Restriction map for
pFN21AB7656
(Circular) (Six-base) MAPSORT of: pFN21AB7656.seq Check: 6432 from: 1 to: 5887
pFN21AB7656 5887 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3230 1937
Size: 1293 4594
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 3950 5334 5602 1632
Size: 812 1506 1384 268 1917
Fragments arranged by size:
1917 1506 1384 812 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 4786 5763 1203
Size: 3583 977 1327
Fragments arranged by size:
3583 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 2557 3630 5202 2557
Size: 1073 1572 3242
Fragments arranged by size:
3242 1572 1073
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 2210 2290 2429 3196 3422 3574 5683 1493
Size: 717 80 139 767 226 152 2109 1697
Fragments arranged by size:
2109 1697 767 717 226 152 139 80
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 2921 3143 3212 1870
Size: 73 978 222 69 4545
Fragments arranged by size:
4545 978 222 73 69
BamHI G'GATC_C
Cuts at: 3217 3584 3217
Size: 367 5520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3156 3208 4663 618
Size: 325 217 627 1369 52 1455 1842
Fragments arranged by size:
1842 1455 1369 627 325 217 52
BanII G_rGCy'C
Cuts at: 1391 3206 1391
Size: 1815 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4078 4112 1903
Size: 34 50 34 2057 34 3678
Fragments arranged by size:
3678 2057 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 2469 1548
Size: 921 4966
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2419 3180 4498
Size: 107 122 71 631 605 747 761 1318
Cuts at: 4498 136
Size: 1525
Fragments arranged by size:
1525 1318 761 747 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2107 5882 2107
Size: 3775 2112
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 3948 5104 1790
Size: 2158 1156 2573
Fragments arranged by size:
2573 2158 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5887
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2563 2998 3618
Size: 416 53 83 399 24 1146 435 620
Cuts at: 3618 4576 442
Size: 958 1753
Fragments arranged by size:
1753 1146 958 620 435 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2467 2792 4547 1239
Size: 1228 325 1755 2579
Fragments arranged by size:
2579 1755 1228 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 4012 4877 5175 2132
Size: 1880 865 298 2844
Fragments arranged by size:
2844 1880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2613 4550 882
Size: 617 1114 1937 2219
Fragments arranged by size:
2219 1937 1114 617
BsaAI yAC'GTr
Cuts at: 493 1204 2158 3678 493
Size: 711 954 1520 2702
Fragments arranged by size:
2702 1520 954 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3694 4076 4711 275
Size: 53 83 186 936 2161 382 635 1451
Fragments arranged by size:
2161 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4320 4718 4992 5139 5550 4320
Size: 398 274 147 411 4657
Fragments arranged by size:
4657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5662
Fragments arranged by size:
5662 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3067 1186
Size: 1881 4006
BseYI C'CCAG_C
Cuts at: 5090 5586 5090
Size: 496 5391
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5314
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3337 4098 5126 1540
Size: 275 186 1336 761 1028 2301
Fragments arranged by size:
2301 1336 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3948 4033 5104 3948
Size: 85 1071 4731
Fragments arranged by size:
4731 1071 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3206 3948 4033 5104 1391
Size: 399 1416 742 85 1071 2174
Fragments arranged by size:
2174 1416 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 2961 3030 3776 5506 2961
Size: 69 746 1730 3342
Fragments arranged by size:
3342 1730 746 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2962 3242 844
Size: 2118 280 3489
Fragments arranged by size:
3489 2118 280
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2318 4382 4556 65
Size: 1183 1070 2064 174 1396
Fragments arranged by size:
2064 1396 1183 1070 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 2746 96
Size: 2650 3237
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 2735 3947 4959 2735
Size: 1212 1012 3663
Fragments arranged by size:
3663 1212 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2320 794
Size: 1526 4361
BstBI TT'CG_AA
Cuts at: 2260 4691 2260
Size: 2431 3456
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstYI r'GATC_y
Cuts at: 1972 2107 3217 3584 3969 3986 5438 5524 5536
Size: 135 1110 367 385 17 1452 86 12
