Restriction map for
pFN21AB7643
(Circular) (Six-base) MAPSORT of: pFN21AB7643.seq Check: 9907 from: 1 to: 5602
pFN21AB7643 5602 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2365 2365
Size: 5602
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 5049 5317 1632
Size: 2033 1384 268 1917
Fragments arranged by size:
2033 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 2223 4501 5478 1203
Size: 1020 2278 977 1327
Fragments arranged by size:
2278 1327 1020 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 2433 2642 2699 1166
Size: 1267 209 57 4069
Fragments arranged by size:
4069 1267 209 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AlwNI CAG_nnn'CTG
Cuts at: 3345 4917 3345
Size: 1572 4030
ApaLI G'TGCA_C
Cuts at: 2720 3659 4815 2720
Size: 939 1156 3507
Fragments arranged by size:
3507 1156 939
ApoI r'AATT_y
Cuts at: 1493 2342 2911 3137 3289 5398 1493
Size: 849 569 226 152 2109 1697
Fragments arranged by size:
2109 1697 849 569 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2309 2927 1870
Size: 73 271 95 618 4545
Fragments arranged by size:
4545 618 271 95 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2923 4378 618
Size: 325 217 627 1136 1455 1842
Fragments arranged by size:
1842 1455 1136 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2425 2921 1391
Size: 1034 496 4072
Fragments arranged by size:
4072 1034 496
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2270 4213 136
Size: 107 122 71 631 605 598 1943 1525
Fragments arranged by size:
1943 1525 631 605 598 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 2322 2393 2724 3663 4819 1790
Size: 532 71 331 939 1156 2573
Fragments arranged by size:
2573 1156 939 532 331 71
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3333 4291 442
Size: 416 53 83 399 24 1916 958 1753
Fragments arranged by size:
1916 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 4262 1239
Size: 806 2217 2579
Fragments arranged by size:
2579 2217 806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2224 3393 493
Size: 711 1020 1169 2702
Fragments arranged by size:
2702 1169 1020 711
BsaBI GATnn'nnATC
Cuts at: 2012 5596 2012
Size: 3584 2018
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 3791 4426 275
Size: 53 83 186 936 1876 382 635 1451
Fragments arranged by size:
1876 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5377
Fragments arranged by size:
5377 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5602
BseYI C'CCAG_C
Cuts at: 2841 4805 5301 2841
Size: 1964 496 3142
Fragments arranged by size:
3142 1964 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2638 2716 1163
Size: 573 902 78 4049
Fragments arranged by size:
4049 902 573 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2419 3052 3813 4841 1540
Size: 275 186 418 633 761 1028 2301
Fragments arranged by size:
2301 1028 761 633 418 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 3663 3748 4819 2724
Size: 939 85 1071 3507
Fragments arranged by size:
3507 1071 939 85
BsmI GAATG_Cn'
Cuts at: 2451 3113 3206 2451
Size: 662 93 4847
Fragments arranged by size:
4847 662 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2735 2735
Size: 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2393 2425 2724 2921 3663 3748
Size: 399 532 71 32 299 197 742 85
Cuts at: 3748 4819 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 532 399 299 197 85
71 32
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 2365 2957 844
Size: 1421 100 592 3489
Fragments arranged by size:
3489 1421 592 100
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 3489 1394
Size: 429 221 1445 3507
Fragments arranged by size:
3507 1445 429 221
BsrDI GCAATG_nn'
Cuts at: 65 1248 2590 4097 4271 65
Size: 1183 1342 1507 174 1396
Fragments arranged by size:
1507 1396 1342 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 2382 2535 96
Size: 2286 153 3163
Fragments arranged by size:
3163 2286 153
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2932 3299 3684 3701 5153 5239 5251 5597
Size: 960 367 385 17 1452 86 12 346
Cuts at: 5597 1972
Size: 1977
Fragments arranged by size:
1977 1452 960 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5050
