Restriction map for
pFN21AB7644
(Circular) (Six-base) MAPSORT of: pFN21AB7644.seq Check: 409 from: 1 to: 5875
pFN21AB7644 5875 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2346 2346
Size: 5875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2229 2331 3218 1937
Size: 292 102 887 4594
Fragments arranged by size:
4594 887 292 102
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2669 3938 5322 5590 1632
Size: 521 516 1269 1384 268 1917
Fragments arranged by size:
1917 1384 1269 521 516 268
AfeI AGC'GCT
Cuts at: 2255 2255
Size: 5875
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 4774 5751 1203
Size: 3571 977 1327
Fragments arranged by size:
3571 1327 977
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2364 1184
Size: 1180 4695
AlwNI CAG_nnn'CTG
Cuts at: 2151 2163 2468 3058 3618 5190 2151
Size: 12 305 590 560 1572 2836
Fragments arranged by size:
2836 1572 590 560 305 12
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 2390 2790 3184 3410 3562 5671 1493
Size: 897 400 394 226 152 2109 1697
Fragments arranged by size:
2109 1697 897 400 394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 3200 1870
Size: 73 1257 4545
Fragments arranged by size:
4545 1257 73
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2667 3196 4651 618
Size: 325 217 627 880 529 1455 1842
Fragments arranged by size:
1842 1455 880 627 529 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 2738 2924 3194 1391
Size: 661 686 186 270 4072
Fragments arranged by size:
4072 686 661 270 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2436 928
Size: 971 537 4367
Fragments arranged by size:
4367 971 537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2300 3767 4977 2300
Size: 1467 1210 3198
Fragments arranged by size:
3198 1467 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5875
BfrBI ATG'CAT
Cuts at: 2528 2528
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2554 4486 136
Size: 107 122 71 631 605 882 1932 1525
Fragments arranged by size:
1932 1525 882 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5870 5870
Size: 5875
BlpI GC'TnA_GC
Cuts at: 2475 2771 3292 2475
Size: 296 521 5058
Fragments arranged by size:
5058 521 296
Bme1580I G_kGCm'C
Cuts at: 1790 3936 5092 1790
Size: 2146 1156 2573
Fragments arranged by size:
2573 2146 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 2932 3606
Size: 416 53 83 399 24 666 849 674
Cuts at: 3606 4564 442
Size: 958 1753
Fragments arranged by size:
1753 958 849 674 666 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2197 2490 2531 4535 1239
Size: 958 293 41 2004 2579
Fragments arranged by size:
2579 2004 958 293 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2929 4000 4865 5163 2153
Size: 776 1071 865 298 2865
Fragments arranged by size:
2865 1071 865 776 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4538 882
Size: 617 3039 2219
Fragments arranged by size:
3039 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2560 3666 493
Size: 711 1356 1106 2702
Fragments arranged by size:
2702 1356 1106 711
BsaBI GATnn'nnATC
Cuts at: 5869 5869
Size: 5875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2522 2552 2558 2588 1178
Size: 30 165 30 1119 30 6 30 4465
Fragments arranged by size:
4465 1119 165 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2716 1186
Size: 1530 4345
BseYI C'CCAG_C
Cuts at: 2219 2673 3071 5078 5574 2219
Size: 454 398 2007 496 2520
Fragments arranged by size:
2520 2007 496 454 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2196 2324 1163
Size: 573 460 128 4714
Fragments arranged by size:
4714 573 460 128
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2738 3936 4021 5092 2738
Size: 1198 85 1071 3521
Fragments arranged by size:
3521 1198 1071 85
BsmI GAATG_Cn'
Cuts at: 2530 3386 3479 2530
Size: 856 93 4926
Fragments arranged by size:
4926 856 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2738 2924 3194 3936 4021 5092
Size: 399 262 686 186 270 742 85 1071
Cuts at: 5092 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 686 399 270 262 186
85
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 3764 5494 3764
Size: 1730 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 3230 844
Size: 1502 884 3489
Fragments arranged by size:
3489 1502 884
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3024 4370 4544 65
Size: 1183 1776 1346 174 1396
Fragments arranged by size:
1776 1396 1346 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2367 4519 4706 1700
Size: 110 557 2152 187 2869
Fragments arranged by size:
2869 2152 557 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 3935 4947 3935
Size: 1012 4863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2268 2277 2361 794
Size: 1474 9 84 4308
Fragments arranged by size:
4308 1474 84 9
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstXI CCAn_nnnn'nTGG
Cuts at: 2096 2509 3144 2096
Size: 413 635 4827
Fragments arranged by size:
4827 635 413
BstYI r'GATC_y
Cuts at: 1972 3205 3572 3957 3974 5426 5512 5524 5870
Size: 1233 367 385 17 1452 86 12 346
Cuts at: 5870 1972
Size: 1977
Fragments arranged by size:
1977 1452 1233 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2028 2379 2877 3066 513
Size: 552 963 351 498 189 3322
Fragments arranged by size:
3322 963 552 498 351 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 2341 3112 3391 4185 4205 2341
