Restriction map for
pFN21AB7639
(Circular) (Six-base) MAPSORT of: pFN21AB7639.seq Check: 7617 from: 1 to: 5683
pFN21AB7639 5683 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2021 3026 1937
Size: 84 1005 4594
Fragments arranged by size:
4594 1005 84
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2595 3746 5130 5398 1632
Size: 963 1151 1384 268 1917
Fragments arranged by size:
1917 1384 1151 963 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2694 4582 5559 1203
Size: 1491 1888 977 1327
Fragments arranged by size:
1888 1491 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 2714 1166
Size: 1548 4135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2638 1184
Size: 1454 4229
AlwNI CAG_nnn'CTG
Cuts at: 2677 2827 3426 4998 2677
Size: 150 599 1572 3362
Fragments arranged by size:
3362 1572 599 150
ApaI G_GGCC'C
Cuts at: 2191 2530 2191
Size: 339 5344
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2425 2992 3218 3370 5479 1493
Size: 932 567 226 152 2109 1697
Fragments arranged by size:
2109 1697 932 567 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2184 3008 1870
Size: 73 241 824 4545
Fragments arranged by size:
4545 824 241 73
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 2322 2357 2504 2810
Size: 325 217 627 378 157 35 147 306
Cuts at: 2810 2928 2964 3004 4459 618
Size: 118 36 40 1455 1842
Fragments arranged by size:
1842 1455 627 378 325 306 217 157
147 118 40 36 35
BanII G_rGCy'C
Cuts at: 1391 2136 2191 2208 2530 2559 2741 2772 3002
Size: 745 55 17 322 29 182 31 230
Cuts at: 3002 1391
Size: 4072
Fragments arranged by size:
4072 745 322 230 182 55 31 29
17
BbeI G_GCGC'C
Cuts at: 2169 2326 2814 2932 2968 2169
Size: 157 488 118 36 4884
Fragments arranged by size:
4884 488 157 118 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4294 136
Size: 107 122 71 631 605 2622 1525
Fragments arranged by size:
2622 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 2179 3100 2179
Size: 921 4762
Bme1580I G_kGCm'C
Cuts at: 1790 2191 2530 3744 4900 1790
Size: 401 339 1214 1156 2573
Fragments arranged by size:
2573 1214 1156 401 339
BmgBI CAC'GTC
Cuts at: 1318 2695 1318
Size: 1377 4306
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2490 3414 4372
Size: 416 53 83 399 24 1073 924 958
Cuts at: 4372 442
Size: 1753
Fragments arranged by size:
1753 1073 958 924 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2234 2240 2322 2347 4343 1239
Size: 911 84 6 82 25 1996 2579
Fragments arranged by size:
2579 1996 911 84 82 25 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2710 3808 4673 4971 2710
Size: 1098 865 298 3422
Fragments arranged by size:
3422 1098 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4346 882
Size: 617 2847 2219
Fragments arranged by size:
2847 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 2323 2784 2811
Size: 53 83 186 936 633 157 461 27
Cuts at: 2811 2929 2965 3490 3872 4507 275
Size: 118 36 525 382 635 1451
Fragments arranged by size:
1451 936 635 633 525 461 382 186
157 118 83 53 36 27
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 1178
Size: 30 165 30 633 30 4795
Fragments arranged by size:
4795 633 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2963 1186
Size: 871 906 3906
Fragments arranged by size:
3906 906 871
BseYI C'CCAG_C
Cuts at: 2037 2260 2530 2541 4886 5382 2037
Size: 223 270 11 2345 496 2338
Fragments arranged by size:
2345 2338 496 270 223 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2136 2208 2772 3744 3829 4900 2136
Size: 72 564 972 85 1071 2919
Fragments arranged by size:
2919 1071 972 564 85 72
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2191 2208 2530 2559 2741 2772
Size: 399 346 55 17 322 29 182 31
Cuts at: 2772 3002 3744 3829 4900 1391
Size: 230 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 346 322 230 182
85 55 31 29 17
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2304 3038 844
Size: 1415 45 734 3489
Fragments arranged by size:
3489 1415 734 45
BsrBI CCG'CTC
Cuts at: 1394 1823 3570 1394
Size: 429 1747 3507
Fragments arranged by size:
3507 1747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2325 2490 2925 4327 4514 1700
Size: 110 515 165 435 1402 187 2869
Fragments arranged by size:
2869 1402 515 435 187 165 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 2312 1925
Size: 387 5296
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2272 2827 794
Size: 1478 555 3650
Fragments arranged by size:
3650 1478 555
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678
Size: 1041 367 385 17 1452 86 12 346
Cuts at: 5678 1972
Size: 1977
Fragments arranged by size:
1977 1452 1041 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2227 513
Size: 552 939 223 3969
Fragments arranged by size:
3969 939 552 223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 2721 3199 3993 4013 2240
Size: 481 478 794 20 3910
Fragments arranged by size:
3910 794 481 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2327 2923 3130
Size: 54 1425 50 275 18 497 596 207
Cuts at: 3130 4019 5648 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 596 497 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2374 3613 1353
Size: 1021 1239 3423
Fragments arranged by size:
3423 1239 1021