Cuts at: 5536 5882 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1110 385 367 346 135 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5335
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 2016 2731 3403 4197 4217 2016
Size: 715 672 794 20 3686
Fragments arranged by size:
3686 794 715 672 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 2053 3132 3191 2053
Size: 1079 59 4749
Fragments arranged by size:
4749 1079 59
DraIII CAC_nnn'GTG
Cuts at: 2755 2755
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3172 3181 3334
Size: 54 1425 50 275 18 1342 9 153
Cuts at: 3334 4223 5852 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1342 889 275 153 54 50
43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2020 3029 3069 3075 3081 3093 3817 1353
Size: 667 1009 40 6 6 12 724 3423
Fragments arranged by size:
3423 1009 724 667 40 12 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2413 4474 4848 4994 387
Size: 1064 138 824 2061 374 146 1280
Fragments arranged by size:
2061 1280 1064 824 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 2467 2792 3950 4547 5334 5602
Size: 393 812 23 325 1158 597 787 268
Cuts at: 5602 1239
Size: 1524
Fragments arranged by size:
1524 1158 812 787 597 393 325 268
23
EcoO109I rG'GnC_Cy
Cuts at: 2730 2730
Size: 5887
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 5887
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 2371 5034 1678
Size: 693 2663 2531
Fragments arranged by size:
2663 2531 693
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4528 4560 4602 4634 1373
Size: 32 135 32 2956 32 42 32 2626
Fragments arranged by size:
2956 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2439 748
Size: 1691 4196
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 2114 5001 5185 1305
Size: 744 65 2887 184 2007
Fragments arranged by size:
2887 2007 744 184 65
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3174 3183 10
Size: 54 1425 343 1342 9 2714
Fragments arranged by size:
2714 1425 1342 343 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 3183 3845 4204 4363 518
Size: 648 2017 662 359 159 2042
Fragments arranged by size:
2042 2017 662 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3303 3980 5128 5373 1606
Size: 1697 677 1148 245 2120
Fragments arranged by size:
2120 1697 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5335
NdeI CA'TA_TG
Cuts at: 387 2170 387
Size: 1783 4104
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 3245 4790 1132
Size: 97 2016 1545 2229
Fragments arranged by size:
2229 2016 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2674 1108
Size: 1566 4321
PfoI T'CCnGG_A
Cuts at: 2433 3042 2433
Size: 609 5278
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2730 2730
Size: 5887
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PspXI vC'TCGA_Gb
Cuts at: 2921 2921
Size: 5887
PstI C_TGCA'G
Cuts at: 830 3239 830
Size: 2409 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SanDI GG'GwC_CC
Cuts at: 2730 2730
Size: 5887
SapI GCTCTTCn'nnn_
Cuts at: 2020 3029 2020
Size: 1009 4878
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 2573 4135 5847 2573
Size: 1562 1712 2613
Fragments arranged by size:
2613 1712 1562
SfcI C'TryA_G
Cuts at: 826 1045 2954 3235 4370 5051 5242 826
Size: 219 1909 281 1135 681 191 1471
Fragments arranged by size:
1909 1471 1135 681 281 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3180 3180
Size: 5887
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 2921 3991 4892 5154 820
Size: 197 926 168 810 1070 901 262 1553
Fragments arranged by size:
1553 1070 926 901 810 262 197 168
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 2413 1517
Size: 896 4991
StyI C'CwwG_G
Cuts at: 513 1065 2507 3326 513
Size: 552 1442 819 3074
Fragments arranged by size:
3074 1442 819 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3914 3931 4084 4269 4714 325
Size: 1255 2334 17 153 185 445 1498
Fragments arranged by size:
2334 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2571 2746 4133
Size: 274 80 33 51 1443 594 175 1387
Cuts at: 4133 5845 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1387 594 274 175 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2097 2571 2097
Size: 474 5413
XhoI C'TCGA_G
Cuts at: 1943 2921 1943
Size: 978 4909
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 2294 2429 4016 2294
Size: 135 1587 4165
Fragments arranged by size:
4165 1587 135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI
PvuII RsrII SacI SacII SexAI SfoI SrfI SwaI