Restriction map for
pFN21AB7648
(Circular) (Six-base) MAPSORT of: pFN21AB7648.seq Check: 8416 from: 1 to: 5881
pFN21AB7648 5881 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 3137 3202 3137
Size: 65 5816
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 3944 5328 5596 1632
Size: 464 1848 1384 268 1917
Fragments arranged by size:
1917 1848 1384 464 268
AfeI AGC'GCT
Cuts at: 2348 2348
Size: 5881
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 3028 4780 5757 1203
Size: 1825 1752 977 1327
Fragments arranged by size:
1825 1752 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 3624 5196 3624
Size: 1572 4309
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 3190 3416 3568 5677 1493
Size: 1697 226 152 2109 1697
Fragments arranged by size:
2109 1697 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2688 3206 1870
Size: 73 745 518 4545
Fragments arranged by size:
4545 745 518 73
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3137 3202 4657 618
Size: 325 217 627 1350 65 1455 1842
Fragments arranged by size:
1842 1455 1350 627 325 217 65
BanII G_rGCy'C
Cuts at: 1391 3200 1391
Size: 1809 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2254 2705 928
Size: 971 355 451 4104
Fragments arranged by size:
4104 971 451 355
BbvCI CC'TCA_GC
Cuts at: 2109 2109
Size: 5881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4072 4106 1903
Size: 34 2135 34 3678
Fragments arranged by size:
3678 2135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2753 4492 136
Size: 107 122 71 631 605 1081 1739 1525
Fragments arranged by size:
1739 1525 1081 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 3942 5098 1790
Size: 2152 1156 2573
Fragments arranged by size:
2573 2152 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2335 2840 3612
Size: 416 53 83 399 24 918 505 772
Cuts at: 3612 4570 442
Size: 958 1753
Fragments arranged by size:
1753 958 918 772 505 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3082 3097 3082
Size: 15 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2576 2759 4541 1239
Size: 1337 183 1782 2579
Fragments arranged by size:
2579 1782 1337 183
Bpu10I CC'TnA_GC
Cuts at: 2109 2109
Size: 5881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4006 4871 5169 4006
Size: 865 298 4718
Fragments arranged by size:
4718 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4544 882
Size: 617 3045 2219
Fragments arranged by size:
3045 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 5875 5875
Size: 5881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 4070 4705 275
Size: 53 83 186 936 2155 382 635 1451
Fragments arranged by size:
2155 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4314 4712 4986 5133 5544 4314
Size: 398 274 147 411 4651
Fragments arranged by size:
4651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 1178
Size: 30 165 30 1146 30 4480
Fragments arranged by size:
4480 1146 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 3089 1186
Size: 916 987 3978
Fragments arranged by size:
3978 987 916
BseYI C'CCAG_C
Cuts at: 2877 3008 5084 5580 2877
Size: 131 2076 496 3178
Fragments arranged by size:
3178 2076 496 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2618 1163
Size: 573 882 4426
Fragments arranged by size:
4426 882 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3942 4027 5098 3942
Size: 85 1071 4725
Fragments arranged by size:
4725 1071 85
BsmI GAATG_Cn'
Cuts at: 2340 3392 3485 2340
Size: 1052 93 4736
Fragments arranged by size:
4736 1052 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3200 3942 4027 5098 1391
Size: 399 1410 742 85 1071 2174
Fragments arranged by size:
2174 1410 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 2716 3770 5500 2716
Size: 1054 1730 3097
Fragments arranged by size:
3097 1730 1054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3236 844
Size: 2392 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2494 4376 4550 65
Size: 1183 1246 1882 174 1396
Fragments arranged by size:
1882 1396 1246 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4525 4712 1700
Size: 110 2715 187 2869
Fragments arranged by size:
2869 2715 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 3941 4953 3941
Size: 1012 4869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2762 794
Size: 1968 3913
BstBI TT'CG_AA
Cuts at: 2335 4685 2335
Size: 2350 3531
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstXI CCAn_nnnn'nTGG
Cuts at: 2320 2830 2320
Size: 510 5371
BstYI r'GATC_y
Cuts at: 1972 2013 3211 3578 3963 3980 5432 5518 5530
Size: 41 1198 367 385 17 1452 86 12