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2494 2533 4270 4644 4790 387
Size: 1064 138 905 39 1737 374 146 1280
Fragments arranged by size:
1737 1280 1064 905 374 146 138 39
EcoICRI GAG'CTC
Cuts at: 2134 2206 2770 2134
Size: 72 564 5047
Fragments arranged by size:
5047 564 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2234 2240 2322 2347 2595 3746
Size: 393 518 84 6 82 25 248 1151
Cuts at: 3746 4343 5130 5398 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1151 787 597 518 393 268 248
84 82 25 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2819 2846 2858 2819
Size: 27 12 5644
Fragments arranged by size:
5644 27 12
EcoO109I rG'GnC_Cy
Cuts at: 2526 2527 2731 2824 2851 2526
Size: 1 204 93 27 5358
Fragments arranged by size:
5358 204 93 27 1
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FseI GG_CCGG'CC
Cuts at: 2494 2494
Size: 5683
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 2012 2169 2227 2326 2678 2814 2932 2968
Size: 334 157 58 99 352 136 118 36
Cuts at: 2968 4830 1678
Size: 1862 2531
Fragments arranged by size:
2531 1862 352 334 157 136 118 99
58 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2723 2755 4324 4356 4398
Size: 32 135 32 1151 32 1569 32 42
Cuts at: 4398 4430 1373
Size: 32 2626
Fragments arranged by size:
2626 1569 1151 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2022 3027 3273 669
Size: 988 281 84 1005 246 3079
Fragments arranged by size:
3079 1005 988 281 246 84
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KasI G'GCGC_C
Cuts at: 2165 2322 2810 2928 2964 2165
Size: 157 488 118 36 4884
Fragments arranged by size:
4884 488 157 118 36
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2714 3641 4000 4159 518
Size: 648 1548 927 359 159 2042
Fragments arranged by size:
2042 1548 927 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2196 2229 2476 2689 3099 3776 4924 5169
Size: 590 33 247 213 410 677 1148 245
Cuts at: 5169 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 590 410 247 245 213
33
NaeI GCC'GGC
Cuts at: 2327 2492 2927 2327
Size: 165 435 5083
Fragments arranged by size:
5083 435 165
NarI GG'CG_CC
Cuts at: 2166 2323 2811 2929 2965 2166
Size: 157 488 118 36 4884
Fragments arranged by size:
4884 488 157 118 36
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4192
Fragments arranged by size:
4192 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NgoMIV G'CCGG_C
Cuts at: 2325 2490 2925 2325
Size: 165 435 5083
Fragments arranged by size:
5083 435 165
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 3041 4586 1132
Size: 97 1812 1545 2229
Fragments arranged by size:
2229 1812 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5683
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2677 1108
Size: 1569 4114
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2731 2731
Size: 5683
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PspOMI G'GGCC_C
Cuts at: 2187 2526 2187
Size: 339 5344
PstI C_TGCA'G
Cuts at: 830 2823 3035 830
Size: 1993 212 3478
Fragments arranged by size:
3478 1993 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2689 2689
Size: 5683
SacI G_AGCT'C
Cuts at: 2136 2208 2772 2136
Size: 72 564 5047
Fragments arranged by size:
5047 564 72
SacII CC_GC'GG
Cuts at: 2230 2230
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 2020 3025 1936
Size: 84 1005 4594
Fragments arranged by size:
4594 1005 84
SapI GCTCTTCn'nnn_
Cuts at: 2374 2374
Size: 5683
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 2621 3931 5643 2621
Size: 1310 1712 2661
Fragments arranged by size:
2661 1712 1310
SfcI C'TryA_G
Cuts at: 826 1045 2347 2819 3031 4166 4847 5038 826
Size: 219 1302 472 212 1135 681 191 1471
Fragments arranged by size:
1471 1302 1135 681 472 219 212 191
SfoI GGC'GCC
Cuts at: 2167 2324 2812 2930 2966 2167
Size: 157 488 118 36 4884
Fragments arranged by size:
4884 488 157 118 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 2186 3010 1872
Size: 314 824 4545
Fragments arranged by size:
4545 824 314
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2725 3787 4688 4950 820
Size: 197 926 782 1062 901 262 1553
Fragments arranged by size:
1553 1062 926 901 782 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SrfI GCCC'GGGC
Cuts at: 2186 2186
Size: 5683
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 2978 1517
Size: 1461 4222
StyI C'CwwG_G
Cuts at: 513 1065 2004 2640 3122 513
Size: 552 939 636 482 3074
Fragments arranged by size:
3074 939 636 552 482
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2132 3710 3727 3880 4065 4510 325
Size: 1255 552 1578 17 153 185 445 1498
Fragments arranged by size:
1578 1498 1255 552 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2619 3929 5641
Size: 274 80 33 51 1443 642 1310 1712
Cuts at: 5641 96
Size: 138
Fragments arranged by size:
1712 1443 1310 642 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 5643 5675 2036
Size: 32 3575 32 2044
Fragments arranged by size:
3575 2044 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 2184 3008 1870
Size: 314 824 4545
Fragments arranged by size:
4545 824 314
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FspAI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SanDI SexAI SfiI SwaI XcmI