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2122 487
Size: 683 56 896 3967
Fragments arranged by size:
3967 896 683 56
BtsI GCAGTG_nn'
Cuts at: 2284 2322 2428 2440 3118 3912 3932 2284
Size: 38 106 12 678 794 20 3954
Fragments arranged by size:
3954 794 678 106 38 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2821 3049 3938
Size: 54 1425 50 275 18 991 228 889
Cuts at: 3938 5567 8
Size: 1629 43
Fragments arranged by size:
1629 1425 991 889 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2687 2763 3532 1353
Size: 1334 76 769 3423
Fragments arranged by size:
3423 1334 769 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 3665 4262 5049 5317 1239
Size: 393 413 1620 597 787 268 1524
Fragments arranged by size:
1620 1524 787 597 413 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2674 2712 2674
Size: 38 5564
EcoRI G'AATT_C
Cuts at: 2342 2911 3289 2342
Size: 569 378 4655
Fragments arranged by size:
4655 569 378
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 4749 1678
Size: 3071 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2197 2229 4243 4275 4317
Size: 32 135 32 625 32 2014 32 42
Cuts at: 4317 4349 1373
Size: 32 2626
Fragments arranged by size:
2626 2014 625 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 2946 3192 669
Size: 988 281 547 461 246 3079
Fragments arranged by size:
3079 988 547 461 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 2756 3201 2756
Size: 445 5157
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2823 10
Size: 54 1425 343 991 2789
Fragments arranged by size:
2789 1425 991 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 2570 2642 2699 3560 3919 4078
Size: 648 1267 137 72 57 861 359 159
Cuts at: 4078 518
Size: 2042
Fragments arranged by size:
2042 1267 861 648 359 159 137 72
57
MspA1I CmG'CkG
Cuts at: 1606 3018 3695 4843 5088 1606
Size: 1412 677 1148 245 2120
Fragments arranged by size:
2120 1412 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5050
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2960 4505 1132
Size: 97 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2478 1348
Size: 1130 4472
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2896 1108
Size: 1788 3814
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2712 2712
Size: 5602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2405 2435 2954 830
Size: 1575 30 519 3478
Fragments arranged by size:
3478 1575 519 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SanDI GG'GwC_CC
Cuts at: 2712 2712
Size: 5602
SapI GCTCTTCn'nnn_
Cuts at: 2687 2687
Size: 5602
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 2516 3850 5562 2516
Size: 1334 1712 2556
Fragments arranged by size:
2556 1712 1334
SexAI A'CCwGG_T
Cuts at: 2156 2156
Size: 5602
SfcI C'TryA_G
Cuts at: 826 1045 2401 2431 2950 4085 4766 4957 826
Size: 219 1356 30 519 1135 681 191 1471
Fragments arranged by size:
1471 1356 1135 681 519 219 191 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 2270 2270
Size: 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2311 2929 1872
Size: 439 618 4545
Fragments arranged by size:
4545 618 439
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2214 3706 4607 4869 820
Size: 197 926 271 1492 901 262 1553
Fragments arranged by size:
1553 1492 926 901 271 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2869 3041 513
Size: 552 1804 172 3074
Fragments arranged by size:
3074 1804 552 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 2433 2650 3629 3646 3799 3984
Size: 1255 456 397 217 979 17 153 185
Cuts at: 3984 4429 325
Size: 445 1498
Fragments arranged by size:
1498 1255 979 456 445 397 217 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2382 2514 2535
Size: 274 80 33 51 1443 405 132 21
Cuts at: 2535 3848 5560 96
Size: 1313 1712 138
Fragments arranged by size:
1712 1443 1313 405 274 138 132 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 2214 1943
Size: 271 5331
XmaI C'CCGG_G
Cuts at: 1870 2309 2927 1870
Size: 439 618 4545
Fragments arranged by size:
4545 618 439
XmnI GAAnn'nnTTC
Cuts at: 2516 3731 2516
Size: 1215 4387
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SfoI SrfI
SwaI XcmI