Restriction map for
pFN21AB7651
(Circular) (Six-base) MAPSORT of: pFN21AB7651.seq Check: 3995 from: 1 to: 5695
pFN21AB7651 5695 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3758 5142 5410 1632
Size: 2126 1384 268 1917
Fragments arranged by size:
2126 1917 1384 268
AfeI AGC'GCT
Cuts at: 2386 2660 2386
Size: 274 5421
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 4594 5571 1203
Size: 3391 977 1327
Fragments arranged by size:
3391 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 2975 1166
Size: 1809 3886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2846 1184
Size: 1662 4033
AlwNI CAG_nnn'CTG
Cuts at: 2452 2875 2929 3438 5010 2452
Size: 423 54 509 1572 3137
Fragments arranged by size:
3137 1572 509 423 54
ApaLI G'TGCA_C
Cuts at: 2897 3752 4908 2897
Size: 855 1156 3684
Fragments arranged by size:
3684 1156 855
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 3020 1870
Size: 73 92 985 4545
Fragments arranged by size:
4545 985 92 73
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2273 2369 2404 3016
Size: 325 217 627 375 111 96 35 612
Cuts at: 3016 4471 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 612 375 325 217 111
96 35
BanII G_rGCy'C
Cuts at: 1391 2052 2189 3014 1391
Size: 661 137 825 4072
Fragments arranged by size:
4072 825 661 137
BbeI G_GCGC'C
Cuts at: 2166 2277 2166
Size: 111 5584
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3886 3920 1903
Size: 34 1949 34 3678
Fragments arranged by size:
3678 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2253 2262 2705
Size: 107 122 71 631 605 581 9 443
Cuts at: 2705 4306 136
Size: 1601 1525
Fragments arranged by size:
1601 1525 631 605 581 443 122 107
71 9
BglII A'GATC_T
Cuts at: 2640 5690 2640
Size: 3050 2645
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2380 2478 2901 3756 4912 1790
Size: 590 98 423 855 1156 2573
Fragments arranged by size:
2573 1156 855 590 423 98
BmgBI CAC'GTC
Cuts at: 1318 2622 2973 1318
Size: 1304 351 4040
Fragments arranged by size:
4040 1304 351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2229 2253 2672 4355 1239
Size: 869 121 24 419 1683 2579
Fragments arranged by size:
2579 1683 869 419 121 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3820 4685 4983 3820
Size: 865 298 4532
Fragments arranged by size:
4532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 2274 2499 2594
Size: 53 83 186 936 630 111 225 95
Cuts at: 2594 2981 3502 3884 4519 275
Size: 387 521 382 635 1451
Fragments arranged by size:
1451 936 635 630 521 387 382 225
186 111 95 83 53
BsaWI w'CCGG_w
Cuts at: 2589 4128 4526 4800 4947 5358 2589
Size: 1539 398 274 147 411 2926
Fragments arranged by size:
2926 1539 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2226 1186
Size: 1040 4655
BseYI C'CCAG_C
Cuts at: 2445 4898 5394 2445
Size: 2453 496 2746
Fragments arranged by size:
2746 2453 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2196 2691 3145 3906 4934 1540
Size: 275 186 195 495 454 761 1028 2301
Fragments arranged by size:
2301 1028 761 495 454 275 195 186
BsiHKAI G_wGCw'C
Cuts at: 2013 2901 3756 3841 4912 2013
Size: 888 855 85 1071 2796
Fragments arranged by size:
2796 1071 888 855 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
BsmBI CGTCTCn'nnnn_
Cuts at: 2767 2767
Size: 5695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2052 2189 2380 2478 2901 3014
Size: 399 223 39 137 191 98 423 113
Cuts at: 3014 3756 3841 4912 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 423 399 223 191 137
113 98 85 39
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2725 3050 844
Size: 1881 325 3489
Fragments arranged by size:
3489 1881 325
BsrBI CCG'CTC
Cuts at: 1394 1823 2216 2422 3582 1394
Size: 429 393 206 1160 3507
Fragments arranged by size:
3507 1160 429 393 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2297 2407 2459 2757 4339 4526 1700
Size: 110 487 110 52 298 1582 187 2869
Fragments arranged by size:
2869 1582 487 298 187 110 110 52
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 2695 1925
Size: 770 4925
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2929 794
Size: 2135 3560
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 2573 1184
Size: 1389 4306
BstYI r'GATC_y
Cuts at: 1972 2073 2640 3025 3392 3777 3794 5246 5332
Size: 101 567 385 367 385 17 1452 86
Cuts at: 5332 5344 5690 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 567 385 385 367 346 101
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2190 2433 2936 513
Size: 552 939 186 243 503 3272
Fragments arranged by size:
3272 939 552 503 243 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 3211 4005 4025 3211
Size: 794 20 4881
Fragments arranged by size:
4881 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2454 2688
Size: 54 1425 50 275 18 363 261 234
Cuts at: 2688 2706 3142 4031 5660 8
Size: 18 436 889 1629 43
Fragments arranged by size:
1629 1425 889 436 363 275 261 234
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2193 2688 3142 1537
Size: 275 381 495 454 4090
Fragments arranged by size:
4090 495 454 381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2852 2918 3625 1353
Size: 1499 66 707 3423
Fragments arranged by size:
3423 1499 707 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2776 4282 4656 4802 387
Size: 1064 138 1187 1506 374 146 1280
Fragments arranged by size:
1506 1280 1187 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2229 2253 2672 3758 4355 5142
Size: 393 476 121 24 419 1086 597 787
Cuts at: 5142 5410 1239
Size: 268 1524
Fragments arranged by size:
1524 1086 787 597 476 419 393 268
121 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 2708
Size: 5695
EcoO109I rG'GnC_Cy
Cuts at: 2108 2220 2237 2288 2108
Size: 112 17 51 5515
Fragments arranged by size:
5515 112 51 17
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FspI TGC'GCA
Cuts at: 2733 4201 2733
Size: 1468 4227
HaeII r_GCGC'y
Cuts at: 1678 2166 2277 2388 2662 2717 4842 1678
Size: 488 111 111 274 55 2125 2531
Fragments arranged by size:
2531 2125 488 274 111 111 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4336 4368 4410 4442 1373
Size: 32 135 32 2764 32 42 32 2626
Fragments arranged by size:
2764 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2503 3039 3285 669
Size: 988 281 565 536 246 3079
Fragments arranged by size:
3079 988 565 536 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KasI G'GCGC_C
Cuts at: 2162 2273 2162
Size: 111 5584
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2137 4809 4993 1305
Size: 832 2672 184 2007
Fragments arranged by size:
2672 2007 832 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2456 2708 10
Size: 54 1425 343 624 252 2997
Fragments arranged by size:
2997 1425 624 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2975 3653 4012 4171 518
Size: 648 1809 678 359 159 2042
Fragments arranged by size:
2042 1809 678 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2192 2364 2875 2882 3111 3788 4936 5181
Size: 586 172 511 7 229 677 1148 245
Cuts at: 5181 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 586 511 245 229 172
7
NaeI GCC'GGC
Cuts at: 2409 2409
Size: 5695
NarI GG'CG_CC
Cuts at: 2163 2274 2163
Size: 111 5584
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4204
Fragments arranged by size:
4204 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NgoMIV G'CCGG_C
Cuts at: 2407 2407
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 2193 3142 2193
Size: 949 4746
NspI r_CATG'y
Cuts at: 1132 1229 3053 4598 1132
Size: 97 1824 1545 2229
Fragments arranged by size:
2229 1824 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5695
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 1108
Size: 1344 4351
PfoI T'CCnGG_A
Cuts at: 2124 2124
Size: 5695
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2108 2237 2288 2108
Size: 129 51 5515
Fragments arranged by size:
5515 129 51
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PstI C_TGCA'G
Cuts at: 830 2282 2640 2835 2925 2934 3047 830
Size: 1452 358 195 90 9 113 3478
Fragments arranged by size:
3478 1452 358 195 113 90 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
SacII CC_GC'GG
Cuts at: 2193 2193
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SanDI GG'GwC_CC
Cuts at: 2108 2108
Size: 5695
SapI GCTCTTCn'nnn_
Cuts at: 2852 2918 2852
Size: 66 5629
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SexAI A'CCwGG_T
Cuts at: 2744 2744
Size: 5695
SfcI C'TryA_G
Cuts at: 826 1045 2278 2636 2831 2921 2930 3043 4178
Size: 219 1233 358 195 90 9 113 1135
Cuts at: 4178 4859 5050 826
Size: 681 191 1471
Fragments arranged by size:
1471 1233 1135 681 358 219 195 191
113 90 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2253 2253
Size: 5695
SfoI GGC'GCC
Cuts at: 2164 2275 2164
Size: 111 5584
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 2037 3022 1872
Size: 165 985 4545
Fragments arranged by size:
4545 985 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3799 4700 4962 820
Size: 197 926 1856 901 262 1553
Fragments arranged by size:
1856 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2849 1517
Size: 1332 4363
StyI C'CwwG_G
Cuts at: 513 1065 2004 2143 2322 2709 3134 513
Size: 552 939 139 179 387 425 3074
Fragments arranged by size:
3074 939 552 425 387 179 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2741 3722 3739 3892 4077 4522 325
Size: 1255 1161 981 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1161 981 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3941 5653 96
Size: 274 80 33 51 1443 1964 1712 138
Fragments arranged by size:
1964 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 2035 3020 1870
Size: 165 985 4545
Fragments arranged by size:
4545 985 165
XmnI GAAnn'nnTTC
Cuts at: 2660 3824 2660
Size: 1164 4531
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SrfI SwaI