Cuts at: 5530 5876 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1198 385 367 346 86 41
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2808 3171 513
Size: 552 939 804 363 3223
Fragments arranged by size:
3223 939 804 552 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 3108 3397 4191 4211 2265
Size: 843 289 794 20 3935
Fragments arranged by size:
3935 843 794 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2851 2886 529
Size: 35 2287 35 3524
Fragments arranged by size:
3524 2287 35 35
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846
Size: 54 1425 50 275 18 1498 889 1629
Cuts at: 5846 8
Size: 43
Fragments arranged by size:
1629 1498 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2739 3811 1353
Size: 1386 1072 3423
Fragments arranged by size:
3423 1386 1072
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 2226 4468 4842 4988 387
Size: 1064 138 495 142 2242 374 146 1280
Fragments arranged by size:
2242 1280 1064 495 374 146 142 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2576 2759 3944 4541 5328 5596
Size: 393 464 480 183 1185 597 787 268
Cuts at: 5596 1239
Size: 1524
Fragments arranged by size:
1524 1185 787 597 480 464 393 268
183
EcoO109I rG'GnC_Cy
Cuts at: 2113 2113
Size: 5881
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
EcoRV GAT'ATC
Cuts at: 2602 2602
Size: 5881
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 2350 5028 1678
Size: 672 2678 2531
Fragments arranged by size:
2678 2531 672
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2572 2604 3044 3076 4522
Size: 32 135 32 1000 32 440 32 1446
Cuts at: 4522 4554 4596 4628 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1446 1000 440 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2677 3225 3471 669
Size: 988 281 739 548 246 3079
Fragments arranged by size:
3079 988 739 548 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5881
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3141 3206 3141
Size: 65 5816
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2869 4995 5179 1305
Size: 1564 2126 184 2007
Fragments arranged by size:
2126 2007 1564 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 3839 4198 4357 518
Size: 648 1120 1553 359 159 2042
Fragments arranged by size:
2042 1553 1120 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2726 3297 3974 5122 5367 1606
Size: 1120 571 677 1148 245 2120
Fragments arranged by size:
2120 1148 1120 677 571 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3171 513
Size: 552 939 1167 3223
Fragments arranged by size:
3223 1167 939 552
NdeI CA'TA_TG
Cuts at: 387 2671 387
Size: 2284 3597
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 2413 3032 3239 4784 1132
Size: 97 1184 619 207 1545 2229
Fragments arranged by size:
2229 1545 1184 619 207 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 3028 4780 3028
Size: 1752 4129
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5881
PfoI T'CCnGG_A
Cuts at: 2553 3091 2553
Size: 538 5343
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 2113
Size: 5881
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PstI C_TGCA'G
Cuts at: 830 3233 830
Size: 2403 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2726 2726
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SapI GCTCTTCn'nnn_
Cuts at: 2739 2739
Size: 5881
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 3229 4364 5045 5236 826
Size: 219 2184 1135 681 191 1471
Fragments arranged by size:
2184 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3208 1872
Size: 1336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3985 4886 5148 820
Size: 197 926 2042 901 262 1553
Fragments arranged by size:
2042 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 2307 1517
Size: 790 5091
StyI C'CwwG_G
Cuts at: 513 1065 2004 3036 3171 3320 513
Size: 552 939 1032 135 149 3074
Fragments arranged by size:
3074 1032 939 552 149 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2839 3908 3925 4078 4263 4708 325
Size: 1255 1259 1069 17 153 185 445 1498
Fragments arranged by size:
1498 1259 1255 1069 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2935 4127 5839
Size: 274 80 33 51 1443 958 1192 1712
Cuts at: 5839 96
Size: 138
Fragments arranged by size:
1712 1443 1192 958 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5841 5873 5841
Size: 32 5849
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2248 2248
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 3206 1870
Size: 1336 4545
XmnI GAAnn'nnTTC
Cuts at: 2951 4010 2951
Size: 1059 4822
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI