: 1318 2424 1318
Size: 1106 4781
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 3618 4576
Size: 416 53 83 399 24 767 1434 958
Cuts at: 4576 442
Size: 1753
Fragments arranged by size:
1753 1434 958 767 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2847 4547 1239
Size: 926 682 1700 2579
Fragments arranged by size:
2579 1700 926 682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 4012 4877 5175 2221
Size: 1791 865 298 2933
Fragments arranged by size:
2933 1791 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2201 2581 3079 4550 882
Size: 617 702 380 498 1471 2219
Fragments arranged by size:
2219 1471 702 617 498 380
BsaAI yAC'GTr
Cuts at: 493 1204 3678 493
Size: 711 2474 2702
Fragments arranged by size:
2702 2474 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3694 4076 4711 275
Size: 53 83 186 936 2161 382 635 1451
Fragments arranged by size:
2161 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5662
Fragments arranged by size:
5662 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 2670 3062 1186
Size: 1171 313 392 4011
Fragments arranged by size:
4011 1171 392 313
BseYI C'CCAG_C
Cuts at: 2083 5090 5586 2083
Size: 3007 496 2384
Fragments arranged by size:
3007 2384 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5314
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2237 3948 4033 5104 2151
Size: 86 1711 85 1071 2934
Fragments arranged by size:
2934 1711 1071 86 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2151 2237 3206 3948 4033 5104
Size: 399 331 30 86 969 742 85 1071
Cuts at: 5104 1391
Size: 2174
Fragments arranged by size:
2174 1071 969 742 399 331 86 85
30
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 2977 3073 3776 5506 2977
Size: 96 703 1730 3358
Fragments arranged by size:
3358 1730 703 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3242 844
Size: 2398 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5887
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstYI r'GATC_y
Cuts at: 1972 2404 2497 3217 3584 3969 3986 5438 5524
Size: 432 93 720 367 385 17 1452 86
Cuts at: 5524 5536 5882 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 720 432 385 367 346 93
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2340 2340
Size: 5887
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 2377 2452 3403 4197 4217 2377
Size: 75 951 794 20 4047
Fragments arranged by size:
4047 951 794 75 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3088 3123 529
Size: 35 2524 35 3293
Fragments arranged by size:
3293 2524 35 35
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DraIII CAC_nnn'GTG
Cuts at: 2771 2771
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2447 4894 809
Size: 507 1131 2447 1802
Fragments arranged by size:
2447 1802 1131 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852
Size: 54 1425 50 275 18 1504 889 1629
Cuts at: 5852 8
Size: 43
Fragments arranged by size:
1629 1504 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3817 1353
Size: 2464 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2058 4474 4848 4994 387
Size: 1064 138 469 2416 374 146 1280
Fragments arranged by size:
2416 1280 1064 469 374 146 138
EcoICRI GAG'CTC
Cuts at: 2235 2235
Size: 5887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2228 2508 2529 2847 3950 4547
Size: 393 533 63 280 21 318 1103 597
Cuts at: 4547 5334 5602 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1103 787 597 533 393 318 280
268 63 21
EcoO109I rG'GnC_Cy
Cuts at: 2817 2817
Size: 5887
EcoRI G'AATT_C
Cuts at: 2901 2959 3196 3574 2901
Size: 58 237 378 5214
Fragments arranged by size:
5214 378 237 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2114 2227 2068
Size: 46 113 5728
Fragments arranged by size:
5728 113 46
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 5034 1678
Size: 3356 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2219 2251 2300 2302 2332
Size: 32 135 32 647 32 49 2 30
Cuts at: 2332 2334 2389 2421 4528 4560 4602 4634 1373
Size: 2 55 32 2107 32 42 32 2626
Fragments arranged by size:
2626 2107 647 135 55 49 42 32
32 32 32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2276 2863 748
Size: 1528 587 3772
Fragments arranged by size:
3772 1528 587
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4065
Fragments arranged by size:
4065 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3845 4204 4363 518
Size: 648 2679 359 159 2042
Fragments arranged by size:
2679 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3303 3980 5128 5373 1606
Size: 1697 677 1148 245 2120
Fragments arranged by size:
2120 1697 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5335
NdeI CA'TA_TG
Cuts at: 387 3091 387
Size: 2704 3183
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 3245 4790 1132
Size: 97 2016 1545 2229
Fragments arranged by size:
2229 2016 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2771 3023 1108
Size: 1663 252 3972
Fragments arranged by size:
3972 1663 252
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2817 2817
Size: 5887
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PspOMI G'GGCC_C
Cuts at: 2117 2117
Size: 5887
PstI C_TGCA'G
Cuts at: 830 3069 3239 830
Size: 2239 170 3478
Fragments arranged by size:
3478 2239 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
SacI G_AGCT'C
Cuts at: 2237 2237
Size: 5887
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SfcI C'TryA_G
Cuts at: 826 1045 2514 3065 3235 4370 5051 5242 826
Size: 219 1469 551 170 1135 681 191 1471
Fragments arranged by size:
1471 1469 1135 681 551 219 191 170
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2236 3991 4892 5154 820
Size: 197 926 293 1755 901 262 1553
Fragments arranged by size:
1755 1553 926 901 293 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5887
StyI C'CwwG_G
Cuts at: 513 1065 2211 2268 2571 3326 513
Size: 552 1146 57 303 755 3074
Fragments arranged by size:
3074 1146 755 552 303 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3914 3931 4084 4269 4714 325
Size: 1255 2334 17 153 185 445 1498
Fragments arranged by size:
2334 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4133 5845 96
Size: 274 80 33 51 1443 2156 1712 138
Fragments arranged by size:
2156 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 2314 1316
Size: 998 4889
XbaI T'CTAG_A
Cuts at: 2683 3223 2683
Size: 540 5347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 2176
Size: 5887
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 2864 3032 4016 2864
Size: 168 984 4735
Fragments arranged by size:
4735 984 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI