Restriction map for
pFN21AB7654
(Circular) (Six-base) MAPSORT of: pFN21AB7654.seq Check: 1061 from: 1 to: 5818
pFN21AB7654 5818 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2699 2699
Size: 5818
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3161 1937
Size: 1224 4594
Acc65I G'GTAC_C
Cuts at: 2134 3139 2134
Size: 1005 4813
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 3881 5265 5533 1632
Size: 932 1317 1384 268 1917
Fragments arranged by size:
1917 1384 1317 932 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 2461 4717 5694 1203
Size: 1258 2256 977 1327
Fragments arranged by size:
2256 1327 1258 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 4547 4547
Size: 5818
AleI CACnn'nnGTG
Cuts at: 1166 2881 1166
Size: 1715 4103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2370 1184
Size: 1186 4632
AlwNI CAG_nnn'CTG
Cuts at: 2599 2691 3561 5133 2599
Size: 92 870 1572 3284
Fragments arranged by size:
3284 1572 870 92
ApaI G_GGCC'C
Cuts at: 2104 2104
Size: 5818
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 2451 2611 3127 3353 3505 5614 1493
Size: 958 160 516 226 152 2109 1697
Fragments arranged by size:
2109 1697 958 516 226 160 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 3143 1870
Size: 73 1200 4545
Fragments arranged by size:
4545 1200 73
BamHI G'GATC_C
Cuts at: 3148 3515 3148
Size: 367 5451
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2134 3139 4594 618
Size: 325 217 627 293 54 1005 1455 1842
Fragments arranged by size:
1842 1455 1005 627 325 293 217 54
BanII G_rGCy'C
Cuts at: 1391 2104 3137 1391
Size: 713 1033 4072
Fragments arranged by size:
4072 1033 713
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4847
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4009 4043 1903
Size: 34 2072 34 3678
Fragments arranged by size:
3678 2072 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5818
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 4429 136
Size: 107 122 71 631 605 427 2330 1525
Fragments arranged by size:
2330 1525 631 605 427 122 107 71
BglII A'GATC_T
Cuts at: 2489 5813 2489
Size: 3324 2494
BlpI GC'TnA_GC
Cuts at: 2775 3235 2775
Size: 460 5358
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2809 3879 5035 1790
Size: 314 705 1070 1156 2573
Fragments arranged by size:
2573 1156 1070 705 314
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5818
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2122 2155 2808
Size: 416 53 83 399 24 705 33 653
Cuts at: 2808 2955 3549 4507 442
Size: 147 594 958 1753
Fragments arranged by size:
1753 958 705 653 594 416 399 147
83 53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2621 2627 2855 2894 2999 4478 1239
Size: 1382 6 228 39 105 1479 2579
Fragments arranged by size:
2579 1479 1382 228 105 39 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2481 3943 4808 5106 2481
Size: 1462 865 298 3193
Fragments arranged by size:
3193 1462 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4481 882
Size: 617 2982 2219
Fragments arranged by size:
2982 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3609 493
Size: 711 2405 2702
Fragments arranged by size:
2702 2405 711
BsaBI GATnn'nnATC
Cuts at: 5812 5812
Size: 5818
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3625 4007 4642 275
Size: 53 83 186 936 2092 382 635 1451
Fragments arranged by size:
2092 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4251 4649 4923 5070 5481 4251
Size: 398 274 147 411 4588
Fragments arranged by size:
4588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 1178
Size: 30 165 30 828 30 4735
Fragments arranged by size:
4735 828 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2200 2348 2393 2714 2737 1186
Size: 1014 148 45 321 23 4267
Fragments arranged by size:
4267 1014 321 148 45 23
BseYI C'CCAG_C
Cuts at: 2185 5021 5517 2185
Size: 2836 496 2486
Fragments arranged by size:
2836 2486 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2985 1163
Size: 573 1249 3996
Fragments arranged by size:
3996 1249 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3268 4029 5057 1540
Size: 275 186 1267 761 1028 2301
Fragments arranged by size:
2301 1267 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2171 3879 3964 5035 2171
Size: 1708 85 1071 2954
Fragments arranged by size:
2954 1708 1071 85
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2171 2809 3137 3879 3964 5035
Size: 399 314 67 638 328 742 85 1071
Cuts at: 5035 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 638 399 328 314 85
67
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 2699 2743 3173 844
Size: 1202 653 44 430 3489
Fragments arranged by size:
3489 1202 653 430 44
BsrBI CCG'CTC
Cuts at: 1394 1823 3705 1394
Size: 429 1882 3507
Fragments arranged by size:
3507 1882 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4313 4487 65
Size: 1183 3065 174 1396
Fragments arranged by size:
3065 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4462 4649 1700
Size: 110 2652 187 2869
Fragments arranged by size:
2869 2652 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5818
BssSI C'ACGA_G
Cuts at: 3878 4890 3878
Size: 1012 4806
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2691 2947 794
Size: 1897 256 3665
Fragments arranged by size:
3665 1897 256
BstBI TT'CG_AA
Cuts at: 2479 4622 2479
Size: 2143 3675
BstEII G'GTnAC_C
Cuts at: 1184 2027 1184
Size: 843 4975
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2112 2918 2011
Size: 101 806 4911
Fragments arranged by size:
4911 806 101
BstYI r'GATC_y
Cuts at: 1972 2275 2489 3148 3515 3900 3917 5369 5455
Size: 303 214 659 367 385 17 1452 86
Cuts at: 5455 5467 5813 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 659 385 367 346 303 214
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2952 2952
Size: 5818
BtgI C'CryG_G
Cuts at: 513 1065 2004 2255 2649 513
Size: 552 939 251 394 3682
Fragments arranged by size:
3682 939 552 394 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5079
Fragments arranged by size:
5079 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2984 3265 4154
Size: 54 1425 50 275 18 1154 281 889
Cuts at: 4154 5783 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1154 889 281 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3265 1537
Size: 275 1453 4090
Fragments arranged by size:
4090 1453 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2378 3005 3748 1353
Size: 1025 627 743 3423
Fragments arranged by size:
3423 1025 743 627
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4405 4779 4925 387
Size: 1064 138 2816 374 146 1280
Fragments arranged by size:
2816 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2564 2621 2627 2855 2894 2999 3881
Size: 393 932 57 6 228 39 105 882
Cuts at: 3881 4478 5265 5533 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 932 882 787 597 393 268 228
105 57 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 2054
Size: 5818
EcoO109I rG'GnC_Cy
Cuts at: 2100 2101 2307 2821 2914 3023 2100
Size: 1 206 514 93 109 4895
Fragments arranged by size:
4895 514 206 109 93 1
EcoRI G'AATT_C
Cuts at: 3127 3505 3127
Size: 378 5440
EcoRV GAT'ATC
Cuts at: 2664 2664
Size: 5818
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2612 2612
Size: 5818
FspI TGC'GCA
Cuts at: 4324 4324
Size: 5818
HaeII r_GCGC'y
Cuts at: 1678 2120 4965 1678
Size: 442 2845 2531
Fragments arranged by size:
2845 2531 442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2413 2445 2987 3012 3019
Size: 32 135 32 841 32 542 25 7
Cuts at: 3019 3044 3095 3127 4459 4491 4533 4565 1373
Size: 25 51 32 1332 32 42 32 2626
Fragments arranged by size:
2626 1332 841 542 135 51 42 32
32 32 32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3162 3408 669
Size: 988 281 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5818
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KpnI G_GTAC'C
Cuts at: 2138 3143 2138
Size: 1005 4813
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 5694 5694
Size: 5818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2238 2868 4932 5116 1305
Size: 933 630 2064 184 2007
Fragments arranged by size:
2064 2007 933 630 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2986 10
Size: 54 1425 343 1154 2842
Fragments arranged by size:
2842 1425 1154 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2881 3776 4135 4294 518
Size: 648 1715 895 359 159 2042
Fragments arranged by size:
2042 1715 895 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2774 2833 3234 3911 5059 5304 1606
Size: 1168 59 401 677 1148 245 2120
Fragments arranged by size:
2120 1168 1148 677 401 245 59
NcoI C'CATG_G
Cuts at: 513 1065 2004 2649 513
Size: 552 939 645 3682
Fragments arranged by size:
3682 939 645 552
NdeI CA'TA_TG
Cuts at: 387 2328 387
Size: 1941 3877
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 2950 3176 4721 1132
Size: 97 1721 226 1545 2229
Fragments arranged by size:
2229 1721 1545 226 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5818
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2572 2650 2887 1108
Size: 1464 78 237 4039
Fragments arranged by size:
4039 1464 237 78
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2307 2821 3023 2307
Size: 514 202 5102
Fragments arranged by size:
5102 514 202
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PspOMI G'GGCC_C
Cuts at: 2100 2100
Size: 5818
PstI C_TGCA'G
Cuts at: 830 2853 3042 3170 830
Size: 2023 189 128 3478
Fragments arranged by size:
3478 2023 189 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
PvuII CAG'CTG
Cuts at: 2774 2833 2774
Size: 59 5759
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SanDI GG'GwC_CC
Cuts at: 2307 2307
Size: 5818
SbfI CC_TGCA'GG
Cuts at: 3042 3170 3042
Size: 128 5690
ScaI AGT'ACT
Cuts at: 2367 4066 5778 2367
Size: 1699 1712 2407
Fragments arranged by size:
2407 1712 1699
SfcI C'TryA_G
Cuts at: 826 1045 2293 2849 3038 3166 4301 4982 5173
Size: 219 1248 556 189 128 1135 681 191
Cuts at: 5173 826
Size: 1471
Fragments arranged by size:
1471 1248 1135 681 556 219 191 189
128
SfiI GGCCn_nnn'nGGCC
Cuts at: 2099 2099
Size: 5818
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 3145 1872
Size: 1273 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2496 3922 4823 5085 820
Size: 197 926 553 1426 901 262 1553
Fragments arranged by size:
1553 1426 926 901 553 262 197
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 2707 152
Size: 2555 3263
SphI G_CATG'C
Cuts at: 1132 3176 1132
Size: 2044 3774
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5818
StyI C'CwwG_G
Cuts at: 513 1065 2004 2649 3257 513
Size: 552 939 645 608 3074
Fragments arranged by size:
3074 939 645 608 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3845 3862 4015 4200 4645 325
Size: 1255 2265 17 153 185 445 1498
Fragments arranged by size:
2265 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2365 2590 2742
Size: 274 80 33 51 1443 388 225 152
Cuts at: 2742 2905 4064 5776 96
Size: 163 1159 1712 138
Fragments arranged by size:
1712 1443 1159 388 274 225 163 152
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2231 2263 5778 5810 2231
Size: 32 3515 32 2239
Fragments arranged by size:
3515 2239 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5818
XbaI T'CTAG_A
Cuts at: 2278 2623 3154 2278
Size: 345 531 4942
Fragments arranged by size:
4942 531 345
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5818
XmaI C'CCGG_G
Cuts at: 1870 3143 1870
Size: 1273 4545
XmnI GAAnn'nnTTC
Cuts at: 2451 3947 2451
Size: 1496 4322
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfoI SrfI SwaI XcmI