Size: 771 279 794 20 4011
Fragments arranged by size:
4011 794 771 279 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2286 2321 529
Size: 35 1722 35 4083
Fragments arranged by size:
4083 1722 35 35
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 4882 809
Size: 507 1284 2282 1802
Fragments arranged by size:
2282 1802 1284 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 2806 3322
Size: 54 1425 50 275 18 546 430 516
Cuts at: 3322 4211 5840 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 546 516 430 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3805 1353
Size: 2452 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 2865 4462 4836 4982 387
Size: 1064 138 624 652 1597 374 146 1280
Fragments arranged by size:
1597 1280 1064 652 624 374 146 138
EcoICRI GAG'CTC
Cuts at: 2736 2736
Size: 5875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2197 2490 2531 2669 3938 4535
Size: 393 521 44 293 41 138 1269 597
Cuts at: 4535 5322 5590 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1269 787 597 521 393 293 268
138 44 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 2651 2402
Size: 249 5626
EcoO109I rG'GnC_Cy
Cuts at: 2137 3077 2137
Size: 940 4935
EcoRI G'AATT_C
Cuts at: 2390 3184 3562 2390
Size: 794 378 4703
Fragments arranged by size:
4703 794 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 5875
FspI TGC'GCA
Cuts at: 3030 4381 3030
Size: 1351 4524
HaeII r_GCGC'y
Cuts at: 1678 2257 5022 1678
Size: 579 2765 2531
Fragments arranged by size:
2765 2531 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2558 2590 4516 4548 4590
Size: 32 135 32 986 32 1926 32 42
Cuts at: 4590 4622 1373
Size: 32 2626
Fragments arranged by size:
2626 1926 986 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5875
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3152 4989 5173 1305
Size: 1847 1837 184 2007
Fragments arranged by size:
2007 1847 1837 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 2808 10
Size: 54 1425 343 546 430 3077
Fragments arranged by size:
3077 1425 546 430 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3833 4192 4351 518
Size: 648 2667 359 159 2042
Fragments arranged by size:
2667 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2194 2374 2509 2572 2673 3291 3968
Size: 424 164 180 135 63 101 618 677
Cuts at: 3968 5116 5361 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 618 424 245 180 164
135 101 63
NcoI C'CATG_G
Cuts at: 513 1065 2877 3066 513
Size: 552 1812 189 3322
Fragments arranged by size:
3322 1812 552 189
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5875
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NsiI A_TGCA'T
Cuts at: 2530 2530
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 3035 3233 4778 1132
Size: 97 1806 198 1545 2229
Fragments arranged by size:
2229 1806 1545 198 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PfoI T'CCnGG_A
Cuts at: 2052 2115 2139 2052
Size: 63 24 5788
Fragments arranged by size:
5788 63 24
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PmlI CAC'GTG
Cuts at: 2560 2560
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2444 2476 3944 3976 5457 5489 2444
Size: 32 1468 32 1481 32 2830
Fragments arranged by size:
2830 1481 1468 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 3077 2137
Size: 940 4935
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5875
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PstI C_TGCA'G
Cuts at: 830 2743 3227 830
Size: 1913 484 3478
Fragments arranged by size:
3478 1913 484
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 2194 2374 2509 2572 2194
Size: 180 135 63 5497
Fragments arranged by size:
5497 180 135 63
SacI G_AGCT'C
Cuts at: 2738 2738
Size: 5875
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SanDI GG'GwC_CC
Cuts at: 3077 3077
Size: 5875
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SfcI C'TryA_G
Cuts at: 826 1045 2739 3223 4358 5039 5230 826
Size: 219 1694 484 1135 681 191 1471
Fragments arranged by size:
1694 1471 1135 681 484 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3202 1872
Size: 1330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 2908 3979 4880 5142 820
Size: 197 926 225 740 1071 901 262 1553
Fragments arranged by size:
1553 1071 926 901 740 262 225 197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3035 3233 1132
Size: 1903 198 3774
Fragments arranged by size:
3774 1903 198
SspI AAT'ATT
Cuts at: 5 52 3799 5
Size: 47 3747 2081
Fragments arranged by size:
3747 2081 47
StuI AGG'CCT
Cuts at: 1517 2589 1517
Size: 1072 4803
StyI C'CwwG_G
Cuts at: 513 1065 2877 3066 3314 513
Size: 552 1812 189 248 3074
Fragments arranged by size:
3074 1812 552 248 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3902 3919 4072 4257 4702 325
Size: 1255 2322 17 153 185 445 1498
Fragments arranged by size:
2322 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4121 5833 96
Size: 274 80 33 51 1443 2144 1712 138
Fragments arranged by size:
2144 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5835 5867 5835
Size: 32 5843
Tth111I GACn'n_nGTC
Cuts at: 1316 2327 1316
Size: 1011 4864
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 3200 1870
Size: 1330 4545
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI
PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI
SwaI XcmI